(Circular) (Six-base) MAPSORT of: pFN21AA1621.seq Check: 6918 from: 1 to: 7033 pFN21AA1621 7033 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3001 3504 3001 Size: 503 6530 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4843 278 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4068 4376 1937 Size: 2131 308 4594 Fragments arranged by size: 4594 2131 308 Acc65I G'GTAC_C Cuts at: 4354 4354 Size: 7033 AclI AA'CG_TT Cuts at: 1563 5160 5533 1563 Size: 3597 373 3063 Fragments arranged by size: 3597 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3174 4325 5096 6480 6748 1632 Size: 1542 1151 771 1384 268 1917 Fragments arranged by size: 1917 1542 1384 1151 771 268 AfeI AGC'GCT Cuts at: 2646 2646 Size: 7033 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6836 AflIII A'CryG_T Cuts at: 1203 5932 6909 1203 Size: 4729 977 1327 Fragments arranged by size: 4729 1327 977 AgeI A'CCGG_T Cuts at: 5864 5864 Size: 7033 AhdI GACnn_n'nnGTC Cuts at: 5762 5762 Size: 7033 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7033 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3572 3604 3572 Size: 32 7001 AlwNI CAG_nnn'CTG Cuts at: 3122 4776 6348 3122 Size: 1654 1572 3807 Fragments arranged by size: 3807 1654 1572 ApaI G_GGCC'C Cuts at: 4196 4196 Size: 7033 ApaLI G'TGCA_C Cuts at: 2995 5090 6246 2995 Size: 2095 1156 3782 Fragments arranged by size: 3782 2095 1156 ApoI r'AATT_y Cuts at: 1493 2555 4342 4568 4720 6829 1493 Size: 1062 1787 226 152 2109 1697 Fragments arranged by size: 2109 1787 1697 1062 226 152 AseI AT'TA_AT Cuts at: 160 1032 5587 160 Size: 872 4555 1606 Fragments arranged by size: 4555 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 AvaI C'yCGr_G Cuts at: 1870 1943 3735 3790 3839 4358 1870 Size: 73 1792 55 49 519 4545 Fragments arranged by size: 4545 1792 519 73 55 49 BamHI G'GATC_C Cuts at: 4363 4730 4363 Size: 367 6666 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 3730 4226 4354 5809 Size: 325 217 627 297 1646 496 128 1455 Cuts at: 5809 618 Size: 1842 Fragments arranged by size: 1842 1646 1455 627 496 325 297 217 128 BanII G_rGCy'C Cuts at: 1391 3840 4196 4352 1391 Size: 2449 356 156 4072 Fragments arranged by size: 4072 2449 356 156 BbeI G_GCGC'C Cuts at: 2088 2088 Size: 7033 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3149 3171 928 Size: 971 1250 22 4790 Fragments arranged by size: 4790 1250 971 22 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5224 5258 1903 Size: 34 3287 34 3678 Fragments arranged by size: 3678 3287 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4925 6135 4925 Size: 1210 5823 BclI T'GATC_A Cuts at: 1548 1548 Size: 7033 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5644 136 Size: 107 122 71 631 605 3972 1525 Fragments arranged by size: 3972 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7028 7028 Size: 7033 BlpI GC'TnA_GC Cuts at: 2066 3893 4450 2066 Size: 1827 557 4649 Fragments arranged by size: 4649 1827 557 Bme1580I G_kGCm'C Cuts at: 1790 2999 3735 4196 5094 6250 1790 Size: 1209 736 461 898 1156 2573 Fragments arranged by size: 2573 1209 1156 898 736 461 BmgBI CAC'GTC Cuts at: 1318 3022 1318 Size: 1704 5329 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2256 4764 5722 Size: 416 53 83 399 24 839 2508 958 Cuts at: 5722 442 Size: 1753 Fragments arranged by size: 2508 1753 958 839 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7033 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2146 2146 Size: 7033 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5693 1239 Size: 4454 2579 Bpu10I CC'TnA_GC Cuts at: 2580 2682 2580 Size: 102 6931 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2219 3809 5158 6023 6321 2219 Size: 1590 1349 865 298 2931 Fragments arranged by size: 2931 1590 1349 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3520 5696 882 Size: 617 2021 2176 2219 Fragments arranged by size: 2219 2176 2021 617 BsaAI yAC'GTr Cuts at: 493 1204 3982 4824 493 Size: 711 2778 842 2702 Fragments arranged by size: 2778 2702 842 711 BsaBI GATnn'nnATC Cuts at: 7027 7027 Size: 7033 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 3663 4207 4840 Size: 53 83 186 936 552 1578 544 633 Cuts at: 4840 5222 5857 275 Size: 382 635 1451 Fragments arranged by size: 1578 1451 936 635 633 552 544 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2431 3128 5466 5864 6138 6285 6696 2431 Size: 697 2338 398 274 147 411 2768 Fragments arranged by size: 2768 2338 697 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3572 3599 3602 3629 3986 Size: 30 165 30 2169 27 3 27 357 Cuts at: 3986 4016 1178 Size: 30 4195 Fragments arranged by size: 4195 2169 357 165 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2627 2630 4165 1186 Size: 1111 330 3 1535 4054 Fragments arranged by size: 4054 1535 1111 330 3 BseYI C'CCAG_C Cuts at: 4005 6236 6732 4005 Size: 2231 496 4306 Fragments arranged by size: 4306 2231 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2771 3039 3341 3392 3703 3962 4165 Size: 573 1035 268 302 51 311 259 203 Cuts at: 4165 1163 Size: 4031 Fragments arranged by size: 4031 1035 573 311 302 268 259 203 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3744 4483 5244 6272 1540 Size: 275 186 1743 739 761 1028 2301 Fragments arranged by size: 2301 1743 1028 761 739 275 186 BsiHKAI G_wGCw'C Cuts at: 2999 3229 3309 3962 3987 4122 5094 5179 6250 Size: 230 80 653 25 135 972 85 1071 Cuts at: 6250 2999 Size: 3782 Fragments arranged by size: 3782 1071 972 653 230 135 85 80 25 BsmI GAATG_Cn' Cuts at: 4544 4637 4544 Size: 93 6940 BsmBI CGTCTCn'nnnn_ Cuts at: 2800 2800 Size: 7033 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2999 3229 3309 3735 3840 3962 3987 Size: 399 1209 230 80 426 105 122 25 Cuts at: 3987 4122 4196 4352 5094 5179 6250 1391 Size: 135 74 156 742 85 1071 2174 Fragments arranged by size: 2174 1209 1071 742 426 399 230 156 135 122 105 85 80 74 25 BspEI T'CCGG_A Cuts at: 3128 6696 3128 Size: 3568 3465 BspHI T'CATG_A Cuts at: 4922 6652 4922 Size: 1730 5303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3001 3504 4388 844 Size: 2157 503 884 3489 Fragments arranged by size: 3489 2157 884 503 BsrBI CCG'CTC Cuts at: 1394 1823 4021 4920 1394 Size: 429 2198 899 3507 Fragments arranged by size: 3507 2198 899 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5528 5702 65 Size: 1183 4280 174 1396 Fragments arranged by size: 4280 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5677 5864 1700 Size: 110 3867 187 2869 Fragments arranged by size: 3867 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7033 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7033 BssSI C'ACGA_G Cuts at: 5093 6105 5093 Size: 1012 6021 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7033 BstBI TT'CG_AA Cuts at: 2931 5837 2931 Size: 2906 4127 BstEII G'GTnAC_C Cuts at: 1184 3043 3460 3760 1184 Size: 1859 417 300 4457 Fragments arranged by size: 4457 1859 417 300 BstXI CCAn_nnnn'nTGG Cuts at: 3772 3772 Size: 7033 BstYI r'GATC_y Cuts at: 1972 3118 4363 4730 5115 5132 6584 6670 6682 Size: 1146 1245 367 385 17 1452 86 12 Cuts at: 6682 7028 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1245 1146 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3551 4188 3551 Size: 637 6396 BtgI C'CryG_G Cuts at: 513 1065 2004 3610 3831 513 Size: 552 939 1606 221 3715 Fragments arranged by size: 3715 1606 939 552 221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6294 Fragments arranged by size: 6294 683 56 BtsI GCAGTG_nn' Cuts at: 4549 5343 5363 4549 Size: 794 20 6219 Fragments arranged by size: 6219 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7033 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6998 DraI TTT'AAA Cuts at: 4337 4337 Size: 7033 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2179 6040 809 Size: 507 863 3861 1802 Fragments arranged by size: 3861 1802 863 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3535 3741 3963 Size: 54 1425 50 275 18 1705 206 222 Cuts at: 3963 4039 4480 5369 6998 8 Size: 76 441 889 1629 43 Fragments arranged by size: 1705 1629 1425 889 441 275 222 206 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3741 4480 1537 Size: 275 1929 739 4090 Fragments arranged by size: 4090 1929 739 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3006 4109 4963 1353 Size: 1653 1103 854 3423 Fragments arranged by size: 3423 1653 1103 854 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2788 2832 5620 5994 6140 387 Size: 1064 138 1199 44 2788 374 146 1280 Fragments arranged by size: 2788 1280 1199 1064 374 146 138 44 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3174 4325 5096 5693 6480 6748 1239 Size: 393 1542 1151 771 597 787 268 1524 Fragments arranged by size: 1542 1524 1151 787 771 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2895 2895 Size: 7033 EcoO109I rG'GnC_Cy Cuts at: 2097 3487 3571 4192 4193 4233 2097 Size: 1390 84 621 1 40 4897 Fragments arranged by size: 4897 1390 621 84 40 1 EcoRI G'AATT_C Cuts at: 2555 4342 4720 2555 Size: 1787 378 4868 Fragments arranged by size: 4868 1787 378 EcoRV GAT'ATC Cuts at: 3327 3327 Size: 7033 FspI TGC'GCA Cuts at: 2957 3878 5539 2957 Size: 921 1661 4451 Fragments arranged by size: 4451 1661 921 FspAI rTGC'GCAy Cuts at: 2957 3878 2957 Size: 921 6112 HaeII r_GCGC'y Cuts at: 1678 2088 2648 3429 3603 3641 6180 1678 Size: 410 560 781 174 38 2539 2531 Fragments arranged by size: 2539 2531 781 560 410 174 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2011 2043 2081 2113 2642 Size: 32 135 32 439 32 38 32 529 Cuts at: 2642 2674 3110 3142 3425 3457 3599 3631 3773 Size: 32 436 32 283 32 142 32 142 Cuts at: 3773 3805 3986 4018 5674 5706 5748 5780 1373 Size: 32 181 32 1656 32 42 32 2626 Fragments arranged by size: 2626 1656 529 439 436 283 181 142 142 135 42 38 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2172 4377 4623 669 Size: 988 281 234 2205 246 3079 Fragments arranged by size: 3079 2205 988 281 246 234 HindIII A'AGCT_T Cuts at: 748 748 Size: 7033 HpaI GTT'AAC Cuts at: 4623 4623 Size: 7033 KasI G'GCGC_C Cuts at: 2084 2084 Size: 7033 KpnI G_GTAC'C Cuts at: 4358 4358 Size: 7033 MfeI C'AATT_G Cuts at: 4632 4632 Size: 7033 MluI A'CGCG_T Cuts at: 6909 6909 Size: 7033 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2571 6147 6331 1305 Size: 1266 3576 184 2007 Fragments arranged by size: 3576 2007 1266 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5211 Fragments arranged by size: 5211 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3278 3317 4991 5350 5509 518 Size: 648 2112 39 1674 359 159 2042 Fragments arranged by size: 2112 2042 1674 648 359 159 39 MspA1I CmG'CkG Cuts at: 1606 3612 3697 3820 4176 4449 5126 6274 6519 Size: 2006 85 123 356 273 677 1148 245 Cuts at: 6519 1606 Size: 2120 Fragments arranged by size: 2120 2006 1148 677 356 273 245 123 85 NarI GG'CG_CC Cuts at: 2085 2085 Size: 7033 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5542 Fragments arranged by size: 5542 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7033 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7033 NotI GC'GGCC_GC Cuts at: 4480 4480 Size: 7033 NspI r_CATG'y Cuts at: 1132 1229 4391 5936 1132 Size: 97 3162 1545 2229 Fragments arranged by size: 3162 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2209 1348 Size: 861 6172 PciI A'CATG_T Cuts at: 5932 5932 Size: 7033 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7033 PfoI T'CCnGG_A Cuts at: 3159 3159 Size: 7033 PmeI GTTT'AAAC Cuts at: 4337 4337 Size: 7033 PmlI CAC'GTG Cuts at: 3982 3982 Size: 7033 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3572 3604 4283 4315 5102 5134 6615 6647 3572 Size: 32 679 32 787 32 1481 32 3958 Fragments arranged by size: 3958 1481 787 679 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2097 3487 4233 2097 Size: 1390 746 4897 Fragments arranged by size: 4897 1390 746 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7033 PsiI TTA'TAA Cuts at: 4603 4603 Size: 7033 PspOMI G'GGCC_C Cuts at: 4192 4192 Size: 7033 PstI C_TGCA'G Cuts at: 830 3012 3702 4385 830 Size: 2182 690 683 3478 Fragments arranged by size: 3478 2182 690 683 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7033 PvuII CAG'CTG Cuts at: 3820 3820 Size: 7033 SacII CC_GC'GG Cuts at: 3613 3613 Size: 7033 SalI G'TCGA_C Cuts at: 1936 4375 1936 Size: 2439 4594 SanDI GG'GwC_CC Cuts at: 4233 4233 Size: 7033 SapI GCTCTTCn'nnn_ Cuts at: 4109 4109 Size: 7033 SbfI CC_TGCA'GG Cuts at: 3012 4385 3012 Size: 1373 5660 ScaI AGT'ACT Cuts at: 5281 6993 5281 Size: 1712 5321 SexAI A'CCwGG_T Cuts at: 3756 4071 3756 Size: 315 6718 SfcI C'TryA_G Cuts at: 826 1045 3008 3698 4381 5516 6197 6388 826 Size: 219 1963 690 683 1135 681 191 1471 Fragments arranged by size: 1963 1471 1135 690 683 681 219 191 SfoI GGC'GCC Cuts at: 2086 2086 Size: 7033 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7033 SmaI CCC'GGG Cuts at: 1872 3737 3841 4360 1872 Size: 1865 104 519 4545 Fragments arranged by size: 4545 1865 519 104 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 3824 5137 6038 6300 820 Size: 197 926 291 1590 1313 901 262 1553 Fragments arranged by size: 1590 1553 1313 926 901 291 262 197 SnaBI TAC'GTA Cuts at: 493 4824 493 Size: 4331 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7033 SphI G_CATG'C Cuts at: 1132 4391 1132 Size: 3259 3774 SrfI GCCC'GGGC Cuts at: 3841 3841 Size: 7033 SspI AAT'ATT Cuts at: 5 52 2352 4957 5 Size: 47 2300 2605 2081 Fragments arranged by size: 2605 2300 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7033 StyI C'CwwG_G Cuts at: 513 1065 2004 3210 3290 3765 4472 513 Size: 552 939 1206 80 475 707 3074 Fragments arranged by size: 3074 1206 939 707 552 475 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3374 5060 5077 5230 5415 5860 325 Size: 1255 1794 1686 17 153 185 445 1498 Fragments arranged by size: 1794 1686 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5279 6991 96 Size: 274 80 33 51 1443 3302 1712 138 Fragments arranged by size: 3302 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6993 7025 6993 Size: 32 7001 Tth111I GACn'n_nGTC Cuts at: 1316 3049 4237 1316 Size: 1733 1188 4112 Fragments arranged by size: 4112 1733 1188 XbaI T'CTAG_A Cuts at: 4369 4369 Size: 7033 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3919 3919 Size: 7033 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7033 XmaI C'CCGG_G Cuts at: 1870 3735 3839 4358 1870 Size: 1865 104 519 4545 Fragments arranged by size: 4545 1865 519 104 XmnI GAAnn'nnTTC Cuts at: 2352 2418 3168 5162 2352 Size: 66 750 1994 4223 Fragments arranged by size: 4223 1994 750 66 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4841 276 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoICRI FalI FseI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SfiI SwaI