Restriction map for
pFN21AA1452
(Circular) (Six-base) MAPSORT of: pFN21AA1452.seq Check: 5725 from: 1 to: 6829
pFN21AA1452 6829 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2634 2634
Size: 6829
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4639 278
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4172 1937
Size: 2235 4594
Acc65I G'GTAC_C
Cuts at: 4104 4150 4104
Size: 46 6783
AclI AA'CG_TT
Cuts at: 1563 4956 5329 1563
Size: 3393 373 3063
Fragments arranged by size:
3393 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3710 3784 4892 6276 6544 1632
Size: 2078 74 1108 1384 268 1917
Fragments arranged by size:
2078 1917 1384 1108 268 74
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6632
AflIII A'CryG_T
Cuts at: 1203 3420 5728 6705 1203
Size: 2217 2308 977 1327
Fragments arranged by size:
2308 2217 1327 977
AgeI A'CCGG_T
Cuts at: 5660 5660
Size: 6829
AhdI GACnn_n'nnGTC
Cuts at: 5558 5558
Size: 6829
AleI CACnn'nnGTG
Cuts at: 1166 3674 1166
Size: 2508 4321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6829
AlwNI CAG_nnn'CTG
Cuts at: 2677 3356 3862 4572 6144 2677
Size: 679 506 710 1572 3362
Fragments arranged by size:
3362 1572 710 679 506
ApaI G_GGCC'C
Cuts at: 2912 2912
Size: 6829
ApaLI G'TGCA_C
Cuts at: 4886 6042 4886
Size: 1156 5673
ApoI r'AATT_y
Cuts at: 1493 3969 4138 4364 4516 6625 1493
Size: 2476 169 226 152 2109 1697
Fragments arranged by size:
2476 2109 1697 226 169 152
AseI AT'TA_AT
Cuts at: 160 1032 5383 160
Size: 872 4351 1606
Fragments arranged by size:
4351 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
AvaI C'yCGr_G
Cuts at: 1870 1943 3611 3681 4154 1870
Size: 73 1668 70 473 4545
Fragments arranged by size:
4545 1668 473 73 70
BamHI G'GATC_C
Cuts at: 3384 4159 4526 3384
Size: 775 367 5687
Fragments arranged by size:
5687 775 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3466 3645 3865 4104 4150
Size: 325 217 627 1679 179 220 239 46
Cuts at: 4150 5605 618
Size: 1455 1842
Fragments arranged by size:
1842 1679 1455 627 325 239 220 217
179 46
BanII G_rGCy'C
Cuts at: 1391 2376 2616 2718 2912 3507 3558 4148 1391
Size: 985 240 102 194 595 51 590 4072
Fragments arranged by size:
4072 985 595 590 240 194 102 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2511 928
Size: 971 612 5246
Fragments arranged by size:
5246 971 612
BbvCI CC'TCA_GC
Cuts at: 3042 3042
Size: 6829
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5020 5054 1903
Size: 34 3083 34 3678
Fragments arranged by size:
3678 3083 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3290 4721 5931 3290
Size: 1431 1210 4188
Fragments arranged by size:
4188 1431 1210
BclI T'GATC_A
Cuts at: 1548 3943 1548
Size: 2395 4434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2474 2907 3361
Size: 107 122 71 631 605 802 433 454
Cuts at: 3361 3613 5440 136
Size: 252 1827 1525
Fragments arranged by size:
1827 1525 802 631 605 454 433 252
122 107 71
BglII A'GATC_T
Cuts at: 4039 6824 4039
Size: 2785 4044
BlpI GC'TnA_GC
Cuts at: 3737 4246 3737
Size: 509 6320
Bme1580I G_kGCm'C
Cuts at: 1790 2687 2912 3629 3648 4890 6046 1790
Size: 897 225 717 19 1242 1156 2573
Fragments arranged by size:
2573 1242 1156 897 717 225 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6829
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2021 2724 2986
Size: 416 53 83 399 24 604 703 262
Cuts at: 2986 4560 5518 442
Size: 1574 958 1753
Fragments arranged by size:
1753 1574 958 703 604 416 399 262
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6829
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4058
Size: 6829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3182 3449 3951 5489 1239
Size: 1943 267 502 1538 2579
Fragments arranged by size:
2579 1943 1538 502 267
Bpu10I CC'TnA_GC
Cuts at: 3042 3042
Size: 6829
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2198 2677 3731 4008 4954 5819 6117 2198
Size: 479 1054 277 946 865 298 2910
Fragments arranged by size:
2910 1054 946 865 479 298 277
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2261 5492 882
Size: 617 762 3231 2219
Fragments arranged by size:
3231 2219 762 617
BsaAI yAC'GTr
Cuts at: 493 1204 2277 4620 493
Size: 711 1073 2343 2702
Fragments arranged by size:
2702 2343 1073 711
BsaBI GATnn'nnATC
Cuts at: 2186 2852 3948 6823 2186
Size: 666 1096 2875 2192
Fragments arranged by size:
2875 2192 1096 666
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2442 4636 5018 5653
Size: 53 83 186 936 909 2194 382 635
Cuts at: 5653 275
Size: 1451
Fragments arranged by size:
2194 1451 936 909 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5262 5660 5934 6081 6492 5262
Size: 398 274 147 411 5599
Fragments arranged by size:
5599 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6604
Fragments arranged by size:
6604 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2624 2783 2786 3515 3530 3539 3548 3563
Size: 1438 159 3 729 15 9 9 15
Cuts at: 3563 3566 1186
Size: 3 4449
Fragments arranged by size:
4449 1438 729 159 15 15 9 9
3 3
BseYI C'CCAG_C
Cuts at: 2414 2759 2795 2817 3096 3195 3408 3572 6032
Size: 345 36 22 279 99 213 164 2460
Cuts at: 6032 6528 2414
Size: 496 2715
Fragments arranged by size:
2715 2460 496 345 279 213 164 99
36 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2337 2594 3749 1163
Size: 573 601 257 1155 4243
Fragments arranged by size:
4243 1155 601 573 257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2543 4279 5040 6068 1540
Size: 275 186 542 1736 761 1028 2301
Fragments arranged by size:
2301 1736 1028 761 542 275 186
BsiHKAI G_wGCw'C
Cuts at: 2376 2718 3051 3168 3732 4890 4975 6046 2376
Size: 342 333 117 564 1158 85 1071 3159
Fragments arranged by size:
3159 1158 1071 564 342 333 117 85
BsmI GAATG_Cn'
Cuts at: 4340 4433 4340
Size: 93 6736
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2376 2616 2687 2718 2912 3051 3168
Size: 399 586 240 71 31 194 139 117
Cuts at: 3168 3507 3558 3629 3648 3732 4148 4890 4975
Size: 339 51 71 19 84 416 742 85
Cuts at: 4975 6046 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 586 416 399 339 240
194 139 117 85 84 71 71 51
31 19
BspEI T'CCGG_A
Cuts at: 6492 6492
Size: 6829
BspHI T'CATG_A
Cuts at: 4718 6448 4718
Size: 1730 5099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2634 2685 4184 844
Size: 1790 51 1499 3489
Fragments arranged by size:
3489 1790 1499 51
BsrBI CCG'CTC
Cuts at: 1394 1823 3433 4716 1394
Size: 429 1610 1283 3507
Fragments arranged by size:
3507 1610 1283 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5324 5498 65
Size: 1183 4076 174 1396
Fragments arranged by size:
4076 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3845 5473 5660 1700
Size: 110 2035 1628 187 2869
Fragments arranged by size:
2869 2035 1628 187 110
BsrGI T'GTAC_A
Cuts at: 96 2032 96
Size: 1936 4893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6829
BssSI C'ACGA_G
Cuts at: 4889 5901 4889
Size: 1012 5817
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3265 794
Size: 2471 4358
BstBI TT'CG_AA
Cuts at: 5633 5633
Size: 6829
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6829
BstXI CCAn_nnnn'nTGG
Cuts at: 2065 2065
Size: 6829
BstYI r'GATC_y
Cuts at: 1972 2853 3384 4039 4159 4526 4911 4928 6380
Size: 881 531 655 120 367 385 17 1452
Cuts at: 6380 6466 6478 6824 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 881 655 531 385 367 346
120 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2642 2642
Size: 6829
BtgI C'CryG_G
Cuts at: 513 1065 2004 2925 3174 3558 513
Size: 552 939 921 249 384 3784
Fragments arranged by size:
3784 939 921 552 384 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2972 487
Size: 683 56 1746 4344
Fragments arranged by size:
4344 1746 683 56
BtsI GCAGTG_nn'
Cuts at: 4345 5139 5159 4345
Size: 794 20 6015
Fragments arranged by size:
6015 794 20
ClaI AT'CG_AT
Cuts at: 1554 2043 2919 1554
Size: 489 876 5464
Fragments arranged by size:
5464 876 489
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2682 2717 529
Size: 35 2118 35 4641
Fragments arranged by size:
4641 2118 35 35
DraI TTT'AAA
Cuts at: 4133 4133
Size: 6829
DraIII CAC_nnn'GTG
Cuts at: 2732 2732
Size: 6829
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4123 5836 809
Size: 507 2807 1713 1802
Fragments arranged by size:
2807 1802 1713 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2055 2184
Size: 54 1425 50 275 18 177 48 129
Cuts at: 2184 2466 2540 2838 3171 3594 3771 3787 3840
Size: 282 74 298 333 423 177 16 53
Cuts at: 3840 4276 5165 6794 8
Size: 436 889 1629 43
Fragments arranged by size:
1629 1425 889 436 423 333 298 282
275 177 177 129 74 54 53 50
48 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2540 4276 1537
Size: 275 728 1736 4090
Fragments arranged by size:
4090 1736 728 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 2320 2491 2981 3761 3803 4759 1353
Size: 657 310 171 490 780 42 956 3423
Fragments arranged by size:
3423 956 780 657 490 310 171 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2084 2516 3463 3560 5416 5790
Size: 1064 138 495 432 947 97 1856 374
Cuts at: 5790 5936 387
Size: 146 1280
Fragments arranged by size:
1856 1280 1064 947 495 432 374 146
138 97
EcoICRI GAG'CTC
Cuts at: 2374 2716 2374
Size: 342 6487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3182 3449 3710 3784 3951 4892 5489
Size: 393 1550 267 261 74 167 941 597
Cuts at: 5489 6276 6544 1239
Size: 787 268 1524
Fragments arranged by size:
1550 1524 941 787 597 393 268 267
261 167 74
EcoNI CCTnn'n_nnAGG
Cuts at: 2432 2432
Size: 6829
EcoO109I rG'GnC_Cy
Cuts at: 2253 2909 3353 2253
Size: 656 444 5729
Fragments arranged by size:
5729 656 444
EcoRI G'AATT_C
Cuts at: 4138 4516 4138
Size: 378 6451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2323 3772 2323
Size: 1449 5380
FspI TGC'GCA
Cuts at: 2825 5335 2825
Size: 2510 4319
HaeII r_GCGC'y
Cuts at: 1678 2569 3754 5976 1678
Size: 891 1185 2222 2531
Fragments arranged by size:
2531 2222 1185 891
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2635 2639 2667 2671 3332
Size: 32 135 32 1063 4 28 4 661
Cuts at: 3332 3364 3997 4029 5470 5502 5544 5576 1373
Size: 32 633 32 1441 32 42 32 2626
Fragments arranged by size:
2626 1441 1063 661 633 135 42 32
32 32 32 32 32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4020 4173 4419 669
Size: 988 281 2082 153 246 3079
Fragments arranged by size:
3079 2082 988 281 246 153
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6829
HpaI GTT'AAC
Cuts at: 4419 4419
Size: 6829
KpnI G_GTAC'C
Cuts at: 4108 4154 4108
Size: 46 6783
MfeI C'AATT_G
Cuts at: 4428 4428
Size: 6829
MluI A'CGCG_T
Cuts at: 3420 6705 3420
Size: 3285 3544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5943 6127 1305
Size: 4638 184 2007
Fragments arranged by size:
4638 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2057 2186 3596 3773
Size: 54 1425 343 177 48 129 1410 177
Cuts at: 3773 10
Size: 3066
Fragments arranged by size:
3066 1425 1410 343 177 177 129 54
48
MslI CAynn'nnrTG
Cuts at: 518 1166 2634 3173 3243 3674 4787 5146 5305
Size: 648 1468 539 70 431 1113 359 159
Cuts at: 5305 518
Size: 2042
Fragments arranged by size:
2042 1468 1113 648 539 431 359 159
70
MspA1I CmG'CkG
Cuts at: 1606 2351 2407 2821 3030 3096 3256 3382 3445
Size: 745 56 414 209 66 160 126 63
Cuts at: 3445 3460 3576 3804 4245 4922 6070 6315 1606
Size: 15 116 228 441 677 1148 245 2120
Fragments arranged by size:
2120 1148 745 677 441 414 245 228
209 160 126 116 66 63 56 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 3558 513
Size: 552 939 1554 3784
Fragments arranged by size:
3784 1554 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6829
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6829
NotI GC'GGCC_GC
Cuts at: 4276 4276
Size: 6829
NspI r_CATG'y
Cuts at: 1132 1229 3268 3823 4102 4187 5732 1132
Size: 97 2039 555 279 85 1545 2229
Fragments arranged by size:
2229 2039 1545 555 279 97 85
PasI CC'CwG_GG
Cuts at: 1348 2616 3655 1348
Size: 1268 1039 4522
Fragments arranged by size:
4522 1268 1039
PciI A'CATG_T
Cuts at: 5728 5728
Size: 6829
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2953 1108
Size: 1845 4984
PfoI T'CCnGG_A
Cuts at: 3159 3159
Size: 6829
PmeI GTTT'AAAC
Cuts at: 4133 4133
Size: 6829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4898 4930 6411 6443 4898
Size: 32 1481 32 5284
Fragments arranged by size:
5284 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6829
PsiI TTA'TAA
Cuts at: 4399 4399
Size: 6829
PspOMI G'GGCC_C
Cuts at: 2908 2908
Size: 6829
PstI C_TGCA'G
Cuts at: 830 2406 2595 3056 3444 4181 830
Size: 1576 189 461 388 737 3478
Fragments arranged by size:
3478 1576 737 461 388 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6829
PvuII CAG'CTG
Cuts at: 2407 2821 3460 2407
Size: 414 639 5776
Fragments arranged by size:
5776 639 414
RsrII CG'GwC_CG
Cuts at: 3814 3814
Size: 6829
SacI G_AGCT'C
Cuts at: 2376 2718 2376
Size: 342 6487
SalI G'TCGA_C
Cuts at: 1936 4171 1936
Size: 2235 4594
SapI GCTCTTCn'nnn_
Cuts at: 2320 2320
Size: 6829
SbfI CC_TGCA'GG
Cuts at: 4181 4181
Size: 6829
ScaI AGT'ACT
Cuts at: 4068 5077 6789 4068
Size: 1009 1712 4108
Fragments arranged by size:
4108 1712 1009
SfcI C'TryA_G
Cuts at: 826 1045 2359 2402 2591 3052 3440 3824 4177
Size: 219 1314 43 189 461 388 384 353
Cuts at: 4177 5312 5993 6184 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1314 1135 681 461 388 384 353
219 191 189 43
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6829
SmaI CCC'GGG
Cuts at: 1872 4156 1872
Size: 2284 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2177 2692 3746 4023 4933 5834
Size: 197 926 234 515 1054 277 910 901
Cuts at: 5834 6096 820
Size: 262 1553
Fragments arranged by size:
1553 1054 926 910 901 515 277 262
234 197
SnaBI TAC'GTA
Cuts at: 493 4620 493
Size: 4127 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6829
SphI G_CATG'C
Cuts at: 1132 3823 4187 1132
Size: 2691 364 3774
Fragments arranged by size:
3774 2691 364
SspI AAT'ATT
Cuts at: 5 52 4753 5
Size: 47 4701 2081
Fragments arranged by size:
4701 2081 47
StuI AGG'CCT
Cuts at: 1517 3695 1517
Size: 2178 4651
StyI C'CwwG_G
Cuts at: 513 1065 2004 2968 3006 3402 3558 4268 513
Size: 552 939 964 38 396 156 710 3074
Fragments arranged by size:
3074 964 939 710 552 396 156 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2743 2773 2968 3088 4856 4873 5026
Size: 1255 1163 30 195 120 1768 17 153
Cuts at: 5026 5211 5656 325
Size: 185 445 1498
Fragments arranged by size:
1768 1498 1255 1163 445 195 185 153
120 30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2032 2049 4066
Size: 274 80 33 51 1443 55 17 2017
Cuts at: 4066 4120 5075 6787 96
Size: 54 955 1712 138
Fragments arranged by size:
2017 1712 1443 955 274 138 80 55
54 51 33 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6789 6821 6789
Size: 32 6797
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6829
XbaI T'CTAG_A
Cuts at: 4165 4165
Size: 6829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2923 3673 2923
Size: 750 6079
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6829
XmaI C'CCGG_G
Cuts at: 1870 4154 1870
Size: 2284 4545
XmnI GAAnn'nnTTC
Cuts at: 3155 3695 4958 3155
Size: 540 1263 5026
Fragments arranged by size:
5026 1263 540
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4637 276
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
SacII SanDI SexAI SfiI SfoI SrfI SwaI