(Circular) (Six-base) MAPSORT of: pFN21AA1624.seq Check: 7226 from: 1 to: 6505 pFN21AA1624 6505 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4315 278 Size: 53 83 186 936 2779 2468 Fragments arranged by size: 2779 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3225 3848 1937 Size: 1288 623 4594 Fragments arranged by size: 4594 1288 623 Acc65I G'GTAC_C Cuts at: 2420 3826 2420 Size: 1406 5099 AclI AA'CG_TT Cuts at: 1563 4632 5005 1563 Size: 3069 373 3063 Fragments arranged by size: 3069 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2262 2428 2484 3314 3636 4568 5952 6220 Size: 630 166 56 830 322 932 1384 268 Cuts at: 6220 1632 Size: 1917 Fragments arranged by size: 1917 1384 932 830 630 322 268 166 56 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6308 AflIII A'CryG_T Cuts at: 1203 5404 6381 1203 Size: 4201 977 1327 Fragments arranged by size: 4201 1327 977 AgeI A'CCGG_T Cuts at: 5336 5336 Size: 6505 AhdI GACnn_n'nnGTC Cuts at: 5234 5234 Size: 6505 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6505 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6505 AlwNI CAG_nnn'CTG Cuts at: 2101 2417 3139 3592 4248 5820 2101 Size: 316 722 453 656 1572 2786 Fragments arranged by size: 2786 1572 722 656 453 316 ApaLI G'TGCA_C Cuts at: 4562 5718 4562 Size: 1156 5349 ApoI r'AATT_y Cuts at: 1493 2781 3638 3814 4040 4192 6301 1493 Size: 1288 857 176 226 152 2109 1697 Fragments arranged by size: 2109 1697 1288 857 226 176 152 AseI AT'TA_AT Cuts at: 160 1032 5059 160 Size: 872 4027 1606 Fragments arranged by size: 4027 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6505 AvaI C'yCGr_G Cuts at: 1870 1943 3384 3830 1870 Size: 73 1441 446 4545 Fragments arranged by size: 4545 1441 446 73 AvrII C'CTAG_G Cuts at: 2048 2048 Size: 6505 BamHI G'GATC_C Cuts at: 3835 4202 3835 Size: 367 6138 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2420 3242 3797 3826 Size: 325 217 627 230 403 822 555 29 Cuts at: 3826 5281 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 822 627 555 403 325 230 217 29 BanII G_rGCy'C Cuts at: 1391 3780 3824 1391 Size: 2389 44 4072 Fragments arranged by size: 4072 2389 44 BbeI G_GCGC'C Cuts at: 2021 3801 2021 Size: 1780 4725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2250 2528 3607 3696 928 Size: 971 351 278 1079 89 3737 Fragments arranged by size: 3737 1079 971 351 278 89 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4696 4730 1903 Size: 34 2759 34 3678 Fragments arranged by size: 3678 2759 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4397 5607 4397 Size: 1210 5295 BclI T'GATC_A Cuts at: 1548 1548 Size: 6505 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 5116 136 Size: 107 122 71 631 605 343 3101 1525 Fragments arranged by size: 3101 1525 631 605 343 122 107 71 BglII A'GATC_T Cuts at: 3108 6500 3108 Size: 3392 3113 BlpI GC'TnA_GC Cuts at: 3922 3922 Size: 6505 Bme1580I G_kGCm'C Cuts at: 1790 3185 4566 5722 1790 Size: 1395 1381 1156 2573 Fragments arranged by size: 2573 1395 1381 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6505 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2419 3251 4236 Size: 416 53 83 399 24 1002 832 985 Cuts at: 4236 5194 442 Size: 958 1753 Fragments arranged by size: 1753 1002 985 958 832 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6505 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2365 3116 5165 1239 Size: 1126 751 2049 2579 Fragments arranged by size: 2579 2049 1126 751 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2126 4630 5495 5793 2126 Size: 2504 865 298 2838 Fragments arranged by size: 2838 2504 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5168 882 Size: 617 3669 2219 Fragments arranged by size: 3669 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4296 493 Size: 711 3092 2702 Fragments arranged by size: 3092 2702 711 BsaBI GATnn'nnATC Cuts at: 2849 6499 2849 Size: 3650 2855 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 3798 4312 4694 Size: 53 83 186 936 485 1780 514 382 Cuts at: 4694 5329 275 Size: 635 1451 Fragments arranged by size: 1780 1451 936 635 514 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4938 5336 5610 5757 6168 4938 Size: 398 274 147 411 5275 Fragments arranged by size: 5275 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2945 2948 2975 2978 1178 Size: 30 165 30 1542 3 27 3 4705 Fragments arranged by size: 4705 1542 165 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2953 3191 3536 1186 Size: 1767 238 345 4155 Fragments arranged by size: 4155 1767 345 238 BseYI C'CCAG_C Cuts at: 2083 2988 3066 3443 5708 6204 2083 Size: 905 78 377 2265 496 2384 Fragments arranged by size: 2384 2265 905 496 377 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5932 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3955 4716 5744 1540 Size: 275 186 1954 761 1028 2301 Fragments arranged by size: 2301 1954 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4566 4651 5722 4566 Size: 85 1071 5349 Fragments arranged by size: 5349 1071 85 BsmI GAATG_Cn' Cuts at: 4016 4109 4016 Size: 93 6412 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3185 3780 3824 4566 4651 5722 1391 Size: 399 1395 595 44 742 85 1071 2174 Fragments arranged by size: 2174 1395 1071 742 595 399 85 44 BspEI T'CCGG_A Cuts at: 6168 6168 Size: 6505 BspHI T'CATG_A Cuts at: 3114 4394 6124 3114 Size: 1280 1730 3495 Fragments arranged by size: 3495 1730 1280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3860 844 Size: 3016 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2012 4392 1394 Size: 429 189 2380 3507 Fragments arranged by size: 3507 2380 429 189 BsrDI GCAATG_nn' Cuts at: 65 1248 3045 3534 5000 5174 65 Size: 1183 1797 489 1466 174 1396 Fragments arranged by size: 1797 1466 1396 1183 489 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5149 5336 1700 Size: 110 3339 187 2869 Fragments arranged by size: 3339 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6505 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6505 BssSI C'ACGA_G Cuts at: 4565 5577 4565 Size: 1012 5493 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6505 BstBI TT'CG_AA Cuts at: 2857 5309 2857 Size: 2452 4053 BstEII G'GTnAC_C Cuts at: 1184 3757 1184 Size: 2573 3932 BstXI CCAn_nnnn'nTGG Cuts at: 3264 3443 3570 3264 Size: 179 127 6199 Fragments arranged by size: 6199 179 127 BstYI r'GATC_y Cuts at: 1972 3108 3835 4202 4587 4604 6056 6142 6154 Size: 1136 727 367 385 17 1452 86 12 Cuts at: 6154 6500 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1136 727 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2041 2058 2571 3771 3801 513 Size: 552 939 37 17 513 1200 30 3217 Fragments arranged by size: 3217 1200 939 552 513 37 30 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5766 Fragments arranged by size: 5766 683 56 BtsI GCAGTG_nn' Cuts at: 2619 4021 4815 4835 2619 Size: 1402 794 20 4289 Fragments arranged by size: 4289 1402 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6505 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6470 DraI TTT'AAA Cuts at: 2337 2881 3809 2337 Size: 544 928 5033 Fragments arranged by size: 5033 928 544 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5512 809 Size: 507 4196 1802 Fragments arranged by size: 4196 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2055 2103 Size: 54 1425 50 275 18 177 48 48 Cuts at: 2103 3603 3952 4841 6470 8 Size: 1500 349 889 1629 43 Fragments arranged by size: 1629 1500 1425 889 349 275 177 54 50 48 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3952 1537 Size: 275 2140 4090 Fragments arranged by size: 4090 2140 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3041 3257 3333 4435 1353 Size: 1688 216 76 1102 3423 Fragments arranged by size: 3423 1688 1102 216 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5092 5466 5612 387 Size: 1064 138 3503 374 146 1280 Fragments arranged by size: 3503 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2262 2365 2428 2484 3116 3314 3636 Size: 393 630 103 63 56 632 198 322 Cuts at: 3636 4568 5165 5952 6220 1239 Size: 932 597 787 268 1524 Fragments arranged by size: 1524 932 787 632 630 597 393 322 268 198 103 63 56 EcoNI CCTnn'n_nnAGG Cuts at: 2982 2982 Size: 6505 EcoO109I rG'GnC_Cy Cuts at: 3136 3323 3136 Size: 187 6318 EcoRI G'AATT_C Cuts at: 3814 4192 3814 Size: 378 6127 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2458 2678 2458 Size: 220 6285 FspI TGC'GCA Cuts at: 5011 5011 Size: 6505 HaeII r_GCGC'y Cuts at: 1678 2021 3032 3801 5652 1678 Size: 343 1011 769 1851 2531 Fragments arranged by size: 2531 1851 1011 769 343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3328 3360 5146 5178 5220 Size: 32 135 32 1756 32 1786 32 42 Cuts at: 5220 5252 1373 Size: 32 2626 Fragments arranged by size: 2626 1786 1756 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2491 3226 3849 4095 669 Size: 988 281 553 735 623 246 3079 Fragments arranged by size: 3079 988 735 623 553 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6505 HpaI GTT'AAC Cuts at: 2491 4095 2491 Size: 1604 4901 KasI G'GCGC_C Cuts at: 2017 3797 2017 Size: 1780 4725 KpnI G_GTAC'C Cuts at: 2424 3830 2424 Size: 1406 5099 MfeI C'AATT_G Cuts at: 2943 4104 2943 Size: 1161 5344 MluI A'CGCG_T Cuts at: 6381 6381 Size: 6505 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5619 5803 1305 Size: 4314 184 2007 Fragments arranged by size: 4314 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4683 Fragments arranged by size: 4683 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 2601 2807 3568 4463 4822 4981 Size: 648 1041 394 206 761 895 359 159 Cuts at: 4981 518 Size: 2042 Fragments arranged by size: 2042 1041 895 761 648 394 359 206 159 MspA1I CmG'CkG Cuts at: 1606 2043 2060 3455 3921 4598 5746 5991 1606 Size: 437 17 1395 466 677 1148 245 2120 Fragments arranged by size: 2120 1395 1148 677 466 437 245 17 NarI GG'CG_CC Cuts at: 2018 3798 2018 Size: 1780 4725 NcoI C'CATG_G Cuts at: 513 1065 2004 2571 513 Size: 552 939 567 4447 Fragments arranged by size: 4447 939 567 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6505 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6505 NotI GC'GGCC_GC Cuts at: 3952 3952 Size: 6505 NspI r_CATG'y Cuts at: 1132 1229 3863 5408 1132 Size: 97 2634 1545 2229 Fragments arranged by size: 2634 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2968 3781 1348 Size: 1620 813 4072 Fragments arranged by size: 4072 1620 813 PciI A'CATG_T Cuts at: 5404 5404 Size: 6505 PflMI CCAn_nnn'nTGG Cuts at: 1108 2134 2417 3139 3592 1108 Size: 1026 283 722 453 4021 Fragments arranged by size: 4021 1026 722 453 283 PfoI T'CCnGG_A Cuts at: 3591 3591 Size: 6505 PmeI GTTT'AAAC Cuts at: 3809 3809 Size: 6505 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4574 4606 6087 6119 4574 Size: 32 1481 32 4960 Fragments arranged by size: 4960 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3136 3323 3136 Size: 187 6318 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6505 PsiI TTA'TAA Cuts at: 4075 4075 Size: 6505 PstI C_TGCA'G Cuts at: 830 2097 3857 830 Size: 1267 1760 3478 Fragments arranged by size: 3478 1760 1267 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6505 PvuII CAG'CTG Cuts at: 3455 3455 Size: 6505 SacII CC_GC'GG Cuts at: 2044 2061 2044 Size: 17 6488 SalI G'TCGA_C Cuts at: 1936 3224 3847 1936 Size: 1288 623 4594 Fragments arranged by size: 4594 1288 623 SbfI CC_TGCA'GG Cuts at: 3857 3857 Size: 6505 ScaI AGT'ACT Cuts at: 4753 6465 4753 Size: 1712 4793 SfcI C'TryA_G Cuts at: 826 1045 2093 3853 4988 5669 5860 826 Size: 219 1048 1760 1135 681 191 1471 Fragments arranged by size: 1760 1471 1135 1048 681 219 191 SfoI GGC'GCC Cuts at: 2019 3799 2019 Size: 1780 4725 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6505 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6505 SmaI CCC'GGG Cuts at: 1872 3386 3832 1872 Size: 1514 446 4545 Fragments arranged by size: 4545 1514 446 SmlI C'TyrA_G Cuts at: 820 1017 1943 2141 4609 5510 5772 820 Size: 197 926 198 2468 901 262 1553 Fragments arranged by size: 2468 1553 926 901 262 198 197 SnaBI TAC'GTA Cuts at: 493 4296 493 Size: 3803 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6505 SphI G_CATG'C Cuts at: 1132 3863 1132 Size: 2731 3774 SrfI GCCC'GGGC Cuts at: 3386 3386 Size: 6505 SspI AAT'ATT Cuts at: 5 52 4429 5 Size: 47 4377 2081 Fragments arranged by size: 4377 2081 47 StuI AGG'CCT Cuts at: 1517 2661 1517 Size: 1144 5361 StyI C'CwwG_G Cuts at: 513 1065 2004 2048 2464 2571 2689 2928 3944 Size: 552 939 44 416 107 118 239 1016 Cuts at: 3944 513 Size: 3074 Fragments arranged by size: 3074 1016 939 552 416 239 118 107 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4532 4549 4702 4887 5332 325 Size: 1255 2952 17 153 185 445 1498 Fragments arranged by size: 2952 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2552 3000 4751 Size: 274 80 33 51 1443 575 448 1751 Cuts at: 4751 6463 96 Size: 1712 138 Fragments arranged by size: 1751 1712 1443 575 448 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2945 2977 3120 3152 6465 6497 2945 Size: 32 143 32 3313 32 2953 Fragments arranged by size: 3313 2953 143 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6505 XbaI T'CTAG_A Cuts at: 3841 3841 Size: 6505 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3769 2011 Size: 1758 4747 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6505 XmaI C'CCGG_G Cuts at: 1870 3384 3830 1870 Size: 1514 446 4545 Fragments arranged by size: 4545 1514 446 XmnI GAAnn'nnTTC Cuts at: 3482 4634 3482 Size: 1152 5353 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4313 276 Size: 53 83 186 936 2779 2468 Fragments arranged by size: 2779 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI