(Circular) (Six-base) MAPSORT of: pFN21AA1144.seq Check: 8624 from: 1 to: 6136 pFN21AA1144 6136 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2199 3946 278 Size: 53 83 186 936 663 1747 2468 Fragments arranged by size: 2468 1747 936 663 186 83 53 AccI GT'mk_AC Cuts at: 1937 3479 1937 Size: 1542 4594 Acc65I G'GTAC_C Cuts at: 3457 3457 Size: 6136 AclI AA'CG_TT Cuts at: 1563 4263 4636 1563 Size: 2700 373 3063 Fragments arranged by size: 3063 2700 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2290 2554 4199 5583 5851 1632 Size: 658 264 1645 1384 268 1917 Fragments arranged by size: 1917 1645 1384 658 268 264 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5939 AflIII A'CryG_T Cuts at: 1203 2028 3433 5035 6012 1203 Size: 825 1405 1602 977 1327 Fragments arranged by size: 1602 1405 1327 977 825 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 2800 4865 2800 Size: 2065 4071 AleI CACnn'nnGTG Cuts at: 1166 2828 1166 Size: 1662 4474 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6136 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2666 2698 2666 Size: 32 6104 AlwNI CAG_nnn'CTG Cuts at: 2020 2893 3879 5451 2020 Size: 873 986 1572 2705 Fragments arranged by size: 2705 1572 986 873 ApaLI G'TGCA_C Cuts at: 4193 5349 4193 Size: 1156 4980 ApoI r'AATT_y Cuts at: 1493 3445 3671 3823 5932 1493 Size: 1952 226 152 2109 1697 Fragments arranged by size: 2109 1952 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4690 160 Size: 872 3658 1606 Fragments arranged by size: 3658 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 2120 2393 3461 1870 Size: 73 177 273 1068 4545 Fragments arranged by size: 4545 1068 273 177 73 AvrII C'CTAG_G Cuts at: 2678 2678 Size: 6136 BamHI G'GATC_C Cuts at: 3466 3833 3466 Size: 367 5769 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3258 3457 4912 618 Size: 325 217 627 1471 199 1455 1842 Fragments arranged by size: 1842 1471 1455 627 325 217 199 BanII G_rGCy'C Cuts at: 1391 2178 2235 2394 3401 3455 1391 Size: 787 57 159 1007 54 4072 Fragments arranged by size: 4072 1007 787 159 57 54 BbeI G_GCGC'C Cuts at: 3262 3262 Size: 6136 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2290 2433 2560 928 Size: 971 391 143 127 4504 Fragments arranged by size: 4504 971 391 143 127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4327 4361 1903 Size: 34 2390 34 3678 Fragments arranged by size: 3678 2390 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 1548 Size: 6136 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4747 136 Size: 107 122 71 631 605 3075 1525 Fragments arranged by size: 3075 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6131 6131 Size: 6136 BlpI GC'TnA_GC Cuts at: 2286 2558 3553 2286 Size: 272 995 4869 Fragments arranged by size: 4869 995 272 Bme1580I G_kGCm'C Cuts at: 1790 4197 5353 1790 Size: 2407 1156 2573 Fragments arranged by size: 2573 2407 1156 BmgBI CAC'GTC Cuts at: 1318 2029 2278 2439 1318 Size: 711 249 161 5015 Fragments arranged by size: 5015 711 249 161 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2335 3867 4825 Size: 416 53 83 399 24 918 1532 958 Cuts at: 4825 442 Size: 1753 Fragments arranged by size: 1753 1532 958 918 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 4796 1239 Size: 953 2604 2579 Fragments arranged by size: 2604 2579 953 Bpu10I CC'TnA_GC Cuts at: 2229 2229 Size: 6136 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2747 3293 4261 5126 5424 2747 Size: 546 968 865 298 3459 Fragments arranged by size: 3459 968 865 546 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4799 882 Size: 617 3300 2219 Fragments arranged by size: 3300 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2245 3434 3927 493 Size: 711 1041 1189 493 2702 Fragments arranged by size: 2702 1189 1041 711 493 BsaBI GATnn'nnATC Cuts at: 2063 2906 3026 6130 2063 Size: 843 120 3104 2069 Fragments arranged by size: 3104 2069 843 120 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2196 2475 3259 3943 Size: 53 83 186 936 663 279 784 684 Cuts at: 3943 4325 4960 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 784 684 663 635 382 279 186 83 53 BsaWI w'CCGG_w Cuts at: 4569 4967 5241 5388 5799 4569 Size: 398 274 147 411 4906 Fragments arranged by size: 4906 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2306 2336 2462 2492 1178 Size: 30 165 30 903 30 126 30 4822 Fragments arranged by size: 4822 903 165 126 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2101 3077 1186 Size: 915 976 4245 Fragments arranged by size: 4245 976 915 BseYI C'CCAG_C Cuts at: 5339 5835 5339 Size: 496 5640 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5563 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2221 3586 4347 5375 1540 Size: 275 186 220 1365 761 1028 2301 Fragments arranged by size: 2301 1365 1028 761 275 220 186 BsiHKAI G_wGCw'C Cuts at: 2178 2235 2435 2700 2855 3401 4197 4282 5353 Size: 57 200 265 155 546 796 85 1071 Cuts at: 5353 2178 Size: 2961 Fragments arranged by size: 2961 1071 796 546 265 200 155 85 57 BsmI GAATG_Cn' Cuts at: 2782 3647 3740 2782 Size: 865 93 5178 Fragments arranged by size: 5178 865 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2117 2117 Size: 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2178 2235 2394 2435 2700 2855 3401 Size: 399 388 57 159 41 265 155 546 Cuts at: 3401 3455 4197 4282 5353 1391 Size: 54 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 546 399 388 265 159 155 85 57 54 41 BspEI T'CCGG_A Cuts at: 5799 5799 Size: 6136 BspHI T'CATG_A Cuts at: 4025 5755 4025 Size: 1730 4406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3491 844 Size: 2647 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2952 4023 1394 Size: 429 1129 1071 3507 Fragments arranged by size: 3507 1129 1071 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2706 4631 4805 65 Size: 1183 1458 1925 174 1396 Fragments arranged by size: 1925 1458 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2264 3255 4780 4967 1700 Size: 110 199 255 991 1525 187 2869 Fragments arranged by size: 2869 1525 991 255 199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6136 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6136 BssSI C'ACGA_G Cuts at: 3203 4196 5208 3203 Size: 993 1012 4131 Fragments arranged by size: 4131 1012 993 BstAPI GCAn_nnn'nTGC Cuts at: 794 2107 794 Size: 1313 4823 BstBI TT'CG_AA Cuts at: 2685 4940 2685 Size: 2255 3881 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6136 BstXI CCAn_nnnn'nTGG Cuts at: 2590 2590 Size: 6136 BstYI r'GATC_y Cuts at: 1972 2058 2454 2907 3466 3833 4218 4235 5687 Size: 86 396 453 559 367 385 17 1452 Cuts at: 5687 5773 5785 6131 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 559 453 396 385 367 346 86 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2894 2894 Size: 6136 BtgI C'CryG_G Cuts at: 513 1065 2374 3015 3036 3156 513 Size: 552 1309 641 21 120 3493 Fragments arranged by size: 3493 1309 641 552 120 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2196 487 Size: 683 56 970 4427 Fragments arranged by size: 4427 970 683 56 BtsI GCAGTG_nn' Cuts at: 3652 4446 4466 3652 Size: 794 20 5322 Fragments arranged by size: 5322 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6101 DraI TTT'AAA Cuts at: 3420 3440 3420 Size: 20 6116 DraIII CAC_nnn'GTG Cuts at: 2616 3407 2616 Size: 791 5345 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5143 809 Size: 507 3827 1802 Fragments arranged by size: 3827 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 2377 2733 Size: 54 1425 50 275 18 181 366 356 Cuts at: 2733 2928 3078 3583 4472 6101 8 Size: 195 150 505 889 1629 43 Fragments arranged by size: 1629 1425 889 505 366 356 275 195 181 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3583 1537 Size: 275 1771 4090 Fragments arranged by size: 4090 1771 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2240 4066 1353 Size: 887 1826 3423 Fragments arranged by size: 3423 1826 887 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4723 5097 5243 387 Size: 1064 138 3134 374 146 1280 Fragments arranged by size: 3134 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2176 2233 3399 2176 Size: 57 1166 4913 Fragments arranged by size: 4913 1166 57 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2290 2554 4199 4796 5583 5851 Size: 393 560 98 264 1645 597 787 268 Cuts at: 5851 1239 Size: 1524 Fragments arranged by size: 1645 1524 787 597 560 393 268 264 98 EcoO109I rG'GnC_Cy Cuts at: 2674 2890 3073 3319 2674 Size: 216 183 246 5491 Fragments arranged by size: 5491 246 216 183 EcoRI G'AATT_C Cuts at: 3445 3823 3445 Size: 378 5758 FspI TGC'GCA Cuts at: 3287 4642 3287 Size: 1355 4781 HaeII r_GCGC'y Cuts at: 1678 2534 3262 5283 1678 Size: 856 728 2021 2531 Fragments arranged by size: 2531 2021 856 728 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2786 2818 4777 4809 4851 Size: 32 135 32 1214 32 1959 32 42 Cuts at: 4851 4883 1373 Size: 32 2626 Fragments arranged by size: 2626 1959 1214 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3480 3726 669 Size: 988 281 1542 246 3079 Fragments arranged by size: 3079 1542 988 281 246 HindIII A'AGCT_T Cuts at: 748 2270 748 Size: 1522 4614 HpaI GTT'AAC Cuts at: 3726 3726 Size: 6136 KasI G'GCGC_C Cuts at: 3258 3258 Size: 6136 KpnI G_GTAC'C Cuts at: 3461 3461 Size: 6136 MfeI C'AATT_G Cuts at: 3735 3735 Size: 6136 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2013 5250 5434 1305 Size: 708 3237 184 2007 Fragments arranged by size: 3237 2007 708 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2735 2930 3080 10 Size: 54 1425 343 903 195 150 3066 Fragments arranged by size: 3066 1425 903 343 195 150 54 MslI CAynn'nnrTG Cuts at: 518 1166 2069 2588 2612 2828 3203 4094 4453 Size: 648 903 519 24 216 375 891 359 Cuts at: 4453 4612 518 Size: 159 2042 Fragments arranged by size: 2042 903 891 648 519 375 359 216 159 24 MspA1I CmG'CkG Cuts at: 1606 2203 2521 3291 3552 4229 5377 5622 1606 Size: 597 318 770 261 677 1148 245 2120 Fragments arranged by size: 2120 1148 770 677 597 318 261 245 NarI GG'CG_CC Cuts at: 3259 3259 Size: 6136 NcoI C'CATG_G Cuts at: 513 1065 2374 513 Size: 552 1309 4275 Fragments arranged by size: 4275 1309 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 3583 3583 Size: 6136 NspI r_CATG'y Cuts at: 1132 1229 3494 5039 1132 Size: 97 2265 1545 2229 Fragments arranged by size: 2265 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2956 3149 1348 Size: 1608 193 4335 Fragments arranged by size: 4335 1608 193 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6136 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4205 4237 5718 5750 4205 Size: 32 1481 32 4591 Fragments arranged by size: 4591 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2674 2890 3319 2674 Size: 216 429 5491 Fragments arranged by size: 5491 429 216 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6136 PsiI TTA'TAA Cuts at: 3706 3706 Size: 6136 PstI C_TGCA'G Cuts at: 830 2363 3488 830 Size: 1533 1125 3478 Fragments arranged by size: 3478 1533 1125 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6136 PvuII CAG'CTG Cuts at: 2521 3291 2521 Size: 770 5366 SacI G_AGCT'C Cuts at: 2178 2235 3401 2178 Size: 57 1166 4913 Fragments arranged by size: 4913 1166 57 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SapI GCTCTTCn'nnn_ Cuts at: 2240 2240 Size: 6136 SbfI CC_TGCA'GG Cuts at: 3488 3488 Size: 6136 ScaI AGT'ACT Cuts at: 2324 4384 6096 2324 Size: 2060 1712 2364 Fragments arranged by size: 2364 2060 1712 SexAI A'CCwGG_T Cuts at: 2838 2838 Size: 6136 SfcI C'TryA_G Cuts at: 826 1045 2359 2365 3484 4619 5300 5491 826 Size: 219 1314 6 1119 1135 681 191 1471 Fragments arranged by size: 1471 1314 1135 1119 681 219 191 6 SfoI GGC'GCC Cuts at: 3260 3260 Size: 6136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6136 SmaI CCC'GGG Cuts at: 1872 3463 1872 Size: 1591 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2762 3272 4240 5141 5403 820 Size: 197 926 819 510 968 901 262 1553 Fragments arranged by size: 1553 968 926 901 819 510 262 197 SnaBI TAC'GTA Cuts at: 493 3927 493 Size: 3434 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6136 SphI G_CATG'C Cuts at: 1132 3494 1132 Size: 2362 3774 SspI AAT'ATT Cuts at: 5 52 4060 5 Size: 47 4008 2081 Fragments arranged by size: 4008 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6136 StyI C'CwwG_G Cuts at: 513 1065 2374 2678 3575 513 Size: 552 1309 304 897 3074 Fragments arranged by size: 3074 1309 897 552 304 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3316 4163 4180 4333 4518 4963 325 Size: 1255 1736 847 17 153 185 445 1498 Fragments arranged by size: 1736 1498 1255 847 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 4382 6094 Size: 274 80 33 51 1443 345 2060 1712 Cuts at: 6094 96 Size: 138 Fragments arranged by size: 2060 1712 1443 345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6096 6128 6096 Size: 32 6104 Tth111I GACn'n_nGTC Cuts at: 1316 2195 1316 Size: 879 5257 XbaI T'CTAG_A Cuts at: 3472 3472 Size: 6136 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2663 2743 3034 2663 Size: 80 291 5765 Fragments arranged by size: 5765 291 80 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6136 XmaI C'CCGG_G Cuts at: 1870 3461 1870 Size: 1591 4545 XmnI GAAnn'nnTTC Cuts at: 4265 4265 Size: 6136 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2197 3944 276 Size: 53 83 186 936 663 1747 2468 Fragments arranged by size: 2468 1747 936 663 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbvCI BfrBI BplI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI