(Circular) (Six-base) MAPSORT of: pFN21AA1404.seq Check: 5492 from: 1 to: 10459 pFN21AA1404 10459 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8269 278 Size: 53 83 186 936 6733 2468 Fragments arranged by size: 6733 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3285 7802 1937 Size: 1348 4517 4594 Fragments arranged by size: 4594 4517 1348 Acc65I G'GTAC_C Cuts at: 7780 7780 Size: 10459 AclI AA'CG_TT Cuts at: 1563 8586 8959 1563 Size: 7023 373 3063 Fragments arranged by size: 7023 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3335 3681 3812 4091 7062 8522 9906 10174 Size: 1703 346 131 279 2971 1460 1384 268 Cuts at: 10174 1632 Size: 1917 Fragments arranged by size: 2971 1917 1703 1460 1384 346 279 268 131 AfeI AGC'GCT Cuts at: 7238 7238 Size: 10459 AflII C'TTAA_G Cuts at: 820 1017 5297 6995 820 Size: 197 4280 1698 4284 Fragments arranged by size: 4284 4280 1698 197 AflIII A'CryG_T Cuts at: 1203 5127 7397 7607 9358 10335 1203 Size: 3924 2270 210 1751 977 1327 Fragments arranged by size: 3924 2270 1751 1327 977 210 AgeI A'CCGG_T Cuts at: 5997 6241 9290 5997 Size: 244 3049 7166 Fragments arranged by size: 7166 3049 244 AhdI GACnn_n'nnGTC Cuts at: 9188 9188 Size: 10459 AleI CACnn'nnGTG Cuts at: 1166 2966 6171 6424 1166 Size: 1800 3205 253 5201 Fragments arranged by size: 5201 3205 1800 253 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5531 6422 1184 Size: 4347 891 5221 Fragments arranged by size: 5221 4347 891 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5352 5384 6069 6101 5352 Size: 32 685 32 9710 Fragments arranged by size: 9710 685 32 32 AlwNI CAG_nnn'CTG Cuts at: 3202 3704 4000 4249 4409 4637 4967 5110 5275 Size: 502 296 249 160 228 330 143 165 Cuts at: 5275 5713 8202 9774 3202 Size: 438 2489 1572 3887 Fragments arranged by size: 3887 2489 1572 502 438 330 296 249 228 165 160 143 ApaLI G'TGCA_C Cuts at: 3038 4216 6571 8516 9672 3038 Size: 1178 2355 1945 1156 3825 Fragments arranged by size: 3825 2355 1945 1178 1156 ApoI r'AATT_y Cuts at: 1493 2044 2648 3334 3710 4109 7768 7994 8146 Size: 551 604 686 376 399 3659 226 152 Cuts at: 8146 10255 1493 Size: 2109 1697 Fragments arranged by size: 3659 2109 1697 686 604 551 399 376 226 152 AseI AT'TA_AT Cuts at: 160 1032 3750 9013 160 Size: 872 2718 5263 1606 Fragments arranged by size: 5263 2718 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10459 AvaI C'yCGr_G Cuts at: 1870 1943 5670 7784 1870 Size: 73 3727 2114 4545 Fragments arranged by size: 4545 3727 2114 73 AvrII C'CTAG_G Cuts at: 2165 2165 Size: 10459 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4138 4171 4808 4841 4853 4886 5808 5841 4138 Size: 33 637 33 12 33 922 33 8756 Fragments arranged by size: 8756 922 637 33 33 33 33 12 BamHI G'GATC_C Cuts at: 7789 8156 7789 Size: 367 10092 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4773 5722 5994 6046 6874 Size: 325 217 627 2986 949 272 52 828 Cuts at: 6874 7780 9235 618 Size: 906 1455 1842 Fragments arranged by size: 2986 1842 1455 949 906 828 627 325 272 217 52 BanII G_rGCy'C Cuts at: 1391 2523 2562 6594 6962 7693 7778 1391 Size: 1132 39 4032 368 731 85 4072 Fragments arranged by size: 4072 4032 1132 731 368 85 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2023 2450 2767 2991 3198 4031 4920 Size: 971 124 427 317 224 207 833 889 Cuts at: 4920 5316 6777 7050 928 Size: 396 1461 273 4337 Fragments arranged by size: 4337 1461 971 889 833 427 396 317 273 224 207 124 BbvCI CC'TCA_GC Cuts at: 4094 4853 4094 Size: 759 9700 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3080 3114 6591 6625 8650 8684 1903 Size: 34 1143 34 3477 34 2025 34 3678 Fragments arranged by size: 3678 3477 2025 1143 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2733 2947 3106 5836 8351 9561 2733 Size: 214 159 2730 2515 1210 3631 Fragments arranged by size: 3631 2730 2515 1210 214 159 BclI T'GATC_A Cuts at: 1548 2688 1548 Size: 1140 9319 BfrBI ATG'CAT Cuts at: 3369 5958 6441 7171 3369 Size: 2589 483 730 6657 Fragments arranged by size: 6657 2589 730 483 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4546 7557 9070 Size: 107 122 71 631 605 2874 3011 1513 Cuts at: 9070 136 Size: 1525 Fragments arranged by size: 3011 2874 1525 1513 631 605 122 107 71 BglII A'GATC_T Cuts at: 3430 10454 3430 Size: 7024 3435 BlpI GC'TnA_GC Cuts at: 6961 7876 6961 Size: 915 9544 Bme1580I G_kGCm'C Cuts at: 1790 2942 3042 4220 5234 5727 5997 6051 6156 Size: 1152 100 1178 1014 493 270 54 105 Cuts at: 6156 6234 6455 6575 6879 7607 8520 9676 1790 Size: 78 221 120 304 728 913 1156 2573 Fragments arranged by size: 2573 1178 1156 1152 1014 913 728 493 304 270 221 120 105 100 78 54 BmgBI CAC'GTC Cuts at: 1318 3556 1318 Size: 2238 8221 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2444 2636 3714 Size: 416 53 83 399 24 1027 192 1078 Cuts at: 3714 4285 4635 5112 8190 9148 442 Size: 571 350 477 3078 958 1753 Fragments arranged by size: 3078 1753 1078 1027 958 571 477 416 399 350 192 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3488 1056 Size: 2432 8027 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2037 3640 6148 2037 Size: 1603 2508 6348 Fragments arranged by size: 6348 2508 1603 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2741 3554 3929 4232 4373 4383 4892 5297 Size: 1502 813 375 303 141 10 509 405 Cuts at: 5297 7028 9119 1239 Size: 1731 2091 2579 Fragments arranged by size: 2579 2091 1731 1502 813 509 405 375 303 141 10 Bpu10I CC'TnA_GC Cuts at: 4094 4853 4094 Size: 759 9700 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2576 5060 5177 6206 7261 7409 8584 9449 9747 Size: 2484 117 1029 1055 148 1175 865 298 Cuts at: 9747 2576 Size: 3288 Fragments arranged by size: 3288 2484 1175 1055 1029 865 298 148 117 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2277 6665 9122 882 Size: 617 778 4388 2457 2219 Fragments arranged by size: 4388 2457 2219 778 617 BsaAI yAC'GTr Cuts at: 493 1204 7608 8250 493 Size: 711 6404 642 2702 Fragments arranged by size: 6404 2702 711 642 BsaBI GATnn'nnATC Cuts at: 4475 5468 6935 10453 4475 Size: 993 1467 3518 4481 Fragments arranged by size: 4481 3518 1467 993 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5538 8266 8648 9283 Size: 53 83 186 936 4005 2728 382 635 Cuts at: 9283 275 Size: 1451 Fragments arranged by size: 4005 2728 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5997 6241 8892 9290 9564 9711 10122 5997 Size: 244 2651 398 274 147 411 6334 Fragments arranged by size: 6334 2651 411 398 274 244 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2732 2762 3629 3659 4045 Size: 30 165 30 1329 30 867 30 386 Cuts at: 4045 4075 1178 Size: 30 7562 Fragments arranged by size: 7562 1329 867 386 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2151 2154 2744 4454 4469 4472 4535 Size: 838 127 3 590 1710 15 3 63 Cuts at: 4535 5582 7013 7754 1186 Size: 1047 1431 741 3891 Fragments arranged by size: 3891 1710 1431 1047 838 741 590 127 63 15 3 3 BseYI C'CCAG_C Cuts at: 4269 4542 4671 7692 9662 10158 4269 Size: 273 129 3021 1970 496 4570 Fragments arranged by size: 4570 3021 1970 496 273 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2813 2990 3314 4322 4904 5893 6209 Size: 573 1077 177 324 1008 582 989 316 Cuts at: 6209 6782 7451 7523 1163 Size: 573 669 72 4099 Fragments arranged by size: 4099 1077 1008 989 669 582 573 573 324 316 177 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7909 8670 9698 1540 Size: 275 186 5908 761 1028 2301 Fragments arranged by size: 5908 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2523 2562 2733 3042 4220 6575 8520 8605 9676 Size: 39 171 309 1178 2355 1945 85 1071 Cuts at: 9676 2523 Size: 3306 Fragments arranged by size: 3306 2355 1945 1178 1071 309 171 85 39 BsmI GAATG_Cn' Cuts at: 4339 7970 8063 4339 Size: 3631 93 6735 Fragments arranged by size: 6735 3631 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2523 2562 2733 2942 3042 4220 5234 Size: 399 733 39 171 209 100 1178 1014 Cuts at: 5234 5727 5997 6051 6156 6234 6455 6575 6594 Size: 493 270 54 105 78 221 120 19 Cuts at: 6594 6879 6962 7607 7693 7778 8520 8605 9676 Size: 285 83 645 86 85 742 85 1071 Cuts at: 9676 1391 Size: 2174 Fragments arranged by size: 2174 1178 1071 1014 742 733 645 493 399 285 270 221 209 171 120 105 100 86 85 85 83 78 54 39 19 BspEI T'CCGG_A Cuts at: 10122 10122 Size: 10459 BspHI T'CATG_A Cuts at: 4158 4192 4287 8348 10078 4158 Size: 34 95 4061 1730 4539 Fragments arranged by size: 4539 4061 1730 95 34 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4257 4926 5460 7814 844 Size: 3413 669 534 2354 3489 Fragments arranged by size: 3489 3413 2354 669 534 BsrBI CCG'CTC Cuts at: 1394 1823 8346 1394 Size: 429 6523 3507 Fragments arranged by size: 6523 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5052 7561 8954 9128 65 Size: 1183 3804 2509 1393 174 1396 Fragments arranged by size: 3804 2509 1396 1393 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5997 6241 9103 9290 1700 Size: 110 4187 244 2862 187 2869 Fragments arranged by size: 4187 2869 2862 244 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10459 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10459 BssSI C'ACGA_G Cuts at: 7530 8519 9531 7530 Size: 989 1012 8458 Fragments arranged by size: 8458 1012 989 BstAPI GCAn_nnn'nTGC Cuts at: 794 5073 794 Size: 4279 6180 BstBI TT'CG_AA Cuts at: 6363 9263 6363 Size: 2900 7559 BstEII G'GTnAC_C Cuts at: 1184 6208 6712 1184 Size: 5024 504 4931 Fragments arranged by size: 5024 4931 504 BstXI CCAn_nnnn'nTGG Cuts at: 4210 4351 6173 4210 Size: 141 1822 8496 Fragments arranged by size: 8496 1822 141 BstYI r'GATC_y Cuts at: 1972 3430 3760 4848 4983 5272 6693 6951 7743 Size: 1458 330 1088 135 289 1421 258 792 Cuts at: 7743 7789 8156 8541 8558 10010 10096 10108 10454 Size: 46 367 385 17 1452 86 12 346 Cuts at: 10454 1972 Size: 1977 Fragments arranged by size: 1977 1458 1452 1421 1088 792 385 367 346 330 289 258 135 86 46 17 12 Bsu36I CC'TnA_GG Cuts at: 2976 3203 4085 6893 2976 Size: 227 882 2808 6542 Fragments arranged by size: 6542 2808 882 227 BtgI C'CryG_G Cuts at: 513 1065 2004 2220 2357 7593 513 Size: 552 939 216 137 5236 3379 Fragments arranged by size: 5236 3379 939 552 216 137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9720 Fragments arranged by size: 9720 683 56 BtsI GCAGTG_nn' Cuts at: 2387 3096 7975 8769 8789 2387 Size: 709 4879 794 20 4057 Fragments arranged by size: 4879 4057 794 709 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10459 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10424 DraI TTT'AAA Cuts at: 7763 7763 Size: 10459 DraIII CAC_nnn'GTG Cuts at: 2316 5413 6306 2316 Size: 3097 893 6469 Fragments arranged by size: 6469 3097 893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9466 809 Size: 507 8150 1802 Fragments arranged by size: 8150 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3766 4599 4893 Size: 54 1425 50 275 18 1936 833 294 Cuts at: 4893 5139 5715 6070 6643 7140 7227 7558 7716 Size: 246 576 355 573 497 87 331 158 Cuts at: 7716 7906 8795 10424 8 Size: 190 889 1629 43 Fragments arranged by size: 1936 1629 1425 889 833 576 573 497 355 331 294 275 246 190 158 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7906 1537 Size: 275 6094 4090 Fragments arranged by size: 6094 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2199 2862 3203 4521 4554 5501 5568 5941 Size: 846 663 341 1318 33 947 67 373 Cuts at: 5941 6921 7010 7176 7488 8389 1353 Size: 980 89 166 312 901 3423 Fragments arranged by size: 3423 1318 980 947 901 846 663 373 341 312 166 89 67 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2401 4103 4564 5876 9046 9420 Size: 1064 138 812 1702 461 1312 3170 374 Cuts at: 9420 9566 387 Size: 146 1280 Fragments arranged by size: 3170 1702 1312 1280 1064 812 461 374 146 138 EcoICRI GAG'CTC Cuts at: 2521 2560 2521 Size: 39 10420 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2741 3335 3554 3681 3812 3929 4091 Size: 393 1109 594 219 127 131 117 162 Cuts at: 4091 4232 4373 4383 4892 5297 7028 7062 8522 Size: 141 141 10 509 405 1731 34 1460 Cuts at: 8522 9119 9906 10174 1239 Size: 597 787 268 1524 Fragments arranged by size: 1731 1524 1460 1109 787 597 594 509 405 393 268 219 162 141 141 131 127 117 34 10 EcoNI CCTnn'n_nnAGG Cuts at: 2756 2891 3601 3870 4991 5441 6339 2756 Size: 135 710 269 1121 450 898 6876 Fragments arranged by size: 6876 1121 898 710 450 269 135 EcoO109I rG'GnC_Cy Cuts at: 2066 2761 3054 3199 3860 3907 4143 4406 4539 Size: 695 293 145 661 47 236 263 133 Cuts at: 4539 5774 7000 7096 2066 Size: 1235 1226 96 5429 Fragments arranged by size: 5429 1235 1226 695 661 293 263 236 145 133 96 47 EcoRI G'AATT_C Cuts at: 2044 3334 4109 7768 8146 2044 Size: 1290 775 3659 378 4357 Fragments arranged by size: 4357 3659 1290 775 378 EcoRV GAT'ATC Cuts at: 5643 5643 Size: 10459 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2540 3400 7255 7478 2540 Size: 860 3855 223 5521 Fragments arranged by size: 5521 3855 860 223 FspI TGC'GCA Cuts at: 5507 8965 5507 Size: 3458 7001 HaeII r_GCGC'y Cuts at: 1678 6406 6556 7240 9606 1678 Size: 4728 150 684 2366 2531 Fragments arranged by size: 4728 2531 2366 684 150 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3432 3464 6840 6872 7432 Size: 32 135 32 1860 32 3376 32 560 Cuts at: 7432 7464 9100 9132 9174 9206 1373 Size: 32 1636 32 42 32 2626 Fragments arranged by size: 3376 2626 1860 1636 560 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2860 6239 7803 8049 669 Size: 988 281 922 3379 1564 246 3079 Fragments arranged by size: 3379 3079 1564 988 922 281 246 HindIII A'AGCT_T Cuts at: 748 3184 5070 5832 748 Size: 2436 1886 762 5375 Fragments arranged by size: 5375 2436 1886 762 HpaI GTT'AAC Cuts at: 8049 8049 Size: 10459 KpnI G_GTAC'C Cuts at: 7784 7784 Size: 10459 MfeI C'AATT_G Cuts at: 4015 8058 4015 Size: 4043 6416 MluI A'CGCG_T Cuts at: 10335 10335 Size: 10459 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5352 5670 9573 9757 1305 Size: 4047 318 3903 184 2007 Fragments arranged by size: 4047 3903 2007 318 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3768 4601 5141 5717 6645 Size: 54 1425 343 1936 833 540 576 928 Cuts at: 6645 7142 7229 7560 10 Size: 497 87 331 2909 Fragments arranged by size: 2909 1936 1425 928 833 576 540 497 343 331 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2028 2966 3045 3260 5657 5708 5928 Size: 648 862 938 79 215 2397 51 220 Cuts at: 5928 6171 6424 6705 7566 8417 8776 8935 518 Size: 243 253 281 861 851 359 159 2042 Fragments arranged by size: 2397 2042 938 862 861 851 648 359 281 253 243 220 215 159 79 51 MspA1I CmG'CkG Cuts at: 1606 3481 4546 4637 5110 6357 6466 6483 6766 Size: 1875 1065 91 473 1247 109 17 283 Cuts at: 6766 6919 7033 7875 8552 9700 9945 1606 Size: 153 114 842 677 1148 245 2120 Fragments arranged by size: 2120 1875 1247 1148 1065 842 677 473 283 245 153 114 109 91 17 NcoI C'CATG_G Cuts at: 513 1065 2004 2220 2357 7593 513 Size: 552 939 216 137 5236 3379 Fragments arranged by size: 5236 3379 939 552 216 137 NdeI CA'TA_TG Cuts at: 387 387 Size: 10459 NheI G'CTAG_C Cuts at: 1052 3484 1052 Size: 2432 8027 NotI GC'GGCC_GC Cuts at: 7906 7906 Size: 10459 NsiI A_TGCA'T Cuts at: 3371 5960 6443 7173 3371 Size: 2589 483 730 6657 Fragments arranged by size: 6657 2589 730 483 NspI r_CATG'y Cuts at: 1132 1229 2267 3373 5707 5906 7175 7401 7700 Size: 97 1038 1106 2334 199 1269 226 299 Cuts at: 7700 7817 9362 1132 Size: 117 1545 2229 Fragments arranged by size: 2334 2229 1545 1269 1106 1038 299 226 199 117 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10459 PciI A'CATG_T Cuts at: 7397 9358 7397 Size: 1961 8498 PflMI CCAn_nnn'nTGG Cuts at: 1108 2626 4000 4582 5275 5554 6443 7149 1108 Size: 1518 1374 582 693 279 889 706 4418 Fragments arranged by size: 4418 1518 1374 889 706 693 582 279 PfoI T'CCnGG_A Cuts at: 2757 3558 3570 3579 3865 4908 6018 2757 Size: 801 12 9 286 1043 1110 7198 Fragments arranged by size: 7198 1110 1043 801 286 12 9 PmeI GTTT'AAAC Cuts at: 7763 7763 Size: 10459 PmlI CAC'GTG Cuts at: 7608 7608 Size: 10459 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8528 8560 10041 10073 8528 Size: 32 1481 32 8914 Fragments arranged by size: 8914 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2761 3860 4406 7096 2761 Size: 1099 546 2690 6124 Fragments arranged by size: 6124 2690 1099 546 PshAI GACnn'nnGTC Cuts at: 1613 7101 1613 Size: 5488 4971 PsiI TTA'TAA Cuts at: 8029 8029 Size: 10459 PstI C_TGCA'G Cuts at: 830 2466 2922 4254 4744 5640 5870 6779 7811 Size: 1636 456 1332 490 896 230 909 1032 Cuts at: 7811 830 Size: 3478 Fragments arranged by size: 3478 1636 1332 1032 909 896 490 456 230 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10459 PvuII CAG'CTG Cuts at: 3481 4637 5110 6357 6766 7033 3481 Size: 1156 473 1247 409 267 6907 Fragments arranged by size: 6907 1247 1156 473 409 267 SacI G_AGCT'C Cuts at: 2523 2562 2523 Size: 39 10420 SalI G'TCGA_C Cuts at: 1936 7801 1936 Size: 5865 4594 SapI GCTCTTCn'nnn_ Cuts at: 5501 7010 7488 5501 Size: 1509 478 8472 Fragments arranged by size: 8472 1509 478 SbfI CC_TGCA'GG Cuts at: 7811 7811 Size: 10459 ScaI AGT'ACT Cuts at: 5432 8707 10419 5432 Size: 3275 1712 5472 Fragments arranged by size: 5472 3275 1712 SexAI A'CCwGG_T Cuts at: 2312 7299 2312 Size: 4987 5472 SfcI C'TryA_G Cuts at: 826 1045 2338 2406 2462 2918 3547 4250 4740 Size: 219 1293 68 56 456 629 703 490 Cuts at: 4740 4817 4988 5636 5866 6775 7369 7807 8942 Size: 77 171 648 230 909 594 438 1135 Cuts at: 8942 9623 9814 826 Size: 681 191 1471 Fragments arranged by size: 1471 1293 1135 909 703 681 648 629 594 490 456 438 230 219 191 171 77 68 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10459 SgrAI Cr'CCGG_yG Cuts at: 1700 5997 1700 Size: 4297 6162 SmaI CCC'GGG Cuts at: 1872 7786 1872 Size: 5914 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 5039 5156 5297 6221 6995 Size: 197 926 612 2484 117 141 924 774 Cuts at: 6995 7240 7388 8563 9464 9726 820 Size: 245 148 1175 901 262 1553 Fragments arranged by size: 2484 1553 1175 926 924 901 774 612 262 245 197 148 141 117 SnaBI TAC'GTA Cuts at: 493 8250 493 Size: 7757 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10459 SphI G_CATG'C Cuts at: 1132 2267 7700 7817 1132 Size: 1135 5433 117 3774 Fragments arranged by size: 5433 3774 1135 117 SspI AAT'ATT Cuts at: 5 52 6723 8383 5 Size: 47 6671 1660 2081 Fragments arranged by size: 6671 2081 1660 47 StuI AGG'CCT Cuts at: 1517 3545 6267 1517 Size: 2028 2722 5709 Fragments arranged by size: 5709 2722 2028 StyI C'CwwG_G Cuts at: 513 1065 2004 2165 2220 2357 3375 3641 5566 Size: 552 939 161 55 137 1018 266 1925 Cuts at: 5566 5718 7593 7898 513 Size: 152 1875 305 3074 Fragments arranged by size: 3074 1925 1875 1018 939 552 305 266 161 152 137 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4034 6183 6731 7424 8486 8503 8656 Size: 1255 2454 2149 548 693 1062 17 153 Cuts at: 8656 8841 9286 325 Size: 185 445 1498 Fragments arranged by size: 2454 2149 1498 1255 1062 693 548 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2623 4257 5430 Size: 274 80 33 51 1443 646 1634 1173 Cuts at: 5430 5688 6623 8705 10417 96 Size: 258 935 2082 1712 138 Fragments arranged by size: 2082 1712 1634 1443 1173 935 646 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4045 4077 10419 10451 4045 Size: 32 6342 32 4053 Fragments arranged by size: 6342 4053 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10459 XbaI T'CTAG_A Cuts at: 7795 7795 Size: 10459 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4622 6653 6725 4622 Size: 2031 72 8356 Fragments arranged by size: 8356 2031 72 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10459 XmaI C'CCGG_G Cuts at: 1870 7784 1870 Size: 5914 4545 XmnI GAAnn'nnTTC Cuts at: 2457 3591 4076 8588 2457 Size: 1134 485 4512 4328 Fragments arranged by size: 4512 4328 1134 485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8267 276 Size: 53 83 186 936 6733 2468 Fragments arranged by size: 6733 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI BbeI BsiWI BsmBI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI