(Circular) (Six-base) MAPSORT of: pFN21AA1464.seq Check: 6259 from: 1 to: 6565 pFN21AA1464 6565 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2549 2549 Size: 6565 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4375 278 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3908 1937 Size: 1971 4594 Acc65I G'GTAC_C Cuts at: 3886 3886 Size: 6565 AclI AA'CG_TT Cuts at: 1563 4692 5065 1563 Size: 3129 373 3063 Fragments arranged by size: 3129 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3616 4628 6012 6280 1632 Size: 1984 1012 1384 268 1917 Fragments arranged by size: 1984 1917 1384 1012 268 AfeI AGC'GCT Cuts at: 2951 2951 Size: 6565 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6368 AflIII A'CryG_T Cuts at: 1203 5464 6441 1203 Size: 4261 977 1327 Fragments arranged by size: 4261 1327 977 AgeI A'CCGG_T Cuts at: 5396 5396 Size: 6565 AhdI GACnn_n'nnGTC Cuts at: 5294 5294 Size: 6565 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6565 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6565 AlwNI CAG_nnn'CTG Cuts at: 2107 2128 2905 3748 4308 5880 2107 Size: 21 777 843 560 1572 2792 Fragments arranged by size: 2792 1572 843 777 560 21 ApaLI G'TGCA_C Cuts at: 4622 5778 4622 Size: 1156 5409 ApoI r'AATT_y Cuts at: 1493 3598 3874 4100 4252 6361 1493 Size: 2105 276 226 152 2109 1697 Fragments arranged by size: 2109 2105 1697 276 226 152 AseI AT'TA_AT Cuts at: 160 1032 5119 160 Size: 872 4087 1606 Fragments arranged by size: 4087 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2461 3813 3890 1870 Size: 73 83 435 1352 77 4545 Fragments arranged by size: 4545 1352 435 83 77 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3319 3352 3319 Size: 33 6532 BamHI G'GATC_C Cuts at: 3895 4262 3895 Size: 367 6198 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2537 3434 3886 5341 618 Size: 325 217 627 750 897 452 1455 1842 Fragments arranged by size: 1842 1455 897 750 627 452 325 217 BanII G_rGCy'C Cuts at: 1391 2180 3062 3884 1391 Size: 789 882 822 4072 Fragments arranged by size: 4072 882 822 789 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5594 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3163 3197 4756 4790 1903 Size: 34 1226 34 1559 34 3678 Fragments arranged by size: 3678 1559 1226 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2142 4457 5667 2142 Size: 2315 1210 3040 Fragments arranged by size: 3040 2315 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6565 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2925 3672 Size: 107 122 71 631 605 337 916 747 Cuts at: 3672 5176 136 Size: 1504 1525 Fragments arranged by size: 1525 1504 916 747 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6560 6560 Size: 6565 BlpI GC'TnA_GC Cuts at: 2108 3982 2108 Size: 1874 4691 Bme1580I G_kGCm'C Cuts at: 1790 2723 2749 4626 5782 1790 Size: 933 26 1877 1156 2573 Fragments arranged by size: 2573 1877 1156 933 26 BmgBI CAC'GTC Cuts at: 1318 2506 1318 Size: 1188 5377 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3120 3662 4296 Size: 416 53 83 399 24 1703 542 634 Cuts at: 4296 5254 442 Size: 958 1753 Fragments arranged by size: 1753 1703 958 634 542 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6565 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3316 3316 Size: 6565 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2194 2933 2990 3679 5225 1239 Size: 955 739 57 689 1546 2579 Fragments arranged by size: 2579 1546 955 739 689 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2361 2984 4690 5555 5853 2361 Size: 623 1706 865 298 3073 Fragments arranged by size: 3073 1706 865 623 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2210 2933 3344 5228 882 Size: 617 711 723 411 1884 2219 Fragments arranged by size: 2219 1884 723 711 617 411 BsaAI yAC'GTr Cuts at: 493 1204 4356 493 Size: 711 3152 2702 Fragments arranged by size: 3152 2702 711 BsaBI GATnn'nnATC Cuts at: 6559 6559 Size: 6565 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2382 2646 2873 3180 Size: 53 83 186 936 849 264 227 307 Cuts at: 3180 4372 4754 5389 275 Size: 1192 382 635 1451 Fragments arranged by size: 1451 1192 936 849 635 382 307 264 227 186 83 53 BsaWI w'CCGG_w Cuts at: 4998 5396 5670 5817 6228 4998 Size: 398 274 147 411 5335 Fragments arranged by size: 5335 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3080 3110 1178 Size: 30 165 30 1677 30 4633 Fragments arranged by size: 4633 1677 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2047 2449 2978 3223 3238 3241 3244 3247 Size: 861 402 529 245 15 3 3 3 Cuts at: 3247 1186 Size: 4504 Fragments arranged by size: 4504 861 529 402 245 15 3 3 3 BseYI C'CCAG_C Cuts at: 2033 2072 2946 3101 3164 3227 3321 5768 6264 Size: 39 874 155 63 63 94 2447 496 Cuts at: 6264 2033 Size: 2334 Fragments arranged by size: 2447 2334 874 496 155 94 63 63 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3207 1163 Size: 573 1471 4521 Fragments arranged by size: 4521 1471 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4015 4776 5804 1540 Size: 275 186 2014 761 1028 2301 Fragments arranged by size: 2301 2014 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2976 3062 3387 4626 4711 5782 2976 Size: 86 325 1239 85 1071 3759 Fragments arranged by size: 3759 1239 1071 325 86 85 BsmI GAATG_Cn' Cuts at: 2446 4076 4169 2446 Size: 1630 93 4842 Fragments arranged by size: 4842 1630 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2496 2496 Size: 6565 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2180 2723 2749 2976 3062 3387 3884 Size: 399 390 543 26 227 86 325 497 Cuts at: 3884 4626 4711 5782 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 543 497 399 390 325 227 86 85 26 BspEI T'CCGG_A Cuts at: 6228 6228 Size: 6565 BspHI T'CATG_A Cuts at: 4454 6184 4454 Size: 1730 4835 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2302 2549 3462 3756 3920 844 Size: 1458 247 913 294 164 3489 Fragments arranged by size: 3489 1458 913 294 247 164 BsrBI CCG'CTC Cuts at: 1394 1823 4452 1394 Size: 429 2629 3507 Fragments arranged by size: 3507 2629 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3181 5060 5234 65 Size: 1183 1933 1879 174 1396 Fragments arranged by size: 1933 1879 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2112 3006 5209 5396 1700 Size: 110 302 894 2203 187 2869 Fragments arranged by size: 2869 2203 894 302 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6565 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6565 BssSI C'ACGA_G Cuts at: 2798 4625 5637 2798 Size: 1827 1012 3726 Fragments arranged by size: 3726 1827 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2770 794 Size: 1976 4589 BstBI TT'CG_AA Cuts at: 5369 5369 Size: 6565 BstEII G'GTnAC_C Cuts at: 1184 2426 1184 Size: 1242 5323 BstXI CCAn_nnnn'nTGG Cuts at: 2480 2570 2480 Size: 90 6475 BstYI r'GATC_y Cuts at: 1972 2895 3895 4262 4647 4664 6116 6202 6214 Size: 923 1000 367 385 17 1452 86 12 Cuts at: 6214 6560 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1000 923 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2136 2431 2826 513 Size: 552 939 132 295 395 4252 Fragments arranged by size: 4252 939 552 395 295 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5826 Fragments arranged by size: 5826 683 56 BtsI GCAGTG_nn' Cuts at: 2728 2775 4081 4875 4895 2728 Size: 47 1306 794 20 4398 Fragments arranged by size: 4398 1306 794 47 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6565 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2815 2850 3406 3441 529 Size: 35 2251 35 556 35 3653 Fragments arranged by size: 3653 2251 556 35 35 35 DraI TTT'AAA Cuts at: 3869 3869 Size: 6565 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3044 3466 5572 809 Size: 507 1728 422 2106 1802 Fragments arranged by size: 2106 1802 1728 507 422 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2470 2560 2595 Size: 54 1425 50 275 18 640 90 35 Cuts at: 2595 3547 3673 4012 4901 6530 8 Size: 952 126 339 889 1629 43 Fragments arranged by size: 1629 1425 952 889 640 339 275 126 90 54 50 43 35 18 EagI C'GGCC_G Cuts at: 1537 1812 4012 1537 Size: 275 2200 4090 Fragments arranged by size: 4090 2200 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2338 2996 3271 4495 1353 Size: 985 658 275 1224 3423 Fragments arranged by size: 3423 1224 985 658 275 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5152 5526 5672 387 Size: 1064 138 3563 374 146 1280 Fragments arranged by size: 3563 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3060 3060 Size: 6565 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2194 2933 2990 3616 3679 4628 5225 Size: 393 562 739 57 626 63 949 597 Cuts at: 5225 6012 6280 1239 Size: 787 268 1524 Fragments arranged by size: 1524 949 787 739 626 597 562 393 268 63 57 EcoNI CCTnn'n_nnAGG Cuts at: 3782 3782 Size: 6565 EcoO109I rG'GnC_Cy Cuts at: 3451 3451 Size: 6565 EcoRI G'AATT_C Cuts at: 3874 4252 3874 Size: 378 6187 FspI TGC'GCA Cuts at: 5071 5071 Size: 6565 HaeII r_GCGC'y Cuts at: 1678 2073 2119 2128 2953 3729 5712 1678 Size: 395 46 9 825 776 1983 2531 Fragments arranged by size: 2531 1983 825 776 395 46 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2284 2493 2525 2880 Size: 32 135 32 680 32 209 32 355 Cuts at: 2880 2912 5206 5238 5280 5312 1373 Size: 32 2294 32 42 32 2626 Fragments arranged by size: 2626 2294 680 355 209 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2143 2386 2524 3909 4155 669 Size: 988 281 205 243 138 1385 246 3079 Fragments arranged by size: 3079 1385 988 281 246 243 205 138 HindIII A'AGCT_T Cuts at: 748 748 Size: 6565 HpaI GTT'AAC Cuts at: 4155 4155 Size: 6565 KpnI G_GTAC'C Cuts at: 3890 3890 Size: 6565 MfeI C'AATT_G Cuts at: 4164 4164 Size: 6565 MluI A'CGCG_T Cuts at: 6441 6441 Size: 6565 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3321 5679 5863 1305 Size: 2016 2358 184 2007 Fragments arranged by size: 2358 2016 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2472 2562 3675 10 Size: 54 1425 343 640 90 1113 2900 Fragments arranged by size: 2900 1425 1113 640 343 90 54 MslI CAynn'nnrTG Cuts at: 518 1166 2259 2421 2436 3614 3793 4523 4882 Size: 648 1093 162 15 1178 179 730 359 Cuts at: 4882 5041 518 Size: 159 2042 Fragments arranged by size: 2042 1178 1093 730 648 359 179 162 159 15 MspA1I CmG'CkG Cuts at: 1606 2138 2161 3168 3981 4658 5806 6051 1606 Size: 532 23 1007 813 677 1148 245 2120 Fragments arranged by size: 2120 1148 1007 813 677 532 245 23 NaeI GCC'GGC Cuts at: 2114 3008 2114 Size: 894 5671 NcoI C'CATG_G Cuts at: 513 1065 2004 2431 513 Size: 552 939 427 4647 Fragments arranged by size: 4647 939 552 427 NdeI CA'TA_TG Cuts at: 387 387 Size: 6565 NgoMIV G'CCGG_C Cuts at: 2112 3006 2112 Size: 894 5671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6565 NotI GC'GGCC_GC Cuts at: 4012 4012 Size: 6565 NspI r_CATG'y Cuts at: 1132 1229 2695 3142 3415 3923 5468 1132 Size: 97 1466 447 273 508 1545 2229 Fragments arranged by size: 2229 1545 1466 508 447 273 97 PasI CC'CwG_GG Cuts at: 1348 2093 2212 2960 3778 1348 Size: 745 119 748 818 4135 Fragments arranged by size: 4135 818 748 745 119 PciI A'CATG_T Cuts at: 5464 5464 Size: 6565 PflMI CCAn_nnn'nTGG Cuts at: 1108 2905 1108 Size: 1797 4768 PfoI T'CCnGG_A Cuts at: 2171 2171 Size: 6565 PmeI GTTT'AAAC Cuts at: 3869 3869 Size: 6565 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4634 4666 6147 6179 4634 Size: 32 1481 32 5020 Fragments arranged by size: 5020 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3451 3451 Size: 6565 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6565 PsiI TTA'TAA Cuts at: 4135 4135 Size: 6565 PstI C_TGCA'G Cuts at: 830 2625 2778 3459 3624 3917 830 Size: 1795 153 681 165 293 3478 Fragments arranged by size: 3478 1795 681 293 165 153 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6565 PvuII CAG'CTG Cuts at: 3168 3168 Size: 6565 SacI G_AGCT'C Cuts at: 3062 3062 Size: 6565 SacII CC_GC'GG Cuts at: 2139 2139 Size: 6565 SalI G'TCGA_C Cuts at: 1936 3907 1936 Size: 1971 4594 SbfI CC_TGCA'GG Cuts at: 3917 3917 Size: 6565 ScaI AGT'ACT Cuts at: 4813 6525 4813 Size: 1712 4853 SfcI C'TryA_G Cuts at: 826 1045 2621 2774 3169 3455 3620 3913 5048 Size: 219 1576 153 395 286 165 293 1135 Cuts at: 5048 5729 5920 826 Size: 681 191 1471 Fragments arranged by size: 1576 1471 1135 681 395 293 286 219 191 165 153 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6565 SmaI CCC'GGG Cuts at: 1872 2028 3892 1872 Size: 156 1864 4545 Fragments arranged by size: 4545 1864 156 SmlI C'TyrA_G Cuts at: 820 1017 1943 2376 2999 4669 5570 5832 820 Size: 197 926 433 623 1670 901 262 1553 Fragments arranged by size: 1670 1553 926 901 623 433 262 197 SnaBI TAC'GTA Cuts at: 493 4356 493 Size: 3863 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6565 SphI G_CATG'C Cuts at: 1132 3923 1132 Size: 2791 3774 SspI AAT'ATT Cuts at: 5 52 4489 5 Size: 47 4437 2081 Fragments arranged by size: 4437 2081 47 StuI AGG'CCT Cuts at: 1517 2619 3822 1517 Size: 1102 1203 4260 Fragments arranged by size: 4260 1203 1102 StyI C'CwwG_G Cuts at: 513 1065 2004 2181 2431 2569 2709 4004 513 Size: 552 939 177 250 138 140 1295 3074 Fragments arranged by size: 3074 1295 939 552 250 177 140 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2297 2960 3241 4592 4609 4762 4947 Size: 1255 717 663 281 1351 17 153 185 Cuts at: 4947 5392 325 Size: 445 1498 Fragments arranged by size: 1498 1351 1255 717 663 445 281 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4811 6523 96 Size: 274 80 33 51 1443 2834 1712 138 Fragments arranged by size: 2834 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3025 3057 3692 3724 6525 6557 3025 Size: 32 635 32 2801 32 3033 Fragments arranged by size: 3033 2801 635 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6565 XbaI T'CTAG_A Cuts at: 3901 3901 Size: 6565 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2438 2438 Size: 6565 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6565 XmaI C'CCGG_G Cuts at: 1870 2026 3890 1870 Size: 156 1864 4545 Fragments arranged by size: 4545 1864 156 XmnI GAAnn'nnTTC Cuts at: 4694 4694 Size: 6565 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4373 276 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI