(Circular) (Six-base) MAPSORT of: pFN21AA1161.seq Check: 9394 from: 1 to: 6745 pFN21AA1161 6745 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2044 2983 3252 2044 Size: 939 269 5537 Fragments arranged by size: 5537 939 269 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2769 3927 4555 278 Size: 53 83 186 936 1233 1158 628 2468 Fragments arranged by size: 2468 1233 1158 936 628 186 83 53 AccI GT'mk_AC Cuts at: 1937 2544 4008 4088 1937 Size: 607 1464 80 4594 Fragments arranged by size: 4594 1464 607 80 Acc65I G'GTAC_C Cuts at: 4066 4066 Size: 6745 AclI AA'CG_TT Cuts at: 1563 4872 5245 1563 Size: 3309 373 3063 Fragments arranged by size: 3309 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2084 2660 3307 4808 6192 6460 1632 Size: 452 576 647 1501 1384 268 1917 Fragments arranged by size: 1917 1501 1384 647 576 452 268 AfeI AGC'GCT Cuts at: 2582 2663 2582 Size: 81 6664 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6548 AflIII A'CryG_T Cuts at: 1203 2964 3027 5644 6621 1203 Size: 1761 63 2617 977 1327 Fragments arranged by size: 2617 1761 1327 977 63 AgeI A'CCGG_T Cuts at: 5576 5576 Size: 6745 AhdI GACnn_n'nnGTC Cuts at: 5474 5474 Size: 6745 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6745 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3593 1184 Size: 2409 4336 AlwNI CAG_nnn'CTG Cuts at: 3357 4488 6060 3357 Size: 1131 1572 4042 Fragments arranged by size: 4042 1572 1131 ApaLI G'TGCA_C Cuts at: 3074 4802 5958 3074 Size: 1728 1156 3861 Fragments arranged by size: 3861 1728 1156 ApoI r'AATT_y Cuts at: 1493 3008 4054 4280 4432 6541 1493 Size: 1515 1046 226 152 2109 1697 Fragments arranged by size: 2109 1697 1515 1046 226 152 AscI GG'CGCG_CC Cuts at: 2878 2878 Size: 6745 AseI AT'TA_AT Cuts at: 160 1032 5299 160 Size: 872 4267 1606 Fragments arranged by size: 4267 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6745 AvaI C'yCGr_G Cuts at: 1870 1943 2576 2831 3608 4070 1870 Size: 73 633 255 777 462 4545 Fragments arranged by size: 4545 777 633 462 255 73 BamHI G'GATC_C Cuts at: 4075 4442 4075 Size: 367 6378 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2288 2465 2821 3748 4066 Size: 325 217 627 501 177 356 927 318 Cuts at: 4066 5521 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 927 627 501 356 325 318 217 177 BanII G_rGCy'C Cuts at: 1391 2081 2520 2762 3621 4064 1391 Size: 690 439 242 859 443 4072 Fragments arranged by size: 4072 859 690 443 439 242 BbeI G_GCGC'C Cuts at: 2292 2469 2292 Size: 177 6568 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2224 928 Size: 971 325 5449 Fragments arranged by size: 5449 971 325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4936 4970 1903 Size: 34 2999 34 3678 Fragments arranged by size: 3678 2999 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4637 5847 4637 Size: 1210 5535 BclI T'GATC_A Cuts at: 1548 1548 Size: 6745 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2286 2505 3734 Size: 107 122 71 631 605 614 219 1229 Cuts at: 3734 5356 136 Size: 1622 1525 Fragments arranged by size: 1622 1525 1229 631 614 605 219 122 107 71 BglII A'GATC_T Cuts at: 6740 6740 Size: 6745 BlpI GC'TnA_GC Cuts at: 2738 4162 2738 Size: 1424 5321 Bme1580I G_kGCm'C Cuts at: 1790 2634 3078 3582 3609 4806 5962 1790 Size: 844 444 504 27 1197 1156 2573 Fragments arranged by size: 2573 1197 1156 844 504 444 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6745 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4476 5434 442 Size: 416 53 83 399 24 3059 958 1753 Fragments arranged by size: 3059 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6745 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3379 3920 5405 1239 Size: 2140 541 1485 2579 Fragments arranged by size: 2579 2140 1485 541 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3782 4870 5735 6033 3782 Size: 1088 865 298 4494 Fragments arranged by size: 4494 1088 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2838 5408 882 Size: 617 1339 2570 2219 Fragments arranged by size: 2570 2219 1339 617 BsaAI yAC'GTr Cuts at: 493 1204 4536 493 Size: 711 3332 2702 Fragments arranged by size: 3332 2702 711 BsaBI GATnn'nnATC Cuts at: 6739 6739 Size: 6745 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2289 2466 2556 2766 Size: 53 83 186 936 756 177 90 210 Cuts at: 2766 3048 3924 3980 4040 4552 4934 5569 275 Size: 282 876 56 60 512 382 635 1451 Fragments arranged by size: 1451 936 876 756 635 512 382 282 210 186 177 90 83 60 56 53 BsaWI w'CCGG_w Cuts at: 5178 5576 5850 5997 6408 5178 Size: 398 274 147 411 5515 Fragments arranged by size: 5515 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2126 2156 2214 2244 2255 Size: 30 165 30 723 30 58 30 11 Cuts at: 2255 2285 2756 2786 1178 Size: 30 471 30 5137 Fragments arranged by size: 5137 723 471 165 58 30 30 30 30 30 30 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2408 2539 1186 Size: 1222 131 5392 Fragments arranged by size: 5392 1222 131 BseYI C'CCAG_C Cuts at: 2074 2296 2388 2520 3350 3532 3582 5948 6444 Size: 222 92 132 830 182 50 2366 496 Cuts at: 6444 2074 Size: 2375 Fragments arranged by size: 2375 2366 830 496 222 182 132 92 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2315 2912 1163 Size: 573 579 597 4996 Fragments arranged by size: 4996 597 579 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2316 2607 2719 3338 4008 4195 Size: 275 186 315 291 112 619 670 187 Cuts at: 4195 4956 5984 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 670 619 315 291 275 187 186 112 BsiHKAI G_wGCw'C Cuts at: 2424 3078 3519 3621 4806 4891 5962 2424 Size: 654 441 102 1185 85 1071 3207 Fragments arranged by size: 3207 1185 1071 654 441 102 85 BsmI GAATG_Cn' Cuts at: 4256 4349 4256 Size: 93 6652 BsmBI CGTCTCn'nnnn_ Cuts at: 2146 2146 Size: 6745 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2424 2520 2634 2762 3078 3519 Size: 399 291 343 96 114 128 316 441 Cuts at: 3519 3582 3609 3621 4064 4806 4891 5962 1391 Size: 63 27 12 443 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 443 441 399 343 316 291 128 114 96 85 63 27 12 BspEI T'CCGG_A Cuts at: 6408 6408 Size: 6745 BspHI T'CATG_A Cuts at: 4634 6364 4634 Size: 1730 5015 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2044 2218 2983 3169 3252 3318 4100 844 Size: 1200 174 765 186 83 66 782 3489 Fragments arranged by size: 3489 1200 782 765 186 174 83 66 BsrBI CCG'CTC Cuts at: 1394 1823 2250 4632 1394 Size: 429 427 2382 3507 Fragments arranged by size: 3507 2382 429 427 BsrDI GCAATG_nn' Cuts at: 65 1248 5240 5414 65 Size: 1183 3992 174 1396 Fragments arranged by size: 3992 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2714 3050 3153 3333 3363 3593 3655 Size: 110 904 336 103 180 30 230 62 Cuts at: 3655 3938 3982 5389 5576 1700 Size: 283 44 1407 187 2869 Fragments arranged by size: 2869 1407 904 336 283 230 187 180 110 103 62 44 30 BsrGI T'GTAC_A Cuts at: 96 2007 2967 96 Size: 1911 960 3874 Fragments arranged by size: 3874 1911 960 BssHII G'CGCG_C Cuts at: 1925 2256 2599 2681 2878 1925 Size: 331 343 82 197 5792 Fragments arranged by size: 5792 343 331 197 82 BssSI C'ACGA_G Cuts at: 4805 5817 4805 Size: 1012 5733 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6745 BstBI TT'CG_AA Cuts at: 5549 5549 Size: 6745 BstEII G'GTnAC_C Cuts at: 1184 3156 3778 3791 1184 Size: 1972 622 13 4138 Fragments arranged by size: 4138 1972 622 13 BstXI CCAn_nnnn'nTGG Cuts at: 2434 2459 2500 2434 Size: 25 41 6679 Fragments arranged by size: 6679 41 25 BstYI r'GATC_y Cuts at: 1972 2919 4075 4442 4827 4844 6296 6382 6394 Size: 947 1156 367 385 17 1452 86 12 Cuts at: 6394 6740 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1156 947 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2082 2112 2415 2883 3147 3270 3314 Size: 552 1017 30 303 468 264 123 44 Cuts at: 3314 3510 513 Size: 196 3748 Fragments arranged by size: 3748 1017 552 468 303 264 196 123 44 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2298 2553 3615 3694 487 Size: 683 56 1072 255 1062 79 3538 Fragments arranged by size: 3538 1072 1062 683 255 79 56 BtsI GCAGTG_nn' Cuts at: 4261 5055 5075 4261 Size: 794 20 5931 Fragments arranged by size: 5931 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6745 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6710 DraI TTT'AAA Cuts at: 4049 4049 Size: 6745 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3353 5752 809 Size: 507 2037 2399 1802 Fragments arranged by size: 2399 2037 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2313 2506 Size: 54 1425 50 275 18 279 204 193 Cuts at: 2506 2604 2716 3335 3642 3657 3705 4192 5081 Size: 98 112 619 307 15 48 487 889 Cuts at: 5081 6710 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 619 487 307 279 275 204 193 112 98 54 50 48 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2313 2604 2716 3335 4192 1537 Size: 275 501 291 112 619 857 4090 Fragments arranged by size: 4090 857 619 501 291 275 112 EarI CTCTTCn'nnn_ Cuts at: 1353 4675 1353 Size: 3322 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2171 2225 2486 2912 3023 3419 Size: 1064 138 582 54 261 426 111 396 Cuts at: 3419 3440 5332 5706 5852 387 Size: 21 1892 374 146 1280 Fragments arranged by size: 1892 1280 1064 582 426 396 374 261 146 138 111 54 21 EcoICRI GAG'CTC Cuts at: 3619 3619 Size: 6745 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2660 3307 3379 3920 4808 5405 Size: 393 452 576 647 72 541 888 597 Cuts at: 5405 6192 6460 1239 Size: 787 268 1524 Fragments arranged by size: 1524 888 787 647 597 576 541 452 393 268 72 EcoNI CCTnn'n_nnAGG Cuts at: 2150 2150 Size: 6745 EcoRI G'AATT_C Cuts at: 4054 4432 4054 Size: 378 6367 FspI TGC'GCA Cuts at: 5251 5251 Size: 6745 HaeII r_GCGC'y Cuts at: 1678 2292 2405 2469 2584 2665 2732 2870 3370 Size: 614 113 64 115 81 67 138 500 Cuts at: 3370 3386 3688 5892 1678 Size: 16 302 2204 2531 Fragments arranged by size: 2531 2204 614 500 302 138 115 113 81 67 64 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2214 2246 2255 Size: 32 135 32 443 32 167 32 9 Cuts at: 2255 2287 2531 2563 2756 2788 3452 3484 5386 Size: 32 244 32 193 32 664 32 1902 Cuts at: 5386 5418 5460 5492 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1902 664 443 244 193 167 135 42 32 32 32 32 32 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 669 1657 1938 3088 4009 4089 4335 669 Size: 988 281 1150 921 80 246 3079 Fragments arranged by size: 3079 1150 988 921 281 246 80 HindIII A'AGCT_T Cuts at: 748 748 Size: 6745 HpaI GTT'AAC Cuts at: 4335 4335 Size: 6745 KasI G'GCGC_C Cuts at: 2288 2465 2288 Size: 177 6568 KpnI G_GTAC'C Cuts at: 4070 4070 Size: 6745 MfeI C'AATT_G Cuts at: 4344 4344 Size: 6745 MluI A'CGCG_T Cuts at: 6621 6621 Size: 6745 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2577 5859 6043 1305 Size: 1272 3282 184 2007 Fragments arranged by size: 3282 2007 1272 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2508 3707 10 Size: 54 1425 343 676 1199 3048 Fragments arranged by size: 3048 1425 1199 676 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2369 2457 3377 4703 5062 5221 518 Size: 648 1203 88 920 1326 359 159 2042 Fragments arranged by size: 2042 1326 1203 920 648 359 159 88 MspA1I CmG'CkG Cuts at: 1606 2071 2084 2114 2329 3316 3340 3512 3586 Size: 465 13 30 215 987 24 172 74 Cuts at: 3586 3754 4161 4838 5986 6231 1606 Size: 168 407 677 1148 245 2120 Fragments arranged by size: 2120 1148 987 677 465 407 245 215 172 168 74 30 24 13 NaeI GCC'GGC Cuts at: 2716 3052 3365 3595 3657 3940 2716 Size: 336 313 230 62 283 5521 Fragments arranged by size: 5521 336 313 283 230 62 NarI GG'CG_CC Cuts at: 2289 2466 2289 Size: 177 6568 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6193 NdeI CA'TA_TG Cuts at: 387 387 Size: 6745 NgoMIV G'CCGG_C Cuts at: 2714 3050 3363 3593 3655 3938 2714 Size: 336 313 230 62 283 5521 Fragments arranged by size: 5521 336 313 283 230 62 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6745 NotI GC'GGCC_GC Cuts at: 2604 4192 2604 Size: 1588 5157 NruI TCG'CGA Cuts at: 3465 3465 Size: 6745 NspI r_CATG'y Cuts at: 1132 1229 2968 3031 3532 4103 5648 1132 Size: 97 1739 63 501 571 1545 2229 Fragments arranged by size: 2229 1739 1545 571 501 97 63 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6745 PciI A'CATG_T Cuts at: 2964 3027 5644 2964 Size: 63 2617 4065 Fragments arranged by size: 4065 2617 63 PflMI CCAn_nnn'nTGG Cuts at: 1108 3357 1108 Size: 2249 4496 PmeI GTTT'AAAC Cuts at: 4049 4049 Size: 6745 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4814 4846 6327 6359 4814 Size: 32 1481 32 5200 Fragments arranged by size: 5200 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6745 PsiI TTA'TAA Cuts at: 4315 4315 Size: 6745 PspXI vC'TCGA_Gb Cuts at: 2576 2576 Size: 6745 PstI C_TGCA'G Cuts at: 830 2279 4097 830 Size: 1449 1818 3478 Fragments arranged by size: 3478 1818 1449 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6745 SacI G_AGCT'C Cuts at: 3621 3621 Size: 6745 SacII CC_GC'GG Cuts at: 2085 2115 3317 3513 2085 Size: 30 1202 196 5317 Fragments arranged by size: 5317 1202 196 30 SalI G'TCGA_C Cuts at: 1936 4007 4087 1936 Size: 2071 80 4594 Fragments arranged by size: 4594 2071 80 SbfI CC_TGCA'GG Cuts at: 4097 4097 Size: 6745 ScaI AGT'ACT Cuts at: 4993 6705 4993 Size: 1712 5033 SfcI C'TryA_G Cuts at: 826 1045 2134 2275 4093 5228 5909 6100 826 Size: 219 1089 141 1818 1135 681 191 1471 Fragments arranged by size: 1818 1471 1135 1089 681 219 191 141 SfoI GGC'GCC Cuts at: 2290 2467 2290 Size: 177 6568 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6745 SgrAI Cr'CCGG_yG Cuts at: 1700 3982 1700 Size: 2282 4463 SmaI CCC'GGG Cuts at: 1872 4072 1872 Size: 2200 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2576 3761 4849 5750 6012 820 Size: 197 926 633 1185 1088 901 262 1553 Fragments arranged by size: 1553 1185 1088 926 901 633 262 197 SnaBI TAC'GTA Cuts at: 493 4536 493 Size: 4043 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6745 SphI G_CATG'C Cuts at: 1132 3532 4103 1132 Size: 2400 571 3774 Fragments arranged by size: 3774 2400 571 SspI AAT'ATT Cuts at: 5 52 4669 5 Size: 47 4617 2081 Fragments arranged by size: 4617 2081 47 StuI AGG'CCT Cuts at: 1517 2837 1517 Size: 1320 5425 StyI C'CwwG_G Cuts at: 513 1065 2358 4184 513 Size: 552 1293 1826 3074 Fragments arranged by size: 3074 1826 1293 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3061 3228 4772 4789 4942 5127 5572 Size: 1255 1481 167 1544 17 153 185 445 Cuts at: 5572 325 Size: 1498 Fragments arranged by size: 1544 1498 1481 1255 445 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 2784 2967 Size: 274 80 33 51 1443 30 777 183 Cuts at: 2967 4991 6703 96 Size: 2024 1712 138 Fragments arranged by size: 2024 1712 1443 777 274 183 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6705 6737 6705 Size: 32 6713 Tth111I GACn'n_nGTC Cuts at: 1316 2366 1316 Size: 1050 5695 XbaI T'CTAG_A Cuts at: 4081 4081 Size: 6745 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2459 2459 Size: 6745 XhoI C'TCGA_G Cuts at: 1943 2576 1943 Size: 633 6112 XmaI C'CCGG_G Cuts at: 1870 4070 1870 Size: 2200 4545 XmnI GAAnn'nnTTC Cuts at: 4874 4874 Size: 6745 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2767 3925 4553 276 Size: 53 83 186 936 1233 1158 628 2468 Fragments arranged by size: 2468 1233 1158 936 628 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI