Restriction map for
pFN21AA1161
(Linear) (Six-base) MAPSORT of: KIAA1161.seq Check: 8821 from: 1 to: 2040
KIAA1161.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 39 978 1247 2040
Size: 39 939 269 793
Fragments arranged by size:
939 793 269 39
AatII G_ACGT'C
Cuts at: 0 764 1922 2040
Size: 764 1158 118
Fragments arranged by size:
1158 764 118
AccI GT'mk_AC
Cuts at: 0 539 2003 2040
Size: 539 1464 37
Fragments arranged by size:
1464 539 37
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 655 1302 2040
Size: 79 576 647 738
Fragments arranged by size:
738 647 576 79
AfeI AGC'GCT
Cuts at: 0 577 658 2040
Size: 577 81 1382
Fragments arranged by size:
1382 577 81
AflIII A'CryG_T
Cuts at: 0 959 1022 2040
Size: 959 63 1018
Fragments arranged by size:
1018 959 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1588 2040
Size: 1588 452
AlwNI CAG_nnn'CTG
Cuts at: 0 1352 2040
Size: 1352 688
ApaLI G'TGCA_C
Cuts at: 0 1069 2040
Size: 1069 971
ApoI r'AATT_y
Cuts at: 0 1003 2040
Size: 1003 1037
AscI GG'CGCG_CC
Cuts at: 0 873 2040
Size: 873 1167
AvaI C'yCGr_G
Cuts at: 0 571 826 1603 2040
Size: 571 255 777 437
Fragments arranged by size:
777 571 437 255
BanI G'GyrC_C
Cuts at: 0 283 460 816 1743 2040
Size: 283 177 356 927 297
Fragments arranged by size:
927 356 297 283 177
BanII G_rGCy'C
Cuts at: 0 76 515 757 1616 2040
Size: 76 439 242 859 424
Fragments arranged by size:
859 439 424 242 76
BbeI G_GCGC'C
Cuts at: 0 287 464 2040
Size: 287 177 1576
Fragments arranged by size:
1576 287 177
BbsI GAAGACnn'nnnn_
Cuts at: 0 219 2040
Size: 219 1821
BglI GCCn_nnn'nGGC
Cuts at: 0 281 500 1729 2040
Size: 281 219 1229 311
Fragments arranged by size:
1229 311 281 219
BlpI GC'TnA_GC
Cuts at: 0 733 2040
Size: 733 1307
Bme1580I G_kGCm'C
Cuts at: 0 629 1073 1577 1604 2040
Size: 629 444 504 27 436
Fragments arranged by size:
629 504 444 436 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1374 1915 2040
Size: 1374 541 125
Fragments arranged by size:
1374 541 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1777 2040
Size: 1777 263
BsaI GGTCTCn'nnnn_
Cuts at: 0 833 2040
Size: 833 1207
BsaHI Gr'CG_yC
Cuts at: 0 284 461 551 761 1043 1919 1975 2035
Size: 284 177 90 210 282 876 56 60
Cuts at: 2035 2040
Size: 5
Fragments arranged by size:
876 284 282 210 177 90 60 56
5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 121 151 209 239 250 280 751 781
Size: 121 30 58 30 11 30 471 30
Cuts at: 781 2040
Size: 1259
Fragments arranged by size:
1259 471 121 58 30 30 30 30
11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 403 534 2040
Size: 403 131 1506
Fragments arranged by size:
1506 403 131
BseYI C'CCAG_C
Cuts at: 0 69 291 383 515 1345 1527 1577 2040
Size: 69 222 92 132 830 182 50 463
Fragments arranged by size:
830 463 222 182 132 92 69 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 907 2040
Size: 310 597 1133
Fragments arranged by size:
1133 597 310
BsiEI CG_ry'CG
Cuts at: 0 311 602 714 1333 2003 2040
Size: 311 291 112 619 670 37
Fragments arranged by size:
670 619 311 291 112 37
BsiHKAI G_wGCw'C
Cuts at: 0 419 1073 1514 1616 2040
Size: 419 654 441 102 424
Fragments arranged by size:
654 441 424 419 102
BsmBI CGTCTCn'nnnn_
Cuts at: 0 141 2040
Size: 141 1899
Bsp1286I G_dGCh'C
Cuts at: 0 76 419 515 629 757 1073 1514 1577
Size: 76 343 96 114 128 316 441 63
Cuts at: 1577 1604 1616 2040
Size: 27 12 424
Fragments arranged by size:
441 424 343 316 128 114 96 76
63 27 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 39 213 978 1164 1247 1313 2040
Size: 39 174 765 186 83 66 727
Fragments arranged by size:
765 727 186 174 83 66 39
BsrBI CCG'CTC
Cuts at: 0 245 2040
Size: 245 1795
BsrFI r'CCGG_y
Cuts at: 0 709 1045 1148 1328 1358 1588 1650 1933
Size: 709 336 103 180 30 230 62 283
Cuts at: 1933 1977 2040
Size: 44 63
Fragments arranged by size:
709 336 283 230 180 103 63 62
44 30
BsrGI T'GTAC_A
Cuts at: 0 2 962 2040
Size: 2 960 1078
Fragments arranged by size:
1078 960 2
BssHII G'CGCG_C
Cuts at: 0 251 594 676 873 2040
Size: 251 343 82 197 1167
Fragments arranged by size:
1167 343 251 197 82
BstEII G'GTnAC_C
Cuts at: 0 1151 1773 1786 2040
Size: 1151 622 13 254
Fragments arranged by size:
1151 622 254 13
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 454 495 2040
Size: 429 25 41 1545
Fragments arranged by size:
1545 429 41 25
BstYI r'GATC_y
Cuts at: 0 914 2040
Size: 914 1126
BtgI C'CryG_G
Cuts at: 0 77 107 410 878 1142 1265 1309 1505
Size: 77 30 303 468 264 123 44 196
Cuts at: 1505 2040
Size: 535
Fragments arranged by size:
535 468 303 264 196 123 77 44
30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 293 548 1610 1689 2040
Size: 293 255 1062 79 351
Fragments arranged by size:
1062 351 293 255 79
DrdI GACnn_nn'nnGTC
Cuts at: 0 1348 2040
Size: 1348 692
EaeI y'GGCC_r
Cuts at: 0 104 308 501 599 711 1330 1637 1652
Size: 104 204 193 98 112 619 307 15
Cuts at: 1652 1700 2040
Size: 48 340
Fragments arranged by size:
619 340 307 204 193 112 104 98
48 15
EagI C'GGCC_G
Cuts at: 0 308 599 711 1330 2040
Size: 308 291 112 619 710
Fragments arranged by size:
710 619 308 291 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 166 220 481 907 1018 1414 1435 2040
Size: 166 54 261 426 111 396 21 605
Fragments arranged by size:
605 426 396 261 166 111 54 21
EcoICRI GAG'CTC
Cuts at: 0 1614 2040
Size: 1614 426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 655 1302 1374 1915 2040
Size: 79 576 647 72 541 125
Fragments arranged by size:
647 576 541 125 79 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 2040
Size: 145 1895
HaeII r_GCGC'y
Cuts at: 0 287 400 464 579 660 727 865 1365
Size: 287 113 64 115 81 67 138 500
Cuts at: 1365 1381 1683 2040
Size: 16 302 357
Fragments arranged by size:
500 357 302 287 138 115 113 81
67 64 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 209 241 250 282 526 558
Size: 10 32 167 32 9 32 244 32
Cuts at: 558 751 783 1447 1479 2040
Size: 193 32 664 32 561
Fragments arranged by size:
664 561 244 193 167 32 32 32
32 32 32 10 9
HincII GTy'rAC
Cuts at: 0 1083 2004 2040
Size: 1083 921 36
Fragments arranged by size:
1083 921 36
KasI G'GCGC_C
Cuts at: 0 283 460 2040
Size: 283 177 1580
Fragments arranged by size:
1580 283 177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 2040
Size: 572 1468
MscI TGG'CCA
Cuts at: 0 503 1702 2040
Size: 503 1199 338
Fragments arranged by size:
1199 503 338
MslI CAynn'nnrTG
Cuts at: 0 364 452 1372 2040
Size: 364 88 920 668
Fragments arranged by size:
920 668 364 88
MspA1I CmG'CkG
Cuts at: 0 66 79 109 324 1311 1335 1507 1581
Size: 66 13 30 215 987 24 172 74
Cuts at: 1581 1749 2040
Size: 168 291
Fragments arranged by size:
987 291 215 172 168 74 66 30
24 13
NaeI GCC'GGC
Cuts at: 0 711 1047 1360 1590 1652 1935 2040
Size: 711 336 313 230 62 283 105
Fragments arranged by size:
711 336 313 283 230 105 62
NarI GG'CG_CC
Cuts at: 0 284 461 2040
Size: 284 177 1579
Fragments arranged by size:
1579 284 177
NgoMIV G'CCGG_C
Cuts at: 0 709 1045 1358 1588 1650 1933 2040
Size: 709 336 313 230 62 283 107
Fragments arranged by size:
709 336 313 283 230 107 62
NotI GC'GGCC_GC
Cuts at: 0 599 2040
Size: 599 1441
NruI TCG'CGA
Cuts at: 0 1460 2040
Size: 1460 580
NspI r_CATG'y
Cuts at: 0 963 1026 1527 2040
Size: 963 63 501 513
Fragments arranged by size:
963 513 501 63
PciI A'CATG_T
Cuts at: 0 959 1022 2040
Size: 959 63 1018
Fragments arranged by size:
1018 959 63
PflMI CCAn_nnn'nTGG
Cuts at: 0 1352 2040
Size: 1352 688
PspXI vC'TCGA_Gb
Cuts at: 0 571 2040
Size: 571 1469
PstI C_TGCA'G
Cuts at: 0 274 2040
Size: 274 1766
SacI G_AGCT'C
Cuts at: 0 1616 2040
Size: 1616 424
SacII CC_GC'GG
Cuts at: 0 80 110 1312 1508 2040
Size: 80 30 1202 196 532
Fragments arranged by size:
1202 532 196 80 30
SalI G'TCGA_C
Cuts at: 0 2002 2040
Size: 2002 38
SfcI C'TryA_G
Cuts at: 0 129 270 2040
Size: 129 141 1770
Fragments arranged by size:
1770 141 129
SfoI GGC'GCC
Cuts at: 0 285 462 2040
Size: 285 177 1578
Fragments arranged by size:
1578 285 177
SgrAI Cr'CCGG_yG
Cuts at: 0 1977 2040
Size: 1977 63
SmlI C'TyrA_G
Cuts at: 0 571 1756 2040
Size: 571 1185 284
Fragments arranged by size:
1185 571 284
SphI G_CATG'C
Cuts at: 0 1527 2040
Size: 1527 513
StuI AGG'CCT
Cuts at: 0 832 2040
Size: 832 1208
StyI C'CwwG_G
Cuts at: 0 353 2040
Size: 353 1687
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1056 1223 2040
Size: 1056 167 817
Fragments arranged by size:
1056 817 167
TatI w'GTAC_w
Cuts at: 0 2 779 962 2040
Size: 2 777 183 1078
Fragments arranged by size:
1078 777 183 2
Tth111I GACn'n_nGTC
Cuts at: 0 361 2040
Size: 361 1679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 454 2040
Size: 454 1586
XhoI C'TCGA_G
Cuts at: 0 571 2040
Size: 571 1469
ZraI GAC'GTC
Cuts at: 0 762 1920 2040
Size: 762 1158 120
Fragments arranged by size:
1158 762 120
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AlfI AlwNI
ApaLI ApoI AscI AvaI BanI BanII BbeI BbsI
BglI BlpI Bme1580I BpmI BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssHII BstEII BstXI BstYI BtgI
BtgZI DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NotI NruI NspI PciI PflMI
PspXI PstI SacI SacII SalI SfcI SfoI SgrAI
SmlI SphI StuI StyI TaqII TatI Tth111I XcmI
XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AleI AloI ApaI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I
BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI
BssSI BstAPI BstBI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII EarI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI NcoI
NdeI NheI NsiI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI PvuII
RsrII SanDI SapI SbfI ScaI SexAI SfiI SgfI
SmaI SnaBI SpeI SrfI SspI SwaI TstI XbaI
XmaI XmnI