Restriction map for
pFN21AA1282
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspI BamHI BbrPI
BclI BfrI BsaAI BsaBI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HpaI KpnI MamI
MfeI MluI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SfuI SgrAI SnaBI SpeI
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 127 GTCTAC GT^[AC][GT]AC
AccIII 629 TCCGGA T^CCGGA
AcsI 834 GAATTT [GA]^AATT[CT]
AgeI 1049 ACCGGT A^CCGGT
AocI 3011 CCTCAGG CC^T[ACGT]AGG
Aor51HI 939 AGCGCT AGC^GCT
ApoI 834 GAATTT [GA]^AATT[CT]
AviII 1343 TGCGCA TGC^GCA
AvrII 1914 CCTAGG C^CTAGG
BalI 1143 TGGCCA TGG^CCA
BciVI 1586 GTATCC GTATCC(6/5)
BlnI 1914 CCTAGG C^CTAGG
BseAI 629 TCCGGA T^CCGGA
BsmBI 2942 CGTCTC CGTCTC(1/5)
BspEI 629 TCCGGA T^CCGGA
BssHII 1695 GCGCGC G^CGCGC
BstXI 3201 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3011 CCTCAGG CC^T[ACGT]AGG
DraIII 1477 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2655 GACAGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 939 AGCGCT AGC^GCT
Eco81I 3011 CCTCAGG CC^T[ACGT]AGG
FspI 1343 TGCGCA TGC^GCA
HincII 973 GTCAAC GT[CT]^[GA]AC
HindII 973 GTCAAC GT[CT]^[GA]AC
HindIII 2638 AAGCTT A^AGCTT
MluNI 1143 TGGCCA TGG^CCA
MroI 629 TCCGGA T^CCGGA
MscI 1143 TGGCCA TGG^CCA
PaeR7I 2963 CTCGAG C^TCGAG
PinAI 1049 ACCGGT A^CCGGT
PshAI 2693 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1845 CCTGCAGG CCTGCA^GG
ScaI 1619 AGTACT AGT^ACT
SfiI 2530 GGCCAGTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 167 CCCGGG CCC^GGG
SphI 1610 GCATGC GCATG^C
Sse8387I 1845 CCTGCAGG CCTGCA^GG
TfiI 1168 GAATC G^A[AT]TC
Tsp509I 835 AATT ^AATT
XcmI 701 CCACACTCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2963 CTCGAG C^TCGAG
XmaCI 167 CCCGGG C^CCGGG
XmaI 167 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 977 ACGTGT A^C[GA][CT]GT
1409 ACGTGT
Asp700I 33 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1434 GAAGATCTTC
BbsI 1002 GAAGAC GAAGAC(2/6)
2485 GAAGAC
BglII 1436 AGATCT A^GATCT
2454 AGATCT
BlpI 283 GCTGAGC GC^T[ACGT]AGC
2097 GCTCAGC
Bpu1102I 283 GCTGAGC GC^T[ACGT]AGC
2097 GCTCAGC
BpuAI 1002 GAAGAC GAAGAC(2/6)
2485 GAAGAC
BsaI 3017 GAGACC GGTCTC(1/5)
2220 GGTCTC
CelII 283 GCTGAGC GC^T[ACGT]AGC
2097 GCTCAGC
NaeI 3 GCCGGC GCC^GGC
452 GCCGGC
NgoMIV 3 GCCGGC G^CCGGC
452 GCCGGC
PflMI 2432 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3171 CCACAGCCTGG
PleI 1221 GACTC GAGTC(4/5)
2995 GACTC
SapI 303 GAAGAGC GCTCTTC(1/4)
114 GCTCTTC
SexAI 913 ACCTGGT A^CC[AT]GGT
1232 ACCTGGT
Van91I 2432 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3171 CCACAGCCTGG
XmnI 33 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1434 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvCI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII