(Circular) (Six-base) MAPSORT of: pFN21AA1597.seq Check: 902 from: 1 to: 7390 pFN21AA1597 7390 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5200 278 Size: 53 83 186 936 3664 2468 Fragments arranged by size: 3664 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4733 1937 Size: 2796 4594 Acc65I G'GTAC_C Cuts at: 4143 4711 4143 Size: 568 6822 AclI AA'CG_TT Cuts at: 1563 5517 5890 1563 Size: 3954 373 3063 Fragments arranged by size: 3954 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 2117 3588 3833 4172 4470 4572 4584 Size: 422 63 1471 245 339 298 102 12 Cuts at: 4584 5453 6837 7105 1632 Size: 869 1384 268 1917 Fragments arranged by size: 1917 1471 1384 869 422 339 298 268 245 102 63 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7193 AflIII A'CryG_T Cuts at: 1203 6289 7266 1203 Size: 5086 977 1327 Fragments arranged by size: 5086 1327 977 AgeI A'CCGG_T Cuts at: 6221 6221 Size: 7390 AhdI GACnn_n'nnGTC Cuts at: 6119 6119 Size: 7390 AleI CACnn'nnGTG Cuts at: 1166 4427 1166 Size: 3261 4129 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2040 1184 Size: 856 6534 AlwNI CAG_nnn'CTG Cuts at: 2095 4238 5133 6705 2095 Size: 2143 895 1572 2780 Fragments arranged by size: 2780 2143 1572 895 ApaLI G'TGCA_C Cuts at: 4268 5447 6603 4268 Size: 1179 1156 5055 Fragments arranged by size: 5055 1179 1156 ApoI r'AATT_y Cuts at: 1493 2886 3023 3265 3621 4324 4699 4925 5077 Size: 1393 137 242 356 703 375 226 152 Cuts at: 5077 7186 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1393 703 375 356 242 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 3210 5944 160 Size: 872 2178 2734 1606 Fragments arranged by size: 2734 2178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7390 AvaI C'yCGr_G Cuts at: 1870 1943 2344 4715 1870 Size: 73 401 2371 4545 Fragments arranged by size: 4545 2371 401 73 AvrII C'CTAG_G Cuts at: 4075 4075 Size: 7390 BamHI G'GATC_C Cuts at: 2772 4720 5087 2772 Size: 1948 367 5075 Fragments arranged by size: 5075 1948 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3385 4143 4711 6166 618 Size: 325 217 627 1598 758 568 1455 1842 Fragments arranged by size: 1842 1598 1455 758 627 568 325 217 BanII G_rGCy'C Cuts at: 1391 2764 4709 1391 Size: 1373 1945 4072 Fragments arranged by size: 4072 1945 1373 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2919 3504 4166 4385 4517 928 Size: 971 1020 585 662 219 132 3801 Fragments arranged by size: 3801 1020 971 662 585 219 132 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5581 5615 1903 Size: 34 3644 34 3678 Fragments arranged by size: 3678 3644 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4045 5282 6492 4045 Size: 1237 1210 4943 Fragments arranged by size: 4943 1237 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7390 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6001 136 Size: 107 122 71 631 605 4329 1525 Fragments arranged by size: 4329 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4453 7385 4453 Size: 2932 4458 BlpI GC'TnA_GC Cuts at: 2214 4807 2214 Size: 2593 4797 Bme1580I G_kGCm'C Cuts at: 1790 4272 5451 6607 1790 Size: 2482 1179 1156 2573 Fragments arranged by size: 2573 2482 1179 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7390 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3429 3832 4120 Size: 416 53 83 399 24 2012 403 288 Cuts at: 4120 5121 6079 442 Size: 1001 958 1753 Fragments arranged by size: 2012 1753 1001 958 416 403 399 288 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7390 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2778 2792 2778 Size: 14 7376 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2274 2406 2773 2957 4199 4281 6050 1239 Size: 1035 132 367 184 1242 82 1769 2579 Fragments arranged by size: 2579 1769 1242 1035 367 184 132 82 Bpu10I CC'TnA_GC Cuts at: 3102 3372 4307 3102 Size: 270 935 6185 Fragments arranged by size: 6185 935 270 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2762 3133 5515 6380 6678 2762 Size: 371 2382 865 298 3474 Fragments arranged by size: 3474 2382 865 371 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3399 6053 882 Size: 617 1900 2654 2219 Fragments arranged by size: 2654 2219 1900 617 BsaAI yAC'GTr Cuts at: 493 1204 5181 493 Size: 711 3977 2702 Fragments arranged by size: 3977 2702 711 BsaBI GATnn'nnATC Cuts at: 7384 7384 Size: 7390 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5197 5579 6214 275 Size: 53 83 186 936 3664 382 635 1451 Fragments arranged by size: 3664 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5823 6221 6495 6642 7053 5823 Size: 398 274 147 411 6160 Fragments arranged by size: 6160 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4197 4227 1178 Size: 30 165 30 2794 30 4341 Fragments arranged by size: 4341 2794 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7390 BseYI C'CCAG_C Cuts at: 2253 6593 7089 2253 Size: 4340 496 2554 Fragments arranged by size: 4340 2554 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6817 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4840 5601 6629 1540 Size: 275 186 2839 761 1028 2301 Fragments arranged by size: 2839 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4272 5451 5536 6607 4272 Size: 1179 85 1071 5055 Fragments arranged by size: 5055 1179 1071 85 BsmI GAATG_Cn' Cuts at: 2902 3220 3614 4292 4668 4901 4994 2902 Size: 318 394 678 376 233 93 5298 Fragments arranged by size: 5298 678 394 376 318 233 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2764 4272 4709 5451 5536 6607 1391 Size: 399 974 1508 437 742 85 1071 2174 Fragments arranged by size: 2174 1508 1071 974 742 437 399 85 BspEI T'CCGG_A Cuts at: 7053 7053 Size: 7390 BspHI T'CATG_A Cuts at: 5279 7009 5279 Size: 1730 5660 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4745 844 Size: 3901 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5277 1394 Size: 429 3454 3507 Fragments arranged by size: 3507 3454 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5885 6059 65 Size: 1183 4637 174 1396 Fragments arranged by size: 4637 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6034 6221 1700 Size: 110 4224 187 2869 Fragments arranged by size: 4224 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7390 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7390 BssSI C'ACGA_G Cuts at: 5450 6462 5450 Size: 1012 6378 BstAPI GCAn_nnn'nTGC Cuts at: 794 2620 4172 794 Size: 1826 1552 4012 Fragments arranged by size: 4012 1826 1552 BstBI TT'CG_AA Cuts at: 6194 6194 Size: 7390 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7390 BstYI r'GATC_y Cuts at: 1972 2772 4453 4720 5087 5472 5489 6941 7027 Size: 800 1681 267 367 385 17 1452 86 Cuts at: 7027 7039 7385 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1681 1452 800 385 367 346 267 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2158 3004 513 Size: 552 1093 846 4899 Fragments arranged by size: 4899 1093 846 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6651 Fragments arranged by size: 6651 683 56 BtsI GCAGTG_nn' Cuts at: 2973 3733 4301 4649 4906 5700 5720 2973 Size: 760 568 348 257 794 20 4643 Fragments arranged by size: 4643 794 760 568 348 257 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7390 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7355 DraI TTT'AAA Cuts at: 2700 4694 2700 Size: 1994 5396 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6397 809 Size: 507 5081 1802 Fragments arranged by size: 5081 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2110 4837 5726 Size: 54 1425 50 275 18 280 2727 889 Cuts at: 5726 7355 8 Size: 1629 43 Fragments arranged by size: 2727 1629 1425 889 280 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4837 1537 Size: 275 3025 4090 Fragments arranged by size: 4090 3025 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2030 2043 2189 2307 2316 2423 2926 3078 Size: 677 13 146 118 9 107 503 152 Cuts at: 3078 5320 1353 Size: 2242 3423 Fragments arranged by size: 3423 2242 677 503 152 146 118 107 13 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5977 6351 6497 387 Size: 1064 138 4388 374 146 1280 Fragments arranged by size: 4388 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2117 2274 2406 2773 2957 3588 Size: 393 422 63 157 132 367 184 631 Cuts at: 3588 3833 4172 4199 4281 4470 4572 4584 5453 Size: 245 339 27 82 189 102 12 869 Cuts at: 5453 6050 6837 7105 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 869 787 631 597 422 393 367 339 268 245 189 184 157 132 102 82 63 27 12 EcoNI CCTnn'n_nnAGG Cuts at: 4079 4079 Size: 7390 EcoO109I rG'GnC_Cy Cuts at: 2199 2640 2199 Size: 441 6949 EcoRI G'AATT_C Cuts at: 3265 4699 5077 3265 Size: 1434 378 5578 Fragments arranged by size: 5578 1434 378 EcoRV GAT'ATC Cuts at: 2170 2170 Size: 7390 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3976 3976 Size: 7390 FspI TGC'GCA Cuts at: 5896 5896 Size: 7390 HaeII r_GCGC'y Cuts at: 1678 6537 1678 Size: 4859 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2422 2454 2534 2566 2702 Size: 32 135 32 850 32 80 32 136 Cuts at: 2702 2734 3284 3316 3338 3370 3491 3523 6031 Size: 32 550 32 22 32 121 32 2508 Cuts at: 6031 6063 6105 6137 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2508 850 550 136 135 121 80 42 32 32 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 2269 2571 4734 4980 669 Size: 988 281 331 302 2163 246 3079 Fragments arranged by size: 3079 2163 988 331 302 281 246 HindIII A'AGCT_T Cuts at: 748 4247 748 Size: 3499 3891 HpaI GTT'AAC Cuts at: 4980 4980 Size: 7390 KpnI G_GTAC'C Cuts at: 4147 4715 4147 Size: 568 6822 MfeI C'AATT_G Cuts at: 3792 4133 4989 3792 Size: 341 856 6193 Fragments arranged by size: 6193 856 341 MluI A'CGCG_T Cuts at: 7266 7266 Size: 7390 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6504 6688 1305 Size: 5199 184 2007 Fragments arranged by size: 5199 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2112 10 Size: 54 1425 343 280 5288 Fragments arranged by size: 5288 1425 343 280 54 MslI CAynn'nnrTG Cuts at: 518 1166 4277 4427 5348 5707 5866 518 Size: 648 3111 150 921 359 159 2042 Fragments arranged by size: 3111 2042 921 648 359 159 150 MspA1I CmG'CkG Cuts at: 1606 2020 2095 2213 3860 4806 5483 6631 6876 Size: 414 75 118 1647 946 677 1148 245 Cuts at: 6876 1606 Size: 2120 Fragments arranged by size: 2120 1647 1148 946 677 414 245 118 75 NcoI C'CATG_G Cuts at: 513 1065 2158 3004 513 Size: 552 1093 846 4899 Fragments arranged by size: 4899 1093 846 552 NdeI CA'TA_TG Cuts at: 387 3157 3889 387 Size: 2770 732 3888 Fragments arranged by size: 3888 2770 732 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7390 NotI GC'GGCC_GC Cuts at: 4837 4837 Size: 7390 NspI r_CATG'y Cuts at: 1132 1229 2954 3827 4748 6293 1132 Size: 97 1725 873 921 1545 2229 Fragments arranged by size: 2229 1725 1545 921 873 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7390 PciI A'CATG_T Cuts at: 6289 6289 Size: 7390 PflMI CCAn_nnn'nTGG Cuts at: 1108 3212 4238 4426 4516 4635 1108 Size: 2104 1026 188 90 119 3863 Fragments arranged by size: 3863 2104 1026 188 119 90 PmeI GTTT'AAAC Cuts at: 4694 4694 Size: 7390 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5459 5491 6972 7004 5459 Size: 32 1481 32 5845 Fragments arranged by size: 5845 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2640 2640 Size: 7390 PshAI GACnn'nnGTC Cuts at: 1613 3253 1613 Size: 1640 5750 PsiI TTA'TAA Cuts at: 2826 4960 2826 Size: 2134 5256 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4424 4456 4424 Size: 32 7358 PstI C_TGCA'G Cuts at: 830 2968 4742 830 Size: 2138 1774 3478 Fragments arranged by size: 3478 2138 1774 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7390 PvuII CAG'CTG Cuts at: 2095 2213 2095 Size: 118 7272 SalI G'TCGA_C Cuts at: 1936 4732 1936 Size: 2796 4594 SanDI GG'GwC_CC Cuts at: 2640 2640 Size: 7390 SapI GCTCTTCn'nnn_ Cuts at: 2307 2307 Size: 7390 SbfI CC_TGCA'GG Cuts at: 4742 4742 Size: 7390 ScaI AGT'ACT Cuts at: 5638 7350 5638 Size: 1712 5678 SfcI C'TryA_G Cuts at: 826 1045 2964 4389 4738 5873 6554 6745 826 Size: 219 1919 1425 349 1135 681 191 1471 Fragments arranged by size: 1919 1471 1425 1135 681 349 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7390 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7390 SmaI CCC'GGG Cuts at: 1872 4717 1872 Size: 2845 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2777 3148 5494 6395 6657 820 Size: 197 926 834 371 2346 901 262 1553 Fragments arranged by size: 2346 1553 926 901 834 371 262 197 SnaBI TAC'GTA Cuts at: 493 5181 493 Size: 4688 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7390 SphI G_CATG'C Cuts at: 1132 4748 1132 Size: 3616 3774 SspI AAT'ATT Cuts at: 5 52 3781 5314 5 Size: 47 3729 1533 2081 Fragments arranged by size: 3729 2081 1533 47 StuI AGG'CCT Cuts at: 1517 2628 3899 4447 1517 Size: 1111 1271 548 4460 Fragments arranged by size: 4460 1271 1111 548 StyI C'CwwG_G Cuts at: 513 1065 2130 2158 3004 4075 4829 513 Size: 552 1065 28 846 1071 754 3074 Fragments arranged by size: 3074 1071 1065 846 754 552 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2245 4267 5417 5434 5587 5772 6217 Size: 1255 665 2022 1150 17 153 185 445 Cuts at: 6217 325 Size: 1498 Fragments arranged by size: 2022 1498 1255 1150 665 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5636 7348 96 Size: 274 80 33 51 1443 3659 1712 138 Fragments arranged by size: 3659 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7350 7382 7350 Size: 32 7358 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7390 XbaI T'CTAG_A Cuts at: 3401 3493 4726 3401 Size: 92 1233 6065 Fragments arranged by size: 6065 1233 92 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4513 4513 Size: 7390 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7390 XmaI C'CCGG_G Cuts at: 1870 4715 1870 Size: 2845 4545 XmnI GAAnn'nnTTC Cuts at: 2817 3087 3124 5519 2817 Size: 270 37 2395 4688 Fragments arranged by size: 4688 2395 270 37 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5198 276 Size: 53 83 186 936 3664 2468 Fragments arranged by size: 3664 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI