(Circular) (Six-base) MAPSORT of: pFN21AA1378.seq Check: 5081 from: 1 to: 6565 pFN21AA1378 6565 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4375 278 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3908 1937 Size: 1971 4594 Acc65I G'GTAC_C Cuts at: 3886 3886 Size: 6565 AclI AA'CG_TT Cuts at: 1563 4692 5065 1563 Size: 3129 373 3063 Fragments arranged by size: 3129 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1995 2328 2616 4628 6012 6280 1632 Size: 363 333 288 2012 1384 268 1917 Fragments arranged by size: 2012 1917 1384 363 333 288 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6368 AflIII A'CryG_T Cuts at: 1203 3076 5464 6441 1203 Size: 1873 2388 977 1327 Fragments arranged by size: 2388 1873 1327 977 AgeI A'CCGG_T Cuts at: 5396 5396 Size: 6565 AhdI GACnn_n'nnGTC Cuts at: 5294 5294 Size: 6565 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6565 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6565 AlwNI CAG_nnn'CTG Cuts at: 2909 4308 5880 2909 Size: 1399 1572 3594 Fragments arranged by size: 3594 1572 1399 ApaLI G'TGCA_C Cuts at: 4622 5778 4622 Size: 1156 5409 ApoI r'AATT_y Cuts at: 1493 3819 3874 4100 4252 6361 1493 Size: 2326 55 226 152 2109 1697 Fragments arranged by size: 2326 2109 1697 226 152 55 AseI AT'TA_AT Cuts at: 160 1032 5119 160 Size: 872 4087 1606 Fragments arranged by size: 4087 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 AvaI C'yCGr_G Cuts at: 1870 1943 3890 1870 Size: 73 1947 4545 Fragments arranged by size: 4545 1947 73 BamHI G'GATC_C Cuts at: 3839 3895 4262 3839 Size: 56 367 6142 Fragments arranged by size: 6142 367 56 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3334 3886 5341 618 Size: 325 217 627 1547 552 1455 1842 Fragments arranged by size: 1842 1547 1455 627 552 325 217 BanII G_rGCy'C Cuts at: 1391 3884 1391 Size: 2493 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2367 2619 928 Size: 971 468 252 4874 Fragments arranged by size: 4874 971 468 252 BbvCI CC'TCA_GC Cuts at: 2730 2730 Size: 6565 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4756 4790 1903 Size: 34 2819 34 3678 Fragments arranged by size: 3678 2819 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4457 5667 4457 Size: 1210 5355 BclI T'GATC_A Cuts at: 1548 3316 1548 Size: 1768 4797 BfrBI ATG'CAT Cuts at: 3701 3701 Size: 6565 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5176 136 Size: 107 122 71 631 605 3504 1525 Fragments arranged by size: 3504 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6560 6560 Size: 6565 BlpI GC'TnA_GC Cuts at: 3982 3982 Size: 6565 Bme1580I G_kGCm'C Cuts at: 1790 4626 5782 1790 Size: 2836 1156 2573 Fragments arranged by size: 2836 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6565 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4296 5254 442 Size: 416 53 83 399 24 2879 958 1753 Fragments arranged by size: 2879 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6565 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2644 3801 5225 1239 Size: 1405 1157 1424 2579 Fragments arranged by size: 2579 1424 1405 1157 Bpu10I CC'TnA_GC Cuts at: 2730 2730 Size: 6565 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2201 2911 4690 5555 5853 2201 Size: 710 1779 865 298 2913 Fragments arranged by size: 2913 1779 865 710 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5228 882 Size: 617 3729 2219 Fragments arranged by size: 3729 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3535 4356 493 Size: 711 2331 821 2702 Fragments arranged by size: 2702 2331 821 711 BsaBI GATnn'nnATC Cuts at: 3450 6559 3450 Size: 3109 3456 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4372 4754 5389 275 Size: 53 83 186 936 2839 382 635 1451 Fragments arranged by size: 2839 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4998 5396 5670 5817 6228 4998 Size: 398 274 147 411 5335 Fragments arranged by size: 5335 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3774 3780 3804 3810 1178 Size: 30 165 30 2371 6 24 6 3933 Fragments arranged by size: 3933 2371 165 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3372 3552 1186 Size: 2186 180 4199 Fragments arranged by size: 4199 2186 180 BseYI C'CCAG_C Cuts at: 5768 6264 5768 Size: 496 6069 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5992 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4015 4776 5804 1540 Size: 275 186 2014 761 1028 2301 Fragments arranged by size: 2301 2014 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4626 4711 5782 4626 Size: 85 1071 5409 Fragments arranged by size: 5409 1071 85 BsmI GAATG_Cn' Cuts at: 3020 4076 4169 3020 Size: 1056 93 5416 Fragments arranged by size: 5416 1056 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3884 4626 4711 5782 1391 Size: 399 2094 742 85 1071 2174 Fragments arranged by size: 2174 2094 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6228 6228 Size: 6565 BspHI T'CATG_A Cuts at: 4454 6184 4454 Size: 1730 4835 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2437 2680 3920 844 Size: 1593 243 1240 3489 Fragments arranged by size: 3489 1593 1240 243 BsrBI CCG'CTC Cuts at: 1394 1823 3580 4452 1394 Size: 429 1757 872 3507 Fragments arranged by size: 3507 1757 872 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3412 3508 3703 5060 5234 65 Size: 1183 2164 96 195 1357 174 1396 Fragments arranged by size: 2164 1396 1357 1183 195 174 96 BsrFI r'CCGG_y Cuts at: 1700 1810 2788 5209 5396 1700 Size: 110 978 2421 187 2869 Fragments arranged by size: 2869 2421 978 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6565 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6565 BssSI C'ACGA_G Cuts at: 4625 5637 4625 Size: 1012 5553 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6565 BstBI TT'CG_AA Cuts at: 5369 5369 Size: 6565 BstEII G'GTnAC_C Cuts at: 1184 2993 1184 Size: 1809 4756 BstXI CCAn_nnnn'nTGG Cuts at: 2512 2749 3850 2512 Size: 237 1101 5227 Fragments arranged by size: 5227 1101 237 BstYI r'GATC_y Cuts at: 1972 2093 2987 3758 3839 3895 4262 4647 4664 Size: 121 894 771 81 56 367 385 17 Cuts at: 4664 6116 6202 6214 6560 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 894 771 385 367 346 121 86 81 56 17 12 Bsu36I CC'TnA_GG Cuts at: 3235 3235 Size: 6565 BtgI C'CryG_G Cuts at: 513 1065 2004 3352 513 Size: 552 939 1348 3726 Fragments arranged by size: 3726 1348 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5826 Fragments arranged by size: 5826 683 56 BtsI GCAGTG_nn' Cuts at: 3769 4081 4875 4895 3769 Size: 312 794 20 5439 Fragments arranged by size: 5439 794 312 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6565 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6530 DraI TTT'AAA Cuts at: 3869 3869 Size: 6565 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5572 809 Size: 507 4256 1802 Fragments arranged by size: 4256 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4012 4901 6530 Size: 54 1425 50 275 18 2182 889 1629 Cuts at: 6530 8 Size: 43 Fragments arranged by size: 2182 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4012 1537 Size: 275 2200 4090 Fragments arranged by size: 4090 2200 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2178 3487 4495 1353 Size: 825 1309 1008 3423 Fragments arranged by size: 3423 1309 1008 825 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2582 5152 5526 5672 387 Size: 1064 138 993 2570 374 146 1280 Fragments arranged by size: 2570 1280 1064 993 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1995 2328 2616 2644 3801 4628 5225 Size: 393 363 333 288 28 1157 827 597 Cuts at: 5225 6012 6280 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1157 827 787 597 393 363 333 288 268 28 EcoRI G'AATT_C Cuts at: 3874 4252 3874 Size: 378 6187 FspI TGC'GCA Cuts at: 5071 5071 Size: 6565 HaeII r_GCGC'y Cuts at: 1678 5712 1678 Size: 4034 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3148 3180 3296 3328 3430 Size: 32 135 32 1576 32 116 32 102 Cuts at: 3430 3462 3633 3665 5206 5238 5280 5312 1373 Size: 32 171 32 1541 32 42 32 2626 Fragments arranged by size: 2626 1576 1541 171 135 116 102 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2407 3856 3909 4155 669 Size: 988 281 469 1449 53 246 3079 Fragments arranged by size: 3079 1449 988 469 281 246 53 HindIII A'AGCT_T Cuts at: 748 2148 2648 3515 748 Size: 1400 500 867 3798 Fragments arranged by size: 3798 1400 867 500 HpaI GTT'AAC Cuts at: 4155 4155 Size: 6565 KpnI G_GTAC'C Cuts at: 3890 3890 Size: 6565 MfeI C'AATT_G Cuts at: 2506 3255 4164 2506 Size: 749 909 4907 Fragments arranged by size: 4907 909 749 MluI A'CGCG_T Cuts at: 6441 6441 Size: 6565 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2730 3736 5679 5863 1305 Size: 1425 1006 1943 184 2007 Fragments arranged by size: 2007 1943 1425 1006 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4743 Fragments arranged by size: 4743 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3081 3390 4523 4882 5041 518 Size: 648 1915 309 1133 359 159 2042 Fragments arranged by size: 2042 1915 1133 648 359 309 159 MspA1I CmG'CkG Cuts at: 1606 3981 4658 5806 6051 1606 Size: 2375 677 1148 245 2120 Fragments arranged by size: 2375 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5074 Fragments arranged by size: 5074 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6565 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6565 NotI GC'GGCC_GC Cuts at: 4012 4012 Size: 6565 NsiI A_TGCA'T Cuts at: 3703 3703 Size: 6565 NspI r_CATG'y Cuts at: 1132 1229 3080 3923 5468 1132 Size: 97 1851 843 1545 2229 Fragments arranged by size: 2229 1851 1545 843 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6565 PciI A'CATG_T Cuts at: 3076 5464 3076 Size: 2388 4177 PflMI CCAn_nnn'nTGG Cuts at: 1108 2712 1108 Size: 1604 4961 PmeI GTTT'AAAC Cuts at: 3869 3869 Size: 6565 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2081 2113 4634 4666 6147 6179 2081 Size: 32 2521 32 1481 32 2467 Fragments arranged by size: 2521 2467 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6565 PsiI TTA'TAA Cuts at: 4135 4135 Size: 6565 PstI C_TGCA'G Cuts at: 830 2448 3776 3917 830 Size: 1618 1328 141 3478 Fragments arranged by size: 3478 1618 1328 141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6565 SalI G'TCGA_C Cuts at: 1936 3907 1936 Size: 1971 4594 SapI GCTCTTCn'nnn_ Cuts at: 3487 3487 Size: 6565 SbfI CC_TGCA'GG Cuts at: 3917 3917 Size: 6565 ScaI AGT'ACT Cuts at: 4813 6525 4813 Size: 1712 4853 SfcI C'TryA_G Cuts at: 826 1045 2444 3772 3913 5048 5729 5920 826 Size: 219 1399 1328 141 1135 681 191 1471 Fragments arranged by size: 1471 1399 1328 1135 681 219 191 141 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6565 SmaI CCC'GGG Cuts at: 1872 3892 1872 Size: 2020 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 2890 4669 5570 5832 820 Size: 197 926 273 674 1779 901 262 1553 Fragments arranged by size: 1779 1553 926 901 674 273 262 197 SnaBI TAC'GTA Cuts at: 493 3535 4356 493 Size: 3042 821 2702 Fragments arranged by size: 3042 2702 821 SpeI A'CTAG_T Cuts at: 152 152 Size: 6565 SphI G_CATG'C Cuts at: 1132 3923 1132 Size: 2791 3774 SspI AAT'ATT Cuts at: 5 52 2674 4489 5 Size: 47 2622 1815 2081 Fragments arranged by size: 2622 2081 1815 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6565 StyI C'CwwG_G Cuts at: 513 1065 2004 2939 3225 4004 513 Size: 552 939 935 286 779 3074 Fragments arranged by size: 3074 939 935 779 552 286 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2795 3071 4592 4609 4762 4947 5392 Size: 1255 1215 276 1521 17 153 185 445 Cuts at: 5392 325 Size: 1498 Fragments arranged by size: 1521 1498 1255 1215 445 276 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3326 4811 6523 Size: 274 80 33 51 1443 1349 1485 1712 Cuts at: 6523 96 Size: 138 Fragments arranged by size: 1712 1485 1443 1349 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3780 3812 6525 6557 3780 Size: 32 2713 32 3788 Fragments arranged by size: 3788 2713 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2411 1316 Size: 1095 5470 XbaI T'CTAG_A Cuts at: 3901 3901 Size: 6565 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3640 3640 Size: 6565 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6565 XmaI C'CCGG_G Cuts at: 1870 3890 1870 Size: 2020 4545 XmnI GAAnn'nnTTC Cuts at: 3418 4694 3418 Size: 1276 5289 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4373 276 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI