Restriction map for
pFN21AA1477
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AclI AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BciVI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsiEI BsiWI BsmI BspLU11I BssHII
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I CelII CfoI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HhaI HinP1I HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MslI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccI 299 GTCTAC GT^[AC][GT]AC
AccII 1979 CGCG CG^CG
AccIII 915 TCCGGA T^CCGGA
AflII 481 CTTAAG C^TTAAG
AflIII 234 ACACGT A^C[GA][CT]GT
AocI 1252 CCTCAGG CC^T[ACGT]AGG
AspHI 1755 GAGCTC G[AT]GC[AT]^C
BalI 708 TGGCCA TGG^CCA
BanII 1755 GAGCTC G[GA]GC[CT]^C
BbsI 2007 GAAGAC GAAGAC(2/6)
BbvCI 1568 CCTCAGC CCTCAGC(-5/-2)
BcgI 1344 CGAGATGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1203 TGATCA T^GATCA
BfrI 481 CTTAAG C^TTAAG
BmyI 1755 GAGCTC G[AGT]GC[ACT]^C
BpuAI 2007 GAAGAC GAAGAC(2/6)
BsaAI 1474 TACGTA [CT]AC^GT[GA]
BseAI 915 TCCGGA T^CCGGA
BseRI 1285 GAGGAG GAGGAG(10/8)
BsgI 2146 GTGCAG GTGCAG(16/14)
BsiHKAI 1755 GAGCTC G[AT]GC[AT]^C
BsmBI 2123 GAGACG CGTCTC(1/5)
Bsp1286I 1755 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 441 TGTACA T^GTACA
BspEI 915 TCCGGA T^CCGGA
BspHI 876 TCATGA T^CATGA
BspMI 1824 ACCTGC ACCTGC(4/8)
BsrBI 34 GAGCGG CCGCTC(-3/-3)
BsrFI 1661 ACCGGC [GA]^CCGG[CT]
BsrGI 441 TGTACA T^GTACA
BstEII 951 GGTCACC G^GT[ACGT]ACC
BstNI 1666 CCTGG CC^[AT]GG
BstPI 951 GGTCACC G^GT[ACGT]ACC
BstUI 1979 CGCG CG^CG
Bsu36I 1252 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1661 ACCGGC [GA]^CCGG[CT]
DraI 717 TTTAAA TTT^AAA
DraII 1099 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 943 GACATTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 708 TGGCCA [CT]^GGCC[GA]
Eco81I 1252 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1099 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 951 GGTCACC G^GT[ACGT]ACC
EcoRII 1666 CCTGG ^CC[AT]GG
FbaI 1203 TGATCA T^GATCA
HincII 1878 GTCAAC GT[CT]^[GA]AC
HindII 1878 GTCAAC GT[CT]^[GA]AC
HindIII 1655 AAGCTT A^AGCTT
MluNI 708 TGGCCA TGG^CCA
MroI 915 TCCGGA T^CCGGA
MscI 708 TGGCCA TGG^CCA
MspA1I 1189 CAGCGG C[AC]G^C[GT]G
MvaI 1666 CCTGG CC^[AT]GG
MvnI 1979 CGCG CG^CG
NdeI 1445 CATATG CA^TATG
NspI 1860 ACATGC [GA]CATG^[CT]
PpuMI 1099 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 1538 CTGCAG CTGCA^G
RcaI 876 TCATGA T^CATGA
SacI 1755 GAGCTC GAGCT^C
SapI 1544 GCTCTTC GCTCTTC(1/4)
ScaI 1717 AGTACT AGT^ACT
SfcI 1538 CTGCAG C^T[GA][CT]AG
SnaBI 1474 TACGTA TAC^GTA
SpeI 2014 ACTAGT A^CTAGT
SspBI 441 TGTACA T^GTACA
SspI 517 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 12 GACGCC G[GA]^CG[CT]C
664 GGCGTC
AlwI 913 GATCC GGATC(4/5)
1333 GGATC
Asp700I 490 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
787 GAAAATCTTC
AvaI 607 CCCGAG C^[CT]CG[GA]G
1748 CCCGAG
BpmI 1727 CTCCAG CTGGAG(16/14)
1418 CTGGAG
BsaHI 12 GACGCC G[GA]^CG[CT]C
664 GGCGTC
BsaWI 915 TCCGGA [AT]^CCGG[AT]
1733 ACCGGA
BsmAI 1949 GAGAC GTCTC(1/5)
2123 GAGAC
BsoBI 607 CCCGAG C^[CT]CG[GA]G
1748 CCCGAG
BspDI 857 ATCGAT AT^CGAT
1469 ATCGAT
BssSI 968 CACGAG CACGAG(-5/-1)
2143 CTCGTG
ClaI 857 ATCGAT AT^CGAT
1469 ATCGAT
EcoNI 349 CCTAATATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1360 CCTCTTGTAGG
FauI 1580 CCCGC CCCGC(4/6)
36 GCGGG
Hin1I 12 GACGCC G[GA]^CG[CT]C
664 GGCGTC
MwoI 1788 GCTCCGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1980 GCGTTCTTTGC
PleI 1725 GACTC GAGTC(4/5)
657 GAGTC
SmlI 481 CTTAAG C^T[CT][GA]AG
1758 CTCAAG
XcmI 215 CCAAAGTCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2012 CCACTAGTTCAATGG
XmnI 490 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
787 GAAAATCTTC
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BcnI BfaI BsaJI
BsiYI BslI BsmFI BsrDI BsrI
BssKI BstF5I Cac8I Cfr13I DdeI
DpnII DsaI EarI EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspI NciI NcoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I