(Circular) (Six-base) MAPSORT of: pFN21AA1434.seq Check: 1249 from: 1 to: 6721 pFN21AA1434 6721 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4531 278 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4064 1937 Size: 2127 4594 Acc65I G'GTAC_C Cuts at: 4042 4042 Size: 6721 AclI AA'CG_TT Cuts at: 1563 4848 5221 1563 Size: 3285 373 3063 Fragments arranged by size: 3285 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3711 3914 4784 6168 6436 1632 Size: 2079 203 870 1384 268 1917 Fragments arranged by size: 2079 1917 1384 870 268 203 AflII C'TTAA_G Cuts at: 820 1017 3326 3914 820 Size: 197 2309 588 3627 Fragments arranged by size: 3627 2309 588 197 AflIII A'CryG_T Cuts at: 1203 2387 2755 5620 6597 1203 Size: 1184 368 2865 977 1327 Fragments arranged by size: 2865 1327 1184 977 368 AgeI A'CCGG_T Cuts at: 5552 5552 Size: 6721 AhdI GACnn_n'nnGTC Cuts at: 5450 5450 Size: 6721 AleI CACnn'nnGTG Cuts at: 1166 2355 3239 1166 Size: 1189 884 4648 Fragments arranged by size: 4648 1189 884 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3544 1184 Size: 2360 4361 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2178 2210 2178 Size: 32 6689 AlwNI CAG_nnn'CTG Cuts at: 4464 6036 4464 Size: 1572 5149 ApaLI G'TGCA_C Cuts at: 4778 5934 4778 Size: 1156 5565 ApoI r'AATT_y Cuts at: 1493 2814 2842 3077 3155 3186 3292 3481 4030 Size: 1321 28 235 78 31 106 189 549 Cuts at: 4030 4256 4408 6517 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1321 549 235 226 189 152 106 78 31 28 AseI AT'TA_AT Cuts at: 160 1032 5275 160 Size: 872 4243 1606 Fragments arranged by size: 4243 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 AvaI C'yCGr_G Cuts at: 1870 1943 4046 1870 Size: 73 2103 4545 Fragments arranged by size: 4545 2103 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2896 2929 2896 Size: 33 6688 BamHI G'GATC_C Cuts at: 4051 4418 4051 Size: 367 6354 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4042 5497 618 Size: 325 217 627 2255 1455 1842 Fragments arranged by size: 2255 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4040 1391 Size: 2649 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3699 928 Size: 971 1800 3950 Fragments arranged by size: 3950 1800 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2621 2655 4912 4946 1903 Size: 34 684 34 2257 34 3678 Fragments arranged by size: 3678 2257 684 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2897 4613 5823 2897 Size: 1716 1210 3795 Fragments arranged by size: 3795 1716 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6721 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3008 5332 136 Size: 107 122 71 631 605 1336 2324 1525 Fragments arranged by size: 2324 1525 1336 631 605 122 107 71 BglII A'GATC_T Cuts at: 2713 3620 6716 2713 Size: 907 3096 2718 Fragments arranged by size: 3096 2718 907 BlpI GC'TnA_GC Cuts at: 4138 4138 Size: 6721 Bme1580I G_kGCm'C Cuts at: 1790 3111 4782 5938 1790 Size: 1321 1671 1156 2573 Fragments arranged by size: 2573 1671 1321 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6721 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3680 4452 5410 Size: 416 53 83 399 24 2263 772 958 Cuts at: 5410 442 Size: 1753 Fragments arranged by size: 2263 1753 958 772 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6721 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 3203 5381 1239 Size: 1283 681 2178 2579 Fragments arranged by size: 2579 2178 1283 681 Bpu10I CC'TnA_GC Cuts at: 3735 3735 Size: 6721 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3564 4846 5711 6009 3564 Size: 1282 865 298 4276 Fragments arranged by size: 4276 1282 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5384 882 Size: 617 3885 2219 Fragments arranged by size: 3885 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2728 4512 493 Size: 711 1524 1784 2702 Fragments arranged by size: 2702 1784 1524 711 BsaBI GATnn'nnATC Cuts at: 3165 6715 3165 Size: 3550 3171 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4528 4910 5545 275 Size: 53 83 186 936 2995 382 635 1451 Fragments arranged by size: 2995 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5154 5552 5826 5973 6384 5154 Size: 398 274 147 411 5491 Fragments arranged by size: 5491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2178 2208 1178 Size: 30 165 30 775 30 5691 Fragments arranged by size: 5691 775 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2199 3508 1186 Size: 1013 1309 4399 Fragments arranged by size: 4399 1309 1013 BseYI C'CCAG_C Cuts at: 3003 3756 5924 6420 3003 Size: 753 2168 496 3304 Fragments arranged by size: 3304 2168 753 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2617 3000 3084 3231 1163 Size: 573 881 383 84 147 4653 Fragments arranged by size: 4653 881 573 383 147 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4171 4932 5960 1540 Size: 275 186 2170 761 1028 2301 Fragments arranged by size: 2301 2170 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2727 4782 4867 5938 2727 Size: 2055 85 1071 3510 Fragments arranged by size: 3510 2055 1071 85 BsmI GAATG_Cn' Cuts at: 2602 4021 4232 4325 2602 Size: 1419 211 93 4998 Fragments arranged by size: 4998 1419 211 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2727 3111 4040 4782 4867 5938 1391 Size: 399 937 384 929 742 85 1071 2174 Fragments arranged by size: 2174 1071 937 929 742 399 384 85 BspEI T'CCGG_A Cuts at: 6384 6384 Size: 6721 BspHI T'CATG_A Cuts at: 2832 3121 4610 6340 2832 Size: 289 1489 1730 3213 Fragments arranged by size: 3213 1730 1489 289 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4076 844 Size: 3232 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4608 1394 Size: 429 2785 3507 Fragments arranged by size: 3507 2785 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2787 3636 3648 5216 5390 65 Size: 1183 1539 849 12 1568 174 1396 Fragments arranged by size: 1568 1539 1396 1183 849 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 5365 5552 1700 Size: 110 3555 187 2869 Fragments arranged by size: 3555 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3682 96 Size: 3586 3135 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6721 BssSI C'ACGA_G Cuts at: 4781 5793 4781 Size: 1012 5709 BstAPI GCAn_nnn'nTGC Cuts at: 794 3904 794 Size: 3110 3611 BstBI TT'CG_AA Cuts at: 2706 5525 2706 Size: 2819 3902 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6721 BstXI CCAn_nnnn'nTGG Cuts at: 2560 2560 Size: 6721 BstYI r'GATC_y Cuts at: 1972 2713 3384 3620 4051 4418 4803 4820 6272 Size: 741 671 236 431 367 385 17 1452 Cuts at: 6272 6358 6370 6716 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 741 671 431 385 367 346 236 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3111 513 Size: 552 2046 4123 Fragments arranged by size: 4123 2046 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5982 Fragments arranged by size: 5982 683 56 BtsI GCAGTG_nn' Cuts at: 4237 5031 5051 4237 Size: 794 20 5907 Fragments arranged by size: 5907 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6721 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6686 DraI TTT'AAA Cuts at: 3465 4025 3465 Size: 560 6161 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5728 809 Size: 507 4412 1802 Fragments arranged by size: 4412 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2968 4168 5057 Size: 54 1425 50 275 18 1138 1200 889 Cuts at: 5057 6686 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1200 1138 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4168 1537 Size: 275 2356 4090 Fragments arranged by size: 4090 2356 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2829 3485 4651 1353 Size: 774 702 656 1166 3423 Fragments arranged by size: 3423 1166 774 702 656 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5308 5682 5828 387 Size: 1064 138 3719 374 146 1280 Fragments arranged by size: 3719 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 3203 3711 3914 4784 5381 6168 Size: 393 890 681 508 203 870 597 787 Cuts at: 6168 6436 1239 Size: 268 1524 Fragments arranged by size: 1524 890 870 787 681 597 508 393 268 203 EcoNI CCTnn'n_nnAGG Cuts at: 3095 3896 3095 Size: 801 5920 EcoRI G'AATT_C Cuts at: 2814 4030 4408 2814 Size: 1216 378 5127 Fragments arranged by size: 5127 1216 378 EcoRV GAT'ATC Cuts at: 2568 3979 2568 Size: 1411 5310 FspI TGC'GCA Cuts at: 5227 5227 Size: 6721 HaeII r_GCGC'y Cuts at: 1678 5868 1678 Size: 4190 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2510 2542 2906 Size: 32 135 32 427 32 479 32 364 Cuts at: 2906 2938 3605 3637 5362 5394 5436 5468 1373 Size: 32 667 32 1725 32 42 32 2626 Fragments arranged by size: 2626 1725 667 479 427 364 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2875 4065 4311 669 Size: 988 281 937 1190 246 3079 Fragments arranged by size: 3079 1190 988 937 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6721 HpaI GTT'AAC Cuts at: 4311 4311 Size: 6721 KpnI G_GTAC'C Cuts at: 4046 4046 Size: 6721 MfeI C'AATT_G Cuts at: 3881 4320 3881 Size: 439 6282 MluI A'CGCG_T Cuts at: 6597 6597 Size: 6721 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2624 5835 6019 1305 Size: 1319 3211 184 2007 Fragments arranged by size: 3211 2007 1319 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2970 10 Size: 54 1425 343 1138 3761 Fragments arranged by size: 3761 1425 1138 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2355 2976 3239 3641 3961 3987 4679 Size: 648 1189 621 263 402 320 26 692 Cuts at: 4679 5038 5197 518 Size: 359 159 2042 Fragments arranged by size: 2042 1189 692 648 621 402 359 320 263 159 26 MspA1I CmG'CkG Cuts at: 1606 3007 4137 4814 5962 6207 1606 Size: 1401 1130 677 1148 245 2120 Fragments arranged by size: 2120 1401 1148 1130 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6169 NdeI CA'TA_TG Cuts at: 387 387 Size: 6721 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6721 NotI GC'GGCC_GC Cuts at: 4168 4168 Size: 6721 NspI r_CATG'y Cuts at: 1132 1229 2155 2391 2759 4079 5624 1132 Size: 97 926 236 368 1320 1545 2229 Fragments arranged by size: 2229 1545 1320 926 368 236 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6721 PciI A'CATG_T Cuts at: 2387 2755 5620 2387 Size: 368 2865 3488 Fragments arranged by size: 3488 2865 368 PflMI CCAn_nnn'nTGG Cuts at: 1108 2940 1108 Size: 1832 4889 PfoI T'CCnGG_A Cuts at: 2749 2846 2749 Size: 97 6624 PmeI GTTT'AAAC Cuts at: 4025 4025 Size: 6721 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2178 2210 4790 4822 6303 6335 2178 Size: 32 2580 32 1481 32 2564 Fragments arranged by size: 2580 2564 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6721 PsiI TTA'TAA Cuts at: 4291 4291 Size: 6721 PstI C_TGCA'G Cuts at: 830 4073 830 Size: 3243 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6721 PvuII CAG'CTG Cuts at: 3007 3007 Size: 6721 SalI G'TCGA_C Cuts at: 1936 4063 1936 Size: 2127 4594 SapI GCTCTTCn'nnn_ Cuts at: 2127 2127 Size: 6721 SbfI CC_TGCA'GG Cuts at: 4073 4073 Size: 6721 ScaI AGT'ACT Cuts at: 4969 6681 4969 Size: 1712 5009 SfcI C'TryA_G Cuts at: 826 1045 4069 5204 5885 6076 826 Size: 219 3024 1135 681 191 1471 Fragments arranged by size: 3024 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6721 SmaI CCC'GGG Cuts at: 1872 4048 1872 Size: 2176 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3326 3579 3914 4825 5726 5988 Size: 197 926 1383 253 335 911 901 262 Cuts at: 5988 820 Size: 1553 Fragments arranged by size: 1553 1383 926 911 901 335 262 253 197 SnaBI TAC'GTA Cuts at: 493 4512 493 Size: 4019 2702 SpeI A'CTAG_T Cuts at: 152 3742 152 Size: 3590 3131 SphI G_CATG'C Cuts at: 1132 2155 4079 1132 Size: 1023 1924 3774 Fragments arranged by size: 3774 1924 1023 SspI AAT'ATT Cuts at: 5 52 4645 5 Size: 47 4593 2081 Fragments arranged by size: 4593 2081 47 StuI AGG'CCT Cuts at: 1517 2499 1517 Size: 982 5739 StyI C'CwwG_G Cuts at: 513 1065 4160 513 Size: 552 3095 3074 Fragments arranged by size: 3095 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4748 4765 4918 5103 5548 325 Size: 1255 3168 17 153 185 445 1498 Fragments arranged by size: 3168 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2173 2536 2652 Size: 274 80 33 51 1443 196 363 116 Cuts at: 2652 3682 4967 6679 96 Size: 1030 1285 1712 138 Fragments arranged by size: 1712 1443 1285 1030 363 274 196 138 116 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2534 2566 3392 3424 6681 6713 2534 Size: 32 826 32 3257 32 2542 Fragments arranged by size: 3257 2542 826 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6721 XbaI T'CTAG_A Cuts at: 2468 4057 2468 Size: 1589 5132 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3398 3398 Size: 6721 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6721 XmaI C'CCGG_G Cuts at: 1870 4046 1870 Size: 2176 4545 XmnI GAAnn'nnTTC Cuts at: 4850 4850 Size: 6721 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4529 276 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI