Restriction map for
pFN21AA1161
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# NO CUT ENZYMES ( 101 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI AocI ApaI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
BspDI BspEI BspHI BsrDI BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraII DraIII
Eam1105I EarI Eco81I EcoO109I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI Ksp632I MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SapI SbfI
ScaI SexAI SfiI SfuI SmaI
SnaBI SpeI Sse8387I SspI SwaI
TfiI Tru9I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 1003 AAATTC [GA]^AATT[CT]
Alw44I 1069 GTGCAC G^TGCAC
AlwNI 1347 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1069 GTGCAC G^TGCAC
ApoI 1003 AAATTC [GA]^AATT[CT]
AscI 872 GGCGCGCC GG^CGCGCC
AspI 358 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 226 GTCTTC GAAGAC(2/6)
BfaI 1195 CTAG C^TAG
BlpI 732 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 732 GCTGAGC GC^T[ACGT]AGC
BpuAI 226 GTCTTC GAAGAC(2/6)
BsaI 839 GAGACC GGTCTC(1/5)
BsmBI 135 CGTCTC CGTCTC(1/5)
BsrBI 243 CCGCTC CCGCTC(-3/-3)
BstYI 914 AGATCC [GA]^GATC[CT]
CelII 732 GCTGAGC GC^T[ACGT]AGC
DrdI 1342 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 141 CCTACGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 353 CCAAGG C^C[AT][AT]GG
MaeI 1195 CTAG C^TAG
MflI 914 AGATCC [GA]^GATC[CT]
NotI 598 GCGGCCGC GC^GGCCGC
NruI 1458 TCGCGA TCG^CGA
PaeR7I 571 CTCGAG C^TCGAG
PflFI 358 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1346 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 270 CTGCAG CTGCA^G
SacI 1612 GAGCTC GAGCT^C
SalI 2002 GTCGAC G^TCGAC
SgrAI 1976 CGCCGGTG C[GA]^CCGG[CT]G
SphI 1523 GCATGC GCATG^C
StuI 830 AGGCCT AGG^CCT
StyI 353 CCAAGG C^C[AT][AT]GG
Tsp509I 1004 AATT ^AATT
Tth111I 358 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1346 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 447 CCACTGGTATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 571 CTCGAG C^TCGAG
XhoII 914 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 760 GACGTC GACGT^C
1918 GACGTC
AccI 538 GTCTAC GT^[AC][GT]AC
2002 GTCGAC
AflIII 959 ACATGT A^C[GA][CT]GT
1022 ACATGT
AlwI 915 GATCC GGATC(4/5)
1455 GGATC
Aor51HI 575 AGCGCT AGC^GCT
656 AGCGCT
BalI 501 TGGCCA TGG^CCA
1700 TGGCCA
BbeI 283 GGCGCC GGCGC^C
460 GGCGCC
BpmI 1353 CTGGAG CTGGAG(16/14)
1894 CTGGAG
BseRI 543 CTCCTC GAGGAG(10/8)
388 GAGGAG
BsgI 325 CTGCAC GTGCAG(16/14)
922 CTGCAC
Bsp1407I 2 TGTACA T^GTACA
962 TGTACA
BspLU11I 959 ACATGT A^CATGT
1022 ACATGT
BsrGI 2 TGTACA T^GTACA
962 TGTACA
Eco47III 575 AGCGCT AGC^GCT
656 AGCGCT
HincII 1081 GTCAAC GT[CT]^[GA]AC
2002 GTCGAC
HindII 1081 GTCAAC GT[CT]^[GA]AC
2002 GTCGAC
KasI 283 GGCGCC G^GCGCC
460 GGCGCC
MaeII 761 ACGT A^CGT
1919 ACGT
MluNI 501 TGGCCA TGG^CCA
1700 TGGCCA
MscI 501 TGGCCA TGG^CCA
1700 TGGCCA
NarI 283 GGCGCC GG^CGCC
460 GGCGCC
SfaNI 566 GCATC GCATC(5/9)
1181 GCATC
SfcI 129 CTACAG C^T[GA][CT]AG
270 CTGCAG
SfoI 283 GGCGCC GGC^GCC
460 GGCGCC
SmlI 571 CTCGAG C^T[CT][GA]AG
1756 CTTGAG
SspBI 2 TGTACA T^GTACA
962 TGTACA
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BsaHI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssHII BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EagI EclXI
Eco52I EcoO65I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI RsaI SacII Sau3AI Sau96I
ScrFI TaqI TseI Tsp45I TspRI
TthHB8I