(Circular) (Six-base) MAPSORT of: pFN21AA1373.seq Check: 7148 from: 1 to: 6088 pFN21AA1373 6088 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3431 1937 Size: 1494 4594 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2354 3154 4151 5535 5803 1632 Size: 722 800 997 1384 268 1917 Fragments arranged by size: 1917 1384 997 800 722 268 AfeI AGC'GCT Cuts at: 2632 2632 Size: 6088 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 4987 5964 1203 Size: 3784 977 1327 Fragments arranged by size: 3784 1327 977 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 2306 2536 3831 5403 2306 Size: 230 1295 1572 2991 Fragments arranged by size: 2991 1572 1295 230 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 2026 2212 2243 2442 2782 2837 2918 3266 Size: 533 186 31 199 340 55 81 348 Cuts at: 3266 3397 3623 3775 5884 1493 Size: 131 226 152 2109 1697 Fragments arranged by size: 2109 1697 533 348 340 226 199 186 152 131 81 55 31 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 2574 3413 1870 Size: 73 631 839 4545 Fragments arranged by size: 4545 839 631 73 BamHI G'GATC_C Cuts at: 3039 3418 3785 3039 Size: 379 367 5342 Fragments arranged by size: 5342 379 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3409 4864 618 Size: 325 217 627 1622 1455 1842 Fragments arranged by size: 1842 1622 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3246 3312 3407 1391 Size: 1855 66 95 4072 Fragments arranged by size: 4072 1855 95 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2142 928 Size: 971 243 4874 Fragments arranged by size: 4874 971 243 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4279 4313 1903 Size: 34 2342 34 3678 Fragments arranged by size: 3678 2342 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4699 136 Size: 107 122 71 631 605 3027 1525 Fragments arranged by size: 3027 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2824 6083 2824 Size: 3259 2829 BlpI GC'TnA_GC Cuts at: 3505 3505 Size: 6088 Bme1580I G_kGCm'C Cuts at: 1790 2101 4149 5305 1790 Size: 311 2048 1156 2573 Fragments arranged by size: 2573 2048 1156 311 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6088 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3819 4777 442 Size: 416 53 83 399 24 2402 958 1753 Fragments arranged by size: 2402 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2724 1056 Size: 1668 4420 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2187 4748 1239 Size: 948 2561 2579 Fragments arranged by size: 2579 2561 948 Bpu10I CC'TnA_GC Cuts at: 2075 2102 2075 Size: 27 6061 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2540 3215 4213 5078 5376 2540 Size: 675 998 865 298 3252 Fragments arranged by size: 3252 998 865 675 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3879 493 Size: 711 2675 2702 Fragments arranged by size: 2702 2675 711 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2189 3895 4277 4912 Size: 53 83 186 936 656 1706 382 635 Cuts at: 4912 275 Size: 1451 Fragments arranged by size: 1706 1451 936 656 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4521 4919 5193 5340 5751 4521 Size: 398 274 147 411 4858 Fragments arranged by size: 4858 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3006 3036 1178 Size: 30 165 30 1603 30 4230 Fragments arranged by size: 4230 1603 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2721 3014 1186 Size: 1535 293 4260 Fragments arranged by size: 4260 1535 293 BseYI C'CCAG_C Cuts at: 5291 5787 5291 Size: 496 5592 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2319 1163 Size: 573 583 4932 Fragments arranged by size: 4932 583 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4149 4234 5305 4149 Size: 85 1071 4932 Fragments arranged by size: 4932 1071 85 BsmI GAATG_Cn' Cuts at: 2345 3599 3692 2345 Size: 1254 93 4741 Fragments arranged by size: 4741 1254 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 3246 3312 3407 4149 4234 5305 Size: 399 311 1145 66 95 742 85 1071 Cuts at: 5305 1391 Size: 2174 Fragments arranged by size: 2174 1145 1071 742 399 311 95 85 66 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 3977 5707 3977 Size: 1730 4358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3443 844 Size: 2599 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3975 1394 Size: 429 2152 3507 Fragments arranged by size: 3507 2152 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2300 3126 4583 4757 65 Size: 1183 1052 826 1457 174 1396 Fragments arranged by size: 1457 1396 1183 1052 826 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4732 4919 1700 Size: 110 2922 187 2869 Fragments arranged by size: 2922 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 4148 5160 4148 Size: 1012 5076 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6088 BstBI TT'CG_AA Cuts at: 2544 4892 2544 Size: 2348 3740 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstYI r'GATC_y Cuts at: 1972 2824 3039 3354 3418 3785 4170 4187 5639 Size: 852 215 315 64 367 385 17 1452 Cuts at: 5639 5725 5737 6083 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 852 385 367 346 315 215 86 64 17 12 Bsu36I CC'TnA_GG Cuts at: 2602 2602 Size: 6088 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4597 Fragments arranged by size: 4597 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2818 487 Size: 683 56 1592 3757 Fragments arranged by size: 3757 1592 683 56 BtsI GCAGTG_nn' Cuts at: 3291 3604 4398 4418 3291 Size: 313 794 20 4961 Fragments arranged by size: 4961 794 313 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 2382 3392 2382 Size: 1010 5078 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053 Size: 54 1425 50 275 18 1705 889 1629 Cuts at: 6053 8 Size: 43 Fragments arranged by size: 1705 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2079 4018 1353 Size: 726 1939 3423 Fragments arranged by size: 3423 1939 726 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2187 2354 3154 4151 4748 5535 5803 Size: 393 555 167 800 997 597 787 268 Cuts at: 5803 1239 Size: 1524 Fragments arranged by size: 1524 997 800 787 597 555 393 268 167 EcoO109I rG'GnC_Cy Cuts at: 2745 2745 Size: 6088 EcoRI G'AATT_C Cuts at: 2026 3397 3775 2026 Size: 1371 378 4339 Fragments arranged by size: 4339 1371 378 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 2634 5235 1678 Size: 956 2601 2531 Fragments arranged by size: 2601 2531 956 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3330 3362 4729 4761 4803 Size: 32 135 32 1758 32 1367 32 42 Cuts at: 4803 4835 1373 Size: 32 2626 Fragments arranged by size: 2626 1758 1367 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3432 3678 669 Size: 988 281 1494 246 3079 Fragments arranged by size: 3079 1494 988 281 246 HindIII A'AGCT_T Cuts at: 748 2108 2267 748 Size: 1360 159 4569 Fragments arranged by size: 4569 1360 159 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4266 Fragments arranged by size: 4266 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2064 4046 4405 4564 518 Size: 648 898 1982 359 159 2042 Fragments arranged by size: 2042 1982 898 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2759 3504 4181 5329 5574 1606 Size: 1153 745 677 1148 245 2120 Fragments arranged by size: 2120 1153 1148 745 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4597 Fragments arranged by size: 4597 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NheI G'CTAG_C Cuts at: 1052 2720 1052 Size: 1668 4420 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 3193 3446 4991 1132 Size: 97 1964 253 1545 2229 Fragments arranged by size: 2229 1964 1545 253 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6088 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 2238 2249 3658 2238 Size: 11 1409 4668 Fragments arranged by size: 4668 1409 11 PstI C_TGCA'G Cuts at: 830 3440 830 Size: 2610 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 PvuII CAG'CTG Cuts at: 2759 2759 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SapI GCTCTTCn'nnn_ Cuts at: 2079 2079 Size: 6088 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 3436 4571 5252 5443 826 Size: 219 2391 1135 681 191 1471 Fragments arranged by size: 2391 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 3194 4192 5093 5355 820 Size: 197 926 612 639 998 901 262 1553 Fragments arranged by size: 1553 998 926 901 639 612 262 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3193 3446 1132 Size: 2061 253 3774 Fragments arranged by size: 3774 2061 253 SspI AAT'ATT Cuts at: 5 52 2411 4012 5 Size: 47 2359 1601 2081 Fragments arranged by size: 2359 2081 1601 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2004 2351 3237 3527 513 Size: 552 939 347 886 290 3074 Fragments arranged by size: 3074 939 886 552 347 290 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4115 4132 4285 4470 4915 325 Size: 1255 2535 17 153 185 445 1498 Fragments arranged by size: 2535 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4334 6046 96 Size: 274 80 33 51 1443 2357 1712 138 Fragments arranged by size: 2357 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6048 6080 6048 Size: 32 6056 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6088 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 2268 4217 2268 Size: 1949 4139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI