Restriction map for
pFN21AA1590
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# NO CUT ENZYMES ( 96 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI Aor51HI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BciVI BfrI
BglI BlnI BsaAI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspEI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstEII
BstPI BstXI BstZ17I Cfr10I CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FseI
FspI HpaI KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NcoI NgoMIV NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 652 GACGTC GACGT^C
AccII 2110 CGCG CG^CG
AflIII 468 ACGTGT A^C[GA][CT]GT
Alw44I 3887 GTGCAC G^TGCAC
ApaI 3020 GGGCCC GGGCC^C
ApaLI 3887 GTGCAC G^TGCAC
AseI 1090 ATTAAT AT^TAAT
AsnI 1090 ATTAAT AT^TAAT
BanI 3469 GGCACC G^G[CT][GA]CC
BbvCI 2049 GCTGAGG CCTCAGC(-5/-2)
BcgI 248 GCACAGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3902 TGATCA T^GATCA
BlpI 3163 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3163 GCTGAGC GC^T[ACGT]AGC
BsmBI 1445 GAGACG CGTCTC(1/5)
BspDI 1375 ATCGAT AT^CGAT
BspHI 989 TCATGA T^CATGA
BstAPI 2271 GCAGGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2110 CGCG CG^CG
CelII 3163 GCTGAGC GC^T[ACGT]AGC
ClaI 1375 ATCGAT AT^CGAT
DrdI 383 GACTCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1457 CCACGG C^C[GA][CT]GG
EcoNI 3177 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3902 TGATCA T^GATCA
HaeII 3009 GGCGCT [GA]GCGC^[CT]
MvnI 2110 CGCG CG^CG
NdeI 299 CATATG CA^TATG
NheI 3151 GCTAGC G^CTAGC
PshBI 1090 ATTAAT AT^TAAT
RcaI 989 TCATGA T^CATGA
SpeI 3975 ACTAGT A^CTAGT
XbaI 2614 TCTAGA T^CTAGA
XcmI 2225 CCAAGCTTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AcyI 652 GACGTC G[GA]^CG[CT]C
3383 GACGCC
AocI 1317 CCTCAGG CC^T[ACGT]AGG
4105 CCTGAGG
Asp700I 1216 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2383 GAAGGCATTC
BalI 3026 TGGCCA TGG^CCA
3777 TGGCCA
BanII 3020 GGGCCC G[GA]GC[CT]^C
3142 GGGCTC
BbsI 3835 GAAGAC GAAGAC(2/6)
3979 GTCTTC
BglII 477 AGATCT A^GATCT
3576 AGATCT
BpuAI 3835 GAAGAC GAAGAC(2/6)
3979 GTCTTC
BsaBI 1368 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3394 GATTTGCATC
BsaHI 652 GACGTC G[GA]^CG[CT]C
3383 GACGCC
BsaWI 623 ACCGGA [AT]^CCGG[AT]
784 ACCGGA
BsgI 773 GTGCAG GTGCAG(16/14)
1939 GTGCAG
BsmI 2387 GCATTC GAATGC(1/-1)
3857 GCATTC
BsrBI 1891 GAGCGG CCGCTC(-3/-3)
3016 GAGCGG
BssSI 2281 CTCGTG CACGAG(-5/-1)
2420 CTCGTG
BstBI 431 TTCGAA TT^CGAA
2476 TTCGAA
Bsu36I 1317 CCTCAGG CC^T[ACGT]AGG
4105 CCTGAGG
DraI 1416 TTTAAA TTT^AAA
2589 TTTAAA
Eco81I 1317 CCTCAGG CC^T[ACGT]AGG
4105 CCTGAGG
HgaI 3383 GACGC GACGC(5/10)
2111 GCGTC
Hin1I 652 GACGTC G[GA]^CG[CT]C
3383 GACGCC
HindIII 422 AAGCTT A^AGCTT
2227 AAGCTT
MamI 1368 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3394 GATTTGCATC
MluNI 3026 TGGCCA TGG^CCA
3777 TGGCCA
MscI 3026 TGGCCA TGG^CCA
3777 TGGCCA
NspV 431 TTCGAA TT^CGAA
2476 TTCGAA
PflMI 407 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1256 CCAAGGAATGG
PvuII 2893 CAGCTG CAG^CTG
3217 CAGCTG
SapI 2296 GAAGAGC GCTCTTC(1/4)
3088 GAAGAGC
ScaI 1039 AGTACT AGT^ACT
1393 AGTACT
SexAI 2525 ACCTGGT A^CC[AT]GGT
3326 ACCTGGT
SfuI 431 TTCGAA TT^CGAA
2476 TTCGAA
SspI 820 AATATT AAT^ATT
1291 AATATT
Van91I 407 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1256 CCAAGGAATGG
XmnI 1216 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2383 GAAGGCATTC
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII