Restriction map for
pFN21AA1346
(Circular) (Six-base) MAPSORT of: pFN21AA1346.seq Check: 6268 from: 1 to: 7606
pFN21AA1346 7606 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5416 278
Size: 53 83 186 936 3880 2468
Fragments arranged by size:
3880 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4949 1937
Size: 3012 4594
Acc65I G'GTAC_C
Cuts at: 4927 4927
Size: 7606
AclI AA'CG_TT
Cuts at: 1563 5733 6106 1563
Size: 4170 373 3063
Fragments arranged by size:
4170 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5669 7053 7321 1632
Size: 4037 1384 268 1917
Fragments arranged by size:
4037 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7409
AflIII A'CryG_T
Cuts at: 1203 3101 3219 3518 6505 7482 1203
Size: 1898 118 299 2987 977 1327
Fragments arranged by size:
2987 1898 1327 977 299 118
AgeI A'CCGG_T
Cuts at: 6437 6437
Size: 7606
AhdI GACnn_n'nnGTC
Cuts at: 6335 6335
Size: 7606
AleI CACnn'nnGTG
Cuts at: 1166 2829 1166
Size: 1663 5943
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3827 1184
Size: 2643 4963
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 7574
AlwNI CAG_nnn'CTG
Cuts at: 2257 2427 2485 2738 3156 3825 5349 6921 2257
Size: 170 58 253 418 669 1524 1572 2942
Fragments arranged by size:
2942 1572 1524 669 418 253 170 58
ApaI G_GGCC'C
Cuts at: 2100 2100
Size: 7606
ApaLI G'TGCA_C
Cuts at: 5663 6819 5663
Size: 1156 6450
ApoI r'AATT_y
Cuts at: 1493 2819 4434 4915 5141 5293 7402 1493
Size: 1326 1615 481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1615 1326 481 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4656 6160 160
Size: 872 3624 1504 1606
Fragments arranged by size:
3624 1606 1504 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7606
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2153 2209 4931 1870
Size: 73 78 132 56 2722 4545
Fragments arranged by size:
4545 2722 132 78 73 56
BamHI G'GATC_C
Cuts at: 4283 4936 5303 4283
Size: 653 367 6586
Fragments arranged by size:
6586 653 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2157 2188 2293 2384 2444
Size: 325 217 627 370 31 105 91 60
Cuts at: 2444 2539 3440 3542 3596 4927 6382 618
Size: 95 901 102 54 1331 1455 1842
Fragments arranged by size:
1842 1455 1331 901 627 370 325 217
105 102 95 91 60 54 31
BanII G_rGCy'C
Cuts at: 1391 2077 2100 2619 4464 4925 1391
Size: 686 23 519 1845 461 4072
Fragments arranged by size:
4072 1845 686 519 461 23
BbeI G_GCGC'C
Cuts at: 2161 2297 2448 2161
Size: 136 151 7319
Fragments arranged by size:
7319 151 136
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2652 4639 928
Size: 971 753 1987 3895
Fragments arranged by size:
3895 1987 971 753
BbvCI CC'TCA_GC
Cuts at: 2420 2420
Size: 7606
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2790 2824 5797 5831 1903
Size: 34 50 34 769 34 2973 34 3678
Fragments arranged by size:
3678 2973 769 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5498 6708 5498
Size: 1210 6396
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7606
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6217 136
Size: 107 122 71 631 605 4545 1525
Fragments arranged by size:
4545 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2928 7601 2928
Size: 4673 2933
BlpI GC'TnA_GC
Cuts at: 5023 5023
Size: 7606
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2100 5667 6823 1790
Size: 232 78 3567 1156 2573
Fragments arranged by size:
3567 2573 1156 232 78
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7606
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2637 3024 3764
Size: 416 53 83 399 24 1220 387 740
Cuts at: 3764 4729 5337 6295 442
Size: 965 608 958 1753
Fragments arranged by size:
1753 1220 965 958 740 608 416 399
387 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2285 2294 4263 6266 1239
Size: 980 66 9 1969 2003 2579
Fragments arranged by size:
2579 2003 1969 980 66 9
Bpu10I CC'TnA_GC
Cuts at: 2420 2420
Size: 7606
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3854 4420 5731 6596 6894 3854
Size: 566 1311 865 298 4566
Fragments arranged by size:
4566 1311 865 566 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2088 2633 3339 6269 882
Size: 617 589 545 706 2930 2219
Fragments arranged by size:
2930 2219 706 617 589 545
BsaAI yAC'GTr
Cuts at: 493 1204 2595 3220 5397 493
Size: 711 1391 625 2177 2702
Fragments arranged by size:
2702 2177 1391 711 625
BsaBI GATnn'nnATC
Cuts at: 7600 7600
Size: 7606
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2158 2294 2342 2445
Size: 53 83 186 936 625 136 48 103
Cuts at: 2445 3296 3957 5413 5795 6430 275
Size: 851 661 1456 382 635 1451
Fragments arranged by size:
1456 1451 936 851 661 635 625 382
186 136 103 83 53 48
BsaWI w'CCGG_w
Cuts at: 2350 6039 6437 6711 6858 7269 2350
Size: 3689 398 274 147 411 2687
Fragments arranged by size:
3689 2687 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4397 4427 1178
Size: 30 165 30 2994 30 4357
Fragments arranged by size:
4357 2994 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 2192 3750 4405 1186
Size: 997 9 1558 655 4387
Fragments arranged by size:
4387 1558 997 655 9
BseYI C'CCAG_C
Cuts at: 2044 2402 2458 2891 3687 4744 6809 7305 2044
Size: 358 56 433 796 1057 2065 496 2345
Fragments arranged by size:
2345 2065 1057 796 496 433 358 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 4737 1163
Size: 573 486 2515 4032
Fragments arranged by size:
4032 2515 573 486
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2624 5056 5817 6845 1540
Size: 275 186 623 2432 761 1028 2301
Fragments arranged by size:
2432 2301 1028 761 623 275 186
BsiHKAI G_wGCw'C
Cuts at: 3051 5667 5752 6823 3051
Size: 2616 85 1071 3834
Fragments arranged by size:
3834 2616 1071 85
BsiWI C'GTAC_G
Cuts at: 3447 3447
Size: 7606
BsmI GAATG_Cn'
Cuts at: 2895 4649 4904 5117 5210 2895
Size: 1754 255 213 93 5291
Fragments arranged by size:
5291 1754 255 213 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2333 3964 2333
Size: 1631 5975
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2077 2100 2619 3051 4464 4925
Size: 399 232 55 23 519 432 1413 461
Cuts at: 4925 5667 5752 6823 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1413 1071 742 519 461 432 399
232 85 55 23
BspEI T'CCGG_A
Cuts at: 2350 7269 2350
Size: 4919 2687
BspHI T'CATG_A
Cuts at: 4219 4837 5495 7225 4219
Size: 618 658 1730 4600
Fragments arranged by size:
4600 1730 658 618
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2463 4961 844
Size: 1619 2498 3489
Fragments arranged by size:
3489 2498 1619
BsrBI CCG'CTC
Cuts at: 1394 1823 2071 5493 1394
Size: 429 248 3422 3507
Fragments arranged by size:
3507 3422 429 248
BsrDI GCAATG_nn'
Cuts at: 65 1248 4498 6101 6275 65
Size: 1183 3250 1603 174 1396
Fragments arranged by size:
3250 1603 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2536 3459 3539 6250 6437 1700
Size: 110 726 923 80 2711 187 2869
Fragments arranged by size:
2869 2711 923 726 187 110 80
BsrGI T'GTAC_A
Cuts at: 96 2880 4383 96
Size: 2784 1503 3319
Fragments arranged by size:
3319 2784 1503
BssHII G'CGCG_C
Cuts at: 1925 2203 1925
Size: 278 7328
BssSI C'ACGA_G
Cuts at: 5666 6678 5666
Size: 1012 6594
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3156 794
Size: 2362 5244
BstBI TT'CG_AA
Cuts at: 6410 6410
Size: 7606
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7606
BstXI CCAn_nnnn'nTGG
Cuts at: 2797 2831 2797
Size: 34 7572
BstYI r'GATC_y
Cuts at: 1972 2260 2928 3821 4166 4283 4936 5303 5688
Size: 288 668 893 345 117 653 367 385
Cuts at: 5688 5705 7157 7243 7255 7601 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 893 668 653 385 367 346
345 288 117 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2124 2824 3584 513
Size: 552 1059 700 760 4535
Fragments arranged by size:
4535 1059 760 700 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6867
Fragments arranged by size:
6867 683 56
BtsI GCAGTG_nn'
Cuts at: 2368 2708 2836 3257 3349 3470 3496 4909 5122
Size: 340 128 421 92 121 26 1413 213
Cuts at: 5122 5916 5936 2368
Size: 794 20 4038
Fragments arranged by size:
4038 1413 794 421 340 213 128 121
92 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7606
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7571
DraI TTT'AAA
Cuts at: 4857 4910 4857
Size: 53 7553
DraIII CAC_nnn'GTG
Cuts at: 3032 3531 3032
Size: 499 7107
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3770 6613 809
Size: 507 2454 2843 1802
Fragments arranged by size:
2843 2454 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2621 5053
Size: 54 1425 50 275 18 306 485 2432
Cuts at: 5053 5942 7571 8
Size: 889 1629 43
Fragments arranged by size:
2432 1629 1425 889 485 306 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2621 5053 1537
Size: 275 809 2432 4090
Fragments arranged by size:
4090 2432 809 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4581 5536 1353
Size: 3228 955 3423
Fragments arranged by size:
3423 3228 955
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2222 2581 3605 6193 6567 6713
Size: 1064 138 633 359 1024 2588 374 146
Cuts at: 6713 387
Size: 1280
Fragments arranged by size:
2588 1280 1064 1024 633 374 359 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2285 2294 4263 5669 6266 7053
Size: 393 587 66 9 1969 1406 597 787
Cuts at: 7053 7321 1239
Size: 268 1524
Fragments arranged by size:
1969 1524 1406 787 597 587 393 268
66 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2429 2429
Size: 7606
EcoO109I rG'GnC_Cy
Cuts at: 2681 2701 3093 3701 2681
Size: 20 392 608 6586
Fragments arranged by size:
6586 608 392 20
EcoRI G'AATT_C
Cuts at: 2819 4434 4915 5293 2819
Size: 1615 481 378 5132
Fragments arranged by size:
5132 1615 481 378
EcoRV GAT'ATC
Cuts at: 4217 4225 4217
Size: 8 7598
FspI TGC'GCA
Cuts at: 6112 6112
Size: 7606
HaeII r_GCGC'y
Cuts at: 1678 2132 2161 2297 2448 2506 6753 1678
Size: 454 29 136 151 58 4247 2531
Fragments arranged by size:
4247 2531 454 151 136 58 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2390 2422 4052 4084 4742
Size: 32 135 32 818 32 1630 32 658
Cuts at: 4742 4774 4834 4866 6247 6279 6321 6353 1373
Size: 32 60 32 1381 32 42 32 2626
Fragments arranged by size:
2626 1630 1381 818 658 135 60 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4950 5196 669
Size: 988 281 3012 246 3079
Fragments arranged by size:
3079 3012 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3906 4798 748
Size: 3158 892 3556
Fragments arranged by size:
3556 3158 892
HpaI GTT'AAC
Cuts at: 5196 5196
Size: 7606
KasI G'GCGC_C
Cuts at: 2157 2293 2444 2157
Size: 136 151 7319
Fragments arranged by size:
7319 151 136
KpnI G_GTAC'C
Cuts at: 4931 4931
Size: 7606
MfeI C'AATT_G
Cuts at: 5205 5205
Size: 7606
MluI A'CGCG_T
Cuts at: 7482 7482
Size: 7606
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 3109 3333 4262 6720 6904 1305
Size: 819 985 224 929 2458 184 2007
Fragments arranged by size:
2458 2007 985 929 819 224 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5784
Fragments arranged by size:
5784 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2778 2795 2829 3240 4259 5564 5923
Size: 648 1612 17 34 411 1019 1305 359
Cuts at: 5923 6082 518
Size: 159 2042
Fragments arranged by size:
2042 1612 1305 1019 648 411 359 159
34 17
MspA1I CmG'CkG
Cuts at: 1606 2126 2257 2485 3603 4270 4410 4744 5022
Size: 520 131 228 1118 667 140 334 278
Cuts at: 5022 5699 6847 7092 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1118 677 667 520 334 278
245 228 140 131
NaeI GCC'GGC
Cuts at: 2538 2538
Size: 7606
NarI GG'CG_CC
Cuts at: 2158 2294 2445 2158
Size: 136 151 7319
Fragments arranged by size:
7319 151 136
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7054
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7606
NgoMIV G'CCGG_C
Cuts at: 2536 2536
Size: 7606
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7606
NotI GC'GGCC_GC
Cuts at: 5053 5053
Size: 7606
NspI r_CATG'y
Cuts at: 1132 1229 3105 3232 3522 4964 6509 1132
Size: 97 1876 127 290 1442 1545 2229
Fragments arranged by size:
2229 1876 1545 1442 290 127 97
PasI CC'CwG_GG
Cuts at: 1348 2522 1348
Size: 1174 6432
PciI A'CATG_T
Cuts at: 3101 3518 6505 3101
Size: 417 2987 4202
Fragments arranged by size:
4202 2987 417
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 2317 2812 3292 3782 1108
Size: 1149 60 495 480 490 4932
Fragments arranged by size:
4932 1149 495 490 480 60
PfoI T'CCnGG_A
Cuts at: 4286 4286
Size: 7606
PmeI GTTT'AAAC
Cuts at: 4910 4910
Size: 7606
PmlI CAC'GTG
Cuts at: 2595 3220 2595
Size: 625 6981
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5675 5707 7188 7220 5675
Size: 32 1481 32 6061
Fragments arranged by size:
6061 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2701 3093 2701
Size: 392 7214
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7606
PsiI TTA'TAA
Cuts at: 5176 5176
Size: 7606
PspOMI G'GGCC_C
Cuts at: 2096 2096
Size: 7606
PstI C_TGCA'G
Cuts at: 830 4958 830
Size: 4128 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7606
PvuII CAG'CTG
Cuts at: 2257 3603 4744 2257
Size: 1346 1141 5119
Fragments arranged by size:
5119 1346 1141
SacII CC_GC'GG
Cuts at: 2127 2127
Size: 7606
SalI G'TCGA_C
Cuts at: 1936 4948 1936
Size: 3012 4594
SanDI GG'GwC_CC
Cuts at: 3093 3093
Size: 7606
SbfI CC_TGCA'GG
Cuts at: 4958 4958
Size: 7606
ScaI AGT'ACT
Cuts at: 3794 5854 7566 3794
Size: 2060 1712 3834
Fragments arranged by size:
3834 2060 1712
SfcI C'TryA_G
Cuts at: 826 1045 2546 3817 4954 6089 6770 6961 826
Size: 219 1501 1271 1137 1135 681 191 1471
Fragments arranged by size:
1501 1471 1271 1137 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2159 2295 2446 2159
Size: 136 151 7319
Fragments arranged by size:
7319 151 136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7606
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7606
SmaI CCC'GGG
Cuts at: 1872 2211 4933 1872
Size: 339 2722 4545
Fragments arranged by size:
4545 2722 339
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3833 4399 5710 6611 6873 820
Size: 197 926 1890 566 1311 901 262 1553
Fragments arranged by size:
1890 1553 1311 926 901 566 262 197
SnaBI TAC'GTA
Cuts at: 493 5397 493
Size: 4904 2702
SpeI A'CTAG_T
Cuts at: 152 4199 152
Size: 4047 3559
SphI G_CATG'C
Cuts at: 1132 4964 1132
Size: 3832 3774
SspI AAT'ATT
Cuts at: 5 52 5530 5
Size: 47 5478 2081
Fragments arranged by size:
5478 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7606
StyI C'CwwG_G
Cuts at: 513 1065 3329 3704 4029 5045 513
Size: 552 2264 375 325 1016 3074
Fragments arranged by size:
3074 2264 1016 552 375 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2657 3043 5633 5650 5803 5988 6433
Size: 1255 1077 386 2590 17 153 185 445
Cuts at: 6433 325
Size: 1498
Fragments arranged by size:
2590 1498 1255 1077 445 386 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2880 3744 3792
Size: 274 80 33 51 1443 903 864 48
Cuts at: 3792 4383 5852 7564 96
Size: 591 1469 1712 138
Fragments arranged by size:
1712 1469 1443 903 864 591 274 138
80 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2535 2567 3781 3813 7566 7598 2535
Size: 32 1214 32 3753 32 2543
Fragments arranged by size:
3753 2543 1214 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7606
XbaI T'CTAG_A
Cuts at: 4942 4942
Size: 7606
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3476 3476
Size: 7606
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7606
XmaI C'CCGG_G
Cuts at: 1870 2209 4931 1870
Size: 339 2722 4545
Fragments arranged by size:
4545 2722 339
XmnI GAAnn'nnTTC
Cuts at: 3939 4434 5735 3939
Size: 495 1301 5810
Fragments arranged by size:
5810 1301 495
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5414 276
Size: 53 83 186 936 3880 2468
Fragments arranged by size:
3880 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BstZ17I
Bsu36I EcoICRI FalI FseI FspAI NruI NsiI PacI
PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI
SwaI