Restriction map for pFN21AA1132
##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

AatII       AclI        AflII       AhdI        AocI        
AscI        AseI        AsnI        AspEI       AvrII       
BamHI       BciVI       BfrI        BlnI        BsaBI       
BsmI        BspDI       BssHII      Bst1107I    BstZ17I     
Bsu36I      ClaI        DraI        Eam1105I    Eco81I      
EcoRV       HpaI        MamI        MfeI        MluI        
MunI        NdeI        NotI        NruI        PacI        
PmeI        PshAI       PshBI       Psp1406I    PvuI        
SalI        SexAI       SfiI        SgrAI       SnaBI       
SpeI        SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

Acc65I        176  GGTACC               G^GTACC
AccI         3148  GTCTAC               GT^[AC][GT]AC
Asp700I      4770  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       176  GGTACC               G^GTACC
AviII        4646  TGCGCA               TGC^GCA
BbeI         1297  GGCGCC               GGCGC^C
BglI         6004  GCCCCTCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1964  AGATCT               A^GATCT
BspHI        1949  TCATGA               T^CATGA
BspLU11I     5153  ACATGT               A^CATGT
BssSI        6032  CACGAG               CACGAG(-5/-1)
BstAPI       3777  GCAGTATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoT22I      2004  ATGCAT               ATGCA^T
FspI         4646  TGCGCA               TGC^GCA
HindIII      4920  AAGCTT               A^AGCTT
KasI         1297  GGCGCC               G^GCGCC
KpnI          176  GGTACC               GGTAC^C
MseI         4493  TTAA                 T^TAA
NarI         1297  GGCGCC               GG^CGCC
NheI         3610  GCTAGC               G^CTAGC
NsiI         2004  ATGCAT               ATGCA^T
PaeR7I       6082  CTCGAG               C^TCGAG
RcaI         1949  TCATGA               T^CATGA
SbfI         4497  CCTGCAGG             CCTGCA^GG
ScaI         4256  AGTACT               AGT^ACT
SfoI         1297  GGCGCC               GGC^GCC
Sse8387I     4497  CCTGCAGG             CCTGCA^GG
Tru9I        4493  TTAA                 T^TAA
XhoI         6082  CTCGAG               C^TCGAG
XmnI         4770  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AccIII        335  TCCGGA               T^CCGGA
             5789  TCCGGA              
AcsI         1807  GAATTC               [GA]^AATT[CT]
             1945  GAATTC              
AflIII       4082  ACACGT               A^C[GA][CT]GT
             5153  ACATGT              
AgeI         1448  ACCGGT               A^CCGGT
             5225  ACCGGT              
Alw44I       1452  GTGCAC               G^TGCAC
             4086  GTGCAC              
Aor51HI      4310  AGCGCT               AGC^GCT
             5998  AGCGCT              
ApaLI        1452  GTGCAC               G^TGCAC
             4086  GTGCAC              
ApoI         1807  GAATTC               [GA]^AATT[CT]
             1945  GAATTC              
BbvCI        4181  CCTCAGC              CCTCAGC(-5/-2)
             4063  GCTGAGG             
BcgI         3237  CGAGATCAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1577  GCAGGGTCATCG        
BclI         1907  TGATCA               T^GATCA
             2369  TGATCA              
BlpI         1095  GCTCAGC              GC^T[ACGT]AGC
             5977  GCTGAGC             
Bpu1102I     1095  GCTCAGC              GC^T[ACGT]AGC
             5977  GCTGAGC             
BsaI          619  GAGACC               GGTCTC(1/5)
             6110  GGTCTC              
BseAI         335  TCCGGA               T^CCGGA
             5789  TCCGGA              
BsiEI         143  CGGCCG               CG[GA][CT]^CG
             3813  CGGCCG              
BsiWI        3528  CGTACG               C^GTACG
             4833  CGTACG              
Bsp1407I      545  TGTACA               T^GTACA
             2870  TGTACA              
BspEI         335  TCCGGA               T^CCGGA
             5789  TCCGGA              
BsrGI         545  TGTACA               T^GTACA
             2870  TGTACA              
BstBI        1804  TTCGAA               TT^CGAA
             5732  TTCGAA              
CelII        1095  GCTCAGC              GC^T[ACGT]AGC
             5977  GCTGAGC             
CpoI          853  CGGACCG              CG^G[AT]CCG
             5636  CGGACCG             
EagI          143  CGGCCG               C^GGCCG
             3813  CGGCCG              
EclXI         143  CGGCCG               C^GGCCG
             3813  CGGCCG              
Eco47III     4310  AGCGCT               AGC^GCT
             5998  AGCGCT              
Eco52I        143  CGGCCG               C^GGCCG
             3813  CGGCCG              
EcoRI        1807  GAATTC               G^AATTC
             1945  GAATTC              
FbaI         1907  TGATCA               T^GATCA
             2369  TGATCA              
FseI          714  GGCCGGCC             GGCCGG^CC
              787  GGCCGGCC            
MroI          335  TCCGGA               T^CCGGA
             5789  TCCGGA              
NcoI         2969  CCATGG               C^CATGG
             4004  CCATGG              
NspV         1804  TTCGAA               TT^CGAA
             5732  TTCGAA              
PinAI        1448  ACCGGT               A^CCGGT
             5225  ACCGGT              
RsrII         853  CGGACCG              CG^G[AT]CCG
             5636  CGGACCG             
SacI         1953  GAGCTC               GAGCT^C
             2926  GAGCTC              
SfuI         1804  TTCGAA               TT^CGAA
             5732  TTCGAA              
SspBI         545  TGTACA               T^GTACA
             2870  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  141 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AspI        
AvaI        AvaII       BalI        BanI        BanII       
BbrPI       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaAI       BsaHI       
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DrdI        DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflFI       PflMI       PleI        
PmaCI       PmlI        PpuMI       PstI        PvuII       
RsaI        SacII       SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
SphI        StuI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI        
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