(Circular) (Six-base) MAPSORT of: pFN21AA1191.seq Check: 1444 from: 1 to: 5620 pFN21AA1191 5620 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3430 278 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2233 2963 1937 Size: 296 730 4594 Fragments arranged by size: 4594 730 296 Acc65I G'GTAC_C Cuts at: 2941 2941 Size: 5620 AclI AA'CG_TT Cuts at: 1563 3747 4120 1563 Size: 2184 373 3063 Fragments arranged by size: 3063 2184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2681 2817 3683 5067 5335 1632 Size: 1049 136 866 1384 268 1917 Fragments arranged by size: 1917 1384 1049 866 268 136 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5423 AflIII A'CryG_T Cuts at: 1203 4519 5496 1203 Size: 3316 977 1327 Fragments arranged by size: 3316 1327 977 AgeI A'CCGG_T Cuts at: 4451 4451 Size: 5620 AhdI GACnn_n'nnGTC Cuts at: 4349 4349 Size: 5620 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5620 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5620 AlwNI CAG_nnn'CTG Cuts at: 2574 3363 4935 2574 Size: 789 1572 3259 Fragments arranged by size: 3259 1572 789 ApaI G_GGCC'C Cuts at: 2872 2872 Size: 5620 ApaLI G'TGCA_C Cuts at: 3677 4833 3677 Size: 1156 4464 ApoI r'AATT_y Cuts at: 1493 2323 2929 3155 3307 5416 1493 Size: 830 606 226 152 2109 1697 Fragments arranged by size: 2109 1697 830 606 226 152 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2114 2945 1870 Size: 73 171 831 4545 Fragments arranged by size: 4545 831 171 73 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2941 4396 618 Size: 325 217 627 1154 1455 1842 Fragments arranged by size: 1842 1455 1154 627 325 217 BanII G_rGCy'C Cuts at: 1391 2746 2872 2939 1391 Size: 1355 126 67 4072 Fragments arranged by size: 4072 1355 126 67 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4649 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2105 2139 3811 3845 1903 Size: 34 168 34 1672 34 3678 Fragments arranged by size: 3678 1672 168 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3512 4722 3512 Size: 1210 4410 BclI T'GATC_A Cuts at: 1548 2331 1548 Size: 783 4837 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2729 4231 136 Size: 107 122 71 631 605 1057 1502 1525 Fragments arranged by size: 1525 1502 1057 631 605 122 107 71 BglII A'GATC_T Cuts at: 5615 5615 Size: 5620 BlpI GC'TnA_GC Cuts at: 3037 3037 Size: 5620 Bme1580I G_kGCm'C Cuts at: 1790 2779 2872 3681 4837 1790 Size: 989 93 809 1156 2573 Fragments arranged by size: 2573 1156 989 809 93 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5620 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2282 2515 2831 Size: 416 53 83 399 24 865 233 316 Cuts at: 2831 3351 4309 442 Size: 520 958 1753 Fragments arranged by size: 1753 958 865 520 416 399 316 233 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4280 1239 Size: 3041 2579 Bpu10I CC'TnA_GC Cuts at: 2556 2556 Size: 5620 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2057 3745 4610 4908 2057 Size: 1688 865 298 2769 Fragments arranged by size: 2769 1688 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2423 4283 882 Size: 617 924 1860 2219 Fragments arranged by size: 2219 1860 924 617 BsaAI yAC'GTr Cuts at: 493 1204 2774 3411 493 Size: 711 1570 637 2702 Fragments arranged by size: 2702 1570 711 637 BsaBI GATnn'nnATC Cuts at: 2831 5614 2831 Size: 2783 2837 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3427 3809 4444 275 Size: 53 83 186 936 1894 382 635 1451 Fragments arranged by size: 1894 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4053 4451 4725 4872 5283 4053 Size: 398 274 147 411 4390 Fragments arranged by size: 4390 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2125 2155 2420 2450 1178 Size: 30 165 30 722 30 265 30 4348 Fragments arranged by size: 4348 722 265 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2127 2130 2441 2754 1186 Size: 941 3 311 313 4052 Fragments arranged by size: 4052 941 313 311 3 BseYI C'CCAG_C Cuts at: 4823 5319 4823 Size: 496 5124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5047 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3070 3831 4859 1540 Size: 275 186 1069 761 1028 2301 Fragments arranged by size: 2301 1069 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2904 3681 3766 4837 2904 Size: 777 85 1071 3687 Fragments arranged by size: 3687 1071 777 85 BsmI GAATG_Cn' Cuts at: 2355 3131 3224 2355 Size: 776 93 4751 Fragments arranged by size: 4751 776 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2746 2779 2872 2904 2939 3681 3766 Size: 399 956 33 93 32 35 742 85 Cuts at: 3766 4837 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 956 742 399 93 85 35 33 32 BspEI T'CCGG_A Cuts at: 5283 5283 Size: 5620 BspHI T'CATG_A Cuts at: 2319 3509 5239 2319 Size: 1190 1730 2700 Fragments arranged by size: 2700 1730 1190 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2975 844 Size: 2131 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3507 1394 Size: 429 1684 3507 Fragments arranged by size: 3507 1684 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4115 4289 65 Size: 1183 2867 174 1396 Fragments arranged by size: 2867 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4264 4451 1700 Size: 110 2454 187 2869 Fragments arranged by size: 2869 2454 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5620 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 3680 4692 3680 Size: 1012 4608 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5620 BstBI TT'CG_AA Cuts at: 4424 4424 Size: 5620 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5620 BstXI CCAn_nnnn'nTGG Cuts at: 2791 2791 Size: 5620 BstYI r'GATC_y Cuts at: 1972 2690 2800 2950 3317 3702 3719 5171 5257 Size: 718 110 150 367 385 17 1452 86 Cuts at: 5257 5269 5615 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 718 385 367 346 150 110 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2625 2864 513 Size: 552 939 621 239 3269 Fragments arranged by size: 3269 939 621 552 239 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4881 Fragments arranged by size: 4881 683 56 BtsI GCAGTG_nn' Cuts at: 3136 3930 3950 3136 Size: 794 20 4806 Fragments arranged by size: 4806 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5620 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5585 DraI TTT'AAA Cuts at: 2924 2924 Size: 5620 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4627 809 Size: 507 3311 1802 Fragments arranged by size: 3311 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2448 3067 3956 Size: 54 1425 50 275 18 618 619 889 Cuts at: 3956 5585 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 619 618 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3067 1537 Size: 275 1255 4090 Fragments arranged by size: 4090 1255 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2493 2554 3550 1353 Size: 1140 61 996 3423 Fragments arranged by size: 3423 1140 996 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4207 4581 4727 387 Size: 1064 138 2618 374 146 1280 Fragments arranged by size: 2618 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2681 2817 3683 4280 5067 5335 1239 Size: 393 1049 136 866 597 787 268 1524 Fragments arranged by size: 1524 1049 866 787 597 393 268 136 EcoNI CCTnn'n_nnAGG Cuts at: 2207 2207 Size: 5620 EcoO109I rG'GnC_Cy Cuts at: 2119 2284 2413 2119 Size: 165 129 5326 Fragments arranged by size: 5326 165 129 EcoRI G'AATT_C Cuts at: 2323 2929 3307 2323 Size: 606 378 4636 Fragments arranged by size: 4636 606 378 FspI TGC'GCA Cuts at: 4126 4126 Size: 5620 HaeII r_GCGC'y Cuts at: 1678 4767 1678 Size: 3089 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2125 2157 4261 4293 4335 Size: 32 135 32 553 32 2104 32 42 Cuts at: 4335 4367 1373 Size: 32 2626 Fragments arranged by size: 2626 2104 553 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2964 3210 669 Size: 988 281 1026 246 3079 Fragments arranged by size: 3079 1026 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5620 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2945 2945 Size: 5620 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4734 4918 1305 Size: 3429 184 2007 Fragments arranged by size: 3429 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3798 Fragments arranged by size: 3798 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2837 3578 3937 4096 518 Size: 648 1671 741 359 159 2042 Fragments arranged by size: 2042 1671 741 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3036 3713 4861 5106 1606 Size: 1430 677 1148 245 2120 Fragments arranged by size: 2120 1430 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2625 513 Size: 552 939 621 3508 Fragments arranged by size: 3508 939 621 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5620 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NspI r_CATG'y Cuts at: 1132 1229 2978 4523 1132 Size: 97 1749 1545 2229 Fragments arranged by size: 2229 1749 1545 97 PasI CC'CwG_GG Cuts at: 1348 2568 1348 Size: 1220 4400 PciI A'CATG_T Cuts at: 4519 4519 Size: 5620 PflMI CCAn_nnn'nTGG Cuts at: 1108 2574 1108 Size: 1466 4154 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3689 3721 5202 5234 3689 Size: 32 1481 32 4075 Fragments arranged by size: 4075 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2284 2119 Size: 165 5455 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5620 PsiI TTA'TAA Cuts at: 3190 3190 Size: 5620 PspOMI G'GGCC_C Cuts at: 2868 2868 Size: 5620 PspXI vC'TCGA_Gb Cuts at: 2114 2114 Size: 5620 PstI C_TGCA'G Cuts at: 830 2972 830 Size: 2142 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 SalI G'TCGA_C Cuts at: 1936 2962 1936 Size: 1026 4594 SbfI CC_TGCA'GG Cuts at: 2972 2972 Size: 5620 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SfcI C'TryA_G Cuts at: 826 1045 2968 4103 4784 4975 826 Size: 219 1923 1135 681 191 1471 Fragments arranged by size: 1923 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2947 1872 Size: 1075 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2036 2114 3724 4625 4887 820 Size: 197 926 93 78 1610 901 262 1553 Fragments arranged by size: 1610 1553 926 901 262 197 93 78 SnaBI TAC'GTA Cuts at: 493 3411 493 Size: 2918 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2978 1132 Size: 1846 3774 SspI AAT'ATT Cuts at: 5 52 3544 5 Size: 47 3492 2081 Fragments arranged by size: 3492 2081 47 StuI AGG'CCT Cuts at: 1517 2732 1517 Size: 1215 4405 StyI C'CwwG_G Cuts at: 513 1065 2004 2165 2208 2625 3059 513 Size: 552 939 161 43 417 434 3074 Fragments arranged by size: 3074 939 552 434 417 161 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2701 2741 2923 3647 3664 3817 4002 Size: 1255 1121 40 182 724 17 153 185 Cuts at: 4002 4447 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1121 724 445 185 182 153 40 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3866 5578 96 Size: 274 80 33 51 1443 1889 1712 138 Fragments arranged by size: 1889 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5580 5612 5580 Size: 32 5588 Tth111I GACn'n_nGTC Cuts at: 1316 2254 2282 1316 Size: 938 28 4654 Fragments arranged by size: 4654 938 28 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2272 2272 Size: 5620 XhoI C'TCGA_G Cuts at: 1943 2114 1943 Size: 171 5449 XmaI C'CCGG_G Cuts at: 1870 2945 1870 Size: 1075 4545 XmnI GAAnn'nnTTC Cuts at: 2355 3749 2355 Size: 1394 4226 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3428 276 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI