Restriction map for
pFN21AA1373
(Linear) (Six-base) MAPSORT of: KIAA1373.seq Check: 850 from: 1 to: 1383
KIAA1373.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 1149 1383
Size: 349 800 234
Fragments arranged by size:
800 349 234
AfeI AGC'GCT
Cuts at: 0 627 1383
Size: 627 756
AlwNI CAG_nnn'CTG
Cuts at: 0 301 531 1383
Size: 301 230 852
Fragments arranged by size:
852 301 230
ApoI r'AATT_y
Cuts at: 0 21 207 238 437 777 832 913 1261
Size: 21 186 31 199 340 55 81 348
Cuts at: 1261 1383
Size: 122
Fragments arranged by size:
348 340 199 186 122 81 55 31
21
AvaI C'yCGr_G
Cuts at: 0 569 1383
Size: 569 814
BamHI G'GATC_C
Cuts at: 0 1034 1383
Size: 1034 349
BanII G_rGCy'C
Cuts at: 0 1241 1307 1383
Size: 1241 66 76
Fragments arranged by size:
1241 76 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 137 1383
Size: 137 1246
BglII A'GATC_T
Cuts at: 0 819 1383
Size: 819 564
Bme1580I G_kGCm'C
Cuts at: 0 96 1383
Size: 96 1287
BmtI G_CTAG'C
Cuts at: 0 719 1383
Size: 719 664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 1383
Size: 182 1201
Bpu10I CC'TnA_GC
Cuts at: 0 70 97 1383
Size: 70 27 1286
Fragments arranged by size:
1286 70 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 1210 1383
Size: 535 675 173
Fragments arranged by size:
675 535 173
BsaHI Gr'CG_yC
Cuts at: 0 184 1383
Size: 184 1199
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1001 1031 1383
Size: 1001 30 352
Fragments arranged by size:
1001 352 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 716 1009 1383
Size: 716 293 374
Fragments arranged by size:
716 374 293
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 1383
Size: 314 1069
BsmI GAATG_Cn'
Cuts at: 0 340 1383
Size: 340 1043
Bsp1286I G_dGCh'C
Cuts at: 0 96 1241 1307 1383
Size: 96 1145 66 76
Fragments arranged by size:
1145 96 76 66
BsrDI GCAATG_nn'
Cuts at: 0 295 1121 1383
Size: 295 826 262
Fragments arranged by size:
826 295 262
BstBI TT'CG_AA
Cuts at: 0 539 1383
Size: 539 844
BstYI r'GATC_y
Cuts at: 0 819 1034 1349 1383
Size: 819 215 315 34
Fragments arranged by size:
819 315 215 34
Bsu36I CC'TnA_GG
Cuts at: 0 597 1383
Size: 597 786
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 813 1383
Size: 813 570
BtsI GCAGTG_nn'
Cuts at: 0 1286 1383
Size: 1286 97
DraI TTT'AAA
Cuts at: 0 377 1383
Size: 377 1006
EarI CTCTTCn'nnn_
Cuts at: 0 74 1383
Size: 74 1309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 349 1149 1383
Size: 182 167 800 234
Fragments arranged by size:
800 234 182 167
EcoO109I rG'GnC_Cy
Cuts at: 0 740 1383
Size: 740 643
EcoRI G'AATT_C
Cuts at: 0 21 1383
Size: 21 1362
HaeII r_GCGC'y
Cuts at: 0 629 1383
Size: 629 754
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1325 1357 1383
Size: 1325 32 26
Fragments arranged by size:
1325 32 26
HindIII A'AGCT_T
Cuts at: 0 103 262 1383
Size: 103 159 1121
Fragments arranged by size:
1121 159 103
MslI CAynn'nnrTG
Cuts at: 0 59 1383
Size: 59 1324
MspA1I CmG'CkG
Cuts at: 0 754 1383
Size: 754 629
NheI G'CTAG_C
Cuts at: 0 715 1383
Size: 715 668
NspI r_CATG'y
Cuts at: 0 1188 1383
Size: 1188 195
PsiI TTA'TAA
Cuts at: 0 233 244 1383
Size: 233 11 1139
Fragments arranged by size:
1139 233 11
PvuII CAG'CTG
Cuts at: 0 754 1383
Size: 754 629
SapI GCTCTTCn'nnn_
Cuts at: 0 74 1383
Size: 74 1309
SmlI C'TyrA_G
Cuts at: 0 550 1189 1383
Size: 550 639 194
Fragments arranged by size:
639 550 194
SphI G_CATG'C
Cuts at: 0 1188 1383
Size: 1188 195
SspI AAT'ATT
Cuts at: 0 406 1383
Size: 406 977
StyI C'CwwG_G
Cuts at: 0 346 1232 1383
Size: 346 886 151
Fragments arranged by size:
886 346 151
XmnI GAAnn'nnTTC
Cuts at: 0 263 1383
Size: 263 1120
Enzymes that do cut:
AcuI AfeI AlwNI ApoI AvaI BamHI BanII BbsI
BglII Bme1580I BmtI BpmI Bpu10I BpuEI BsaHI BsaXI
BseRI BsgI BsmI Bsp1286I BsrDI BstBI BstYI Bsu36I
BtgZI BtsI DraI EarI Eco57MI EcoO109I EcoRI HaeII
Hin4I HindIII MslI MspA1I NheI NspI PsiI PvuII
SapI SmlI SphI SspI StyI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmrI BplI BsaI
BsaAI BsaBI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI