(Circular) (Six-base) MAPSORT of: pFN21AA1140.seq Check: 6037 from: 1 to: 6922 pFN21AA1140 6922 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3366 3469 3366 Size: 103 6819 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4732 278 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4265 1937 Size: 2328 4594 Acc65I G'GTAC_C Cuts at: 2898 4243 2898 Size: 1345 5577 AclI AA'CG_TT Cuts at: 1563 5049 5422 1563 Size: 3486 373 3063 Fragments arranged by size: 3486 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2423 3185 3344 3764 4985 6369 6637 1632 Size: 791 762 159 420 1221 1384 268 1917 Fragments arranged by size: 1917 1384 1221 791 762 420 268 159 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6725 AflIII A'CryG_T Cuts at: 1203 2042 5821 6798 1203 Size: 839 3779 977 1327 Fragments arranged by size: 3779 1327 977 839 AgeI A'CCGG_T Cuts at: 5753 5753 Size: 6922 AhdI GACnn_n'nnGTC Cuts at: 2834 5651 2834 Size: 2817 4105 AleI CACnn'nnGTG Cuts at: 1166 3464 1166 Size: 2298 4624 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3539 3859 1184 Size: 2355 320 4247 Fragments arranged by size: 4247 2355 320 AlwNI CAG_nnn'CTG Cuts at: 2617 3208 3226 3484 3544 3662 4102 4665 6237 Size: 591 18 258 60 118 440 563 1572 Cuts at: 6237 2617 Size: 3302 Fragments arranged by size: 3302 1572 591 563 440 258 118 60 18 ApaI G_GGCC'C Cuts at: 3755 3755 Size: 6922 ApaLI G'TGCA_C Cuts at: 4979 6135 4979 Size: 1156 5766 ApoI r'AATT_y Cuts at: 1493 2885 4231 4457 4609 6718 1493 Size: 1392 1346 226 152 2109 1697 Fragments arranged by size: 2109 1697 1392 1346 226 152 AseI AT'TA_AT Cuts at: 160 1032 5476 160 Size: 872 4444 1606 Fragments arranged by size: 4444 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 AvaI C'yCGr_G Cuts at: 1870 1943 2718 4247 1870 Size: 73 775 1529 4545 Fragments arranged by size: 4545 1529 775 73 BamHI G'GATC_C Cuts at: 4252 4619 4252 Size: 367 6555 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2740 2898 3229 4243 5698 Size: 325 217 627 953 158 331 1014 1455 Cuts at: 5698 618 Size: 1842 Fragments arranged by size: 1842 1455 1014 953 627 331 325 217 158 BanII G_rGCy'C Cuts at: 1391 2355 2439 2555 3220 3755 4221 4241 1391 Size: 964 84 116 665 535 466 20 4072 Fragments arranged by size: 4072 964 665 535 466 116 84 20 BbeI G_GCGC'C Cuts at: 3233 3233 Size: 6922 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2300 928 Size: 971 401 5550 Fragments arranged by size: 5550 971 401 BbvCI CC'TCA_GC Cuts at: 3527 3912 3527 Size: 385 6537 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5113 5147 1903 Size: 34 50 34 3092 34 3678 Fragments arranged by size: 3678 3092 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4814 6024 4814 Size: 1210 5712 BclI T'GATC_A Cuts at: 1548 2235 3072 1548 Size: 687 837 5398 Fragments arranged by size: 5398 837 687 BfrBI ATG'CAT Cuts at: 3994 3994 Size: 6922 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2612 5533 136 Size: 107 122 71 631 605 940 2921 1525 Fragments arranged by size: 2921 1525 940 631 605 122 107 71 BglII A'GATC_T Cuts at: 3288 3783 6917 3288 Size: 495 3134 3293 Fragments arranged by size: 3293 3134 495 BlpI GC'TnA_GC Cuts at: 2732 3219 3801 4339 2732 Size: 487 582 538 5315 Fragments arranged by size: 5315 582 538 487 Bme1580I G_kGCm'C Cuts at: 1790 3000 3755 4983 6139 1790 Size: 1210 755 1228 1156 2573 Fragments arranged by size: 2573 1228 1210 1156 755 BmgBI CAC'GTC Cuts at: 1318 2313 1318 Size: 995 5927 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3228 4653 5611 Size: 416 53 83 399 24 1811 1425 958 Cuts at: 5611 442 Size: 1753 Fragments arranged by size: 1811 1753 1425 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6922 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2272 2666 2272 Size: 394 6528 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 2569 2876 3230 3277 3494 3722 3950 Size: 1124 206 307 354 47 217 228 228 Cuts at: 3950 4193 5582 1239 Size: 243 1389 2579 Fragments arranged by size: 2579 1389 1124 354 307 243 228 228 217 206 47 Bpu10I CC'TnA_GC Cuts at: 2268 2804 3527 3912 2268 Size: 536 723 385 5278 Fragments arranged by size: 5278 723 536 385 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3812 4187 5047 5912 6210 3812 Size: 375 860 865 298 4524 Fragments arranged by size: 4524 865 860 375 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2456 3134 5585 882 Size: 617 957 678 2451 2219 Fragments arranged by size: 2451 2219 957 678 617 BsaAI yAC'GTr Cuts at: 493 1204 4080 4713 493 Size: 711 2876 633 2702 Fragments arranged by size: 2876 2702 711 633 BsaBI GATnn'nnATC Cuts at: 3071 6916 3071 Size: 3845 3077 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3156 3230 4729 5111 Size: 53 83 186 936 1623 74 1499 382 Cuts at: 5111 5746 275 Size: 635 1451 Fragments arranged by size: 1623 1499 1451 936 635 382 186 83 74 53 BsaWI w'CCGG_w Cuts at: 2760 3189 5355 5753 6027 6174 6585 2760 Size: 429 2166 398 274 147 411 3097 Fragments arranged by size: 3097 2166 429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3539 3569 3650 3680 3698 Size: 30 165 30 2136 30 81 30 18 Cuts at: 3698 3728 1178 Size: 30 4372 Fragments arranged by size: 4372 2136 165 81 30 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 2552 2606 2681 2684 2768 3719 3947 Size: 1054 312 54 75 3 84 951 228 Cuts at: 3947 1186 Size: 4161 Fragments arranged by size: 4161 1054 951 312 228 84 75 54 3 BseYI C'CCAG_C Cuts at: 3558 3562 4021 6125 6621 3558 Size: 4 459 2104 496 3859 Fragments arranged by size: 3859 2104 496 459 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2507 1163 Size: 573 771 5578 Fragments arranged by size: 5578 771 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4372 5133 6161 1540 Size: 275 186 2371 761 1028 2301 Fragments arranged by size: 2371 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2355 2439 3057 3435 4221 4983 5068 6139 2355 Size: 84 618 378 786 762 85 1071 3138 Fragments arranged by size: 3138 1071 786 762 618 378 85 84 BsmI GAATG_Cn' Cuts at: 2785 4433 4526 2785 Size: 1648 93 5181 Fragments arranged by size: 5181 1648 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2355 2439 2555 3000 3057 3220 3435 Size: 399 565 84 116 445 57 163 215 Cuts at: 3435 3755 4221 4241 4983 5068 6139 1391 Size: 320 466 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 565 466 445 399 320 215 163 116 85 84 57 20 BspEI T'CCGG_A Cuts at: 6585 6585 Size: 6922 BspHI T'CATG_A Cuts at: 4811 6541 4811 Size: 1730 5192 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3366 3469 3521 3827 4277 844 Size: 2522 103 52 306 450 3489 Fragments arranged by size: 3489 2522 450 306 103 52 BsrBI CCG'CTC Cuts at: 1394 1823 4809 1394 Size: 429 2986 3507 Fragments arranged by size: 3507 2986 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5417 5591 65 Size: 1183 4169 174 1396 Fragments arranged by size: 4169 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3903 5566 5753 1700 Size: 110 2093 1663 187 2869 Fragments arranged by size: 2869 2093 1663 187 110 BsrGI T'GTAC_A Cuts at: 96 3546 3948 96 Size: 3450 402 3070 Fragments arranged by size: 3450 3070 402 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6922 BssSI C'ACGA_G Cuts at: 3506 4085 4982 5994 3506 Size: 579 897 1012 4434 Fragments arranged by size: 4434 1012 897 579 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6922 BstBI TT'CG_AA Cuts at: 5726 5726 Size: 6922 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6922 BstXI CCAn_nnnn'nTGG Cuts at: 2506 3301 2506 Size: 795 6127 BstYI r'GATC_y Cuts at: 1972 3288 3783 4252 4619 5004 5021 6473 6559 Size: 1316 495 469 367 385 17 1452 86 Cuts at: 6559 6571 6917 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1316 495 469 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2048 2709 2916 3300 4062 513 Size: 552 983 661 207 384 762 3373 Fragments arranged by size: 3373 983 762 661 552 384 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2179 3670 487 Size: 683 56 953 1491 3739 Fragments arranged by size: 3739 1491 953 683 56 BtsI GCAGTG_nn' Cuts at: 3197 3365 4438 5232 5252 3197 Size: 168 1073 794 20 4867 Fragments arranged by size: 4867 1073 794 168 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6922 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6887 DraI TTT'AAA Cuts at: 4226 4226 Size: 6922 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3245 5929 809 Size: 507 1929 2684 1802 Fragments arranged by size: 2684 1929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2496 3006 3420 Size: 54 1425 50 275 18 666 510 414 Cuts at: 3420 4369 5258 6887 8 Size: 949 889 1629 43 Fragments arranged by size: 1629 1425 949 889 666 510 414 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4369 1537 Size: 275 2557 4090 Fragments arranged by size: 4090 2557 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3377 3739 3872 4852 1353 Size: 2024 362 133 980 3423 Fragments arranged by size: 3423 2024 980 362 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2198 5509 5883 6029 387 Size: 1064 138 609 3311 374 146 1280 Fragments arranged by size: 3311 1280 1064 609 374 146 138 EcoICRI GAG'CTC Cuts at: 2353 2437 4219 2353 Size: 84 1782 5056 Fragments arranged by size: 5056 1782 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2363 2423 2569 2876 3185 3230 3277 Size: 393 731 60 146 307 309 45 47 Cuts at: 3277 3344 3494 3722 3764 3950 4193 4985 5582 Size: 67 150 228 42 186 243 792 597 Cuts at: 5582 6369 6637 1239 Size: 787 268 1524 Fragments arranged by size: 1524 792 787 731 597 393 309 307 268 243 228 186 150 146 67 60 47 45 42 EcoNI CCTnn'n_nnAGG Cuts at: 2405 2534 3083 3107 3575 3746 3932 4106 2405 Size: 129 549 24 468 171 186 174 5221 Fragments arranged by size: 5221 549 468 186 174 171 129 24 EcoO109I rG'GnC_Cy Cuts at: 2151 2253 2515 2614 3027 3319 3598 3751 3752 Size: 102 262 99 413 292 279 153 1 Cuts at: 3752 4099 2151 Size: 347 4974 Fragments arranged by size: 4974 413 347 292 279 262 153 102 99 1 EcoRI G'AATT_C Cuts at: 4231 4609 4231 Size: 378 6544 FspI TGC'GCA Cuts at: 3986 5428 3986 Size: 1442 5480 FspAI rTGC'GCAy Cuts at: 3986 3986 Size: 6922 HaeII r_GCGC'y Cuts at: 1678 2388 3233 3962 6069 1678 Size: 710 845 729 2107 2531 Fragments arranged by size: 2531 2107 845 729 710 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2029 2061 2820 2852 3644 Size: 32 135 32 457 32 759 32 792 Cuts at: 3644 3650 3676 3682 3698 3730 4000 4032 5563 Size: 6 26 6 16 32 270 32 1531 Cuts at: 5563 5595 5637 5669 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1531 792 759 457 270 135 42 32 32 32 32 32 32 32 32 26 16 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3415 4147 4266 4512 669 Size: 988 281 1477 732 119 246 3079 Fragments arranged by size: 3079 1477 988 732 281 246 119 HindIII A'AGCT_T Cuts at: 748 748 Size: 6922 HpaI GTT'AAC Cuts at: 4512 4512 Size: 6922 KasI G'GCGC_C Cuts at: 3229 3229 Size: 6922 KpnI G_GTAC'C Cuts at: 2902 4247 2902 Size: 1345 5577 MfeI C'AATT_G Cuts at: 4521 4521 Size: 6922 MluI A'CGCG_T Cuts at: 6798 6798 Size: 6922 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2066 3307 6036 6220 1305 Size: 761 1241 2729 184 2007 Fragments arranged by size: 2729 2007 1241 761 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3422 10 Size: 54 1425 343 1590 3510 Fragments arranged by size: 3510 1590 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2014 2047 2634 3068 3396 3464 4880 Size: 648 848 33 587 434 328 68 1416 Cuts at: 4880 5239 5398 518 Size: 359 159 2042 Fragments arranged by size: 2042 1416 848 648 587 434 359 328 159 68 33 MspA1I CmG'CkG Cuts at: 1606 3226 3271 3310 3562 3763 3946 4338 5015 Size: 1620 45 39 252 201 183 392 677 Cuts at: 5015 6163 6408 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1620 1148 677 392 252 245 201 183 45 39 NaeI GCC'GGC Cuts at: 3905 3905 Size: 6922 NarI GG'CG_CC Cuts at: 3230 3230 Size: 6922 NcoI C'CATG_G Cuts at: 513 1065 2048 2709 2916 3300 513 Size: 552 983 661 207 384 4135 Fragments arranged by size: 4135 983 661 552 384 207 NdeI CA'TA_TG Cuts at: 387 387 Size: 6922 NgoMIV G'CCGG_C Cuts at: 3903 3903 Size: 6922 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6922 NotI GC'GGCC_GC Cuts at: 4369 4369 Size: 6922 NsiI A_TGCA'T Cuts at: 3996 3996 Size: 6922 NspI r_CATG'y Cuts at: 1132 1229 2019 2046 2111 3401 3628 4280 5825 Size: 97 790 27 65 1290 227 652 1545 Cuts at: 5825 1132 Size: 2229 Fragments arranged by size: 2229 1545 1290 790 652 227 97 65 27 PasI CC'CwG_GG Cuts at: 1348 4123 1348 Size: 2775 4147 PciI A'CATG_T Cuts at: 2042 5821 2042 Size: 3779 3143 PflMI CCAn_nnn'nTGG Cuts at: 1108 3361 1108 Size: 2253 4669 PfoI T'CCnGG_A Cuts at: 2258 2364 2439 3849 2258 Size: 106 75 1410 5331 Fragments arranged by size: 5331 1410 106 75 PmeI GTTT'AAAC Cuts at: 4226 4226 Size: 6922 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4991 5023 6504 6536 4991 Size: 32 1481 32 5377 Fragments arranged by size: 5377 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2151 2515 3027 2151 Size: 364 512 6046 Fragments arranged by size: 6046 512 364 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6922 PsiI TTA'TAA Cuts at: 4492 4492 Size: 6922 PspOMI G'GGCC_C Cuts at: 3751 3751 Size: 6922 PstI C_TGCA'G Cuts at: 830 2159 2283 3147 3204 3270 3315 3518 3795 Size: 1329 124 864 57 66 45 203 277 Cuts at: 3795 4119 4274 830 Size: 324 155 3478 Fragments arranged by size: 3478 1329 864 324 277 203 155 124 66 57 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6922 PvuII CAG'CTG Cuts at: 3226 3271 3310 3562 3763 3946 3226 Size: 45 39 252 201 183 6202 Fragments arranged by size: 6202 252 201 183 45 39 SacI G_AGCT'C Cuts at: 2355 2439 4221 2355 Size: 84 1782 5056 Fragments arranged by size: 5056 1782 84 SalI G'TCGA_C Cuts at: 1936 4264 1936 Size: 2328 4594 SanDI GG'GwC_CC Cuts at: 3027 3027 Size: 6922 SapI GCTCTTCn'nnn_ Cuts at: 3377 3377 Size: 6922 SbfI CC_TGCA'GG Cuts at: 2159 2283 3147 4274 2159 Size: 124 864 1127 4807 Fragments arranged by size: 4807 1127 864 124 ScaI AGT'ACT Cuts at: 3888 5170 6882 3888 Size: 1282 1712 3928 Fragments arranged by size: 3928 1712 1282 SexAI A'CCwGG_T Cuts at: 2901 2901 Size: 6922 SfcI C'TryA_G Cuts at: 826 1045 2155 2279 3136 3143 3200 3266 3311 Size: 219 1110 124 857 7 57 66 45 Cuts at: 3311 3514 3791 4115 4270 5405 6086 6277 826 Size: 203 277 324 155 1135 681 191 1471 Fragments arranged by size: 1471 1135 1110 857 681 324 277 219 203 191 155 124 66 57 45 7 SfoI GGC'GCC Cuts at: 3231 3231 Size: 6922 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6922 SmaI CCC'GGG Cuts at: 1872 4249 1872 Size: 2377 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2718 3827 4166 5026 5927 6189 Size: 197 926 775 1109 339 860 901 262 Cuts at: 6189 820 Size: 1553 Fragments arranged by size: 1553 1109 926 901 860 775 339 262 197 SnaBI TAC'GTA Cuts at: 493 4713 493 Size: 4220 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6922 SphI G_CATG'C Cuts at: 1132 2111 3401 3628 4280 1132 Size: 979 1290 227 652 3774 Fragments arranged by size: 3774 1290 979 652 227 SspI AAT'ATT Cuts at: 5 52 4846 5 Size: 47 4794 2081 Fragments arranged by size: 4794 2081 47 StuI AGG'CCT Cuts at: 1517 2166 3570 1517 Size: 649 1404 4869 Fragments arranged by size: 4869 1404 649 StyI C'CwwG_G Cuts at: 513 1065 2048 2661 2709 2916 3108 3300 3462 Size: 552 983 613 48 207 192 192 162 Cuts at: 3462 4361 513 Size: 899 3074 Fragments arranged by size: 3074 983 899 613 552 207 192 192 162 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3727 4949 4966 5119 5304 5749 325 Size: 1255 2147 1222 17 153 185 445 1498 Fragments arranged by size: 2147 1498 1255 1222 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3546 3886 3948 Size: 274 80 33 51 1443 1569 340 62 Cuts at: 3948 4189 5168 6880 96 Size: 241 979 1712 138 Fragments arranged by size: 1712 1569 1443 979 340 274 241 138 80 62 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6882 6914 6882 Size: 32 6890 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6922 XbaI T'CTAG_A Cuts at: 4258 4258 Size: 6922 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3115 3469 3115 Size: 354 6568 XhoI C'TCGA_G Cuts at: 1943 2718 1943 Size: 775 6147 XmaI C'CCGG_G Cuts at: 1870 4247 1870 Size: 2377 4545 XmnI GAAnn'nnTTC Cuts at: 5051 5051 Size: 6922 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4730 276 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI NruI PacI PmlI PspXI PsrI RsrII SacII SfiI SrfI SwaI