(Circular) (Six-base) MAPSORT of: pFN21AA1477.seq Check: 9370 from: 1 to: 6979 pFN21AA1477 6979 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3838 3838 Size: 6979 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4789 278 Size: 53 83 186 936 3253 2468 Fragments arranged by size: 3253 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2305 4322 1937 Size: 368 2017 4594 Fragments arranged by size: 4594 2017 368 Acc65I G'GTAC_C Cuts at: 4300 4300 Size: 6979 AclI AA'CG_TT Cuts at: 1563 5106 5479 1563 Size: 3543 373 3063 Fragments arranged by size: 3543 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2667 2822 3125 3179 3678 5042 6426 6694 Size: 1035 155 303 54 499 1364 1384 268 Cuts at: 6694 1632 Size: 1917 Fragments arranged by size: 1917 1384 1364 1035 499 303 268 155 54 AflII C'TTAA_G Cuts at: 820 1017 2486 820 Size: 197 1469 5313 Fragments arranged by size: 5313 1469 197 AflIII A'CryG_T Cuts at: 1203 2239 5878 6855 1203 Size: 1036 3639 977 1327 Fragments arranged by size: 3639 1327 1036 977 AgeI A'CCGG_T Cuts at: 5810 5810 Size: 6979 AhdI GACnn_n'nnGTC Cuts at: 5708 5708 Size: 6979 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6979 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6979 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2073 2105 2073 Size: 32 6947 AlwNI CAG_nnn'CTG Cuts at: 4722 6294 4722 Size: 1572 5407 ApaLI G'TGCA_C Cuts at: 5036 6192 5036 Size: 1156 5823 ApoI r'AATT_y Cuts at: 1493 2212 2423 2555 2719 3059 3914 4288 4514 Size: 719 211 132 164 340 855 374 226 Cuts at: 4514 4666 6775 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 855 719 374 340 226 211 164 152 132 AseI AT'TA_AT Cuts at: 160 1032 5533 160 Size: 872 4501 1606 Fragments arranged by size: 4501 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6979 AvaI C'yCGr_G Cuts at: 1870 1943 2612 3753 4304 1870 Size: 73 669 1141 551 4545 Fragments arranged by size: 4545 1141 669 551 73 BamHI G'GATC_C Cuts at: 4309 4676 4309 Size: 367 6612 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4300 5755 618 Size: 325 217 627 2513 1455 1842 Fragments arranged by size: 2513 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3764 4298 1391 Size: 2373 534 4072 Fragments arranged by size: 4072 2373 534 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4019 928 Size: 971 2120 3888 Fragments arranged by size: 3888 2120 971 BbvCI CC'TCA_GC Cuts at: 3574 3574 Size: 6979 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3338 3372 5170 5204 1903 Size: 34 1401 34 1798 34 3678 Fragments arranged by size: 3678 1798 1401 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4871 6081 4871 Size: 1210 5769 BclI T'GATC_A Cuts at: 1548 3208 1548 Size: 1660 5319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5590 136 Size: 107 122 71 631 605 337 3581 1525 Fragments arranged by size: 3581 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6974 6974 Size: 6979 BlpI GC'TnA_GC Cuts at: 4396 4396 Size: 6979 Bme1580I G_kGCm'C Cuts at: 1790 5040 6196 1790 Size: 3250 1156 2573 Fragments arranged by size: 3250 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6979 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3108 4710 5668 Size: 416 53 83 399 24 1691 1602 958 Cuts at: 5668 442 Size: 1753 Fragments arranged by size: 1753 1691 1602 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6979 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3444 3717 5639 1239 Size: 2205 273 1922 2579 Fragments arranged by size: 2579 2205 1922 273 Bpu10I CC'TnA_GC Cuts at: 3574 3574 Size: 6979 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3748 5104 5969 6267 3748 Size: 1356 865 298 4460 Fragments arranged by size: 4460 1356 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5642 882 Size: 617 4143 2219 Fragments arranged by size: 4143 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3481 4770 493 Size: 711 2277 1289 2702 Fragments arranged by size: 2702 2277 1289 711 BsaBI GATnn'nnATC Cuts at: 6973 6973 Size: 6979 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2670 4786 5168 Size: 53 83 186 936 485 652 2116 382 Cuts at: 5168 5803 275 Size: 635 1451 Fragments arranged by size: 2116 1451 936 652 635 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2920 3738 5412 5810 6084 6231 6642 2920 Size: 818 1674 398 274 147 411 3257 Fragments arranged by size: 3257 1674 818 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3715 3745 1178 Size: 30 165 30 2312 30 4412 Fragments arranged by size: 4412 2312 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3305 1186 Size: 2119 4860 BseYI C'CCAG_C Cuts at: 6182 6678 6182 Size: 496 6483 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4172 1163 Size: 573 2436 3970 Fragments arranged by size: 3970 2436 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4429 5190 6218 1540 Size: 275 186 2428 761 1028 2301 Fragments arranged by size: 2428 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3764 5040 5125 6196 3764 Size: 1276 85 1071 4547 Fragments arranged by size: 4547 1276 1071 85 BsmI GAATG_Cn' Cuts at: 4490 4583 4490 Size: 93 6886 BsmBI CGTCTCn'nnnn_ Cuts at: 4122 4122 Size: 6979 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3764 4298 5040 5125 6196 1391 Size: 399 1974 534 742 85 1071 2174 Fragments arranged by size: 2174 1974 1071 742 534 399 85 BspEI T'CCGG_A Cuts at: 2920 6642 2920 Size: 3722 3257 BspHI T'CATG_A Cuts at: 2881 4868 6598 2881 Size: 1987 1730 3262 Fragments arranged by size: 3262 1987 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3838 4334 844 Size: 2994 496 3489 Fragments arranged by size: 3489 2994 496 BsrBI CCG'CTC Cuts at: 1394 1823 2041 4866 1394 Size: 429 218 2825 3507 Fragments arranged by size: 3507 2825 429 218 BsrDI GCAATG_nn' Cuts at: 65 1248 2021 3485 4245 5474 5648 65 Size: 1183 773 1464 760 1229 174 1396 Fragments arranged by size: 1464 1396 1229 1183 773 760 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3666 5623 5810 1700 Size: 110 1856 1957 187 2869 Fragments arranged by size: 2869 1957 1856 187 110 BsrGI T'GTAC_A Cuts at: 96 2446 96 Size: 2350 4629 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6979 BssSI C'ACGA_G Cuts at: 2973 4148 5039 6051 2973 Size: 1175 891 1012 3901 Fragments arranged by size: 3901 1175 1012 891 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6979 BstBI TT'CG_AA Cuts at: 5783 5783 Size: 6979 BstEII G'GTnAC_C Cuts at: 1184 2956 1184 Size: 1772 5207 BstYI r'GATC_y Cuts at: 1972 4309 4676 5061 5078 6530 6616 6628 6974 Size: 2337 367 385 17 1452 86 12 346 Cuts at: 6974 1972 Size: 1977 Fragments arranged by size: 2337 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3258 3258 Size: 6979 BtgI C'CryG_G Cuts at: 513 1065 2389 2961 3781 4123 513 Size: 552 1324 572 820 342 3369 Fragments arranged by size: 3369 1324 820 572 552 342 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6240 Fragments arranged by size: 6240 683 56 BtsI GCAGTG_nn' Cuts at: 3632 4160 4495 5289 5309 3632 Size: 528 335 794 20 5302 Fragments arranged by size: 5302 794 528 335 20 ClaI AT'CG_AT Cuts at: 1554 2863 3475 1554 Size: 1309 612 5058 Fragments arranged by size: 5058 1309 612 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6944 DraI TTT'AAA Cuts at: 2724 4283 2724 Size: 1559 5420 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2954 5986 809 Size: 507 1638 3032 1802 Fragments arranged by size: 3032 1802 1638 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2713 4426 5315 Size: 54 1425 50 275 18 883 1713 889 Cuts at: 5315 6944 8 Size: 1629 43 Fragments arranged by size: 1713 1629 1425 889 883 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4426 1537 Size: 275 2614 4090 Fragments arranged by size: 4090 2614 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2880 3144 3556 3957 4909 1353 Size: 1527 264 412 401 952 3423 Fragments arranged by size: 3423 1527 952 412 401 264 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5566 5940 6086 387 Size: 1064 138 3977 374 146 1280 Fragments arranged by size: 3977 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3762 3762 Size: 6979 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2667 2822 3125 3179 3444 3678 3717 Size: 393 1035 155 303 54 265 234 39 Cuts at: 3717 5042 5639 6426 6694 1239 Size: 1325 597 787 268 1524 Fragments arranged by size: 1524 1325 1035 787 597 393 303 268 265 234 155 54 39 EcoNI CCTnn'n_nnAGG Cuts at: 2358 3369 2358 Size: 1011 5968 EcoO109I rG'GnC_Cy Cuts at: 3105 3105 Size: 6979 EcoRI G'AATT_C Cuts at: 4288 4666 4288 Size: 378 6601 FspI TGC'GCA Cuts at: 5485 5485 Size: 6979 HaeII r_GCGC'y Cuts at: 1678 6126 1678 Size: 4448 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2106 2138 2332 2364 3457 Size: 32 135 32 534 32 194 32 1093 Cuts at: 3457 3489 5620 5652 5694 5726 1373 Size: 32 2131 32 42 32 2626 Fragments arranged by size: 2626 2131 1093 534 194 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3885 4323 4569 669 Size: 988 281 1947 438 246 3079 Fragments arranged by size: 3079 1947 988 438 281 246 HindIII A'AGCT_T Cuts at: 748 3660 748 Size: 2912 4067 HpaI GTT'AAC Cuts at: 4569 4569 Size: 6979 KpnI G_GTAC'C Cuts at: 4304 4304 Size: 6979 MfeI C'AATT_G Cuts at: 4578 4578 Size: 6979 MluI A'CGCG_T Cuts at: 6855 6855 Size: 6979 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3316 6093 6277 1305 Size: 2011 2777 184 2007 Fragments arranged by size: 2777 2011 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2715 10 Size: 54 1425 343 883 4274 Fragments arranged by size: 4274 1425 883 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4937 5296 5455 518 Size: 648 3771 359 159 2042 Fragments arranged by size: 3771 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3196 4395 5072 6220 6465 1606 Size: 1590 1199 677 1148 245 2120 Fragments arranged by size: 2120 1590 1199 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2389 2961 3781 4123 513 Size: 552 1324 572 820 342 3369 Fragments arranged by size: 3369 1324 820 572 552 342 NdeI CA'TA_TG Cuts at: 387 3451 387 Size: 3064 3915 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6979 NotI GC'GGCC_GC Cuts at: 4426 4426 Size: 6979 NspI r_CATG'y Cuts at: 1132 1229 3869 4337 5882 1132 Size: 97 2640 468 1545 2229 Fragments arranged by size: 2640 2229 1545 468 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6979 PciI A'CATG_T Cuts at: 5878 5878 Size: 6979 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6979 PfoI T'CCnGG_A Cuts at: 3795 3795 Size: 6979 PmeI GTTT'AAAC Cuts at: 4283 4283 Size: 6979 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5048 5080 6561 6593 5048 Size: 32 1481 32 5434 Fragments arranged by size: 5434 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3105 3105 Size: 6979 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6979 PsiI TTA'TAA Cuts at: 3819 4549 3819 Size: 730 6249 PstI C_TGCA'G Cuts at: 830 3547 4331 830 Size: 2717 784 3478 Fragments arranged by size: 3478 2717 784 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6979 SacI G_AGCT'C Cuts at: 3764 3764 Size: 6979 SalI G'TCGA_C Cuts at: 1936 4321 1936 Size: 2385 4594 SapI GCTCTTCn'nnn_ Cuts at: 3556 3556 Size: 6979 SbfI CC_TGCA'GG Cuts at: 4331 4331 Size: 6979 ScaI AGT'ACT Cuts at: 3724 5227 6939 3724 Size: 1503 1712 3764 Fragments arranged by size: 3764 1712 1503 SfcI C'TryA_G Cuts at: 826 1045 3543 4327 5462 6143 6334 826 Size: 219 2498 784 1135 681 191 1471 Fragments arranged by size: 2498 1471 1135 784 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6979 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6979 SmaI CCC'GGG Cuts at: 1872 4306 1872 Size: 2434 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 3763 5083 5984 6246 820 Size: 197 926 543 1277 1320 901 262 1553 Fragments arranged by size: 1553 1320 1277 926 901 543 262 197 SnaBI TAC'GTA Cuts at: 493 3481 4770 493 Size: 2988 1289 2702 Fragments arranged by size: 2988 2702 1289 SpeI A'CTAG_T Cuts at: 152 4019 152 Size: 3867 3112 SphI G_CATG'C Cuts at: 1132 4337 1132 Size: 3205 3774 SspI AAT'ATT Cuts at: 5 52 2524 4903 5 Size: 47 2472 2379 2081 Fragments arranged by size: 2472 2379 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6979 StyI C'CwwG_G Cuts at: 513 1065 2389 2623 2961 3781 4123 4418 513 Size: 552 1324 234 338 820 342 295 3074 Fragments arranged by size: 3074 1324 820 552 342 338 295 234 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5006 5023 5176 5361 5806 325 Size: 1255 3426 17 153 185 445 1498 Fragments arranged by size: 3426 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2446 2466 3722 Size: 274 80 33 51 1443 469 20 1256 Cuts at: 3722 3929 5225 6937 96 Size: 207 1296 1712 138 Fragments arranged by size: 1712 1443 1296 1256 469 274 207 138 80 51 33 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3581 3613 6939 6971 3581 Size: 32 3326 32 3589 Fragments arranged by size: 3589 3326 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6979 XbaI T'CTAG_A Cuts at: 4315 4315 Size: 6979 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2227 4024 2227 Size: 1797 5182 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6979 XmaI C'CCGG_G Cuts at: 1870 4304 1870 Size: 2434 4545 XmnI GAAnn'nnTTC Cuts at: 2499 2796 5108 2499 Size: 297 2312 4370 Fragments arranged by size: 4370 2312 297 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4787 276 Size: 53 83 186 936 3253 2468 Fragments arranged by size: 3253 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI