Restriction map for
pFN21AA1590
(Linear) (Six-base) MAPSORT of: KIAA1590.seq Check: 4332 from: 1 to: 4176
KIAA1590.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1017 4176
Size: 1017 3159
AatII G_ACGT'C
Cuts at: 0 656 4176
Size: 656 3520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 458 1207 1698 3373 3986 4021 4176
Size: 372 86 749 491 1675 613 35 155
Fragments arranged by size:
1675 749 613 491 372 155 86 35
AflIII A'CryG_T
Cuts at: 0 468 4176
Size: 468 3708
AleI CACnn'nnGTG
Cuts at: 0 2659 3754 4176
Size: 2659 1095 422
Fragments arranged by size:
2659 1095 422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3122 4176
Size: 3122 1054
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 52 84 2561 2593 4176
Size: 52 32 2477 32 1583
Fragments arranged by size:
2477 1583 52 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 640 2334 3219 3535 3741 4176
Size: 640 1694 885 316 206 435
Fragments arranged by size:
1694 885 640 435 316 206
ApaI G_GGCC'C
Cuts at: 0 3024 4176
Size: 3024 1152
ApaLI G'TGCA_C
Cuts at: 0 3887 4176
Size: 3887 289
ApoI r'AATT_y
Cuts at: 0 342 455 703 1789 2032 2479 2786 2920
Size: 342 113 248 1086 243 447 307 134
Cuts at: 2920 3655 3925 4176
Size: 735 270 251
Fragments arranged by size:
1086 735 447 342 307 270 251 248
243 134 113
AseI AT'TA_AT
Cuts at: 0 1091 4176
Size: 1091 3085
BanI G'GyrC_C
Cuts at: 0 3469 4176
Size: 3469 707
BanII G_rGCy'C
Cuts at: 0 3024 3146 4176
Size: 3024 122 1030
Fragments arranged by size:
3024 1030 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 3842 3972 4176
Size: 3842 130 204
Fragments arranged by size:
3842 204 130
BbvCI CC'TCA_GC
Cuts at: 0 2050 4176
Size: 2050 2126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 237 271 4176
Size: 237 34 3905
Fragments arranged by size:
3905 237 34
BclI T'GATC_A
Cuts at: 0 3902 4176
Size: 3902 274
BglII A'GATC_T
Cuts at: 0 477 3576 4176
Size: 477 3099 600
Fragments arranged by size:
3099 600 477
BlpI GC'TnA_GC
Cuts at: 0 3164 4176
Size: 3164 1012
Bme1580I G_kGCm'C
Cuts at: 0 3024 3079 3891 4176
Size: 3024 55 812 285
Fragments arranged by size:
3024 812 285 55
BmgBI CAC'GTC
Cuts at: 0 2941 4176
Size: 2941 1235
BmrI ACTGGGnnnn_n'
Cuts at: 0 143 650 1554 4176
Size: 143 507 904 2622
Fragments arranged by size:
2622 904 507 143
BmtI G_CTAG'C
Cuts at: 0 3155 4176
Size: 3155 1021
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2821 4176
Size: 2821 1355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 1909 1930 3034 3055 3130 3178 3199
Size: 368 1541 21 1104 21 75 48 21
Cuts at: 3199 3553 4176
Size: 354 623
Fragments arranged by size:
1541 1104 623 368 354 75 48 21
21 21
Bpu10I CC'TnA_GC
Cuts at: 0 864 1678 2050 4028 4176
Size: 864 814 372 1978 148
Fragments arranged by size:
1978 864 814 372 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 2651 4118 4176
Size: 1148 1503 1467 58
Fragments arranged by size:
1503 1467 1148 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 671 1906 2092 4176
Size: 671 1235 186 2084
Fragments arranged by size:
2084 1235 671 186
BsaBI GATnn'nnATC
Cuts at: 0 1372 3398 4176
Size: 1372 2026 778
Fragments arranged by size:
2026 1372 778
BsaHI Gr'CG_yC
Cuts at: 0 653 3384 4176
Size: 653 2731 792
Fragments arranged by size:
2731 792 653
BsaWI w'CCGG_w
Cuts at: 0 623 784 4176
Size: 623 161 3392
Fragments arranged by size:
3392 623 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 52 82 2338 2368 2717 2747 3629 3659
Size: 52 30 2256 30 349 30 882 30
Cuts at: 3659 4176
Size: 517
Fragments arranged by size:
2256 882 517 349 52 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1978 2056 2269 2380 2461 3054 3869 4176
Size: 1978 78 213 111 81 593 815 307
Fragments arranged by size:
1978 815 593 307 213 111 81 78
BseYI C'CCAG_C
Cuts at: 0 1916 4176
Size: 1916 2260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 1960 4176
Size: 794 1166 2216
Fragments arranged by size:
2216 1166 794
BsiHKAI G_wGCw'C
Cuts at: 0 1567 3891 4034 4176
Size: 1567 2324 143 142
Fragments arranged by size:
2324 1567 143 142
BsmI GAATG_Cn'
Cuts at: 0 2387 3857 4176
Size: 2387 1470 319
Fragments arranged by size:
2387 1470 319
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1439 4176
Size: 1439 2737
Bsp1286I G_dGCh'C
Cuts at: 0 1567 3024 3079 3146 3891 4034 4176
Size: 1567 1457 55 67 745 143 142
Fragments arranged by size:
1567 1457 745 143 142 67 55
BspHI T'CATG_A
Cuts at: 0 989 4176
Size: 989 3187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 653 1017 2262 4176
Size: 653 364 1245 1914
Fragments arranged by size:
1914 1245 653 364
BsrBI CCG'CTC
Cuts at: 0 1893 3018 4176
Size: 1893 1125 1158
Fragments arranged by size:
1893 1158 1125
BsrDI GCAATG_nn'
Cuts at: 0 1503 2933 3818 4176
Size: 1503 1430 885 358
Fragments arranged by size:
1503 1430 885 358
BssSI C'ACGA_G
Cuts at: 0 2281 2420 4176
Size: 2281 139 1756
Fragments arranged by size:
2281 1756 139
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2277 4176
Size: 2277 1899
BstBI TT'CG_AA
Cuts at: 0 432 2477 4176
Size: 432 2045 1699
Fragments arranged by size:
2045 1699 432
BstYI r'GATC_y
Cuts at: 0 313 371 477 740 2576 3576 4176
Size: 313 58 106 263 1836 1000 600
Fragments arranged by size:
1836 1000 600 313 263 106 58
Bsu36I CC'TnA_GG
Cuts at: 0 1318 4106 4176
Size: 1318 2788 70
Fragments arranged by size:
2788 1318 70
BtgI C'CryG_G
Cuts at: 0 1457 4176
Size: 1457 2719
BtsI GCAGTG_nn'
Cuts at: 0 2924 4176
Size: 2924 1252
ClaI AT'CG_AT
Cuts at: 0 1376 4176
Size: 1376 2800
DraI TTT'AAA
Cuts at: 0 1418 2591 4176
Size: 1418 1173 1585
Fragments arranged by size:
1585 1418 1173
DrdI GACnn_nn'nnGTC
Cuts at: 0 389 4176
Size: 389 3787
EaeI y'GGCC_r
Cuts at: 0 17 3026 3777 4176
Size: 17 3009 751 399
Fragments arranged by size:
3009 751 399 17
EarI CTCTTCn'nnn_
Cuts at: 0 141 391 1049 1681 1687 2033 2077 2090
Size: 141 250 658 632 6 346 44 13
Cuts at: 2090 2291 2357 2365 3083 4176
Size: 201 66 8 718 1093
Fragments arranged by size:
1093 718 658 632 346 250 201 141
66 44 13 8 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 504 4176
Size: 504 3672
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 372 458 1207 1698 1909 1930 3034
Size: 368 4 86 749 491 211 21 1104
Cuts at: 3034 3055 3130 3178 3199 3373 3553 3986 4021
Size: 21 75 48 21 174 180 433 35
Cuts at: 4021 4176
Size: 155
Fragments arranged by size:
1104 749 491 433 368 211 180 174
155 86 75 48 35 21 21 21
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3181 4176
Size: 3181 995
EcoO109I rG'GnC_Cy
Cuts at: 0 541 1556 2376 2523 2800 3021 3269 4176
Size: 541 1015 820 147 277 221 248 907
Fragments arranged by size:
1015 907 820 541 277 248 221 147
EcoRI G'AATT_C
Cuts at: 0 2479 2920 3925 4176
Size: 2479 441 1005 251
Fragments arranged by size:
2479 1005 441 251
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 762 982 2881 4176
Size: 762 220 1899 1295
Fragments arranged by size:
1899 1295 762 220
HaeII r_GCGC'y
Cuts at: 0 3013 4176
Size: 3013 1163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 544 576 1521 1553 1741 1773 2596 2628
Size: 544 32 945 32 188 32 823 32
Cuts at: 2628 4176
Size: 1548
Fragments arranged by size:
1548 945 823 544 188 32 32 32
32
HincII GTy'rAC
Cuts at: 0 944 3675 3965 4176
Size: 944 2731 290 211
Fragments arranged by size:
2731 944 290 211
HindIII A'AGCT_T
Cuts at: 0 422 2227 4176
Size: 422 1805 1949
Fragments arranged by size:
1949 1805 422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 2151 2603 2741 4176
Size: 373 1778 452 138 1435
Fragments arranged by size:
1778 1435 452 373 138
MscI TGG'CCA
Cuts at: 0 3028 3779 4176
Size: 3028 751 397
Fragments arranged by size:
3028 751 397
MslI CAynn'nnrTG
Cuts at: 0 334 2281 2659 3754 4176
Size: 334 1947 378 1095 422
Fragments arranged by size:
1947 1095 422 378 334
MspA1I CmG'CkG
Cuts at: 0 2895 3219 3544 4176
Size: 2895 324 325 632
Fragments arranged by size:
2895 632 325 324
NdeI CA'TA_TG
Cuts at: 0 300 4176
Size: 300 3876
NheI G'CTAG_C
Cuts at: 0 3151 4176
Size: 3151 1025
PasI CC'CwG_GG
Cuts at: 0 3072 4176
Size: 3072 1104
PflMI CCAn_nnn'nTGG
Cuts at: 0 413 1262 4176
Size: 413 849 2914
Fragments arranged by size:
2914 849 413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 4176
Size: 52 32 4092
Fragments arranged by size:
4092 52 32
PpuMI rG'GwC_Cy
Cuts at: 0 1556 2376 2523 3269 4176
Size: 1556 820 147 746 907
Fragments arranged by size:
1556 907 820 746 147
PsiI TTA'TAA
Cuts at: 0 281 461 4176
Size: 281 180 3715
Fragments arranged by size:
3715 281 180
PspOMI G'GGCC_C
Cuts at: 0 3020 4176
Size: 3020 1156
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2708 2740 4176
Size: 2708 32 1436
Fragments arranged by size:
2708 1436 32
PstI C_TGCA'G
Cuts at: 0 1764 2078 2741 3059 3424 3435 4176
Size: 1764 314 663 318 365 11 741
Fragments arranged by size:
1764 741 663 365 318 314 11
PvuII CAG'CTG
Cuts at: 0 2895 3219 4176
Size: 2895 324 957
Fragments arranged by size:
2895 957 324
SanDI GG'GwC_CC
Cuts at: 0 1556 3269 4176
Size: 1556 1713 907
Fragments arranged by size:
1713 1556 907
SapI GCTCTTCn'nnn_
Cuts at: 0 2291 3083 4176
Size: 2291 792 1093
Fragments arranged by size:
2291 1093 792
ScaI AGT'ACT
Cuts at: 0 1041 1395 4176
Size: 1041 354 2781
Fragments arranged by size:
2781 1041 354
SexAI A'CCwGG_T
Cuts at: 0 2525 3326 4176
Size: 2525 801 850
Fragments arranged by size:
2525 850 801
SfcI C'TryA_G
Cuts at: 0 1760 2074 2725 2737 3055 3420 3431 4176
Size: 1760 314 651 12 318 365 11 745
Fragments arranged by size:
1760 745 651 365 318 314 12 11
SmlI C'TyrA_G
Cuts at: 0 1163 2666 4133 4176
Size: 1163 1503 1467 43
Fragments arranged by size:
1503 1467 1163 43
SpeI A'CTAG_T
Cuts at: 0 3975 4176
Size: 3975 201
SspI AAT'ATT
Cuts at: 0 822 1293 4176
Size: 822 471 2883
Fragments arranged by size:
2883 822 471
StyI C'CwwG_G
Cuts at: 0 966 1256 3668 4176
Size: 966 290 2412 508
Fragments arranged by size:
2412 966 508 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1744 2346 3757 3829 4176
Size: 1744 602 1411 72 347
Fragments arranged by size:
1744 1411 602 347 72
TatI w'GTAC_w
Cuts at: 0 573 1039 1393 2696 3424 4176
Size: 573 466 354 1303 728 752
Fragments arranged by size:
1303 752 728 573 466 354
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2338 2370 3629 3661 4176
Size: 2338 32 1259 32 515
Fragments arranged by size:
2338 1259 515 32 32
XbaI T'CTAG_A
Cuts at: 0 2614 4176
Size: 2614 1562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2232 4176
Size: 2232 1944
XmnI GAAnn'nnTTC
Cuts at: 0 1220 2387 4176
Size: 1220 1167 1789
Fragments arranged by size:
1789 1220 1167
ZraI GAC'GTC
Cuts at: 0 654 4176
Size: 654 3522
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AseI BanI BanII BbsI BbvCI
BcgI BclI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtsI ClaI DraI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII MmeI MscI MslI MspA1I NdeI NheI
PasI PflMI PpiI PpuMI PsiI PspOMI PsrI PstI
PvuII SanDI SapI ScaI SexAI SfcI SmlI SpeI
SspI StyI TaqII TatI TstI XbaI XcmI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AscI
AsiSI AvaI AvrII BaeI BamHI BbeI BciVI BfrBI
BglI BsaAI BsiEI BsiWI BspEI BsrFI BsrGI BssHII
BstEII BstXI BstZ17I BtgZI CspCI DraIII EagI EcoICRI
EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NgoMIV NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PshAI PspXI
PvuI RsrII SacI SacII SalI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI StuI SwaI
Tth111I XhoI XmaI