(Circular) (Six-base) MAPSORT of: pFN21AA1584.seq Check: 4353 from: 1 to: 7603 pFN21AA1584 7603 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3050 3050 Size: 7603 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5413 278 Size: 53 83 186 936 3877 2468 Fragments arranged by size: 3877 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4875 4946 1937 Size: 2938 71 4594 Fragments arranged by size: 4594 2938 71 Acc65I G'GTAC_C Cuts at: 4924 4924 Size: 7603 AclI AA'CG_TT Cuts at: 1563 5730 6103 1563 Size: 4167 373 3063 Fragments arranged by size: 4167 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2292 2529 2571 2586 2920 3510 3575 4754 Size: 660 237 42 15 334 590 65 1179 Cuts at: 4754 4776 5666 7050 7318 1632 Size: 22 890 1384 268 1917 Fragments arranged by size: 1917 1384 1179 890 660 590 334 268 237 65 42 22 15 AflII C'TTAA_G Cuts at: 820 1017 4096 820 Size: 197 3079 4327 Fragments arranged by size: 4327 3079 197 AflIII A'CryG_T Cuts at: 1203 3166 4495 6502 7479 1203 Size: 1963 1329 2007 977 1327 Fragments arranged by size: 2007 1963 1329 1327 977 AgeI A'CCGG_T Cuts at: 6434 6434 Size: 7603 AhdI GACnn_n'nnGTC Cuts at: 6332 6332 Size: 7603 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7603 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7603 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2662 2694 2662 Size: 32 7571 AlwNI CAG_nnn'CTG Cuts at: 2270 5346 6918 2270 Size: 3076 1572 2955 Fragments arranged by size: 3076 2955 1572 ApaLI G'TGCA_C Cuts at: 2193 5660 6816 2193 Size: 3467 1156 2980 Fragments arranged by size: 3467 2980 1156 ApoI r'AATT_y Cuts at: 1493 2284 2566 2795 2949 3119 3811 4270 4330 Size: 791 282 229 154 170 692 459 60 Cuts at: 4330 4912 5138 5290 7399 1493 Size: 582 226 152 2109 1697 Fragments arranged by size: 2109 1697 791 692 582 459 282 229 226 170 154 152 60 AseI AT'TA_AT Cuts at: 160 1032 6157 160 Size: 872 5125 1606 Fragments arranged by size: 5125 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7603 AvaI C'yCGr_G Cuts at: 1870 1943 4928 1870 Size: 73 2985 4545 Fragments arranged by size: 4545 2985 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4733 4766 4733 Size: 33 7570 BamHI G'GATC_C Cuts at: 2722 4933 5300 2722 Size: 2211 367 5025 Fragments arranged by size: 5025 2211 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4924 6379 618 Size: 325 217 627 3137 1455 1842 Fragments arranged by size: 3137 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4922 1391 Size: 3531 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2918 928 Size: 971 1019 5613 Fragments arranged by size: 5613 1019 971 BbvCI CC'TCA_GC Cuts at: 2572 2572 Size: 7603 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5794 5828 1903 Size: 34 3857 34 3678 Fragments arranged by size: 3857 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2410 4163 5495 6705 2410 Size: 1753 1332 1210 3308 Fragments arranged by size: 3308 1753 1332 1210 BclI T'GATC_A Cuts at: 1548 2850 4555 4645 4819 1548 Size: 1302 1705 90 174 4332 Fragments arranged by size: 4332 1705 1302 174 90 BfrBI ATG'CAT Cuts at: 3355 3843 3355 Size: 488 7115 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6214 136 Size: 107 122 71 631 605 4542 1525 Fragments arranged by size: 4542 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2303 7598 2303 Size: 5295 2308 BlpI GC'TnA_GC Cuts at: 5020 5020 Size: 7603 Bme1580I G_kGCm'C Cuts at: 1790 2197 5664 6820 1790 Size: 407 3467 1156 2573 Fragments arranged by size: 3467 2573 1156 407 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7603 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5334 6292 442 Size: 416 53 83 399 24 3917 958 1753 Fragments arranged by size: 3917 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2630 1056 Size: 1574 6029 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2580 4561 4860 6263 1239 Size: 1341 1981 299 1403 2579 Fragments arranged by size: 2579 1981 1403 1341 299 Bpu10I CC'TnA_GC Cuts at: 2572 2572 Size: 7603 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3023 4766 5728 6593 6891 3023 Size: 1743 962 865 298 3735 Fragments arranged by size: 3735 1743 962 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6266 882 Size: 617 4767 2219 Fragments arranged by size: 4767 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 5394 493 Size: 711 2687 1503 2702 Fragments arranged by size: 2702 2687 1503 711 BsaBI GATnn'nnATC Cuts at: 3247 4624 7597 3247 Size: 1377 2973 3253 Fragments arranged by size: 3253 2973 1377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5410 5792 6427 275 Size: 53 83 186 936 3877 382 635 1451 Fragments arranged by size: 3877 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6036 6434 6708 6855 7266 6036 Size: 398 274 147 411 6373 Fragments arranged by size: 6373 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7378 Fragments arranged by size: 7378 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 1186 Size: 865 6738 BseYI C'CCAG_C Cuts at: 2162 2543 3024 6806 7302 2162 Size: 381 481 3782 496 2463 Fragments arranged by size: 3782 2463 496 481 381 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4482 1163 Size: 573 2746 4284 Fragments arranged by size: 4284 2746 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5053 5814 6842 1540 Size: 275 186 3052 761 1028 2301 Fragments arranged by size: 3052 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2197 4170 5664 5749 6820 2197 Size: 1973 1494 85 1071 2980 Fragments arranged by size: 2980 1973 1494 1071 85 BsiWI C'GTAC_G Cuts at: 3891 3891 Size: 7603 BsmI GAATG_Cn' Cuts at: 2219 3831 4006 5114 5207 2219 Size: 1612 175 1108 93 4615 Fragments arranged by size: 4615 1612 1108 175 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2197 4170 4922 5664 5749 6820 1391 Size: 399 407 1973 752 742 85 1071 2174 Fragments arranged by size: 2174 1973 1071 752 742 407 399 85 BspEI T'CCGG_A Cuts at: 7266 7266 Size: 7603 BspHI T'CATG_A Cuts at: 5492 7222 5492 Size: 1730 5873 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2453 3050 4958 844 Size: 1609 597 1908 3489 Fragments arranged by size: 3489 1908 1609 597 BsrBI CCG'CTC Cuts at: 1394 1823 5490 1394 Size: 429 3667 3507 Fragments arranged by size: 3667 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6098 6272 65 Size: 1183 4850 174 1396 Fragments arranged by size: 4850 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6247 6434 1700 Size: 110 4437 187 2869 Fragments arranged by size: 4437 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3395 96 Size: 3299 4304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7603 BssSI C'ACGA_G Cuts at: 2367 5663 6675 2367 Size: 3296 1012 3295 Fragments arranged by size: 3296 3295 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7603 BstBI TT'CG_AA Cuts at: 6407 6407 Size: 7603 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7603 BstXI CCAn_nnnn'nTGG Cuts at: 4421 4421 Size: 7603 BstYI r'GATC_y Cuts at: 1972 2303 2722 4933 5300 5685 5702 7154 7240 Size: 331 419 2211 367 385 17 1452 86 Cuts at: 7240 7252 7598 1972 Size: 12 346 1977 Fragments arranged by size: 2211 1977 1452 419 385 367 346 331 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2048 513 Size: 552 939 44 6068 Fragments arranged by size: 6068 939 552 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6864 Fragments arranged by size: 6864 683 56 BtsI GCAGTG_nn' Cuts at: 3022 3049 3092 5119 5913 5933 3022 Size: 27 43 2027 794 20 4692 Fragments arranged by size: 4692 2027 794 43 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7603 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7568 DraI TTT'AAA Cuts at: 2929 4907 2929 Size: 1978 5625 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6610 809 Size: 507 5294 1802 Fragments arranged by size: 5294 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5050 5939 7568 Size: 54 1425 50 275 18 3220 889 1629 Cuts at: 7568 8 Size: 43 Fragments arranged by size: 3220 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5050 1537 Size: 275 3238 4090 Fragments arranged by size: 4090 3238 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 5533 1353 Size: 675 3505 3423 Fragments arranged by size: 3505 3423 675 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6190 6564 6710 387 Size: 1064 138 4601 374 146 1280 Fragments arranged by size: 4601 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2292 2529 2571 2580 2586 2920 3510 Size: 393 660 237 42 9 6 334 590 Cuts at: 3510 3575 4561 4754 4776 4860 5666 6263 7050 Size: 65 986 193 22 84 806 597 787 Cuts at: 7050 7318 1239 Size: 268 1524 Fragments arranged by size: 1524 986 806 787 660 597 590 393 334 268 237 193 84 65 42 22 9 6 EcoRI G'AATT_C Cuts at: 4912 5290 4912 Size: 378 7225 EcoRV GAT'ATC Cuts at: 2115 2115 Size: 7603 FspI TGC'GCA Cuts at: 6109 6109 Size: 7603 HaeII r_GCGC'y Cuts at: 1678 6750 1678 Size: 5072 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2288 2320 4552 4559 4584 Size: 32 135 32 716 32 2232 7 25 Cuts at: 4584 4591 6244 6276 6318 6350 1373 Size: 7 1653 32 42 32 2626 Fragments arranged by size: 2626 2232 1653 716 135 42 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2158 2237 4103 4947 5193 669 Size: 988 281 220 79 1866 844 246 3079 Fragments arranged by size: 3079 1866 988 844 281 246 220 79 HindIII A'AGCT_T Cuts at: 748 748 Size: 7603 HpaI GTT'AAC Cuts at: 2158 5193 2158 Size: 3035 4568 KpnI G_GTAC'C Cuts at: 4928 4928 Size: 7603 MfeI C'AATT_G Cuts at: 5202 5202 Size: 7603 MluI A'CGCG_T Cuts at: 7479 7479 Size: 7603 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2276 3511 3699 6717 6901 1305 Size: 971 1235 188 3018 184 2007 Fragments arranged by size: 3018 2007 1235 971 188 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5781 Fragments arranged by size: 5781 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5561 5920 6079 518 Size: 648 4395 359 159 2042 Fragments arranged by size: 4395 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2576 5019 5696 6844 7089 1606 Size: 970 2443 677 1148 245 2120 Fragments arranged by size: 2443 2120 1148 970 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2048 513 Size: 552 939 44 6068 Fragments arranged by size: 6068 939 552 44 NdeI CA'TA_TG Cuts at: 387 3183 3217 3951 387 Size: 2796 34 734 4039 Fragments arranged by size: 4039 2796 734 34 NheI G'CTAG_C Cuts at: 1052 2626 1052 Size: 1574 6029 NotI GC'GGCC_GC Cuts at: 5050 5050 Size: 7603 NsiI A_TGCA'T Cuts at: 3357 3845 3357 Size: 488 7115 NspI r_CATG'y Cuts at: 1132 1229 3170 3426 4961 6506 1132 Size: 97 1941 256 1535 1545 2229 Fragments arranged by size: 2229 1941 1545 1535 256 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7603 PciI A'CATG_T Cuts at: 3166 6502 3166 Size: 3336 4267 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7603 PfoI T'CCnGG_A Cuts at: 2898 3499 4837 2898 Size: 601 1338 5664 Fragments arranged by size: 5664 1338 601 PmeI GTTT'AAAC Cuts at: 4907 4907 Size: 7603 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2461 2493 5672 5704 7185 7217 2461 Size: 32 3179 32 1481 32 2847 Fragments arranged by size: 3179 2847 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7603 PsiI TTA'TAA Cuts at: 3470 5173 3470 Size: 1703 5900 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3496 3528 3496 Size: 32 7571 PstI C_TGCA'G Cuts at: 830 3414 4955 830 Size: 2584 1541 3478 Fragments arranged by size: 3478 2584 1541 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7603 PvuII CAG'CTG Cuts at: 2576 2576 Size: 7603 SalI G'TCGA_C Cuts at: 1936 4945 1936 Size: 3009 4594 SbfI CC_TGCA'GG Cuts at: 4955 4955 Size: 7603 ScaI AGT'ACT Cuts at: 5851 7563 5851 Size: 1712 5891 SexAI A'CCwGG_T Cuts at: 2344 3202 2344 Size: 858 6745 SfcI C'TryA_G Cuts at: 826 1045 3410 3901 3928 4951 6086 6767 6958 Size: 219 2365 491 27 1023 1135 681 191 Cuts at: 6958 826 Size: 1471 Fragments arranged by size: 2365 1471 1135 1023 681 491 219 191 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7603 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7603 SmaI CCC'GGG Cuts at: 1872 4930 1872 Size: 3058 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3002 4096 4745 5707 6608 6870 Size: 197 926 1059 1094 649 962 901 262 Cuts at: 6870 820 Size: 1553 Fragments arranged by size: 1553 1094 1059 962 926 901 649 262 197 SnaBI TAC'GTA Cuts at: 493 3891 5394 493 Size: 3398 1503 2702 Fragments arranged by size: 3398 2702 1503 SpeI A'CTAG_T Cuts at: 152 152 Size: 7603 SphI G_CATG'C Cuts at: 1132 4961 1132 Size: 3829 3774 SspI AAT'ATT Cuts at: 5 52 2143 4456 4606 5527 5 Size: 47 2091 2313 150 921 2081 Fragments arranged by size: 2313 2091 2081 921 150 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7603 StyI C'CwwG_G Cuts at: 513 1065 2004 2048 2437 3605 5042 513 Size: 552 939 44 389 1168 1437 3074 Fragments arranged by size: 3074 1437 1168 939 552 389 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5630 5647 5800 5985 6430 325 Size: 1255 4050 17 153 185 445 1498 Fragments arranged by size: 4050 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2804 3101 3395 Size: 274 80 33 51 1443 827 297 294 Cuts at: 3395 5849 7561 96 Size: 2454 1712 138 Fragments arranged by size: 2454 1712 1443 827 297 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7563 7595 7563 Size: 32 7571 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7603 XbaI T'CTAG_A Cuts at: 2300 4939 2300 Size: 2639 4964 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7603 XmaI C'CCGG_G Cuts at: 1870 4928 1870 Size: 3058 4545 XmnI GAAnn'nnTTC Cuts at: 3168 5732 3168 Size: 2564 5039 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5411 276 Size: 53 83 186 936 3877 2468 Fragments arranged by size: 3877 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BplI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI