(Circular) (Six-base) MAPSORT of: pFN21AA1376.seq Check: 970 from: 1 to: 5947 pFN21AA1376 5947 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3290 1937 Size: 1353 4594 Acc65I G'GTAC_C Cuts at: 2933 3268 2933 Size: 335 5612 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2253 3054 4010 5394 5662 1632 Size: 621 801 956 1384 268 1917 Fragments arranged by size: 1917 1384 956 801 621 268 AflII C'TTAA_G Cuts at: 820 1017 2581 820 Size: 197 1564 4186 Fragments arranged by size: 4186 1564 197 AflIII A'CryG_T Cuts at: 1203 4846 5823 1203 Size: 3643 977 1327 Fragments arranged by size: 3643 1327 977 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5947 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2355 1184 Size: 1171 4776 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2642 2674 2642 Size: 32 5915 AlwNI CAG_nnn'CTG Cuts at: 3690 5262 3690 Size: 1572 4375 ApaLI G'TGCA_C Cuts at: 4004 5160 4004 Size: 1156 4791 ApoI r'AATT_y Cuts at: 1493 2107 2148 2468 2811 3256 3482 3634 5743 Size: 614 41 320 343 445 226 152 2109 Cuts at: 5743 1493 Size: 1697 Fragments arranged by size: 2109 1697 614 445 343 320 226 152 41 AseI AT'TA_AT Cuts at: 160 1032 4501 160 Size: 872 3469 1606 Fragments arranged by size: 3469 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 3272 1870 Size: 73 1329 4545 Fragments arranged by size: 4545 1329 73 BamHI G'GATC_C Cuts at: 3277 3644 3277 Size: 367 5580 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2671 2933 3268 4723 618 Size: 325 217 627 884 262 335 1455 1842 Fragments arranged by size: 1842 1455 884 627 335 325 262 217 BanII G_rGCy'C Cuts at: 1391 3127 3266 1391 Size: 1736 139 4072 Fragments arranged by size: 4072 1736 139 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4976 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4138 4172 1903 Size: 34 2201 34 3678 Fragments arranged by size: 3678 2201 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2080 3839 5049 2080 Size: 1759 1210 2978 Fragments arranged by size: 2978 1759 1210 BclI T'GATC_A Cuts at: 1548 3208 1548 Size: 1660 4287 BfrBI ATG'CAT Cuts at: 2342 2342 Size: 5947 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2397 4558 136 Size: 107 122 71 631 605 725 2161 1525 Fragments arranged by size: 2161 1525 725 631 605 122 107 71 BglII A'GATC_T Cuts at: 5942 5942 Size: 5947 BlpI GC'TnA_GC Cuts at: 3364 3364 Size: 5947 Bme1580I G_kGCm'C Cuts at: 1790 2277 4008 5164 1790 Size: 487 1731 1156 2573 Fragments arranged by size: 2573 1731 1156 487 BmgBI CAC'GTC Cuts at: 1318 2790 1318 Size: 1472 4475 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2130 3678 4636 Size: 416 53 83 399 24 713 1548 958 Cuts at: 4636 442 Size: 1753 Fragments arranged by size: 1753 1548 958 713 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5947 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2671 2671 Size: 5947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2913 4607 1239 Size: 1674 1694 2579 Fragments arranged by size: 2579 1694 1674 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2953 4072 4937 5235 2953 Size: 1119 865 298 3665 Fragments arranged by size: 3665 1119 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4610 882 Size: 617 3111 2219 Fragments arranged by size: 3111 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3738 493 Size: 711 2534 2702 Fragments arranged by size: 2702 2534 711 BsaBI GATnn'nnATC Cuts at: 3201 5941 3201 Size: 2740 3207 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3754 4136 4771 275 Size: 53 83 186 936 2221 382 635 1451 Fragments arranged by size: 2221 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4380 4778 5052 5199 5610 4380 Size: 398 274 147 411 4717 Fragments arranged by size: 4717 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2186 2216 1178 Size: 30 165 30 783 30 4909 Fragments arranged by size: 4909 783 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5947 BseYI C'CCAG_C Cuts at: 2011 3044 5150 5646 2011 Size: 1033 2106 496 2312 Fragments arranged by size: 2312 2106 1033 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5374 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3397 4158 5186 1540 Size: 275 186 1396 761 1028 2301 Fragments arranged by size: 2301 1396 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4008 4093 5164 4008 Size: 85 1071 4791 Fragments arranged by size: 4791 1071 85 BsmI GAATG_Cn' Cuts at: 2342 3458 3551 2342 Size: 1116 93 4738 Fragments arranged by size: 4738 1116 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2098 2780 2098 Size: 682 5265 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 3127 3266 4008 4093 5164 1391 Size: 399 487 850 139 742 85 1071 2174 Fragments arranged by size: 2174 1071 850 742 487 399 139 85 BspEI T'CCGG_A Cuts at: 5610 5610 Size: 5947 BspHI T'CATG_A Cuts at: 3836 5566 3836 Size: 1730 4217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3302 844 Size: 2458 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3834 1394 Size: 429 2011 3507 Fragments arranged by size: 3507 2011 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2068 4442 4616 65 Size: 1183 820 2374 174 1396 Fragments arranged by size: 2374 1396 1183 820 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2198 4591 4778 1700 Size: 110 388 2393 187 2869 Fragments arranged by size: 2869 2393 388 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5947 BssSI C'ACGA_G Cuts at: 3243 4007 5019 3243 Size: 764 1012 4171 Fragments arranged by size: 4171 1012 764 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5947 BstBI TT'CG_AA Cuts at: 2385 4751 2385 Size: 2366 3581 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5947 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5947 BstYI r'GATC_y Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942 Size: 1305 367 385 17 1452 86 12 346 Cuts at: 5942 1972 Size: 1977 Fragments arranged by size: 1977 1452 1305 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2566 2566 Size: 5947 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4456 Fragments arranged by size: 4456 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5208 Fragments arranged by size: 5208 683 56 BtsI GCAGTG_nn' Cuts at: 2407 3463 4257 4277 2407 Size: 1056 794 20 4077 Fragments arranged by size: 4077 1056 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5947 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 3251 3251 Size: 5947 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2063 4954 809 Size: 507 747 2891 1802 Fragments arranged by size: 2891 1802 747 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3394 4283 5912 Size: 54 1425 50 275 18 1564 889 1629 Cuts at: 5912 8 Size: 43 Fragments arranged by size: 1629 1564 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3394 1537 Size: 275 1582 4090 Fragments arranged by size: 4090 1582 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2662 3877 1353 Size: 665 644 1215 3423 Fragments arranged by size: 3423 1215 665 644 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3094 4534 4908 5054 387 Size: 1064 138 1505 1440 374 146 1280 Fragments arranged by size: 1505 1440 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2253 2913 3054 4010 4607 5394 5662 Size: 393 621 660 141 956 597 787 268 Cuts at: 5662 1239 Size: 1524 Fragments arranged by size: 1524 956 787 660 621 597 393 268 141 EcoRI G'AATT_C Cuts at: 3256 3634 3256 Size: 378 5569 EcoRV GAT'ATC Cuts at: 2494 2494 Size: 5947 FspI TGC'GCA Cuts at: 4453 4453 Size: 5947 HaeII r_GCGC'y Cuts at: 1678 5094 1678 Size: 3416 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 3215 3247 4588 Size: 32 135 32 506 32 1105 32 1341 Cuts at: 4588 4620 4662 4694 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1341 1105 506 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3291 3537 669 Size: 988 281 1353 246 3079 Fragments arranged by size: 3079 1353 988 281 246 HindIII A'AGCT_T Cuts at: 748 2038 748 Size: 1290 4657 HpaI GTT'AAC Cuts at: 3537 3537 Size: 5947 KpnI G_GTAC'C Cuts at: 2937 3272 2937 Size: 335 5612 MfeI C'AATT_G Cuts at: 3546 3546 Size: 5947 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5061 5245 1305 Size: 3756 184 2007 Fragments arranged by size: 3756 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4125 Fragments arranged by size: 4125 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2337 2400 2996 3905 4264 4423 518 Size: 648 1171 63 596 909 359 159 2042 Fragments arranged by size: 2042 1171 909 648 596 359 159 63 MspA1I CmG'CkG Cuts at: 1606 3363 4040 5188 5433 1606 Size: 1757 677 1148 245 2120 Fragments arranged by size: 2120 1757 1148 677 245 NaeI GCC'GGC Cuts at: 2200 2200 Size: 5947 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4456 Fragments arranged by size: 4456 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5947 NgoMIV G'CCGG_C Cuts at: 2198 2198 Size: 5947 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5947 NotI GC'GGCC_GC Cuts at: 3394 3394 Size: 5947 NsiI A_TGCA'T Cuts at: 2344 2344 Size: 5947 NspI r_CATG'y Cuts at: 1132 1229 2168 3305 4850 1132 Size: 97 939 1137 1545 2229 Fragments arranged by size: 2229 1545 1137 939 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5947 PciI A'CATG_T Cuts at: 4846 4846 Size: 5947 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5947 PfoI T'CCnGG_A Cuts at: 2890 3153 2890 Size: 263 5684 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 5529 5561 4016 Size: 32 1481 32 4402 Fragments arranged by size: 4402 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5947 PsiI TTA'TAA Cuts at: 3517 3517 Size: 5947 PstI C_TGCA'G Cuts at: 830 3299 830 Size: 2469 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 SalI G'TCGA_C Cuts at: 1936 3289 1936 Size: 1353 4594 SapI GCTCTTCn'nnn_ Cuts at: 2662 2662 Size: 5947 SbfI CC_TGCA'GG Cuts at: 3299 3299 Size: 5947 ScaI AGT'ACT Cuts at: 4195 5907 4195 Size: 1712 4235 SfcI C'TryA_G Cuts at: 826 1045 2842 3295 4430 5111 5302 826 Size: 219 1797 453 1135 681 191 1471 Fragments arranged by size: 1797 1471 1135 681 453 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5947 SmaI CCC'GGG Cuts at: 1872 3274 1872 Size: 1402 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2581 2968 4051 4952 5214 820 Size: 197 926 638 387 1083 901 262 1553 Fragments arranged by size: 1553 1083 926 901 638 387 262 197 SnaBI TAC'GTA Cuts at: 493 3738 493 Size: 3245 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5947 SphI G_CATG'C Cuts at: 1132 3305 1132 Size: 2173 3774 SspI AAT'ATT Cuts at: 5 52 2864 3871 5 Size: 47 2812 1007 2081 Fragments arranged by size: 2812 2081 1007 47 StuI AGG'CCT Cuts at: 1517 2437 2702 1517 Size: 920 265 4762 Fragments arranged by size: 4762 920 265 StyI C'CwwG_G Cuts at: 513 1065 2004 2438 3386 513 Size: 552 939 434 948 3074 Fragments arranged by size: 3074 948 939 552 434 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2404 3058 3974 3991 4144 4329 4774 Size: 1255 824 654 916 17 153 185 445 Cuts at: 4774 325 Size: 1498 Fragments arranged by size: 1498 1255 916 824 654 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4193 5905 96 Size: 274 80 33 51 1443 2216 1712 138 Fragments arranged by size: 2216 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5907 5939 5907 Size: 32 5915 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 2189 3283 2189 Size: 1094 4853 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 3272 1870 Size: 1402 4545 XmnI GAAnn'nnTTC Cuts at: 2625 4076 2625 Size: 1451 4496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI