(Circular) (Six-base) MAPSORT of: pFN21AA1145.seq Check: 6257 from: 1 to: 6043 pFN21AA1145 6043 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3853 278 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3386 1937 Size: 1449 4594 Acc65I G'GTAC_C Cuts at: 3364 3364 Size: 6043 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 2411 2864 2993 4106 5490 5758 1632 Size: 512 267 453 129 1113 1384 268 1917 Fragments arranged by size: 1917 1384 1113 512 453 268 267 129 AfeI AGC'GCT Cuts at: 2638 3026 2638 Size: 388 5655 AflII C'TTAA_G Cuts at: 820 1017 2014 820 Size: 197 997 4849 Fragments arranged by size: 4849 997 197 AflIII A'CryG_T Cuts at: 1203 4942 5919 1203 Size: 3739 977 1327 Fragments arranged by size: 3739 1327 977 AgeI A'CCGG_T Cuts at: 4874 4874 Size: 6043 AhdI GACnn_n'nnGTC Cuts at: 4772 4772 Size: 6043 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6043 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6043 AlwNI CAG_nnn'CTG Cuts at: 2887 2983 3786 5358 2887 Size: 96 803 1572 3572 Fragments arranged by size: 3572 1572 803 96 ApaLI G'TGCA_C Cuts at: 4100 5256 4100 Size: 1156 4887 ApoI r'AATT_y Cuts at: 1493 2133 2584 3352 3578 3730 5839 1493 Size: 640 451 768 226 152 2109 1697 Fragments arranged by size: 2109 1697 768 640 451 226 152 AseI AT'TA_AT Cuts at: 160 1032 4597 160 Size: 872 3565 1606 Fragments arranged by size: 3565 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 3368 1870 Size: 73 1425 4545 Fragments arranged by size: 4545 1425 73 BamHI G'GATC_C Cuts at: 3373 3740 3373 Size: 367 5676 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3254 3364 4819 618 Size: 325 217 627 1467 110 1455 1842 Fragments arranged by size: 1842 1467 1455 627 325 217 110 BanII G_rGCy'C Cuts at: 1391 2366 3087 3362 1391 Size: 975 721 275 4072 Fragments arranged by size: 4072 975 721 275 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5072 BbvCI CC'TCA_GC Cuts at: 2826 2826 Size: 6043 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2303 2337 2552 2586 4234 4268 1903 Size: 34 366 34 215 34 1648 34 3678 Fragments arranged by size: 3678 1648 366 215 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2800 3935 5145 2800 Size: 1135 1210 3698 Fragments arranged by size: 3698 1210 1135 BclI T'GATC_A Cuts at: 1548 1548 Size: 6043 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3014 4654 136 Size: 107 122 71 631 605 1342 1640 1525 Fragments arranged by size: 1640 1525 1342 631 605 122 107 71 BglII A'GATC_T Cuts at: 6038 6038 Size: 6043 BlpI GC'TnA_GC Cuts at: 2817 3460 2817 Size: 643 5400 Bme1580I G_kGCm'C Cuts at: 1790 4104 5260 1790 Size: 2314 1156 2573 Fragments arranged by size: 2573 2314 1156 BmgBI CAC'GTC Cuts at: 1318 2694 1318 Size: 1376 4667 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3307 3774 4732 Size: 416 53 83 399 24 1890 467 958 Cuts at: 4732 442 Size: 1753 Fragments arranged by size: 1890 1753 958 467 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2804 2939 3101 4703 1239 Size: 1565 135 162 1602 2579 Fragments arranged by size: 2579 1602 1565 162 135 Bpu10I CC'TnA_GC Cuts at: 2826 2826 Size: 6043 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4168 5033 5331 4168 Size: 865 298 4880 Fragments arranged by size: 4880 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2488 4706 882 Size: 617 989 2218 2219 Fragments arranged by size: 2219 2218 989 617 BsaAI yAC'GTr Cuts at: 493 1204 2425 3834 493 Size: 711 1221 1409 2702 Fragments arranged by size: 2702 1409 1221 711 BsaBI GATnn'nnATC Cuts at: 2400 6037 2400 Size: 3637 2406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3850 4232 4867 275 Size: 53 83 186 936 2317 382 635 1451 Fragments arranged by size: 2317 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2541 4476 4874 5148 5295 5706 2541 Size: 1935 398 274 147 411 2878 Fragments arranged by size: 2878 1935 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5818 Fragments arranged by size: 5818 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2609 3011 1186 Size: 1423 402 4218 Fragments arranged by size: 4218 1423 402 BseYI C'CCAG_C Cuts at: 2292 5246 5742 2292 Size: 2954 496 2593 Fragments arranged by size: 2954 2593 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2870 1163 Size: 573 1134 4336 Fragments arranged by size: 4336 1134 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2706 3493 4254 5282 1540 Size: 275 186 705 787 761 1028 2301 Fragments arranged by size: 2301 1028 787 761 705 275 186 BsiHKAI G_wGCw'C Cuts at: 2366 3087 4104 4189 5260 2366 Size: 721 1017 85 1071 3149 Fragments arranged by size: 3149 1071 1017 721 85 BsmI GAATG_Cn' Cuts at: 3133 3554 3647 3133 Size: 421 93 5529 Fragments arranged by size: 5529 421 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3223 3223 Size: 6043 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2366 3087 3362 4104 4189 5260 1391 Size: 399 576 721 275 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 721 576 399 275 85 BspEI T'CCGG_A Cuts at: 2541 5706 2541 Size: 3165 2878 BspHI T'CATG_A Cuts at: 3174 3932 5662 3174 Size: 758 1730 3555 Fragments arranged by size: 3555 1730 758 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2955 3398 844 Size: 2111 443 3489 Fragments arranged by size: 3489 2111 443 BsrBI CCG'CTC Cuts at: 1394 1823 3930 1394 Size: 429 2107 3507 Fragments arranged by size: 3507 2107 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2446 2568 4538 4712 65 Size: 1183 1198 122 1970 174 1396 Fragments arranged by size: 1970 1396 1198 1183 174 122 BsrFI r'CCGG_y Cuts at: 1700 1810 4687 4874 1700 Size: 110 2877 187 2869 Fragments arranged by size: 2877 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6043 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6043 BssSI C'ACGA_G Cuts at: 4103 5115 4103 Size: 1012 5031 BstAPI GCAn_nnn'nTGC Cuts at: 794 3109 794 Size: 2315 3728 BstBI TT'CG_AA Cuts at: 4847 4847 Size: 6043 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6043 BstXI CCAn_nnnn'nTGG Cuts at: 2729 2752 3251 2729 Size: 23 499 5521 Fragments arranged by size: 5521 499 23 BstYI r'GATC_y Cuts at: 1972 3373 3740 4125 4142 5594 5680 5692 6038 Size: 1401 367 385 17 1452 86 12 346 Cuts at: 6038 1972 Size: 1977 Fragments arranged by size: 1977 1452 1401 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5491 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2157 2193 2272 2387 3190 487 Size: 683 56 931 36 79 115 803 3340 Fragments arranged by size: 3340 931 803 683 115 79 56 36 BtsI GCAGTG_nn' Cuts at: 2109 2674 3559 4353 4373 2109 Size: 565 885 794 20 3779 Fragments arranged by size: 3779 885 794 565 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6008 DraI TTT'AAA Cuts at: 3347 3347 Size: 6043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5050 809 Size: 507 3734 1802 Fragments arranged by size: 3734 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2703 2985 3490 Size: 54 1425 50 275 18 873 282 505 Cuts at: 3490 4379 6008 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 873 505 282 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2703 3490 1537 Size: 275 891 787 4090 Fragments arranged by size: 4090 891 787 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3227 3279 3973 1353 Size: 1874 52 694 3423 Fragments arranged by size: 3423 1874 694 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4630 5004 5150 387 Size: 1064 138 3041 374 146 1280 Fragments arranged by size: 3041 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2364 3085 2364 Size: 721 5322 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2411 2804 2864 2939 2993 3101 Size: 393 512 267 393 60 75 54 108 Cuts at: 3101 4106 4703 5490 5758 1239 Size: 1005 597 787 268 1524 Fragments arranged by size: 1524 1005 787 597 512 393 393 268 267 108 75 60 54 EcoNI CCTnn'n_nnAGG Cuts at: 2891 2975 2891 Size: 84 5959 EcoO109I rG'GnC_Cy Cuts at: 2622 2622 Size: 6043 EcoRI G'AATT_C Cuts at: 3352 3730 3352 Size: 378 5665 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2269 2269 Size: 6043 FspI TGC'GCA Cuts at: 4549 4549 Size: 6043 HaeII r_GCGC'y Cuts at: 1678 2562 2640 3028 5190 1678 Size: 884 78 388 2162 2531 Fragments arranged by size: 2531 2162 884 388 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 3220 3252 4684 Size: 32 135 32 584 32 1032 32 1432 Cuts at: 4684 4716 4758 4790 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1432 1032 584 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3387 3633 669 Size: 988 281 1449 246 3079 Fragments arranged by size: 3079 1449 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6043 HpaI GTT'AAC Cuts at: 3633 3633 Size: 6043 KpnI G_GTAC'C Cuts at: 3368 3368 Size: 6043 MfeI C'AATT_G Cuts at: 3642 3642 Size: 6043 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5157 5341 1305 Size: 3852 184 2007 Fragments arranged by size: 3852 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2987 10 Size: 54 1425 343 1155 3066 Fragments arranged by size: 3066 1425 1155 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2955 3158 3188 4001 4360 4519 518 Size: 648 1789 203 30 813 359 159 2042 Fragments arranged by size: 2042 1789 813 648 359 203 159 30 MspA1I CmG'CkG Cuts at: 1606 2296 2821 2929 3459 4136 5284 5529 1606 Size: 690 525 108 530 677 1148 245 2120 Fragments arranged by size: 2120 1148 690 677 530 525 245 108 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5491 NdeI CA'TA_TG Cuts at: 387 387 Size: 6043 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6043 NotI GC'GGCC_GC Cuts at: 3490 3490 Size: 6043 NruI TCG'CGA Cuts at: 3209 3209 Size: 6043 NspI r_CATG'y Cuts at: 1132 1229 2476 3401 4946 1132 Size: 97 1247 925 1545 2229 Fragments arranged by size: 2229 1545 1247 925 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6043 PciI A'CATG_T Cuts at: 4942 4942 Size: 6043 PflMI CCAn_nnn'nTGG Cuts at: 1108 2452 1108 Size: 1344 4699 PfoI T'CCnGG_A Cuts at: 2073 2085 2781 3039 2073 Size: 12 696 258 5077 Fragments arranged by size: 5077 696 258 12 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PmlI CAC'GTG Cuts at: 2425 2425 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5625 5657 4112 Size: 32 1481 32 4498 Fragments arranged by size: 4498 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6043 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PstI C_TGCA'G Cuts at: 830 2116 2301 3114 3395 830 Size: 1286 185 813 281 3478 Fragments arranged by size: 3478 1286 813 281 185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 PvuII CAG'CTG Cuts at: 2296 2821 2929 2296 Size: 525 108 5410 Fragments arranged by size: 5410 525 108 SacI G_AGCT'C Cuts at: 2366 3087 2366 Size: 721 5322 SalI G'TCGA_C Cuts at: 1936 3385 1936 Size: 1449 4594 SbfI CC_TGCA'GG Cuts at: 3395 3395 Size: 6043 ScaI AGT'ACT Cuts at: 4291 6003 4291 Size: 1712 4331 SfcI C'TryA_G Cuts at: 826 1045 2112 2297 3110 3391 4526 5207 5398 Size: 219 1067 185 813 281 1135 681 191 Cuts at: 5398 826 Size: 1471 Fragments arranged by size: 1471 1135 1067 813 681 281 219 191 185 SfiI GGCCn_nnn'nGGCC Cuts at: 3014 3014 Size: 6043 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6043 SmaI CCC'GGG Cuts at: 1872 3370 1872 Size: 1498 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2014 4147 5048 5310 820 Size: 197 926 71 2133 901 262 1553 Fragments arranged by size: 2133 1553 926 901 262 197 71 SnaBI TAC'GTA Cuts at: 493 3834 493 Size: 3341 2702 SpeI A'CTAG_T Cuts at: 152 2206 152 Size: 2054 3989 SphI G_CATG'C Cuts at: 1132 2476 3401 1132 Size: 1344 925 3774 Fragments arranged by size: 3774 1344 925 SspI AAT'ATT Cuts at: 5 52 3967 5 Size: 47 3915 2081 Fragments arranged by size: 3915 2081 47 StuI AGG'CCT Cuts at: 1517 3017 1517 Size: 1500 4543 StyI C'CwwG_G Cuts at: 513 1065 3048 3482 513 Size: 552 1983 434 3074 Fragments arranged by size: 3074 1983 552 434 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3238 4070 4087 4240 4425 4870 325 Size: 1255 1658 832 17 153 185 445 1498 Fragments arranged by size: 1658 1498 1255 832 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4289 6001 96 Size: 274 80 33 51 1443 2312 1712 138 Fragments arranged by size: 2312 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6003 6035 6003 Size: 32 6011 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6043 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6043 XmaI C'CCGG_G Cuts at: 1870 3368 1870 Size: 1498 4545 XmnI GAAnn'nnTTC Cuts at: 4172 4172 Size: 6043 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3851 276 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI