Restriction map for
pFN21AA1489
(Circular) (Six-base) MAPSORT of: pFN21AA1489.seq Check: 4190 from: 1 to: 6574
pFN21AA1489 6574 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4384 278
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3917 1937
Size: 1980 4594
Acc65I G'GTAC_C
Cuts at: 3895 3895
Size: 6574
AclI AA'CG_TT
Cuts at: 1563 2359 4701 5074 1563
Size: 796 2342 373 3063
Fragments arranged by size:
3063 2342 796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2416 2814 3008 3019 3375 3474 4637 6021
Size: 784 398 194 11 356 99 1163 1384
Cuts at: 6021 6289 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1163 784 398 356 268 194
99 11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6377
AflIII A'CryG_T
Cuts at: 1203 2156 2505 3712 5473 6450 1203
Size: 953 349 1207 1761 977 1327
Fragments arranged by size:
1761 1327 1207 977 953 349
AgeI A'CCGG_T
Cuts at: 3681 5405 3681
Size: 1724 4850
AhdI GACnn_n'nnGTC
Cuts at: 3685 5303 3685
Size: 1618 4956
AleI CACnn'nnGTG
Cuts at: 1166 2489 1166
Size: 1323 5251
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3285 1184
Size: 2101 4473
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2465 2497 2465
Size: 32 6542
AlwNI CAG_nnn'CTG
Cuts at: 3497 4317 5889 3497
Size: 820 1572 4182
Fragments arranged by size:
4182 1572 820
ApaLI G'TGCA_C
Cuts at: 4631 5787 4631
Size: 1156 5418
ApoI r'AATT_y
Cuts at: 1493 2126 3414 3504 3598 3883 4109 4261 6370
Size: 633 1288 90 94 285 226 152 2109
Cuts at: 6370 1493
Size: 1697
Fragments arranged by size:
2109 1697 1288 633 285 226 152 94
90
AseI AT'TA_AT
Cuts at: 160 1032 5128 160
Size: 872 4096 1606
Fragments arranged by size:
4096 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 3899 1870
Size: 73 1956 4545
Fragments arranged by size:
4545 1956 73
BamHI G'GATC_C
Cuts at: 3904 4271 3904
Size: 367 6207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3895 5350 618
Size: 325 217 627 2108 1455 1842
Fragments arranged by size:
2108 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3893 1391
Size: 2502 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2637 3480 928
Size: 971 738 843 4022
Fragments arranged by size:
4022 971 843 738
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4765 4799 1903
Size: 34 2828 34 3678
Fragments arranged by size:
3678 2828 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4466 5676 4466
Size: 1210 5364
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6574
BfrBI ATG'CAT
Cuts at: 2270 2270
Size: 6574
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5185 136
Size: 107 122 71 631 605 3513 1525
Fragments arranged by size:
3513 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6569 6569
Size: 6574
BlpI GC'TnA_GC
Cuts at: 3991 3991
Size: 6574
Bme1580I G_kGCm'C
Cuts at: 1790 2123 4635 5791 1790
Size: 333 2512 1156 2573
Fragments arranged by size:
2573 2512 1156 333
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6574
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2629 4305 5263
Size: 416 53 83 399 24 1212 1676 958
Cuts at: 5263 442
Size: 1753
Fragments arranged by size:
1753 1676 1212 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5234 1239
Size: 3995 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1998 4699 5564 5862 1998
Size: 2701 865 298 2710
Fragments arranged by size:
2710 2701 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3693 5237 882
Size: 617 2194 1544 2219
Fragments arranged by size:
2219 2194 1544 617
BsaAI yAC'GTr
Cuts at: 493 1204 4365 493
Size: 711 3161 2702
Fragments arranged by size:
3161 2702 711
BsaBI GATnn'nnATC
Cuts at: 6568 6568
Size: 6574
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4381 4763 5398 275
Size: 53 83 186 936 2848 382 635 1451
Fragments arranged by size:
2848 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3681 5007 5405 5679 5826 6237 3681
Size: 1326 398 274 147 411 4018
Fragments arranged by size:
4018 1326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2465 2495 3156 3186 3840
Size: 30 165 30 1062 30 661 30 654
Cuts at: 3840 3870 1178
Size: 30 3882
Fragments arranged by size:
3882 1062 661 654 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3177 1186
Size: 1991 4583
BseYI C'CCAG_C
Cuts at: 5777 6273 5777
Size: 496 6078
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3291 1163
Size: 573 1555 4446
Fragments arranged by size:
4446 1555 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4024 4785 5813 1540
Size: 275 186 2023 761 1028 2301
Fragments arranged by size:
2301 2023 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2478 4635 4720 5791 2478
Size: 2157 85 1071 3261
Fragments arranged by size:
3261 2157 1071 85
BsmI GAATG_Cn'
Cuts at: 4085 4178 4085
Size: 93 6481
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2478 3893 4635 4720 5791 1391
Size: 399 333 355 1415 742 85 1071 2174
Fragments arranged by size:
2174 1415 1071 742 399 355 333 85
BspEI T'CCGG_A
Cuts at: 6237 6237
Size: 6574
BspHI T'CATG_A
Cuts at: 3286 4463 6193 3286
Size: 1177 1730 3667
Fragments arranged by size:
3667 1730 1177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3929 844
Size: 3085 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4461 1394
Size: 429 2638 3507
Fragments arranged by size:
3507 2638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2295 3269 5069 5243 65
Size: 1183 1047 974 1800 174 1396
Fragments arranged by size:
1800 1396 1183 1047 974 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3681 5218 5405 1700
Size: 110 1871 1537 187 2869
Fragments arranged by size:
2869 1871 1537 187 110
BsrGI T'GTAC_A
Cuts at: 96 2218 96
Size: 2122 4452
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6574
BssSI C'ACGA_G
Cuts at: 4634 5646 4634
Size: 1012 5562
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6574
BstBI TT'CG_AA
Cuts at: 5378 5378
Size: 6574
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6574
BstYI r'GATC_y
Cuts at: 1972 3904 4271 4656 4673 6125 6211 6223 6569
Size: 1932 367 385 17 1452 86 12 346
Cuts at: 6569 1972
Size: 1977
Fragments arranged by size:
1977 1932 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2225 2225
Size: 6574
BtgI C'CryG_G
Cuts at: 513 1065 3788 513
Size: 552 2723 3299
Fragments arranged by size:
3299 2723 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5835
Fragments arranged by size:
5835 683 56
BtsI GCAGTG_nn'
Cuts at: 2484 4090 4884 4904 2484
Size: 1606 794 20 4154
Fragments arranged by size:
4154 1606 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6539
DraI TTT'AAA
Cuts at: 2562 3878 2562
Size: 1316 5258
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3379 5581 809
Size: 507 2063 2202 1802
Fragments arranged by size:
2202 2063 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3360 4021 4910
Size: 54 1425 50 275 18 1530 661 889
Cuts at: 4910 6539 8
Size: 1629 43
Fragments arranged by size:
1629 1530 1425 889 661 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4021 1537
Size: 275 2209 4090
Fragments arranged by size:
4090 2209 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2435 2860 3774 3825 4504 1353
Size: 1082 425 914 51 679 3423
Fragments arranged by size:
3423 1082 914 679 425 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5161 5535 5681 387
Size: 1064 138 3572 374 146 1280
Fragments arranged by size:
3572 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2416 2814 3008 3019 3375 3474 4637
Size: 393 784 398 194 11 356 99 1163
Cuts at: 4637 5234 6021 6289 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1163 787 784 597 398 393 356
268 194 99 11
EcoO109I rG'GnC_Cy
Cuts at: 3381 3381
Size: 6574
EcoRI G'AATT_C
Cuts at: 2126 3883 4261 2126
Size: 1757 378 4439
Fragments arranged by size:
4439 1757 378
EcoRV GAT'ATC
Cuts at: 2956 2956
Size: 6574
FspI TGC'GCA
Cuts at: 5080 5080
Size: 6574
HaeII r_GCGC'y
Cuts at: 1678 5721 1678
Size: 4043 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3671 3703 5215 5247 5289
Size: 32 135 32 2099 32 1512 32 42
Cuts at: 5289 5321 1373
Size: 32 2626
Fragments arranged by size:
2626 2099 1512 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3918 4164 669
Size: 988 281 1980 246 3079
Fragments arranged by size:
3079 1980 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6574
HpaI GTT'AAC
Cuts at: 4164 4164
Size: 6574
KpnI G_GTAC'C
Cuts at: 3899 3899
Size: 6574
MfeI C'AATT_G
Cuts at: 3156 4173 3156
Size: 1017 5557
MluI A'CGCG_T
Cuts at: 2505 6450 2505
Size: 3945 2629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 5688 5872 1305
Size: 732 3651 184 2007
Fragments arranged by size:
3651 2007 732 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3362 10
Size: 54 1425 343 1530 3222
Fragments arranged by size:
3222 1530 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 2489 3299 3633 4532 4891 5050
Size: 648 1017 306 810 334 899 359 159
Cuts at: 5050 518
Size: 2042
Fragments arranged by size:
2042 1017 899 810 648 359 334 306
159
MspA1I CmG'CkG
Cuts at: 1606 2518 2912 3395 3990 4667 5815 6060 1606
Size: 912 394 483 595 677 1148 245 2120
Fragments arranged by size:
2120 1148 912 677 595 483 394 245
NcoI C'CATG_G
Cuts at: 513 1065 3788 513
Size: 552 2723 3299
Fragments arranged by size:
3299 2723 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6574
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 4021 4021
Size: 6574
NsiI A_TGCA'T
Cuts at: 2272 2272
Size: 6574
NspI r_CATG'y
Cuts at: 1132 1229 2160 3932 5477 1132
Size: 97 931 1772 1545 2229
Fragments arranged by size:
2229 1772 1545 931 97
PacI TTA_AT'TAA
Cuts at: 2379 2379
Size: 6574
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6574
PciI A'CATG_T
Cuts at: 2156 5473 2156
Size: 3317 3257
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2786 1108
Size: 1678 4896
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2465 2497 4643 4675 6156 6188 2465
Size: 32 2146 32 1481 32 2851
Fragments arranged by size:
2851 2146 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3381 3381
Size: 6574
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6574
PsiI TTA'TAA
Cuts at: 4144 4144
Size: 6574
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 6542
PstI C_TGCA'G
Cuts at: 830 3400 3926 830
Size: 2570 526 3478
Fragments arranged by size:
3478 2570 526
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 2518 2912 3395 2518
Size: 394 483 5697
Fragments arranged by size:
5697 483 394
SalI G'TCGA_C
Cuts at: 1936 3916 1936
Size: 1980 4594
SbfI CC_TGCA'GG
Cuts at: 3926 3926
Size: 6574
ScaI AGT'ACT
Cuts at: 4822 6534 4822
Size: 1712 4862
SexAI A'CCwGG_T
Cuts at: 3080 3080
Size: 6574
SfcI C'TryA_G
Cuts at: 826 1045 2304 2336 3396 3477 3870 3922 5057
Size: 219 1259 32 1060 81 393 52 1135
Cuts at: 5057 5738 5929 826
Size: 681 191 1471
Fragments arranged by size:
1471 1259 1135 1060 681 393 219 191
81 52 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6574
SmaI CCC'GGG
Cuts at: 1872 3901 1872
Size: 2029 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2013 4678 5579 5841 820
Size: 197 926 70 2665 901 262 1553
Fragments arranged by size:
2665 1553 926 901 262 197 70
SnaBI TAC'GTA
Cuts at: 493 4365 493
Size: 3872 2702
SpeI A'CTAG_T
Cuts at: 152 3374 152
Size: 3222 3352
SphI G_CATG'C
Cuts at: 1132 3932 1132
Size: 2800 3774
SspI AAT'ATT
Cuts at: 5 52 2372 4498 5
Size: 47 2320 2126 2081
Fragments arranged by size:
2320 2126 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6574
StyI C'CwwG_G
Cuts at: 513 1065 3335 3788 4013 513
Size: 552 2270 453 225 3074
Fragments arranged by size:
3074 2270 552 453 225
SwaI ATTT'AAAT
Cuts at: 2562 2562
Size: 6574
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2734 4601 4618 4771 4956 5401 325
Size: 1255 1154 1867 17 153 185 445 1498
Fragments arranged by size:
1867 1498 1255 1154 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2218 3321 3715
Size: 274 80 33 51 1443 241 1103 394
Cuts at: 3715 4820 6532 96
Size: 1105 1712 138
Fragments arranged by size:
1712 1443 1105 1103 394 274 241 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6534 6566 6534
Size: 32 6542
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6574
XbaI T'CTAG_A
Cuts at: 3910 3910
Size: 6574
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6574
XmaI C'CCGG_G
Cuts at: 1870 3899 1870
Size: 2029 4545
XmnI GAAnn'nnTTC
Cuts at: 2583 4703 2583
Size: 2120 4454
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4382 276
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PfoI PmlI PspOMI PspXI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI XcmI