(Circular) (Six-base) MAPSORT of: pFN21AA1238.seq Check: 1106 from: 1 to: 5863 pFN21AA1238 5863 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3673 278 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3206 1937 Size: 1269 4594 Acc65I G'GTAC_C Cuts at: 3184 3184 Size: 5863 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2469 3926 5310 5578 1632 Size: 837 1457 1384 268 1917 Fragments arranged by size: 1917 1457 1384 837 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5666 AflIII A'CryG_T Cuts at: 1203 2617 4762 5739 1203 Size: 1414 2145 977 1327 Fragments arranged by size: 2145 1414 1327 977 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 2046 4592 2046 Size: 2546 3317 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5863 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2813 1184 Size: 1629 4234 AlwNI CAG_nnn'CTG Cuts at: 3606 5178 3606 Size: 1572 4291 ApaLI G'TGCA_C Cuts at: 3920 5076 3920 Size: 1156 4707 ApoI r'AATT_y Cuts at: 1493 2422 3172 3398 3550 5659 1493 Size: 929 750 226 152 2109 1697 Fragments arranged by size: 2109 1697 929 750 226 152 AseI AT'TA_AT Cuts at: 160 1032 4417 160 Size: 872 3385 1606 Fragments arranged by size: 3385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 2517 3188 1870 Size: 73 574 671 4545 Fragments arranged by size: 4545 671 574 73 BamHI G'GATC_C Cuts at: 3193 3560 3193 Size: 367 5496 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2648 3184 4639 618 Size: 325 217 627 861 536 1455 1842 Fragments arranged by size: 1842 1455 861 627 536 325 217 BanII G_rGCy'C Cuts at: 1391 3182 1391 Size: 1791 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2067 928 Size: 971 168 4724 Fragments arranged by size: 4724 971 168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2408 2442 4054 4088 1903 Size: 34 471 34 1612 34 3678 Fragments arranged by size: 3678 1612 471 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2389 3755 4965 2389 Size: 1366 1210 3287 Fragments arranged by size: 3287 1366 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4474 136 Size: 107 122 71 631 605 2802 1525 Fragments arranged by size: 2802 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5858 5858 Size: 5863 BlpI GC'TnA_GC Cuts at: 3280 3280 Size: 5863 Bme1580I G_kGCm'C Cuts at: 1790 3924 5080 1790 Size: 2134 1156 2573 Fragments arranged by size: 2573 2134 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5863 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3594 4552 442 Size: 416 53 83 399 24 2177 958 1753 Fragments arranged by size: 2177 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2750 3137 1056 Size: 1694 387 3782 Fragments arranged by size: 3782 1694 387 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3107 3107 Size: 5863 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2477 4523 1239 Size: 1238 2046 2579 Fragments arranged by size: 2579 2046 1238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2307 3988 4853 5151 2307 Size: 1681 865 298 3019 Fragments arranged by size: 3019 1681 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4526 882 Size: 617 3027 2219 Fragments arranged by size: 3027 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2618 3654 493 Size: 711 1414 1036 2702 Fragments arranged by size: 2702 1414 1036 711 BsaBI GATnn'nnATC Cuts at: 5857 5857 Size: 5863 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3670 4052 4687 275 Size: 53 83 186 936 2137 382 635 1451 Fragments arranged by size: 2137 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4296 4694 4968 5115 5526 4296 Size: 398 274 147 411 4633 Fragments arranged by size: 4633 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2625 2655 1178 Size: 30 165 30 1222 30 4386 Fragments arranged by size: 4386 1222 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 1186 Size: 940 4923 BseYI C'CCAG_C Cuts at: 3001 3010 5066 5562 3001 Size: 9 2056 496 3302 Fragments arranged by size: 3302 2056 496 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3313 4074 5102 1540 Size: 275 186 1312 761 1028 2301 Fragments arranged by size: 2301 1312 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2723 3924 4009 5080 2723 Size: 1201 85 1071 3506 Fragments arranged by size: 3506 1201 1071 85 BsmI GAATG_Cn' Cuts at: 2685 3374 3467 2685 Size: 689 93 5081 Fragments arranged by size: 5081 689 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2723 3182 3924 4009 5080 1391 Size: 399 933 459 742 85 1071 2174 Fragments arranged by size: 2174 1071 933 742 459 399 85 BspEI T'CCGG_A Cuts at: 5526 5526 Size: 5863 BspHI T'CATG_A Cuts at: 3752 5482 3752 Size: 1730 4133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2805 3218 844 Size: 1961 413 3489 Fragments arranged by size: 3489 1961 413 BsrBI CCG'CTC Cuts at: 1394 1823 3750 1394 Size: 429 1927 3507 Fragments arranged by size: 3507 1927 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4358 4532 65 Size: 1183 3110 174 1396 Fragments arranged by size: 3110 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2934 4507 4694 1700 Size: 110 1124 1573 187 2869 Fragments arranged by size: 2869 1573 1124 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5863 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5863 BssSI C'ACGA_G Cuts at: 3923 4935 3923 Size: 1012 4851 BstAPI GCAn_nnn'nTGC Cuts at: 794 2909 794 Size: 2115 3748 BstBI TT'CG_AA Cuts at: 4667 4667 Size: 5863 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5863 BstXI CCAn_nnnn'nTGG Cuts at: 2247 2247 Size: 5863 BstYI r'GATC_y Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858 Size: 1221 367 385 17 1452 86 12 346 Cuts at: 5858 1972 Size: 1977 Fragments arranged by size: 1977 1452 1221 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2377 2964 513 Size: 552 1312 587 3412 Fragments arranged by size: 3412 1312 587 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2842 487 Size: 683 56 1616 3508 Fragments arranged by size: 3508 1616 683 56 BtsI GCAGTG_nn' Cuts at: 2205 2973 3018 3379 4173 4193 2205 Size: 768 45 361 794 20 3875 Fragments arranged by size: 3875 794 768 361 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5828 DraI TTT'AAA Cuts at: 3167 3167 Size: 5863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4870 809 Size: 507 3554 1802 Fragments arranged by size: 3554 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2374 2413 2932 Size: 54 1425 50 275 18 544 39 519 Cuts at: 2932 3310 4199 5828 8 Size: 378 889 1629 43 Fragments arranged by size: 1629 1425 889 544 519 378 275 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 3310 1537 Size: 275 1498 4090 Fragments arranged by size: 4090 1498 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3793 1353 Size: 2440 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4450 4824 4970 387 Size: 1064 138 2861 374 146 1280 Fragments arranged by size: 2861 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2469 2477 3926 4523 5310 5578 1239 Size: 393 837 8 1449 597 787 268 1524 Fragments arranged by size: 1524 1449 837 787 597 393 268 8 EcoO109I rG'GnC_Cy Cuts at: 3005 3109 3005 Size: 104 5759 EcoRI G'AATT_C Cuts at: 3172 3550 3172 Size: 378 5485 FspI TGC'GCA Cuts at: 4369 4369 Size: 5863 HaeII r_GCGC'y Cuts at: 1678 2570 5010 1678 Size: 892 2440 2531 Fragments arranged by size: 2531 2440 892 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2032 2064 2187 2219 2287 Size: 32 135 32 460 32 123 32 68 Cuts at: 2287 2319 2387 2419 2801 2833 4504 4536 4578 Size: 32 68 32 382 32 1671 32 42 Cuts at: 4578 4610 1373 Size: 32 2626 Fragments arranged by size: 2626 1671 460 382 135 123 68 68 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3028 3058 3207 3453 669 Size: 988 281 1090 30 149 246 3079 Fragments arranged by size: 3079 1090 988 281 246 149 30 HindIII A'AGCT_T Cuts at: 748 748 Size: 5863 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KpnI G_GTAC'C Cuts at: 3188 3188 Size: 5863 MfeI C'AATT_G Cuts at: 3462 3462 Size: 5863 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4977 5161 1305 Size: 3672 184 2007 Fragments arranged by size: 3672 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2376 2415 10 Size: 54 1425 343 544 39 3458 Fragments arranged by size: 3458 1425 544 343 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 2627 3821 4180 4339 518 Size: 648 1461 1194 359 159 2042 Fragments arranged by size: 2042 1461 1194 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3130 3279 3956 5104 5349 1606 Size: 1524 149 677 1148 245 2120 Fragments arranged by size: 2120 1524 1148 677 245 149 NaeI GCC'GGC Cuts at: 2936 2936 Size: 5863 NcoI C'CATG_G Cuts at: 513 1065 2377 513 Size: 552 1312 3999 Fragments arranged by size: 3999 1312 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5863 NgoMIV G'CCGG_C Cuts at: 2934 2934 Size: 5863 NheI G'CTAG_C Cuts at: 1052 2746 3133 1052 Size: 1694 387 3782 Fragments arranged by size: 3782 1694 387 NotI GC'GGCC_GC Cuts at: 3310 3310 Size: 5863 NspI r_CATG'y Cuts at: 1132 1229 3221 4766 1132 Size: 97 1992 1545 2229 Fragments arranged by size: 2229 1992 1545 97 PasI CC'CwG_GG Cuts at: 1348 3104 1348 Size: 1756 4107 PciI A'CATG_T Cuts at: 4762 4762 Size: 5863 PflMI CCAn_nnn'nTGG Cuts at: 1108 2031 1108 Size: 923 4940 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3028 3060 3932 3964 5445 5477 3028 Size: 32 872 32 1481 32 3414 Fragments arranged by size: 3414 1481 872 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3005 3005 Size: 5863 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5863 PsiI TTA'TAA Cuts at: 3433 3433 Size: 5863 PstI C_TGCA'G Cuts at: 830 3215 830 Size: 2385 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 PvuII CAG'CTG Cuts at: 3130 3130 Size: 5863 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SanDI GG'GwC_CC Cuts at: 3005 3005 Size: 5863 SbfI CC_TGCA'GG Cuts at: 3215 3215 Size: 5863 ScaI AGT'ACT Cuts at: 4111 5823 4111 Size: 1712 4151 SfcI C'TryA_G Cuts at: 826 1045 3211 4346 5027 5218 826 Size: 219 2166 1135 681 191 1471 Fragments arranged by size: 2166 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5863 SmaI CCC'GGG Cuts at: 1872 3190 1872 Size: 1318 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2322 2517 3967 4868 5130 820 Size: 197 926 379 195 1450 901 262 1553 Fragments arranged by size: 1553 1450 926 901 379 262 197 195 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 2987 152 Size: 2835 3028 SphI G_CATG'C Cuts at: 1132 3221 1132 Size: 2089 3774 SspI AAT'ATT Cuts at: 5 52 3787 5 Size: 47 3735 2081 Fragments arranged by size: 3735 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5863 StyI C'CwwG_G Cuts at: 513 1065 2246 2377 3302 513 Size: 552 1181 131 925 3074 Fragments arranged by size: 3074 1181 925 552 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2239 3890 3907 4060 4245 4690 325 Size: 1255 659 1651 17 153 185 445 1498 Fragments arranged by size: 1651 1498 1255 659 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4109 5821 96 Size: 274 80 33 51 1443 2132 1712 138 Fragments arranged by size: 2132 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2625 2657 5823 5855 2625 Size: 32 3166 32 2633 Fragments arranged by size: 3166 2633 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5863 XbaI T'CTAG_A Cuts at: 2287 2881 3199 2287 Size: 594 318 4951 Fragments arranged by size: 4951 594 318 XhoI C'TCGA_G Cuts at: 1943 2517 1943 Size: 574 5289 XmaI C'CCGG_G Cuts at: 1870 3188 1870 Size: 1318 4545 XmnI GAAnn'nnTTC Cuts at: 3992 3992 Size: 5863 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3671 276 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI