Restriction map for
pFN21AA1237
(Linear) (Six-base) MAPSORT of: KIAA1237.seq Check: 5592 from: 1 to: 4446
KIAA1237.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 638 3326 4446
Size: 638 2688 1120
Fragments arranged by size:
2688 1120 638
AccI GT'mk_AC
Cuts at: 0 2413 4446
Size: 2413 2033
Acc65I G'GTAC_C
Cuts at: 0 3464 4446
Size: 3464 982
AclI AA'CG_TT
Cuts at: 0 1584 3598 4446
Size: 1584 2014 848
Fragments arranged by size:
2014 1584 848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1433 1454 2300 2863 3197 3233 4446
Size: 1433 21 846 563 334 36 1213
Fragments arranged by size:
1433 1213 846 563 334 36 21
AflII C'TTAA_G
Cuts at: 0 2635 4446
Size: 2635 1811
AhdI GACnn_n'nnGTC
Cuts at: 0 1046 4446
Size: 1046 3400
AleI CACnn'nnGTG
Cuts at: 0 4054 4446
Size: 4054 392
ApaI G_GGCC'C
Cuts at: 0 227 255 300 4446
Size: 227 28 45 4146
Fragments arranged by size:
4146 227 45 28
ApaLI G'TGCA_C
Cuts at: 0 745 1880 4446
Size: 745 1135 2566
Fragments arranged by size:
2566 1135 745
ApoI r'AATT_y
Cuts at: 0 837 1186 1213 1560 4446
Size: 837 349 27 347 2886
Fragments arranged by size:
2886 837 349 347 27
AseI AT'TA_AT
Cuts at: 0 2222 4446
Size: 2222 2224
AvaI C'yCGr_G
Cuts at: 0 85 209 1020 4446
Size: 85 124 811 3426
Fragments arranged by size:
3426 811 124 85
AvrII C'CTAG_G
Cuts at: 0 4092 4446
Size: 4092 354
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3044 3077 4446
Size: 3044 33 1369
Fragments arranged by size:
3044 1369 33
BamHI G'GATC_C
Cuts at: 0 3436 4446
Size: 3436 1010
BanI G'GyrC_C
Cuts at: 0 135 265 1665 1678 1813 2875 3338 3464
Size: 135 130 1400 13 135 1062 463 126
Cuts at: 3464 4446
Size: 982
Fragments arranged by size:
1400 1062 982 463 135 135 130 126
13
BanII G_rGCy'C
Cuts at: 0 227 255 300 781 964 2053 4082 4446
Size: 227 28 45 481 183 1089 2029 364
Fragments arranged by size:
2029 1089 481 364 227 183 45 28
BbeI G_GCGC'C
Cuts at: 0 139 269 4446
Size: 139 130 4177
Fragments arranged by size:
4177 139 130
BbsI GAAGACnn'nnnn_
Cuts at: 0 616 1523 1604 2432 2851 3185 3277 4446
Size: 616 907 81 828 419 334 92 1169
Fragments arranged by size:
1169 907 828 616 419 334 92 81
BbvCI CC'TCA_GC
Cuts at: 0 3776 4446
Size: 3776 670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1011 1045 1124 1158 4446
Size: 1011 34 79 34 3288
Fragments arranged by size:
3288 1011 79 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2475 3910 4446
Size: 2475 1435 536
Fragments arranged by size:
2475 1435 536
BfrBI ATG'CAT
Cuts at: 0 990 2425 4245 4446
Size: 990 1435 1820 201
Fragments arranged by size:
1820 1435 990 201
BglI GCCn_nnn'nGGC
Cuts at: 0 3351 4007 4446
Size: 3351 656 439
Fragments arranged by size:
3351 656 439
BglII A'GATC_T
Cuts at: 0 852 4446
Size: 852 3594
BlpI GC'TnA_GC
Cuts at: 0 127 2159 4446
Size: 127 2032 2287
Fragments arranged by size:
2287 2032 127
Bme1580I G_kGCm'C
Cuts at: 0 227 255 300 749 1670 1884 3343 4446
Size: 227 28 45 449 921 214 1459 1103
Fragments arranged by size:
1459 1103 921 449 227 214 45 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 1175 1455 4446
Size: 1175 280 2991
Fragments arranged by size:
2991 1175 280
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1833 2115 4096 4446
Size: 1833 282 1981 350
Fragments arranged by size:
1981 1833 350 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 190 455 592 940 1074 1508 1860
Size: 178 12 265 137 348 134 434 352
Cuts at: 1860 4258 4446
Size: 2398 188
Fragments arranged by size:
2398 434 352 348 265 188 178 137
134 12
Bpu10I CC'TnA_GC
Cuts at: 0 257 3776 4446
Size: 257 3519 670
Fragments arranged by size:
3519 670 257
BsaI GGTCTCn'nnnn_
Cuts at: 0 1860 4024 4446
Size: 1860 2164 422
Fragments arranged by size:
2164 1860 422
BsaAI yAC'GTr
Cuts at: 0 1576 1827 4446
Size: 1576 251 2619
Fragments arranged by size:
2619 1576 251
BsaHI Gr'CG_yC
Cuts at: 0 136 266 4446
Size: 136 130 4180
Fragments arranged by size:
4180 136 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2266 2296 2724 2754 4446
Size: 2266 30 428 30 1692
Fragments arranged by size:
2266 1692 428 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 884 954 2268 2271 2274 2277 2286 2289
Size: 884 70 1314 3 3 3 9 3
Cuts at: 2289 2292 4261 4446
Size: 3 1969 185
Fragments arranged by size:
1969 1314 884 185 70 9 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 241 1894 3117 4446
Size: 241 1653 1223 1329
Fragments arranged by size:
1653 1329 1223 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1352 1807 3858 4446
Size: 1352 455 2051 588
Fragments arranged by size:
2051 1352 588 455
BsiEI CG_ry'CG
Cuts at: 0 294 4446
Size: 294 4152
BsiHKAI G_wGCw'C
Cuts at: 0 749 964 1759 1884 2053 2650 4446
Size: 749 215 795 125 169 597 1796
Fragments arranged by size:
1796 795 749 597 215 169 125
BsmI GAATG_Cn'
Cuts at: 0 1449 3304 4374 4446
Size: 1449 1855 1070 72
Fragments arranged by size:
1855 1449 1070 72
Bsp1286I G_dGCh'C
Cuts at: 0 227 255 300 749 781 964 1670 1759
Size: 227 28 45 449 32 183 706 89
Cuts at: 1759 1884 2053 2650 3343 4082 4446
Size: 125 169 597 693 739 364
Fragments arranged by size:
739 706 693 597 449 364 227 183
169 125 89 45 32 28
BspHI T'CATG_A
Cuts at: 0 2600 4446
Size: 2600 1846
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 638 3326 3997 4446
Size: 638 2688 671 449
Fragments arranged by size:
2688 671 638 449
BsrBI CCG'CTC
Cuts at: 0 36 151 521 3820 4446
Size: 36 115 370 3299 626
Fragments arranged by size:
3299 626 370 115 36
BsrFI r'CCGG_y
Cuts at: 0 75 4446
Size: 75 4371
BsrGI T'GTAC_A
Cuts at: 0 1479 4446
Size: 1479 2967
BssHII G'CGCG_C
Cuts at: 0 12 122 4446
Size: 12 110 4324
Fragments arranged by size:
4324 110 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3830 3998 4446
Size: 3830 168 448
Fragments arranged by size:
3830 448 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3672 4446
Size: 3672 774
BstYI r'GATC_y
Cuts at: 0 852 3436 3796 3809 4446
Size: 852 2584 360 13 637
Fragments arranged by size:
2584 852 637 360 13
Bsu36I CC'TnA_GG
Cuts at: 0 843 4446
Size: 843 3603
BtgI C'CryG_G
Cuts at: 0 219 2434 3170 3321 4067 4446
Size: 219 2215 736 151 746 379
Fragments arranged by size:
2215 746 736 379 219 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 733 4083 4446
Size: 733 3350 363
Fragments arranged by size:
3350 733 363
BtsI GCAGTG_nn'
Cuts at: 0 1142 1364 1720 3330 3375 3682 4446
Size: 1142 222 356 1610 45 307 764
Fragments arranged by size:
1610 1142 764 356 307 222 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3075 3110 4446
Size: 3075 35 1336
Fragments arranged by size:
3075 1336 35
DraI TTT'AAA
Cuts at: 0 3519 4446
Size: 3519 927
DraIII CAC_nnn'GTG
Cuts at: 0 1576 1627 4446
Size: 1576 51 2819
Fragments arranged by size:
2819 1576 51
EaeI y'GGCC_r
Cuts at: 0 25 291 337 1790 1868 4446
Size: 25 266 46 1453 78 2578
Fragments arranged by size:
2578 1453 266 78 46 25
EagI C'GGCC_G
Cuts at: 0 291 4446
Size: 291 4155
EarI CTCTTCn'nnn_
Cuts at: 0 442 531 826 2085 2295 2310 3252 3835
Size: 442 89 295 1259 210 15 942 583
Cuts at: 3835 4428 4446
Size: 593 18
Fragments arranged by size:
1259 942 593 583 442 295 210 89
18 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1626 3822 4446
Size: 1626 2196 624
Fragments arranged by size:
2196 1626 624
EcoICRI GAG'CTC
Cuts at: 0 962 2051 4446
Size: 962 1089 2395
Fragments arranged by size:
2395 1089 962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 190 455 592 940 1074 1433 1454
Size: 178 12 265 137 348 134 359 21
Cuts at: 1454 1508 1860 2300 2863 3197 3233 4258 4446
Size: 54 352 440 563 334 36 1025 188
Fragments arranged by size:
1025 563 440 359 352 348 334 265
188 178 137 134 54 36 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 260 4446
Size: 260 4186
EcoO109I rG'GnC_Cy
Cuts at: 0 96 251 252 283 297 1054 2320 2390
Size: 96 155 1 31 14 757 1266 70
Cuts at: 2390 4446
Size: 2056
Fragments arranged by size:
2056 1266 757 155 96 70 31 14
1
EcoRI G'AATT_C
Cuts at: 0 1186 1560 4446
Size: 1186 374 2886
Fragments arranged by size:
2886 1186 374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1439 4446
Size: 1439 3007
HaeII r_GCGC'y
Cuts at: 0 139 177 219 269 4446
Size: 139 38 42 50 4177
Fragments arranged by size:
4177 139 50 42 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 839 871 895 927 1032 1064 1312 1344
Size: 839 32 24 32 105 32 248 32
Cuts at: 1344 1519 1551 2266 2298 3376 3408 3428 3460
Size: 175 32 715 32 1078 32 20 32
Cuts at: 3460 4446
Size: 986
Fragments arranged by size:
1078 986 839 715 248 175 105 32
32 32 32 32 32 32 32 24
20
HincII GTy'rAC
Cuts at: 0 1457 3261 3756 4446
Size: 1457 1804 495 690
Fragments arranged by size:
1804 1457 690 495
HindIII A'AGCT_T
Cuts at: 0 934 1554 3091 4446
Size: 934 620 1537 1355
Fragments arranged by size:
1537 1355 934 620
KasI G'GCGC_C
Cuts at: 0 135 265 4446
Size: 135 130 4181
Fragments arranged by size:
4181 135 130
KpnI G_GTAC'C
Cuts at: 0 3468 4446
Size: 3468 978
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2219 2845 3452 3689 4446
Size: 2219 626 607 237 757
Fragments arranged by size:
2219 757 626 607 237
MscI TGG'CCA
Cuts at: 0 339 4446
Size: 339 4107
MslI CAynn'nnrTG
Cuts at: 0 1750 2224 2605 3415 4054 4218 4250 4446
Size: 1750 474 381 810 639 164 32 196
Fragments arranged by size:
1750 810 639 474 381 196 164 32
MspA1I CmG'CkG
Cuts at: 0 57 168 221 279 1789 2932 3266 4069
Size: 57 111 53 58 1510 1143 334 803
Cuts at: 4069 4446
Size: 377
Fragments arranged by size:
1510 1143 803 377 334 111 58 57
53
NaeI GCC'GGC
Cuts at: 0 77 4446
Size: 77 4369
NarI GG'CG_CC
Cuts at: 0 136 266 4446
Size: 136 130 4180
Fragments arranged by size:
4180 136 130
NcoI C'CATG_G
Cuts at: 0 2434 4446
Size: 2434 2012
NdeI CA'TA_TG
Cuts at: 0 4185 4446
Size: 4185 261
NgoMIV G'CCGG_C
Cuts at: 0 75 4446
Size: 75 4371
NsiI A_TGCA'T
Cuts at: 0 992 2427 4247 4446
Size: 992 1435 1820 199
Fragments arranged by size:
1820 1435 992 199
NspI r_CATG'y
Cuts at: 0 994 3959 4446
Size: 994 2965 487
Fragments arranged by size:
2965 994 487
PasI CC'CwG_GG
Cuts at: 0 2546 4446
Size: 2546 1900
PflMI CCAn_nnn'nTGG
Cuts at: 0 1258 1689 2435 3792 4446
Size: 1258 431 746 1357 654
Fragments arranged by size:
1357 1258 746 654 431
PmlI CAC'GTG
Cuts at: 0 1576 4446
Size: 1576 2870
PpuMI rG'GwC_Cy
Cuts at: 0 96 283 1054 4446
Size: 96 187 771 3392
Fragments arranged by size:
3392 771 187 96
PshAI GACnn'nnGTC
Cuts at: 0 1224 4446
Size: 1224 3222
PspOMI G'GGCC_C
Cuts at: 0 223 251 296 4446
Size: 223 28 45 4150
Fragments arranged by size:
4150 223 45 28
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1988 2020 4446
Size: 1988 32 2426
Fragments arranged by size:
2426 1988 32
PstI C_TGCA'G
Cuts at: 0 167 1137 1404 1788 2631 3337 4446
Size: 167 970 267 384 843 706 1109
Fragments arranged by size:
1109 970 843 706 384 267 167
PvuII CAG'CTG
Cuts at: 0 168 2932 3266 4446
Size: 168 2764 334 1180
Fragments arranged by size:
2764 1180 334 168
SacI G_AGCT'C
Cuts at: 0 964 2053 4446
Size: 964 1089 2393
Fragments arranged by size:
2393 1089 964
SacII CC_GC'GG
Cuts at: 0 222 4070 4446
Size: 222 3848 376
Fragments arranged by size:
3848 376 222
SanDI GG'GwC_CC
Cuts at: 0 96 283 4446
Size: 96 187 4163
Fragments arranged by size:
4163 187 96
SapI GCTCTTCn'nnn_
Cuts at: 0 442 531 4446
Size: 442 89 3915
Fragments arranged by size:
3915 442 89
SfcI C'TryA_G
Cuts at: 0 163 246 873 1133 1400 1784 2627 2912
Size: 163 83 627 260 267 384 843 285
Cuts at: 2912 2927 3333 3641 4446
Size: 15 406 308 805
Fragments arranged by size:
843 805 627 406 384 308 285 267
260 163 83 15
SfoI GGC'GCC
Cuts at: 0 137 267 4446
Size: 137 130 4179
Fragments arranged by size:
4179 137 130
SgrAI Cr'CCGG_yG
Cuts at: 0 75 4446
Size: 75 4371
SmaI CCC'GGG
Cuts at: 0 87 211 4446
Size: 87 124 4235
Fragments arranged by size:
4235 124 87
SmlI C'TyrA_G
Cuts at: 0 2635 4446
Size: 2635 1811
SpeI A'CTAG_T
Cuts at: 0 2967 4446
Size: 2967 1479
StyI C'CwwG_G
Cuts at: 0 1082 1592 1688 2434 4011 4092 4446
Size: 1082 510 96 746 1577 81 354
Fragments arranged by size:
1577 1082 746 510 354 96 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1359 1696 2229 4446
Size: 1359 337 533 2217
Fragments arranged by size:
2217 1359 533 337
TatI w'GTAC_w
Cuts at: 0 925 1479 3110 4289 4446
Size: 925 554 1631 1179 157
Fragments arranged by size:
1631 1179 925 554 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 619 651 4446
Size: 619 32 3795
Fragments arranged by size:
3795 619 32
Tth111I GACn'n_nGTC
Cuts at: 0 1326 4446
Size: 1326 3120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 857 4446
Size: 857 3589
XmaI C'CCGG_G
Cuts at: 0 85 209 4446
Size: 85 124 4237
Fragments arranged by size:
4237 124 85
XmnI GAAnn'nnTTC
Cuts at: 0 623 4446
Size: 623 3823
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AhdI AleI
ApaI ApaLI ApoI AseI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI
BssHII BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PflMI
PmlI PpuMI PshAI PspOMI PsrI PstI PvuII SacI
SacII SanDI SapI SfcI SfoI SgrAI SmaI SmlI
SpeI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflIII AgeI AlfI AloI AlwNI AscI
AsiSI BclI BmgBI BmtI BpuEI BsaBI BsaWI BsiWI
BsmBI BspEI BsrDI BssSI BstBI BstEII BstZ17I ClaI
DrdI EcoRV FseI FspI FspAI HpaI MfeI MluI
NheI NotI NruI PacI PciI PfoI PmeI PpiI
PsiI PspXI PvuI RsrII SalI SbfI ScaI SexAI
SfiI SgfI SnaBI SphI SrfI SspI StuI SwaI
XbaI XhoI ZraI