(Circular) (Six-base) MAPSORT of: pFN21AA1282.seq Check: 5142 from: 1 to: 7954 pFN21AA1282 7954 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2553 3729 3955 4666 2553 Size: 1176 226 711 5841 Fragments arranged by size: 5841 1176 711 226 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5764 278 Size: 53 83 186 936 4228 2468 Fragments arranged by size: 4228 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2133 5297 1937 Size: 196 3164 4594 Fragments arranged by size: 4594 3164 196 Acc65I G'GTAC_C Cuts at: 5275 5275 Size: 7954 AclI AA'CG_TT Cuts at: 1563 6081 6454 1563 Size: 4518 373 3063 Fragments arranged by size: 4518 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3026 4063 6017 7401 7669 1632 Size: 1394 1037 1954 1384 268 1917 Fragments arranged by size: 1954 1917 1394 1384 1037 268 AfeI AGC'GCT Cuts at: 2946 2946 Size: 7954 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7757 AflIII A'CryG_T Cuts at: 1203 2982 3414 6853 7830 1203 Size: 1779 432 3439 977 1327 Fragments arranged by size: 3439 1779 1327 977 432 AgeI A'CCGG_T Cuts at: 3054 6785 3054 Size: 3731 4223 AhdI GACnn_n'nnGTC Cuts at: 6683 6683 Size: 7954 AleI CACnn'nnGTG Cuts at: 1166 2855 3398 1166 Size: 1689 543 5722 Fragments arranged by size: 5722 1689 543 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7954 AlwNI CAG_nnn'CTG Cuts at: 2793 3208 4021 4681 4816 5099 5697 7269 2793 Size: 415 813 660 135 283 598 1572 3478 Fragments arranged by size: 3478 1572 813 660 598 415 283 135 ApaI G_GGCC'C Cuts at: 2429 2998 4220 4414 4611 5091 2429 Size: 569 1222 194 197 480 5292 Fragments arranged by size: 5292 1222 569 480 197 194 ApaLI G'TGCA_C Cuts at: 6011 7167 6011 Size: 1156 6798 ApoI r'AATT_y Cuts at: 1493 2839 5263 5489 5641 7750 1493 Size: 1346 2424 226 152 2109 1697 Fragments arranged by size: 2424 2109 1697 1346 226 152 AseI AT'TA_AT Cuts at: 160 1032 6508 160 Size: 872 5476 1606 Fragments arranged by size: 5476 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7954 AvaI C'yCGr_G Cuts at: 1870 1943 2172 4061 4761 4968 5279 1870 Size: 73 229 1889 700 207 311 4545 Fragments arranged by size: 4545 1889 700 311 229 207 73 AvrII C'CTAG_G Cuts at: 3919 3919 Size: 7954 BamHI G'GATC_C Cuts at: 5284 5651 5284 Size: 367 7587 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2460 2541 3470 3902 Size: 325 217 627 242 431 81 929 432 Cuts at: 3902 4735 4921 5275 6730 618 Size: 833 186 354 1455 1842 Fragments arranged by size: 1842 1455 929 833 627 432 431 354 325 242 217 186 81 BanII G_rGCy'C Cuts at: 1391 2121 2238 2319 2429 2766 2998 4104 4220 Size: 730 117 81 110 337 232 1106 116 Cuts at: 4220 4251 4414 4469 4611 4958 5010 5040 5091 Size: 31 163 55 142 347 52 30 51 Cuts at: 5091 5136 5273 1391 Size: 45 137 4072 Fragments arranged by size: 4072 1106 730 347 337 232 163 142 137 117 116 110 81 55 52 51 45 31 30 BbeI G_GCGC'C Cuts at: 2033 2464 3474 4739 2033 Size: 431 1010 1265 5248 Fragments arranged by size: 5248 1265 1010 431 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3014 4497 928 Size: 971 1115 1483 4385 Fragments arranged by size: 4385 1483 1115 971 BbvCI CC'TCA_GC Cuts at: 2178 4291 4383 5112 2178 Size: 2113 92 729 5020 Fragments arranged by size: 5020 2113 729 92 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3892 3926 4031 4065 4691 Size: 34 50 34 1871 34 105 34 626 Cuts at: 4691 4725 5233 5267 6145 6179 1903 Size: 34 508 34 878 34 3678 Fragments arranged by size: 3678 1871 878 626 508 105 50 34 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 5846 7056 3602 Size: 2244 1210 4500 Fragments arranged by size: 4500 2244 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7954 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2111 2469 Size: 107 122 71 631 605 337 102 358 Cuts at: 2469 3641 3854 4542 6565 136 Size: 1172 213 688 2023 1525 Fragments arranged by size: 2023 1525 1172 688 631 605 358 337 213 122 107 102 71 BglII A'GATC_T Cuts at: 3441 4459 7949 3441 Size: 1018 3490 3446 Fragments arranged by size: 3490 3446 1018 BlpI GC'TnA_GC Cuts at: 2289 4103 5371 2289 Size: 1814 1268 4872 Fragments arranged by size: 4872 1814 1268 Bme1580I G_kGCm'C Cuts at: 1790 2429 2479 2544 2671 2733 2998 3784 4220 Size: 639 50 65 127 62 265 786 436 Cuts at: 4220 4414 4455 4611 4760 4787 5091 6015 7171 Size: 194 41 156 149 27 304 924 1156 Cuts at: 7171 1790 Size: 2573 Fragments arranged by size: 2573 1156 924 786 639 436 304 265 194 156 149 127 65 62 50 41 27 BmgBI CAC'GTC Cuts at: 1318 3988 4316 1318 Size: 2670 328 4956 Fragments arranged by size: 4956 2670 328 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2760 3130 4839 Size: 416 53 83 399 24 1343 370 1709 Cuts at: 4839 5094 5685 6643 442 Size: 255 591 958 1753 Fragments arranged by size: 1753 1709 1343 958 591 416 399 370 255 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7954 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4118 4118 Size: 7954 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3242 3279 3344 3641 4208 4237 5148 5204 Size: 2003 37 65 297 567 29 911 56 Cuts at: 5204 6614 1239 Size: 1410 2579 Fragments arranged by size: 2579 2003 1410 911 567 297 65 56 37 29 Bpu10I CC'TnA_GC Cuts at: 2178 4157 4291 4383 5112 2178 Size: 1979 134 92 729 5020 Fragments arranged by size: 5020 1979 729 134 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3590 3878 4894 6079 6944 7242 3590 Size: 288 1016 1185 865 298 4302 Fragments arranged by size: 4302 1185 1016 865 298 288 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4231 5016 6617 882 Size: 617 2732 785 1601 2219 Fragments arranged by size: 2732 2219 1601 785 617 BsaAI yAC'GTr Cuts at: 493 1204 5745 493 Size: 711 4541 2702 Fragments arranged by size: 4541 2702 711 BsaBI GATnn'nnATC Cuts at: 7948 7948 Size: 7954 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 2461 3471 4736 Size: 53 83 186 936 497 431 1010 1265 Cuts at: 4736 5761 6143 6778 275 Size: 1025 382 635 1451 Fragments arranged by size: 1451 1265 1025 1010 936 635 497 431 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2304 2634 3054 6387 6785 7059 7206 7617 2304 Size: 330 420 3333 398 274 147 411 2641 Fragments arranged by size: 3333 2641 420 411 398 330 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3350 3380 4136 4166 4739 Size: 30 165 30 1947 30 756 30 573 Cuts at: 4739 4769 1178 Size: 30 4363 Fragments arranged by size: 4363 1947 756 573 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4205 4273 4899 5049 1186 Size: 3019 68 626 150 4091 Fragments arranged by size: 4091 3019 626 150 68 BseYI C'CCAG_C Cuts at: 2095 2786 3331 4121 4231 4237 4610 4786 4858 Size: 691 545 790 110 6 373 176 72 Cuts at: 4858 4942 5039 7157 7653 2095 Size: 84 97 2118 496 2396 Fragments arranged by size: 2396 2118 790 691 545 496 373 176 110 97 84 72 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3439 3707 3796 4022 4261 4742 1163 Size: 573 1703 268 89 226 239 481 4375 Fragments arranged by size: 4375 1703 573 481 268 239 226 89 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5404 6165 7193 1540 Size: 275 186 3403 761 1028 2301 Fragments arranged by size: 3403 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2238 2274 2526 2753 3894 3912 4104 4958 6015 Size: 36 252 227 1141 18 192 854 1057 Cuts at: 6015 6100 7171 2238 Size: 85 1071 3021 Fragments arranged by size: 3021 1141 1071 1057 854 252 227 192 85 36 18 BsmI GAATG_Cn' Cuts at: 5465 5558 5465 Size: 93 7861 BsmBI CGTCTCn'nnnn_ Cuts at: 4953 4953 Size: 7954 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2238 2274 2319 2429 2479 2526 Size: 399 331 117 36 45 110 50 47 Cuts at: 2526 2544 2671 2733 2753 2766 2998 3784 3894 Size: 18 127 62 20 13 232 786 110 Cuts at: 3894 3912 4104 4220 4251 4414 4455 4469 4611 Size: 18 192 116 31 163 41 14 142 Cuts at: 4611 4760 4787 4958 5010 5040 5091 5136 5273 Size: 149 27 171 52 30 51 45 137 Cuts at: 5273 6015 6100 7171 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 786 742 399 331 232 192 171 163 149 142 137 127 117 116 110 110 85 62 52 51 50 47 45 45 41 36 31 30 27 20 18 18 14 13 BspEI T'CCGG_A Cuts at: 2634 7617 2634 Size: 4983 2971 BspHI T'CATG_A Cuts at: 5843 7573 5843 Size: 1730 6224 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2553 2967 3573 3729 3955 4666 5309 844 Size: 1709 414 606 156 226 711 643 3489 Fragments arranged by size: 3489 1709 711 643 606 414 226 156 BsrBI CCG'CTC Cuts at: 1394 1823 2313 4161 4584 5841 1394 Size: 429 490 1848 423 1257 3507 Fragments arranged by size: 3507 1848 1257 490 429 423 BsrDI GCAATG_nn' Cuts at: 65 1248 6449 6623 65 Size: 1183 5201 174 1396 Fragments arranged by size: 5201 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 2457 2502 3054 6598 6785 1700 Size: 110 198 449 45 552 3544 187 2869 Fragments arranged by size: 3544 2869 552 449 198 187 110 45 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7954 BssHII G'CGCG_C Cuts at: 1925 3700 1925 Size: 1775 6179 BssSI C'ACGA_G Cuts at: 6014 7026 6014 Size: 1012 6942 BstAPI GCAn_nnn'nTGC Cuts at: 794 3616 4021 4681 794 Size: 2822 405 660 4067 Fragments arranged by size: 4067 2822 660 405 BstBI TT'CG_AA Cuts at: 6758 6758 Size: 7954 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7954 BstXI CCAn_nnnn'nTGG Cuts at: 5213 5213 Size: 7954 BstYI r'GATC_y Cuts at: 1972 2250 3441 4459 5284 5651 6036 6053 7505 Size: 278 1191 1018 825 367 385 17 1452 Cuts at: 7505 7591 7603 7949 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1191 1018 825 385 367 346 278 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5017 5017 Size: 7954 BtgI C'CryG_G Cuts at: 513 1065 2127 2776 2805 3044 3075 3766 3882 Size: 552 1062 649 29 239 31 691 116 Cuts at: 3882 4221 4406 4442 4603 5206 513 Size: 339 185 36 161 603 3261 Fragments arranged by size: 3261 1062 691 649 603 552 339 239 185 161 116 36 31 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2041 2923 3694 3858 487 Size: 683 56 815 882 771 164 4583 Fragments arranged by size: 4583 882 815 771 683 164 56 BtsI GCAGTG_nn' Cuts at: 3086 4022 5470 6264 6284 3086 Size: 936 1448 794 20 4756 Fragments arranged by size: 4756 1448 936 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7954 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7919 DraI TTT'AAA Cuts at: 5258 5258 Size: 7954 DraIII CAC_nnn'GTG Cuts at: 3487 3487 Size: 7954 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 6961 809 Size: 507 3350 2295 1802 Fragments arranged by size: 3350 2295 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2455 2470 Size: 54 1425 50 275 18 180 445 15 Cuts at: 2470 2802 3096 3148 3274 3507 3759 5401 6290 Size: 332 294 52 126 233 252 1642 889 Cuts at: 6290 7919 8 Size: 1629 43 Fragments arranged by size: 1642 1629 1425 889 445 332 294 275 252 233 180 126 54 52 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 5401 1537 Size: 275 3589 4090 Fragments arranged by size: 4090 3589 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2126 2303 2384 2822 5884 1353 Size: 773 177 81 438 3062 3423 Fragments arranged by size: 3423 3062 773 438 177 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2231 2524 3409 6541 6915 7061 Size: 1064 138 642 293 885 3132 374 146 Cuts at: 7061 387 Size: 1280 Fragments arranged by size: 3132 1280 1064 885 642 374 293 146 138 EcoICRI GAG'CTC Cuts at: 2236 4102 4956 2236 Size: 1866 854 5234 Fragments arranged by size: 5234 1866 854 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3026 3242 3279 3344 3641 4063 4208 Size: 393 1394 216 37 65 297 422 145 Cuts at: 4208 4237 5148 5204 6017 6614 7401 7669 1239 Size: 29 911 56 813 597 787 268 1524 Fragments arranged by size: 1524 1394 911 813 787 597 422 393 297 268 216 145 65 56 37 29 EcoNI CCTnn'n_nnAGG Cuts at: 3725 3923 4338 5016 3725 Size: 198 415 678 6663 Fragments arranged by size: 6663 678 415 198 EcoO109I rG'GnC_Cy Cuts at: 2020 2260 2425 2426 2812 2994 3733 3856 4216 Size: 240 165 1 386 182 739 123 360 Cuts at: 4216 4217 4411 4904 4937 5152 5170 2020 Size: 1 194 493 33 215 18 4804 Fragments arranged by size: 4804 739 493 386 360 240 215 194 182 165 123 33 18 1 1 EcoRI G'AATT_C Cuts at: 5263 5641 5263 Size: 378 7576 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2662 2662 Size: 7954 FspI TGC'GCA Cuts at: 3350 6460 3350 Size: 3110 4844 HaeII r_GCGC'y Cuts at: 1678 2033 2464 2948 3075 3357 3474 4739 7101 Size: 355 431 484 127 282 117 1265 2362 Cuts at: 7101 1678 Size: 2531 Fragments arranged by size: 2531 2362 1265 484 431 355 282 127 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3044 3076 4136 4168 4235 Size: 32 135 32 1472 32 1060 32 67 Cuts at: 4235 4267 5015 5047 6595 6627 6669 6701 1373 Size: 32 748 32 1548 32 42 32 2626 Fragments arranged by size: 2626 1548 1472 1060 748 135 67 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2980 5298 5544 669 Size: 988 281 1042 2318 246 3079 Fragments arranged by size: 3079 2318 1042 988 281 246 HindIII A'AGCT_T Cuts at: 748 4643 748 Size: 3895 4059 HpaI GTT'AAC Cuts at: 5544 5544 Size: 7954 KasI G'GCGC_C Cuts at: 2029 2460 3470 4735 2029 Size: 431 1010 1265 5248 Fragments arranged by size: 5248 1265 1010 431 KpnI G_GTAC'C Cuts at: 5279 5279 Size: 7954 MfeI C'AATT_G Cuts at: 5553 5553 Size: 7954 MluI A'CGCG_T Cuts at: 7830 7830 Size: 7954 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7068 7252 1305 Size: 5763 184 2007 Fragments arranged by size: 5763 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3150 10 Size: 54 1425 343 1318 4814 Fragments arranged by size: 4814 1425 1318 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2855 3098 3398 3713 3887 5912 6271 Size: 648 1689 243 300 315 174 2025 359 Cuts at: 6271 6430 518 Size: 159 2042 Fragments arranged by size: 2042 2025 1689 648 359 315 300 243 174 159 MspA1I CmG'CkG Cuts at: 1606 2191 2778 3046 3154 3257 3742 3768 4027 Size: 585 587 268 108 103 485 26 259 Cuts at: 4027 4241 4295 4862 5370 6047 7195 7440 1606 Size: 214 54 567 508 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 587 585 567 508 485 268 259 245 214 108 103 54 26 NaeI GCC'GGC Cuts at: 2010 2459 2010 Size: 449 7505 NarI GG'CG_CC Cuts at: 2030 2461 3471 4736 2030 Size: 431 1010 1265 5248 Fragments arranged by size: 5248 1265 1010 431 NcoI C'CATG_G Cuts at: 513 1065 3882 4442 4603 5206 513 Size: 552 2817 560 161 603 3261 Fragments arranged by size: 3261 2817 603 560 552 161 NdeI CA'TA_TG Cuts at: 387 387 Size: 7954 NgoMIV G'CCGG_C Cuts at: 2008 2457 2008 Size: 449 7505 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7954 NotI GC'GGCC_GC Cuts at: 5401 5401 Size: 7954 NspI r_CATG'y Cuts at: 1132 1229 2969 3526 3619 5312 6857 1132 Size: 97 1740 557 93 1693 1545 2229 Fragments arranged by size: 2229 1740 1693 1545 557 97 93 PasI CC'CwG_GG Cuts at: 1348 4470 4932 5166 1348 Size: 3122 462 234 4136 Fragments arranged by size: 4136 3122 462 234 PciI A'CATG_T Cuts at: 6853 6853 Size: 7954 PflMI CCAn_nnn'nTGG Cuts at: 1108 4443 5182 1108 Size: 3335 739 3880 Fragments arranged by size: 3880 3335 739 PfoI T'CCnGG_A Cuts at: 2046 2334 2046 Size: 288 7666 PmeI GTTT'AAAC Cuts at: 5258 5258 Size: 7954 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6023 6055 7536 7568 6023 Size: 32 1481 32 6409 Fragments arranged by size: 6409 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2812 3733 4937 5170 2812 Size: 921 1204 233 5596 Fragments arranged by size: 5596 1204 921 233 PshAI GACnn'nnGTC Cuts at: 1613 4702 1613 Size: 3089 4865 PsiI TTA'TAA Cuts at: 5524 5524 Size: 7954 PspOMI G'GGCC_C Cuts at: 2425 2994 4216 4410 4607 5087 2425 Size: 569 1222 194 197 480 5292 Fragments arranged by size: 5292 1222 569 480 197 194 PspXI vC'TCGA_Gb Cuts at: 4968 4968 Size: 7954 PstI C_TGCA'G Cuts at: 830 2550 3204 3293 3681 3741 3855 4017 4026 Size: 1720 654 89 388 60 114 162 9 Cuts at: 4026 4138 4686 4704 4812 4854 5306 830 Size: 112 548 18 108 42 452 3478 Fragments arranged by size: 3478 1720 654 548 452 388 162 114 112 108 89 60 42 18 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7954 PvuII CAG'CTG Cuts at: 3257 3742 4027 4241 4295 4862 3257 Size: 485 285 214 54 567 6349 Fragments arranged by size: 6349 567 485 285 214 54 SacI G_AGCT'C Cuts at: 2238 4104 4958 2238 Size: 1866 854 5234 Fragments arranged by size: 5234 1866 854 SacII CC_GC'GG Cuts at: 2779 3047 3769 2779 Size: 268 722 6964 Fragments arranged by size: 6964 722 268 SalI G'TCGA_C Cuts at: 1936 5296 1936 Size: 3360 4594 SanDI GG'GwC_CC Cuts at: 4937 5170 4937 Size: 233 7721 SapI GCTCTTCn'nnn_ Cuts at: 2126 2303 2126 Size: 177 7777 SbfI CC_TGCA'GG Cuts at: 3855 5306 3855 Size: 1451 6503 ScaI AGT'ACT Cuts at: 3626 6202 7914 3626 Size: 2576 1712 3666 Fragments arranged by size: 3666 2576 1712 SexAI A'CCwGG_T Cuts at: 2918 3237 2918 Size: 319 7635 SfcI C'TryA_G Cuts at: 826 1045 2546 2734 3200 3289 3677 3737 3851 Size: 219 1501 188 466 89 388 60 114 Cuts at: 3851 4013 4022 4134 4682 4700 4808 4850 5302 Size: 162 9 112 548 18 108 42 452 Cuts at: 5302 6437 7118 7309 826 Size: 1135 681 191 1471 Fragments arranged by size: 1501 1471 1135 681 548 466 452 388 219 191 188 162 114 112 108 89 60 42 18 9 SfiI GGCCn_nnn'nGGCC Cuts at: 4542 4542 Size: 7954 SfoI GGC'GCC Cuts at: 2031 2462 3472 4737 2031 Size: 431 1010 1265 5248 Fragments arranged by size: 5248 1265 1010 431 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7954 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7954 SmaI CCC'GGG Cuts at: 1872 2174 5281 1872 Size: 302 3107 4545 Fragments arranged by size: 4545 3107 302 SmlI C'TyrA_G Cuts at: 820 1017 1943 3605 3893 4873 4968 6058 6959 Size: 197 926 1662 288 980 95 1090 901 Cuts at: 6959 7221 820 Size: 262 1553 Fragments arranged by size: 1662 1553 1090 980 926 901 288 262 197 95 SnaBI TAC'GTA Cuts at: 493 5745 493 Size: 5252 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7954 SphI G_CATG'C Cuts at: 1132 3619 5312 1132 Size: 2487 1693 3774 Fragments arranged by size: 3774 2487 1693 SspI AAT'ATT Cuts at: 5 52 5878 5 Size: 47 5826 2081 Fragments arranged by size: 5826 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7954 StyI C'CwwG_G Cuts at: 513 1065 2559 3838 3882 3919 4442 4603 5206 Size: 552 1494 1279 44 37 523 161 603 Cuts at: 5206 5393 513 Size: 187 3074 Fragments arranged by size: 3074 1494 1279 603 552 523 187 161 44 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4106 5981 5998 6151 6336 6781 325 Size: 1255 2526 1875 17 153 185 445 1498 Fragments arranged by size: 2526 1875 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2616 2985 3066 Size: 274 80 33 51 1443 639 369 81 Cuts at: 3066 3624 6200 7912 96 Size: 558 2576 1712 138 Fragments arranged by size: 2576 1712 1443 639 558 369 274 138 81 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4739 4771 7914 7946 4739 Size: 32 3143 32 4747 Fragments arranged by size: 4747 3143 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7954 XbaI T'CTAG_A Cuts at: 5290 5290 Size: 7954 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2713 2713 Size: 7954 XhoI C'TCGA_G Cuts at: 1943 4968 1943 Size: 3025 4929 XmaI C'CCGG_G Cuts at: 1870 2172 5279 1870 Size: 302 3107 4545 Fragments arranged by size: 4545 3107 302 XmnI GAAnn'nnTTC Cuts at: 2042 3443 6083 2042 Size: 1401 2640 3913 Fragments arranged by size: 3913 2640 1401 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5762 276 Size: 53 83 186 936 4228 2468 Fragments arranged by size: 4228 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SrfI SwaI