Restriction map for
pFN21AA1144
(Linear) (Six-base) MAPSORT of: KIAA1144.seq Check: 137 from: 1 to: 1431
KIAA1144.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AatII G_ACGT'C
Cuts at: 0 194 1431
Size: 194 1237
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 549 1431
Size: 285 264 882
Fragments arranged by size:
882 285 264
AflIII A'CryG_T
Cuts at: 0 23 1431
Size: 23 1408
AhdI GACnn_n'nnGTC
Cuts at: 0 795 1431
Size: 795 636
AleI CACnn'nnGTG
Cuts at: 0 823 1431
Size: 823 608
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 661 693 1431
Size: 661 32 738
Fragments arranged by size:
738 661 32
AlwNI CAG_nnn'CTG
Cuts at: 0 15 888 1431
Size: 15 873 543
Fragments arranged by size:
873 543 15
AvaI C'yCGr_G
Cuts at: 0 115 388 1431
Size: 115 273 1043
Fragments arranged by size:
1043 273 115
AvrII C'CTAG_G
Cuts at: 0 673 1431
Size: 673 758
BanI G'GyrC_C
Cuts at: 0 1253 1431
Size: 1253 178
BanII G_rGCy'C
Cuts at: 0 173 230 389 1396 1431
Size: 173 57 159 1007 35
Fragments arranged by size:
1007 173 159 57 35
BbeI G_GCGC'C
Cuts at: 0 1257 1431
Size: 1257 174
BbsI GAAGACnn'nnnn_
Cuts at: 0 285 428 555 1431
Size: 285 143 127 876
Fragments arranged by size:
876 285 143 127
BlpI GC'TnA_GC
Cuts at: 0 281 553 1431
Size: 281 272 878
Fragments arranged by size:
878 281 272
BmgBI CAC'GTC
Cuts at: 0 24 273 434 1431
Size: 24 249 161 997
Fragments arranged by size:
997 249 161 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 330 1431
Size: 330 1101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1431
Size: 187 1244
Bpu10I CC'TnA_GC
Cuts at: 0 224 1431
Size: 224 1207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1288 1431
Size: 742 546 143
Fragments arranged by size:
742 546 143
BsaAI yAC'GTr
Cuts at: 0 240 1431
Size: 240 1191
BsaBI GATnn'nnATC
Cuts at: 0 58 901 1021 1431
Size: 58 843 120 410
Fragments arranged by size:
843 410 120 58
BsaHI Gr'CG_yC
Cuts at: 0 191 470 1254 1431
Size: 191 279 784 177
Fragments arranged by size:
784 279 191 177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 301 331 457 487 1431
Size: 301 30 126 30 944
Fragments arranged by size:
944 301 126 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 96 1072 1431
Size: 96 976 359
Fragments arranged by size:
976 359 96
BsiEI CG_ry'CG
Cuts at: 0 216 1431
Size: 216 1215
BsiHKAI G_wGCw'C
Cuts at: 0 173 230 430 695 850 1396 1431
Size: 173 57 200 265 155 546 35
Fragments arranged by size:
546 265 200 173 155 57 35
BsmI GAATG_Cn'
Cuts at: 0 777 1431
Size: 777 654
BsmBI CGTCTCn'nnnn_
Cuts at: 0 112 1431
Size: 112 1319
Bsp1286I G_dGCh'C
Cuts at: 0 173 230 389 430 695 850 1396 1431
Size: 173 57 159 41 265 155 546 35
Fragments arranged by size:
546 265 173 159 155 57 41 35
BsrBI CCG'CTC
Cuts at: 0 947 1431
Size: 947 484
BsrDI GCAATG_nn'
Cuts at: 0 701 1431
Size: 701 730
BsrFI r'CCGG_y
Cuts at: 0 4 259 1250 1431
Size: 4 255 991 181
Fragments arranged by size:
991 255 181 4
BssSI C'ACGA_G
Cuts at: 0 1198 1431
Size: 1198 233
BstAPI GCAn_nnn'nTGC
Cuts at: 0 102 1431
Size: 102 1329
BstBI TT'CG_AA
Cuts at: 0 680 1431
Size: 680 751
BstXI CCAn_nnnn'nTGG
Cuts at: 0 585 1431
Size: 585 846
BstYI r'GATC_y
Cuts at: 0 53 449 902 1431
Size: 53 396 453 529
Fragments arranged by size:
529 453 396 53
Bsu36I CC'TnA_GG
Cuts at: 0 889 1431
Size: 889 542
BtgI C'CryG_G
Cuts at: 0 369 1010 1031 1151 1431
Size: 369 641 21 120 280
Fragments arranged by size:
641 369 280 120 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 191 1431
Size: 191 1240
DraI TTT'AAA
Cuts at: 0 1415 1431
Size: 1415 16
DraIII CAC_nnn'GTG
Cuts at: 0 611 1402 1431
Size: 611 791 29
Fragments arranged by size:
791 611 29
EaeI y'GGCC_r
Cuts at: 0 6 372 728 923 1073 1431
Size: 6 366 356 195 150 358
Fragments arranged by size:
366 358 356 195 150 6
EarI CTCTTCn'nnn_
Cuts at: 0 235 1431
Size: 235 1196
EcoICRI GAG'CTC
Cuts at: 0 171 228 1394 1431
Size: 171 57 1166 37
Fragments arranged by size:
1166 171 57 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 285 549 1431
Size: 187 98 264 882
Fragments arranged by size:
882 264 187 98
EcoO109I rG'GnC_Cy
Cuts at: 0 669 885 1068 1314 1431
Size: 669 216 183 246 117
Fragments arranged by size:
669 246 216 183 117
FspI TGC'GCA
Cuts at: 0 1282 1431
Size: 1282 149
HaeII r_GCGC'y
Cuts at: 0 529 1257 1431
Size: 529 728 174
Fragments arranged by size:
728 529 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 781 813 1431
Size: 781 32 618
Fragments arranged by size:
781 618 32
HindIII A'AGCT_T
Cuts at: 0 265 1431
Size: 265 1166
KasI G'GCGC_C
Cuts at: 0 1253 1431
Size: 1253 178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 1431
Size: 8 1423
MscI TGG'CCA
Cuts at: 0 730 925 1075 1431
Size: 730 195 150 356
Fragments arranged by size:
730 356 195 150
MslI CAynn'nnrTG
Cuts at: 0 64 583 607 823 1198 1431
Size: 64 519 24 216 375 233
Fragments arranged by size:
519 375 233 216 64 24
MspA1I CmG'CkG
Cuts at: 0 198 516 1286 1431
Size: 198 318 770 145
Fragments arranged by size:
770 318 198 145
NarI GG'CG_CC
Cuts at: 0 1254 1431
Size: 1254 177
NcoI C'CATG_G
Cuts at: 0 369 1431
Size: 369 1062
PasI CC'CwG_GG
Cuts at: 0 951 1144 1431
Size: 951 193 287
Fragments arranged by size:
951 287 193
PpuMI rG'GwC_Cy
Cuts at: 0 669 885 1314 1431
Size: 669 216 429 117
Fragments arranged by size:
669 429 216 117
PstI C_TGCA'G
Cuts at: 0 358 1431
Size: 358 1073
PvuII CAG'CTG
Cuts at: 0 516 1286 1431
Size: 516 770 145
Fragments arranged by size:
770 516 145
SacI G_AGCT'C
Cuts at: 0 173 230 1396 1431
Size: 173 57 1166 35
Fragments arranged by size:
1166 173 57 35
SapI GCTCTTCn'nnn_
Cuts at: 0 235 1431
Size: 235 1196
ScaI AGT'ACT
Cuts at: 0 319 1431
Size: 319 1112
SexAI A'CCwGG_T
Cuts at: 0 833 1431
Size: 833 598
SfcI C'TryA_G
Cuts at: 0 354 360 1431
Size: 354 6 1071
Fragments arranged by size:
1071 354 6
SfoI GGC'GCC
Cuts at: 0 1255 1431
Size: 1255 176
SmlI C'TyrA_G
Cuts at: 0 757 1267 1431
Size: 757 510 164
Fragments arranged by size:
757 510 164
StyI C'CwwG_G
Cuts at: 0 369 673 1431
Size: 369 304 758
Fragments arranged by size:
758 369 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1311 1431
Size: 1311 120
TatI w'GTAC_w
Cuts at: 0 317 1431
Size: 317 1114
Tth111I GACn'n_nGTC
Cuts at: 0 190 1431
Size: 190 1241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 658 738 1029 1431
Size: 658 80 291 402
Fragments arranged by size:
658 402 291 80
ZraI GAC'GTC
Cuts at: 0 192 1431
Size: 192 1239
Enzymes that do cut:
AatII AcuI AflIII AhdI AleI AloI AlwNI AvaI
AvrII BanI BanII BbeI BbsI BlpI BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
DraI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FspI
HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I
NarI NcoI PasI PpuMI PstI PvuII SacI SapI
ScaI SexAI SfcI SfoI SmlI StyI TaqII TatI
Tth111I XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AlfI
ApaI ApaLI ApoI AscI AseI AsiSI BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I
BmtI BplI BsaI BsaWI BseYI BsgI BsiWI BspEI
BspHI BspMI BsrGI BssHII BstEII BstZ17I BtsI ClaI
CspCI DrdI EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspAI HincII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI XbaI XhoI XmaI
XmnI