(Circular) (Six-base) MAPSORT of: pFN21AA1248.seq Check: 9414 from: 1 to: 5776 pFN21AA1248 5776 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3839 5223 5491 1632 Size: 2207 1384 268 1917 Fragments arranged by size: 2207 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 2988 4607 2988 Size: 1619 4157 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 2206 3519 5091 2206 Size: 1313 1572 2891 Fragments arranged by size: 2891 1572 1313 ApaI G_GGCC'C Cuts at: 3048 3048 Size: 5776 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 2368 2590 3085 3311 3463 5572 1493 Size: 875 222 495 226 152 2109 1697 Fragments arranged by size: 2109 1697 875 495 226 222 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 3049 3101 1870 Size: 73 1106 52 4545 Fragments arranged by size: 4545 1106 73 52 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2594 2627 2594 Size: 33 5743 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2129 3097 4552 618 Size: 325 217 627 342 968 1455 1842 Fragments arranged by size: 1842 1455 968 627 342 325 217 BanII G_rGCy'C Cuts at: 1391 2290 2583 3048 3095 1391 Size: 899 293 465 47 4072 Fragments arranged by size: 4072 899 465 293 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4805 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3052 4387 136 Size: 107 122 71 631 605 1380 1335 1525 Fragments arranged by size: 1525 1380 1335 631 605 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 3048 3057 3837 4993 1790 Size: 1258 9 780 1156 2573 Fragments arranged by size: 2573 1258 1156 780 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2445 2445 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2424 4436 1239 Size: 1185 2012 2579 Fragments arranged by size: 2579 2012 1185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2804 3901 4766 5064 2804 Size: 1097 865 298 3516 Fragments arranged by size: 3516 1097 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2679 2742 2949 3073 4439 882 Size: 617 1180 63 207 124 1366 2219 Fragments arranged by size: 2219 1366 1180 617 207 124 63 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2063 3583 3965 4600 Size: 53 83 186 936 530 1520 382 635 Cuts at: 4600 275 Size: 1451 Fragments arranged by size: 1520 1451 936 635 530 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2542 2834 2988 4209 4607 4881 5028 5439 2542 Size: 292 154 1221 398 274 147 411 2879 Fragments arranged by size: 2879 1221 411 398 292 274 154 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2277 2307 2379 2409 1178 Size: 30 165 30 874 30 72 30 4545 Fragments arranged by size: 4545 874 165 72 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 1186 Size: 865 4911 BseYI C'CCAG_C Cuts at: 2149 2847 4979 5475 2149 Size: 698 2132 496 2450 Fragments arranged by size: 2450 2132 698 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2045 2988 1163 Size: 573 309 943 3951 Fragments arranged by size: 3951 943 573 309 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2583 3837 3922 4993 2583 Size: 1254 85 1071 3366 Fragments arranged by size: 3366 1254 1071 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2290 2583 3048 3057 3095 3837 3922 Size: 399 500 293 465 9 38 742 85 Cuts at: 3922 4993 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 500 465 399 293 85 38 9 BspEI T'CCGG_A Cuts at: 2542 2834 5439 2542 Size: 292 2605 2879 Fragments arranged by size: 2879 2605 292 BspHI T'CATG_A Cuts at: 2761 3665 5395 2761 Size: 904 1730 3142 Fragments arranged by size: 3142 1730 904 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3131 844 Size: 2287 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2997 3663 1394 Size: 429 1174 666 3507 Fragments arranged by size: 3507 1174 666 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2988 4420 4607 1700 Size: 110 1178 1432 187 2869 Fragments arranged by size: 2869 1432 1178 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 2023 794 Size: 1229 4547 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstYI r'GATC_y Cuts at: 1972 3106 3473 3858 3875 5327 5413 5425 5771 Size: 1134 367 385 17 1452 86 12 346 Cuts at: 5771 1972 Size: 1977 Fragments arranged by size: 1977 1452 1134 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2067 2720 2067 Size: 653 5123 BtgI C'CryG_G Cuts at: 513 1065 2004 3060 513 Size: 552 939 1056 3229 Fragments arranged by size: 3229 1056 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 2778 3292 4086 4106 2778 Size: 514 794 20 4448 Fragments arranged by size: 4448 794 514 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DraIII CAC_nnn'GTG Cuts at: 2089 2173 2089 Size: 84 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2935 4783 809 Size: 507 1619 1848 1802 Fragments arranged by size: 1848 1802 1619 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 3223 4112 Size: 54 1425 50 275 18 243 1150 889 Cuts at: 4112 5741 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1150 889 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2274 2539 2571 3706 1353 Size: 921 265 32 1135 3423 Fragments arranged by size: 3423 1135 921 265 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4363 4737 4883 387 Size: 1064 138 435 2339 374 146 1280 Fragments arranged by size: 2339 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2581 2581 Size: 5776 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2424 3839 4436 5223 5491 1239 Size: 393 792 1415 597 787 268 1524 Fragments arranged by size: 1524 1415 792 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3044 3045 3044 Size: 1 5775 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 EcoRV GAT'ATC Cuts at: 2309 2713 2309 Size: 404 5372 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 4923 1678 Size: 3245 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2397 2429 2697 2729 4417 Size: 32 135 32 825 32 268 32 1688 Cuts at: 4417 4449 4491 4523 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1688 825 268 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3120 3366 669 Size: 988 281 1182 246 3079 Fragments arranged by size: 3079 1182 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2377 2474 4890 5074 1305 Size: 1072 97 2416 184 2007 Fragments arranged by size: 2416 2007 1072 184 97 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3954 Fragments arranged by size: 3954 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2262 2766 3734 4093 4252 518 Size: 648 1096 504 968 359 159 2042 Fragments arranged by size: 2042 1096 968 648 504 359 159 MspA1I CmG'CkG Cuts at: 1606 2851 3192 3869 5017 5262 1606 Size: 1245 341 677 1148 245 2120 Fragments arranged by size: 2120 1245 1148 677 341 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3060 513 Size: 552 939 1056 3229 Fragments arranged by size: 3229 1056 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 2227 2633 3134 4679 1132 Size: 97 998 406 501 1545 2229 Fragments arranged by size: 2229 1545 998 501 406 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 2173 2493 1108 Size: 1065 320 4391 Fragments arranged by size: 4391 1065 320 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PspOMI G'GGCC_C Cuts at: 3044 3044 Size: 5776 PstI C_TGCA'G Cuts at: 830 2019 2361 3128 830 Size: 1189 342 767 3478 Fragments arranged by size: 3478 1189 767 342 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2851 2851 Size: 5776 SacI G_AGCT'C Cuts at: 2583 2583 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SapI GCTCTTCn'nnn_ Cuts at: 2571 2571 Size: 5776 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 2888 4024 5736 2888 Size: 1136 1712 2928 Fragments arranged by size: 2928 1712 1136 SfcI C'TryA_G Cuts at: 826 1045 2015 2357 3124 4259 4940 5131 826 Size: 219 970 342 767 1135 681 191 1471 Fragments arranged by size: 1471 1135 970 767 681 342 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3051 3103 1872 Size: 1179 52 4545 Fragments arranged by size: 4545 1179 52 SmlI C'TyrA_G Cuts at: 820 1017 1943 2819 3880 4781 5043 820 Size: 197 926 876 1061 901 262 1553 Fragments arranged by size: 1553 1061 926 901 876 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 2526 2879 1517 Size: 1009 353 4414 Fragments arranged by size: 4414 1009 353 StyI C'CwwG_G Cuts at: 513 1065 2004 2487 2554 3060 3215 513 Size: 552 939 483 67 506 155 3074 Fragments arranged by size: 3074 939 552 506 483 155 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2650 2819 2888 3803 3820 3973 4158 Size: 1255 1070 169 69 915 17 153 185 Cuts at: 4158 4603 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1070 915 445 185 169 153 69 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2610 2658 2886 Size: 274 80 33 51 1443 633 48 228 Cuts at: 2886 4022 5734 96 Size: 1136 1712 138 Fragments arranged by size: 1712 1443 1136 633 274 228 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2277 2309 5736 5768 2277 Size: 32 3427 32 2285 Fragments arranged by size: 3427 2285 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2494 2494 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3049 3101 1870 Size: 1179 52 4545 Fragments arranged by size: 4545 1179 52 XmnI GAAnn'nnTTC Cuts at: 2235 3034 3905 2235 Size: 799 871 4106 Fragments arranged by size: 4106 871 799 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI