Restriction map for pFN21AA1404
 (Circular) (Six-base) MAPSORT of: pFN21AA1404.seq  Check: 5492  from: 1  to: 10459



pFN21AA1404  10459 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:31  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    8269     278 
   Size:         53      83     186     936    6733    2468

  Fragments arranged by size: 

                6733    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3285    7802    1937 
   Size:       1348    4517    4594

  Fragments arranged by size: 

                4594    4517    1348
 
Acc65I G'GTAC_C

Cuts at:   7780    7780 
   Size:      10459
 
AclI AA'CG_TT

Cuts at:   1563    8586    8959    1563 
   Size:       7023     373    3063

  Fragments arranged by size: 

                7023    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3335    3681    3812    4091    7062    8522    9906   10174 
   Size:       1703     346     131     279    2971    1460    1384     268

Cuts at:  10174    1632 
   Size:       1917

  Fragments arranged by size: 

                2971    1917    1703    1460    1384     346     279     268
                 131
 
AfeI AGC'GCT

Cuts at:   7238    7238 
   Size:      10459
 
AflII C'TTAA_G

Cuts at:    820    1017    5297    6995     820 
   Size:        197    4280    1698    4284

  Fragments arranged by size: 

                4284    4280    1698     197
 
AflIII A'CryG_T

Cuts at:   1203    5127    7397    7607    9358   10335    1203 
   Size:       3924    2270     210    1751     977    1327

  Fragments arranged by size: 

                3924    2270    1751    1327     977     210
 
AgeI A'CCGG_T

Cuts at:   5997    6241    9290    5997 
   Size:        244    3049    7166

  Fragments arranged by size: 

                7166    3049     244
 
AhdI GACnn_n'nnGTC

Cuts at:   9188    9188 
   Size:      10459
 
AleI CACnn'nnGTG

Cuts at:   1166    2966    6171    6424    1166 
   Size:       1800    3205     253    5201

  Fragments arranged by size: 

                5201    3205    1800     253
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    5531    6422    1184 
   Size:       4347     891    5221

  Fragments arranged by size: 

                5221    4347     891
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5352    5384    6069    6101    5352 
   Size:         32     685      32    9710

  Fragments arranged by size: 

                9710     685      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   3202    3704    4000    4249    4409    4637    4967    5110    5275 
   Size:        502     296     249     160     228     330     143     165

Cuts at:   5275    5713    8202    9774    3202 
   Size:        438    2489    1572    3887

  Fragments arranged by size: 

                3887    2489    1572     502     438     330     296     249
                 228     165     160     143
 
ApaLI G'TGCA_C

Cuts at:   3038    4216    6571    8516    9672    3038 
   Size:       1178    2355    1945    1156    3825

  Fragments arranged by size: 

                3825    2355    1945    1178    1156
 
ApoI r'AATT_y

Cuts at:   1493    2044    2648    3334    3710    4109    7768    7994    8146 
   Size:        551     604     686     376     399    3659     226     152

Cuts at:   8146   10255    1493 
   Size:       2109    1697

  Fragments arranged by size: 

                3659    2109    1697     686     604     551     399     376
                 226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3750    9013     160 
   Size:        872    2718    5263    1606

  Fragments arranged by size: 

                5263    2718    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10459
 
AvaI C'yCGr_G

Cuts at:   1870    1943    5670    7784    1870 
   Size:         73    3727    2114    4545

  Fragments arranged by size: 

                4545    3727    2114      73
 
AvrII C'CTAG_G

Cuts at:   2165    2165 
   Size:      10459
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   4138    4171    4808    4841    4853    4886    5808    5841    4138 
   Size:         33     637      33      12      33     922      33    8756

  Fragments arranged by size: 

                8756     922     637      33      33      33      33      12
            
 
BamHI G'GATC_C

Cuts at:   7789    8156    7789 
   Size:        367   10092
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    4773    5722    5994    6046    6874 
   Size:        325     217     627    2986     949     272      52     828

Cuts at:   6874    7780    9235     618 
   Size:        906    1455    1842

  Fragments arranged by size: 

                2986    1842    1455     949     906     828     627     325
                 272     217      52
 
BanII G_rGCy'C

Cuts at:   1391    2523    2562    6594    6962    7693    7778    1391 
   Size:       1132      39    4032     368     731      85    4072

  Fragments arranged by size: 

                4072    4032    1132     731     368      85      39
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2023    2450    2767    2991    3198    4031    4920 
   Size:        971     124     427     317     224     207     833     889

Cuts at:   4920    5316    6777    7050     928 
   Size:        396    1461     273    4337

  Fragments arranged by size: 

                4337    1461     971     889     833     427     396     317
                 273     224     207     124
 
BbvCI CC'TCA_GC

Cuts at:   4094    4853    4094 
   Size:        759    9700
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3080    3114    6591    6625    8650    8684    1903 
   Size:         34    1143      34    3477      34    2025      34    3678

  Fragments arranged by size: 

                3678    3477    2025    1143      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   2733    2947    3106    5836    8351    9561    2733 
   Size:        214     159    2730    2515    1210    3631

  Fragments arranged by size: 

                3631    2730    2515    1210     214     159
 
BclI T'GATC_A

Cuts at:   1548    2688    1548 
   Size:       1140    9319
 
BfrBI ATG'CAT

Cuts at:   3369    5958    6441    7171    3369 
   Size:       2589     483     730    6657

  Fragments arranged by size: 

                6657    2589     730     483
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4546    7557    9070 
   Size:        107     122      71     631     605    2874    3011    1513

Cuts at:   9070     136 
   Size:       1525

  Fragments arranged by size: 

                3011    2874    1525    1513     631     605     122     107
                  71
 
BglII A'GATC_T

Cuts at:   3430   10454    3430 
   Size:       7024    3435
 
BlpI GC'TnA_GC

Cuts at:   6961    7876    6961 
   Size:        915    9544
 
Bme1580I G_kGCm'C

Cuts at:   1790    2942    3042    4220    5234    5727    5997    6051    6156 
   Size:       1152     100    1178    1014     493     270      54     105

Cuts at:   6156    6234    6455    6575    6879    7607    8520    9676    1790 
   Size:         78     221     120     304     728     913    1156    2573

  Fragments arranged by size: 

                2573    1178    1156    1152    1014     913     728     493
                 304     270     221     120     105     100      78      54
            
 
BmgBI CAC'GTC

Cuts at:   1318    3556    1318 
   Size:       2238    8221
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2444    2636    3714 
   Size:        416      53      83     399      24    1027     192    1078

Cuts at:   3714    4285    4635    5112    8190    9148     442 
   Size:        571     350     477    3078     958    1753

  Fragments arranged by size: 

                3078    1753    1078    1027     958     571     477     416
                 399     350     192      83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    3488    1056 
   Size:       2432    8027
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2037    3640    6148    2037 
   Size:       1603    2508    6348

  Fragments arranged by size: 

                6348    2508    1603
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2741    3554    3929    4232    4373    4383    4892    5297 
   Size:       1502     813     375     303     141      10     509     405

Cuts at:   5297    7028    9119    1239 
   Size:       1731    2091    2579

  Fragments arranged by size: 

                2579    2091    1731    1502     813     509     405     375
                 303     141      10
 
Bpu10I CC'TnA_GC

Cuts at:   4094    4853    4094 
   Size:        759    9700
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2576    5060    5177    6206    7261    7409    8584    9449    9747 
   Size:       2484     117    1029    1055     148    1175     865     298

Cuts at:   9747    2576 
   Size:       3288

  Fragments arranged by size: 

                3288    2484    1175    1055    1029     865     298     148
                 117
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2277    6665    9122     882 
   Size:        617     778    4388    2457    2219

  Fragments arranged by size: 

                4388    2457    2219     778     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    7608    8250     493 
   Size:        711    6404     642    2702

  Fragments arranged by size: 

                6404    2702     711     642
 
BsaBI GATnn'nnATC

Cuts at:   4475    5468    6935   10453    4475 
   Size:        993    1467    3518    4481

  Fragments arranged by size: 

                4481    3518    1467     993
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    5538    8266    8648    9283 
   Size:         53      83     186     936    4005    2728     382     635

Cuts at:   9283     275 
   Size:       1451

  Fragments arranged by size: 

                4005    2728    1451     936     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   5997    6241    8892    9290    9564    9711   10122    5997 
   Size:        244    2651     398     274     147     411    6334

  Fragments arranged by size: 

                6334    2651     411     398     274     244     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2732    2762    3629    3659    4045 
   Size:         30     165      30    1329      30     867      30     386

Cuts at:   4045    4075    1178 
   Size:         30    7562

  Fragments arranged by size: 

                7562    1329     867     386     165      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2024    2151    2154    2744    4454    4469    4472    4535 
   Size:        838     127       3     590    1710      15       3      63

Cuts at:   4535    5582    7013    7754    1186 
   Size:       1047    1431     741    3891

  Fragments arranged by size: 

                3891    1710    1431    1047     838     741     590     127
                  63      15       3       3
 
BseYI C'CCAG_C

Cuts at:   4269    4542    4671    7692    9662   10158    4269 
   Size:        273     129    3021    1970     496    4570

  Fragments arranged by size: 

                4570    3021    1970     496     273     129
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2813    2990    3314    4322    4904    5893    6209 
   Size:        573    1077     177     324    1008     582     989     316

Cuts at:   6209    6782    7451    7523    1163 
   Size:        573     669      72    4099

  Fragments arranged by size: 

                4099    1077    1008     989     669     582     573     573
                 324     316     177      72
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    7909    8670    9698    1540 
   Size:        275     186    5908     761    1028    2301

  Fragments arranged by size: 

                5908    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2523    2562    2733    3042    4220    6575    8520    8605    9676 
   Size:         39     171     309    1178    2355    1945      85    1071

Cuts at:   9676    2523 
   Size:       3306

  Fragments arranged by size: 

                3306    2355    1945    1178    1071     309     171      85
                  39
 
BsmI GAATG_Cn'

Cuts at:   4339    7970    8063    4339 
   Size:       3631      93    6735

  Fragments arranged by size: 

                6735    3631      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2523    2562    2733    2942    3042    4220    5234 
   Size:        399     733      39     171     209     100    1178    1014

Cuts at:   5234    5727    5997    6051    6156    6234    6455    6575    6594 
   Size:        493     270      54     105      78     221     120      19

Cuts at:   6594    6879    6962    7607    7693    7778    8520    8605    9676 
   Size:        285      83     645      86      85     742      85    1071

Cuts at:   9676    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1178    1071    1014     742     733     645     493
                 399     285     270     221     209     171     120     105
                 100      86      85      85      83      78      54      39
                  19
 
BspEI T'CCGG_A

Cuts at:  10122   10122 
   Size:      10459
 
BspHI T'CATG_A

Cuts at:   4158    4192    4287    8348   10078    4158 
   Size:         34      95    4061    1730    4539

  Fragments arranged by size: 

                4539    4061    1730      95      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    4257    4926    5460    7814     844 
   Size:       3413     669     534    2354    3489

  Fragments arranged by size: 

                3489    3413    2354     669     534
 
BsrBI CCG'CTC

Cuts at:   1394    1823    8346    1394 
   Size:        429    6523    3507

  Fragments arranged by size: 

                6523    3507     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    5052    7561    8954    9128      65 
   Size:       1183    3804    2509    1393     174    1396

  Fragments arranged by size: 

                3804    2509    1396    1393    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5997    6241    9103    9290    1700 
   Size:        110    4187     244    2862     187    2869

  Fragments arranged by size: 

                4187    2869    2862     244     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:      10459
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:      10459
 
BssSI C'ACGA_G

Cuts at:   7530    8519    9531    7530 
   Size:        989    1012    8458

  Fragments arranged by size: 

                8458    1012     989
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    5073     794 
   Size:       4279    6180
 
BstBI TT'CG_AA

Cuts at:   6363    9263    6363 
   Size:       2900    7559
 
BstEII G'GTnAC_C

Cuts at:   1184    6208    6712    1184 
   Size:       5024     504    4931

  Fragments arranged by size: 

                5024    4931     504
 
BstXI CCAn_nnnn'nTGG

Cuts at:   4210    4351    6173    4210 
   Size:        141    1822    8496

  Fragments arranged by size: 

                8496    1822     141
 
BstYI r'GATC_y

Cuts at:   1972    3430    3760    4848    4983    5272    6693    6951    7743 
   Size:       1458     330    1088     135     289    1421     258     792

Cuts at:   7743    7789    8156    8541    8558   10010   10096   10108   10454 
   Size:         46     367     385      17    1452      86      12     346

Cuts at:  10454    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1458    1452    1421    1088     792     385     367
                 346     330     289     258     135      86      46      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2976    3203    4085    6893    2976 
   Size:        227     882    2808    6542

  Fragments arranged by size: 

                6542    2808     882     227
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2220    2357    7593     513 
   Size:        552     939     216     137    5236    3379

  Fragments arranged by size: 

                5236    3379     939     552     216     137
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    9720

  Fragments arranged by size: 

                9720     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2387    3096    7975    8769    8789    2387 
   Size:        709    4879     794      20    4057

  Fragments arranged by size: 

                4879    4057     794     709      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:      10459
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35   10424
 
DraI TTT'AAA

Cuts at:   7763    7763 
   Size:      10459
 
DraIII CAC_nnn'GTG

Cuts at:   2316    5413    6306    2316 
   Size:       3097     893    6469

  Fragments arranged by size: 

                6469    3097     893
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    9466     809 
   Size:        507    8150    1802

  Fragments arranged by size: 

                8150    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3766    4599    4893 
   Size:         54    1425      50     275      18    1936     833     294

Cuts at:   4893    5139    5715    6070    6643    7140    7227    7558    7716 
   Size:        246     576     355     573     497      87     331     158

Cuts at:   7716    7906    8795   10424       8 
   Size:        190     889    1629      43

  Fragments arranged by size: 

                1936    1629    1425     889     833     576     573     497
                 355     331     294     275     246     190     158      87
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    7906    1537 
   Size:        275    6094    4090

  Fragments arranged by size: 

                6094    4090     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2199    2862    3203    4521    4554    5501    5568    5941 
   Size:        846     663     341    1318      33     947      67     373

Cuts at:   5941    6921    7010    7176    7488    8389    1353 
   Size:        980      89     166     312     901    3423

  Fragments arranged by size: 

                3423    1318     980     947     901     846     663     373
                 341     312     166      89      67      33
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2401    4103    4564    5876    9046    9420 
   Size:       1064     138     812    1702     461    1312    3170     374

Cuts at:   9420    9566     387 
   Size:        146    1280

  Fragments arranged by size: 

                3170    1702    1312    1280    1064     812     461     374
                 146     138
 
EcoICRI GAG'CTC

Cuts at:   2521    2560    2521 
   Size:         39   10420
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2741    3335    3554    3681    3812    3929    4091 
   Size:        393    1109     594     219     127     131     117     162

Cuts at:   4091    4232    4373    4383    4892    5297    7028    7062    8522 
   Size:        141     141      10     509     405    1731      34    1460

Cuts at:   8522    9119    9906   10174    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1731    1524    1460    1109     787     597     594     509
                 405     393     268     219     162     141     141     131
                 127     117      34      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2756    2891    3601    3870    4991    5441    6339    2756 
   Size:        135     710     269    1121     450     898    6876

  Fragments arranged by size: 

                6876    1121     898     710     450     269     135
 
EcoO109I rG'GnC_Cy

Cuts at:   2066    2761    3054    3199    3860    3907    4143    4406    4539 
   Size:        695     293     145     661      47     236     263     133

Cuts at:   4539    5774    7000    7096    2066 
   Size:       1235    1226      96    5429

  Fragments arranged by size: 

                5429    1235    1226     695     661     293     263     236
                 145     133      96      47
 
EcoRI G'AATT_C

Cuts at:   2044    3334    4109    7768    8146    2044 
   Size:       1290     775    3659     378    4357

  Fragments arranged by size: 

                4357    3659    1290     775     378
 
EcoRV GAT'ATC

Cuts at:   5643    5643 
   Size:      10459
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2540    3400    7255    7478    2540 
   Size:        860    3855     223    5521

  Fragments arranged by size: 

                5521    3855     860     223
 
FspI TGC'GCA

Cuts at:   5507    8965    5507 
   Size:       3458    7001
 
HaeII r_GCGC'y

Cuts at:   1678    6406    6556    7240    9606    1678 
   Size:       4728     150     684    2366    2531

  Fragments arranged by size: 

                4728    2531    2366     684     150
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3432    3464    6840    6872    7432 
   Size:         32     135      32    1860      32    3376      32     560

Cuts at:   7432    7464    9100    9132    9174    9206    1373 
   Size:         32    1636      32      42      32    2626

  Fragments arranged by size: 

                3376    2626    1860    1636     560     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2860    6239    7803    8049     669 
   Size:        988     281     922    3379    1564     246    3079

  Fragments arranged by size: 

                3379    3079    1564     988     922     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3184    5070    5832     748 
   Size:       2436    1886     762    5375

  Fragments arranged by size: 

                5375    2436    1886     762
 
HpaI GTT'AAC

Cuts at:   8049    8049 
   Size:      10459
 
KpnI G_GTAC'C

Cuts at:   7784    7784 
   Size:      10459
 
MfeI C'AATT_G

Cuts at:   4015    8058    4015 
   Size:       4043    6416
 
MluI A'CGCG_T

Cuts at:  10335   10335 
   Size:      10459
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5352    5670    9573    9757    1305 
   Size:       4047     318    3903     184    2007

  Fragments arranged by size: 

                4047    3903    2007     318     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    3768    4601    5141    5717    6645 
   Size:         54    1425     343    1936     833     540     576     928

Cuts at:   6645    7142    7229    7560      10 
   Size:        497      87     331    2909

  Fragments arranged by size: 

                2909    1936    1425     928     833     576     540     497
                 343     331      87      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2028    2966    3045    3260    5657    5708    5928 
   Size:        648     862     938      79     215    2397      51     220

Cuts at:   5928    6171    6424    6705    7566    8417    8776    8935     518 
   Size:        243     253     281     861     851     359     159    2042

  Fragments arranged by size: 

                2397    2042     938     862     861     851     648     359
                 281     253     243     220     215     159      79      51
            
 
MspA1I CmG'CkG

Cuts at:   1606    3481    4546    4637    5110    6357    6466    6483    6766 
   Size:       1875    1065      91     473    1247     109      17     283

Cuts at:   6766    6919    7033    7875    8552    9700    9945    1606 
   Size:        153     114     842     677    1148     245    2120

  Fragments arranged by size: 

                2120    1875    1247    1148    1065     842     677     473
                 283     245     153     114     109      91      17
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2220    2357    7593     513 
   Size:        552     939     216     137    5236    3379

  Fragments arranged by size: 

                5236    3379     939     552     216     137
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:      10459
 
NheI G'CTAG_C

Cuts at:   1052    3484    1052 
   Size:       2432    8027
 
NotI GC'GGCC_GC

Cuts at:   7906    7906 
   Size:      10459
 
NsiI A_TGCA'T

Cuts at:   3371    5960    6443    7173    3371 
   Size:       2589     483     730    6657

  Fragments arranged by size: 

                6657    2589     730     483
 
NspI r_CATG'y

Cuts at:   1132    1229    2267    3373    5707    5906    7175    7401    7700 
   Size:         97    1038    1106    2334     199    1269     226     299

Cuts at:   7700    7817    9362    1132 
   Size:        117    1545    2229

  Fragments arranged by size: 

                2334    2229    1545    1269    1106    1038     299     226
                 199     117      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:      10459
 
PciI A'CATG_T

Cuts at:   7397    9358    7397 
   Size:       1961    8498
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2626    4000    4582    5275    5554    6443    7149    1108 
   Size:       1518    1374     582     693     279     889     706    4418

  Fragments arranged by size: 

                4418    1518    1374     889     706     693     582     279
            
 
PfoI T'CCnGG_A

Cuts at:   2757    3558    3570    3579    3865    4908    6018    2757 
   Size:        801      12       9     286    1043    1110    7198

  Fragments arranged by size: 

                7198    1110    1043     801     286      12       9
 
PmeI GTTT'AAAC

Cuts at:   7763    7763 
   Size:      10459
 
PmlI CAC'GTG

Cuts at:   7608    7608 
   Size:      10459
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   8528    8560   10041   10073    8528 
   Size:         32    1481      32    8914

  Fragments arranged by size: 

                8914    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2761    3860    4406    7096    2761 
   Size:       1099     546    2690    6124

  Fragments arranged by size: 

                6124    2690    1099     546
 
PshAI GACnn'nnGTC

Cuts at:   1613    7101    1613 
   Size:       5488    4971
 
PsiI TTA'TAA

Cuts at:   8029    8029 
   Size:      10459
 
PstI C_TGCA'G

Cuts at:    830    2466    2922    4254    4744    5640    5870    6779    7811 
   Size:       1636     456    1332     490     896     230     909    1032

Cuts at:   7811     830 
   Size:       3478

  Fragments arranged by size: 

                3478    1636    1332    1032     909     896     490     456
                 230
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:      10459
 
PvuII CAG'CTG

Cuts at:   3481    4637    5110    6357    6766    7033    3481 
   Size:       1156     473    1247     409     267    6907

  Fragments arranged by size: 

                6907    1247    1156     473     409     267
 
SacI G_AGCT'C

Cuts at:   2523    2562    2523 
   Size:         39   10420
 
SalI G'TCGA_C

Cuts at:   1936    7801    1936 
   Size:       5865    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   5501    7010    7488    5501 
   Size:       1509     478    8472

  Fragments arranged by size: 

                8472    1509     478
 
SbfI CC_TGCA'GG

Cuts at:   7811    7811 
   Size:      10459
 
ScaI AGT'ACT

Cuts at:   5432    8707   10419    5432 
   Size:       3275    1712    5472

  Fragments arranged by size: 

                5472    3275    1712
 
SexAI A'CCwGG_T

Cuts at:   2312    7299    2312 
   Size:       4987    5472
 
SfcI C'TryA_G

Cuts at:    826    1045    2338    2406    2462    2918    3547    4250    4740 
   Size:        219    1293      68      56     456     629     703     490

Cuts at:   4740    4817    4988    5636    5866    6775    7369    7807    8942 
   Size:         77     171     648     230     909     594     438    1135

Cuts at:   8942    9623    9814     826 
   Size:        681     191    1471

  Fragments arranged by size: 

                1471    1293    1135     909     703     681     648     629
                 594     490     456     438     230     219     191     171
                  77      68      56
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10459
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    5997    1700 
   Size:       4297    6162
 
SmaI CCC'GGG

Cuts at:   1872    7786    1872 
   Size:       5914    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2555    5039    5156    5297    6221    6995 
   Size:        197     926     612    2484     117     141     924     774

Cuts at:   6995    7240    7388    8563    9464    9726     820 
   Size:        245     148    1175     901     262    1553

  Fragments arranged by size: 

                2484    1553    1175     926     924     901     774     612
                 262     245     197     148     141     117
 
SnaBI TAC'GTA

Cuts at:    493    8250     493 
   Size:       7757    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:      10459
 
SphI G_CATG'C

Cuts at:   1132    2267    7700    7817    1132 
   Size:       1135    5433     117    3774

  Fragments arranged by size: 

                5433    3774    1135     117
 
SspI AAT'ATT

Cuts at:      5      52    6723    8383       5 
   Size:         47    6671    1660    2081

  Fragments arranged by size: 

                6671    2081    1660      47
 
StuI AGG'CCT

Cuts at:   1517    3545    6267    1517 
   Size:       2028    2722    5709

  Fragments arranged by size: 

                5709    2722    2028
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2165    2220    2357    3375    3641    5566 
   Size:        552     939     161      55     137    1018     266    1925

Cuts at:   5566    5718    7593    7898     513 
   Size:        152    1875     305    3074

  Fragments arranged by size: 

                3074    1925    1875    1018     939     552     305     266
                 161     152     137      55
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4034    6183    6731    7424    8486    8503    8656 
   Size:       1255    2454    2149     548     693    1062      17     153

Cuts at:   8656    8841    9286     325 
   Size:        185     445    1498

  Fragments arranged by size: 

                2454    2149    1498    1255    1062     693     548     445
                 185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2623    4257    5430 
   Size:        274      80      33      51    1443     646    1634    1173

Cuts at:   5430    5688    6623    8705   10417      96 
   Size:        258     935    2082    1712     138

  Fragments arranged by size: 

                2082    1712    1634    1443    1173     935     646     274
                 258     138      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4045    4077   10419   10451    4045 
   Size:         32    6342      32    4053

  Fragments arranged by size: 

                6342    4053      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:      10459
 
XbaI T'CTAG_A

Cuts at:   7795    7795 
   Size:      10459
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   4622    6653    6725    4622 
   Size:       2031      72    8356

  Fragments arranged by size: 

                8356    2031      72
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:      10459
 
XmaI C'CCGG_G

Cuts at:   1870    7784    1870 
   Size:       5914    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2457    3591    4076    8588    2457 
   Size:       1134     485    4512    4328

  Fragments arranged by size: 

                4512    4328    1134     485
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    8267     276 
   Size:         53      83     186     936    6733    2468

  Fragments arranged by size: 

                6733    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     ApaI     AscI     BbeI    BsiWI    BsmBI  BstZ17I     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     PacI   PspOMI
    PspXI     PsrI    RsrII    SacII    SanDI     SfiI     SfoI     SrfI
     SwaI
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