(Circular) (Six-base) MAPSORT of: pFN21AA1237.seq Check: 8078 from: 1 to: 9151 pFN21AA1237 9151 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2643 5331 2643 Size: 2688 6463 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6961 278 Size: 53 83 186 936 5425 2468 Fragments arranged by size: 5425 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4418 6494 1937 Size: 2481 2076 4594 Fragments arranged by size: 4594 2481 2076 Acc65I G'GTAC_C Cuts at: 5469 6472 5469 Size: 1003 8148 AclI AA'CG_TT Cuts at: 1563 3589 5603 7278 7651 1563 Size: 2026 2014 1675 373 3063 Fragments arranged by size: 3063 2026 2014 1675 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3438 3459 4305 4868 5202 5238 7214 8598 Size: 1806 21 846 563 334 36 1976 1384 Cuts at: 8598 8866 1632 Size: 268 1917 Fragments arranged by size: 1976 1917 1806 1384 846 563 334 268 36 21 AflII C'TTAA_G Cuts at: 820 1017 4640 820 Size: 197 3623 5331 Fragments arranged by size: 5331 3623 197 AflIII A'CryG_T Cuts at: 1203 8050 9027 1203 Size: 6847 977 1327 Fragments arranged by size: 6847 1327 977 AgeI A'CCGG_T Cuts at: 7982 7982 Size: 9151 AhdI GACnn_n'nnGTC Cuts at: 3051 7880 3051 Size: 4829 4322 AleI CACnn'nnGTG Cuts at: 1166 6059 1166 Size: 4893 4258 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9151 AlwNI CAG_nnn'CTG Cuts at: 6894 8466 6894 Size: 1572 7579 ApaI G_GGCC'C Cuts at: 2232 2260 2305 2232 Size: 28 45 9078 Fragments arranged by size: 9078 45 28 ApaLI G'TGCA_C Cuts at: 2750 3885 7208 8364 2750 Size: 1135 3323 1156 3537 Fragments arranged by size: 3537 3323 1156 1135 ApoI r'AATT_y Cuts at: 1493 2842 3191 3218 3565 6460 6686 6838 8947 Size: 1349 349 27 347 2895 226 152 2109 Cuts at: 8947 1493 Size: 1697 Fragments arranged by size: 2895 2109 1697 1349 349 347 226 152 27 AseI AT'TA_AT Cuts at: 160 1032 4227 7705 160 Size: 872 3195 3478 1606 Fragments arranged by size: 3478 3195 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9151 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2214 3025 6476 1870 Size: 73 147 124 811 3451 4545 Fragments arranged by size: 4545 3451 811 147 124 73 AvrII C'CTAG_G Cuts at: 6097 6097 Size: 9151 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5049 5082 5049 Size: 33 9118 BamHI G'GATC_C Cuts at: 5441 6481 6848 5441 Size: 1040 367 7744 Fragments arranged by size: 7744 1040 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2140 2270 3670 3683 3818 Size: 325 217 627 353 130 1400 13 135 Cuts at: 3818 4880 5343 5469 6472 7927 618 Size: 1062 463 126 1003 1455 1842 Fragments arranged by size: 1842 1455 1400 1062 1003 627 463 353 325 217 135 130 126 13 BanII G_rGCy'C Cuts at: 1391 2232 2260 2305 2786 2969 4058 6087 6470 Size: 841 28 45 481 183 1089 2029 383 Cuts at: 6470 1391 Size: 4072 Fragments arranged by size: 4072 2029 1089 841 481 383 183 45 28 BbeI G_GCGC'C Cuts at: 2144 2274 2144 Size: 130 9021 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2621 3528 3609 4437 4856 5190 5282 Size: 971 722 907 81 828 419 334 92 Cuts at: 5282 928 Size: 4797 Fragments arranged by size: 4797 971 907 828 722 419 334 92 81 BbvCI CC'TCA_GC Cuts at: 5781 5781 Size: 9151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3016 3050 3129 3163 7342 7376 1903 Size: 34 1079 34 79 34 4179 34 3678 Fragments arranged by size: 4179 3678 1079 79 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4480 5915 7043 8253 4480 Size: 1435 1128 1210 5378 Fragments arranged by size: 5378 1435 1210 1128 BclI T'GATC_A Cuts at: 1548 1548 Size: 9151 BfrBI ATG'CAT Cuts at: 2995 4430 6250 2995 Size: 1435 1820 5896 Fragments arranged by size: 5896 1820 1435 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5356 6012 7762 Size: 107 122 71 631 605 3684 656 1750 Cuts at: 7762 136 Size: 1525 Fragments arranged by size: 3684 1750 1525 656 631 605 122 107 71 BglII A'GATC_T Cuts at: 2857 9146 2857 Size: 6289 2862 BlpI GC'TnA_GC Cuts at: 2132 4164 6568 2132 Size: 2032 2404 4715 Fragments arranged by size: 4715 2404 2032 Bme1580I G_kGCm'C Cuts at: 1790 2232 2260 2305 2754 3675 3889 5348 7212 Size: 442 28 45 449 921 214 1459 1864 Cuts at: 7212 8368 1790 Size: 1156 2573 Fragments arranged by size: 2573 1864 1459 1156 921 449 442 214 45 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9151 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3180 3460 6882 Size: 416 53 83 399 24 1763 280 3422 Cuts at: 6882 7840 442 Size: 958 1753 Fragments arranged by size: 3422 1763 1753 958 416 399 280 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3838 4120 6101 3838 Size: 282 1981 6888 Fragments arranged by size: 6888 1981 282 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2195 2460 2597 2945 3079 3513 3865 Size: 944 12 265 137 348 134 434 352 Cuts at: 3865 6263 7811 1239 Size: 2398 1548 2579 Fragments arranged by size: 2579 2398 1548 944 434 352 348 265 137 134 12 Bpu10I CC'TnA_GC Cuts at: 2262 5781 2262 Size: 3519 5632 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7276 8141 8439 7276 Size: 865 298 7988 Fragments arranged by size: 7988 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3865 6029 7814 882 Size: 617 2366 2164 1785 2219 Fragments arranged by size: 2366 2219 2164 1785 617 BsaAI yAC'GTr Cuts at: 493 1204 3581 3832 6942 493 Size: 711 2377 251 3110 2702 Fragments arranged by size: 3110 2702 2377 711 251 BsaBI GATnn'nnATC Cuts at: 9145 9145 Size: 9151 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2141 2271 6958 7340 Size: 53 83 186 936 608 130 4687 382 Cuts at: 7340 7975 275 Size: 635 1451 Fragments arranged by size: 4687 1451 936 635 608 382 186 130 83 53 BsaWI w'CCGG_w Cuts at: 7584 7982 8256 8403 8814 7584 Size: 398 274 147 411 7921 Fragments arranged by size: 7921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4271 4301 4729 4759 1178 Size: 30 165 30 2868 30 428 30 5570 Fragments arranged by size: 5570 2868 428 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2889 2959 4273 4276 4279 4282 4291 4294 Size: 1703 70 1314 3 3 3 9 3 Cuts at: 4294 4297 6266 1186 Size: 3 1969 4071 Fragments arranged by size: 4071 1969 1703 1314 70 9 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2246 3899 5122 8354 8850 2246 Size: 1653 1223 3232 496 2547 Fragments arranged by size: 3232 2547 1653 1223 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3357 3812 5863 1163 Size: 573 1621 455 2051 4451 Fragments arranged by size: 4451 2051 1621 573 455 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2299 6601 7362 8390 1540 Size: 275 186 298 4302 761 1028 2301 Fragments arranged by size: 4302 2301 1028 761 298 275 186 BsiHKAI G_wGCw'C Cuts at: 2754 2969 3764 3889 4058 4655 7212 7297 8368 Size: 215 795 125 169 597 2557 85 1071 Cuts at: 8368 2754 Size: 3537 Fragments arranged by size: 3537 2557 1071 795 597 215 169 125 85 BsmI GAATG_Cn' Cuts at: 3454 5309 6379 6662 6755 3454 Size: 1855 1070 283 93 5850 Fragments arranged by size: 5850 1855 1070 283 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2260 2305 2754 2786 2969 3675 Size: 399 442 28 45 449 32 183 706 Cuts at: 3675 3764 3889 4058 4655 5348 6087 6470 7212 Size: 89 125 169 597 693 739 383 742 Cuts at: 7212 7297 8368 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 739 706 693 597 449 442 399 383 183 169 125 89 85 45 32 28 BspEI T'CCGG_A Cuts at: 8814 8814 Size: 9151 BspHI T'CATG_A Cuts at: 4605 7040 8770 4605 Size: 2435 1730 4986 Fragments arranged by size: 4986 2435 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2643 5331 6002 6506 844 Size: 1799 2688 671 504 3489 Fragments arranged by size: 3489 2688 1799 671 504 BsrBI CCG'CTC Cuts at: 1394 1823 2041 2156 2526 5825 7038 1394 Size: 429 218 115 370 3299 1213 3507 Fragments arranged by size: 3507 3299 1213 429 370 218 115 BsrDI GCAATG_nn' Cuts at: 65 1248 7646 7820 65 Size: 1183 6398 174 1396 Fragments arranged by size: 6398 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2080 7795 7982 1700 Size: 110 270 5715 187 2869 Fragments arranged by size: 5715 2869 270 187 110 BsrGI T'GTAC_A Cuts at: 96 3484 96 Size: 3388 5763 BssHII G'CGCG_C Cuts at: 1925 2017 2127 1925 Size: 92 110 8949 Fragments arranged by size: 8949 110 92 BssSI C'ACGA_G Cuts at: 7211 8223 7211 Size: 1012 8139 BstAPI GCAn_nnn'nTGC Cuts at: 794 5835 6003 794 Size: 5041 168 3942 Fragments arranged by size: 5041 3942 168 BstBI TT'CG_AA Cuts at: 7955 7955 Size: 9151 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9151 BstXI CCAn_nnnn'nTGG Cuts at: 5677 5677 Size: 9151 BstYI r'GATC_y Cuts at: 1972 2857 5441 5801 5814 6481 6848 7233 7250 Size: 885 2584 360 13 667 367 385 17 Cuts at: 7250 8702 8788 8800 9146 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2584 1977 1452 885 667 385 367 360 346 86 17 13 12 Bsu36I CC'TnA_GG Cuts at: 2848 2848 Size: 9151 BtgI C'CryG_G Cuts at: 513 1065 2004 2224 4439 5175 5326 6072 513 Size: 552 939 220 2215 736 151 746 3592 Fragments arranged by size: 3592 2215 939 746 736 552 220 151 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2738 6088 487 Size: 683 56 1512 3350 3550 Fragments arranged by size: 3550 3350 1512 683 56 BtsI GCAGTG_nn' Cuts at: 3147 3369 3725 5335 5380 5687 6667 7461 7481 Size: 222 356 1610 45 307 980 794 20 Cuts at: 7481 3147 Size: 4817 Fragments arranged by size: 4817 1610 980 794 356 307 222 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5080 5115 529 Size: 35 4516 35 4565 Fragments arranged by size: 4565 4516 35 35 DraI TTT'AAA Cuts at: 5524 6455 5524 Size: 931 8220 DraIII CAC_nnn'GTG Cuts at: 3581 3632 3581 Size: 51 9100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8158 809 Size: 507 6842 1802 Fragments arranged by size: 6842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2030 2296 2342 Size: 54 1425 50 275 18 200 266 46 Cuts at: 2342 3795 3873 6598 7487 9116 8 Size: 1453 78 2725 889 1629 43 Fragments arranged by size: 2725 1629 1453 1425 889 275 266 200 78 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2296 6598 1537 Size: 275 484 4302 4090 Fragments arranged by size: 4302 4090 484 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2447 2536 2831 4090 4300 4315 5257 5840 Size: 1094 89 295 1259 210 15 942 583 Cuts at: 5840 6433 7081 1353 Size: 593 648 3423 Fragments arranged by size: 3423 1259 1094 942 648 593 583 295 210 89 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3631 5827 7738 8112 8258 387 Size: 1064 138 2042 2196 1911 374 146 1280 Fragments arranged by size: 2196 2042 1911 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2967 4056 2967 Size: 1089 8062 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2195 2460 2597 2945 3079 3438 Size: 393 551 12 265 137 348 134 359 Cuts at: 3438 3459 3513 3865 4305 4868 5202 5238 6263 Size: 21 54 352 440 563 334 36 1025 Cuts at: 6263 7214 7811 8598 8866 1239 Size: 951 597 787 268 1524 Fragments arranged by size: 1524 1025 951 787 597 563 551 440 393 359 352 348 334 268 265 137 134 54 36 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 2265 2265 Size: 9151 EcoO109I rG'GnC_Cy Cuts at: 2101 2256 2257 2288 2302 3059 4325 4395 2101 Size: 155 1 31 14 757 1266 70 6857 Fragments arranged by size: 6857 1266 757 155 70 31 14 1 EcoRI G'AATT_C Cuts at: 3191 3565 6460 6838 3191 Size: 374 2895 378 5504 Fragments arranged by size: 5504 2895 378 374 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3444 3444 Size: 9151 FspI TGC'GCA Cuts at: 7657 7657 Size: 9151 HaeII r_GCGC'y Cuts at: 1678 2144 2182 2224 2274 8298 1678 Size: 466 38 42 50 6024 2531 Fragments arranged by size: 6024 2531 466 50 42 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2844 2876 2900 2932 3037 Size: 32 135 32 1272 32 24 32 105 Cuts at: 3037 3069 3317 3349 3524 3556 4271 4303 5381 Size: 32 248 32 175 32 715 32 1078 Cuts at: 5381 5413 5433 5465 7792 7824 7866 7898 1373 Size: 32 20 32 2327 32 42 32 2626 Fragments arranged by size: 2626 2327 1272 1078 715 248 175 135 105 42 32 32 32 32 32 32 32 32 32 32 32 32 24 20 HincII GTy'rAC Cuts at: 669 1657 1938 3462 5266 5761 6495 6741 669 Size: 988 281 1524 1804 495 734 246 3079 Fragments arranged by size: 3079 1804 1524 988 734 495 281 246 HindIII A'AGCT_T Cuts at: 748 2939 3559 5096 748 Size: 2191 620 1537 4803 Fragments arranged by size: 4803 2191 1537 620 HpaI GTT'AAC Cuts at: 6741 6741 Size: 9151 KasI G'GCGC_C Cuts at: 2140 2270 2140 Size: 130 9021 KpnI G_GTAC'C Cuts at: 5473 6476 5473 Size: 1003 8148 MfeI C'AATT_G Cuts at: 6750 6750 Size: 9151 MluI A'CGCG_T Cuts at: 9027 9027 Size: 9151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4224 4850 5457 5694 8265 8449 1305 Size: 2919 626 607 237 2571 184 2007 Fragments arranged by size: 2919 2571 2007 626 607 237 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2344 10 Size: 54 1425 343 512 6817 Fragments arranged by size: 6817 1425 512 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3755 4229 4610 5420 6059 6223 6255 Size: 648 2589 474 381 810 639 164 32 Cuts at: 6255 7109 7468 7627 518 Size: 854 359 159 2042 Fragments arranged by size: 2589 2042 854 810 648 639 474 381 359 164 159 32 MspA1I CmG'CkG Cuts at: 1606 2062 2173 2226 2284 3794 4937 5271 6074 Size: 456 111 53 58 1510 1143 334 803 Cuts at: 6074 6567 7244 8392 8637 1606 Size: 493 677 1148 245 2120 Fragments arranged by size: 2120 1510 1148 1143 803 677 493 456 334 245 111 58 53 NaeI GCC'GGC Cuts at: 2082 2082 Size: 9151 NarI GG'CG_CC Cuts at: 2141 2271 2141 Size: 130 9021 NcoI C'CATG_G Cuts at: 513 1065 2004 4439 513 Size: 552 939 2435 5225 Fragments arranged by size: 5225 2435 939 552 NdeI CA'TA_TG Cuts at: 387 6190 387 Size: 5803 3348 NgoMIV G'CCGG_C Cuts at: 2080 2080 Size: 9151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9151 NotI GC'GGCC_GC Cuts at: 6598 6598 Size: 9151 NsiI A_TGCA'T Cuts at: 2997 4432 6252 2997 Size: 1435 1820 5896 Fragments arranged by size: 5896 1820 1435 NspI r_CATG'y Cuts at: 1132 1229 2999 5964 6509 8054 1132 Size: 97 1770 2965 545 1545 2229 Fragments arranged by size: 2965 2229 1770 1545 545 97 PasI CC'CwG_GG Cuts at: 1348 4551 1348 Size: 3203 5948 PciI A'CATG_T Cuts at: 8050 8050 Size: 9151 PflMI CCAn_nnn'nTGG Cuts at: 1108 3263 3694 4440 5797 1108 Size: 2155 431 746 1357 4462 Fragments arranged by size: 4462 2155 1357 746 431 PmeI GTTT'AAAC Cuts at: 6455 6455 Size: 9151 PmlI CAC'GTG Cuts at: 3581 3581 Size: 9151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7220 7252 8733 8765 7220 Size: 32 1481 32 7606 Fragments arranged by size: 7606 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2101 2288 3059 2101 Size: 187 771 8193 Fragments arranged by size: 8193 771 187 PshAI GACnn'nnGTC Cuts at: 1613 3229 1613 Size: 1616 7535 PsiI TTA'TAA Cuts at: 6721 6721 Size: 9151 PspOMI G'GGCC_C Cuts at: 2228 2256 2301 2228 Size: 28 45 9078 Fragments arranged by size: 9078 45 28 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3993 4025 3993 Size: 32 9119 PstI C_TGCA'G Cuts at: 830 2172 3142 3409 3793 4636 5342 6503 830 Size: 1342 970 267 384 843 706 1161 3478 Fragments arranged by size: 3478 1342 1161 970 843 706 384 267 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9151 PvuII CAG'CTG Cuts at: 2173 4937 5271 2173 Size: 2764 334 6053 Fragments arranged by size: 6053 2764 334 SacI G_AGCT'C Cuts at: 2969 4058 2969 Size: 1089 8062 SacII CC_GC'GG Cuts at: 2227 6075 2227 Size: 3848 5303 SalI G'TCGA_C Cuts at: 1936 6493 1936 Size: 4557 4594 SanDI GG'GwC_CC Cuts at: 2101 2288 2101 Size: 187 8964 SapI GCTCTTCn'nnn_ Cuts at: 2447 2536 2447 Size: 89 9062 SbfI CC_TGCA'GG Cuts at: 6503 6503 Size: 9151 ScaI AGT'ACT Cuts at: 7399 9111 7399 Size: 1712 7439 SfcI C'TryA_G Cuts at: 826 1045 2168 2251 2878 3138 3405 3789 4632 Size: 219 1123 83 627 260 267 384 843 Cuts at: 4632 4917 4932 5338 5646 6499 7634 8315 8506 Size: 285 15 406 308 853 1135 681 191 Cuts at: 8506 826 Size: 1471 Fragments arranged by size: 1471 1135 1123 853 843 681 627 406 384 308 285 267 260 219 191 83 15 SfoI GGC'GCC Cuts at: 2142 2272 2142 Size: 130 9021 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9151 SgrAI Cr'CCGG_yG Cuts at: 1700 2080 1700 Size: 380 8771 SmaI CCC'GGG Cuts at: 1872 2092 2216 6478 1872 Size: 220 124 4262 4545 Fragments arranged by size: 4545 4262 220 124 SmlI C'TyrA_G Cuts at: 820 1017 1943 4640 7255 8156 8418 820 Size: 197 926 2697 2615 901 262 1553 Fragments arranged by size: 2697 2615 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6942 493 Size: 6449 2702 SpeI A'CTAG_T Cuts at: 152 4972 152 Size: 4820 4331 SphI G_CATG'C Cuts at: 1132 6509 1132 Size: 5377 3774 SspI AAT'ATT Cuts at: 5 52 7075 5 Size: 47 7023 2081 Fragments arranged by size: 7023 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9151 StyI C'CwwG_G Cuts at: 513 1065 2004 3087 3597 3693 4439 6016 6097 Size: 552 939 1083 510 96 746 1577 81 Cuts at: 6097 6590 513 Size: 493 3074 Fragments arranged by size: 3074 1577 1083 939 746 552 510 493 96 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3364 3701 4234 7178 7195 7348 7533 Size: 1255 1784 337 533 2944 17 153 185 Cuts at: 7533 7978 325 Size: 445 1498 Fragments arranged by size: 2944 1784 1498 1255 533 445 337 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2930 3484 5115 Size: 274 80 33 51 1443 953 554 1631 Cuts at: 5115 6294 7397 9109 96 Size: 1179 1103 1712 138 Fragments arranged by size: 1712 1631 1443 1179 1103 953 554 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2624 2656 9111 9143 2624 Size: 32 6455 32 2632 Fragments arranged by size: 6455 2632 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3331 1316 Size: 2015 7136 XbaI T'CTAG_A Cuts at: 6487 6487 Size: 9151 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2862 2862 Size: 9151 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9151 XmaI C'CCGG_G Cuts at: 1870 2090 2214 6476 1870 Size: 220 124 4262 4545 Fragments arranged by size: 4545 4262 220 124 XmnI GAAnn'nnTTC Cuts at: 2628 7280 2628 Size: 4652 4499 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6959 276 Size: 53 83 186 936 5425 2468 Fragments arranged by size: 5425 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI PacI PfoI PspXI RsrII SexAI SfiI SrfI SwaI