Restriction map for
pFN21AA1132
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# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AclI AflII AhdI AocI
AscI AseI AsnI AspEI AvrII
BamHI BciVI BfrI BlnI BsaBI
BsmI BspDI BssHII Bst1107I BstZ17I
Bsu36I ClaI DraI Eam1105I Eco81I
EcoRV HpaI MamI MfeI MluI
MunI NdeI NotI NruI PacI
PmeI PshAI PshBI Psp1406I PvuI
SalI SexAI SfiI SgrAI SnaBI
SpeI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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Acc65I 176 GGTACC G^GTACC
AccI 3148 GTCTAC GT^[AC][GT]AC
Asp700I 4770 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 176 GGTACC G^GTACC
AviII 4646 TGCGCA TGC^GCA
BbeI 1297 GGCGCC GGCGC^C
BglI 6004 GCCCCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1964 AGATCT A^GATCT
BspHI 1949 TCATGA T^CATGA
BspLU11I 5153 ACATGT A^CATGT
BssSI 6032 CACGAG CACGAG(-5/-1)
BstAPI 3777 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoT22I 2004 ATGCAT ATGCA^T
FspI 4646 TGCGCA TGC^GCA
HindIII 4920 AAGCTT A^AGCTT
KasI 1297 GGCGCC G^GCGCC
KpnI 176 GGTACC GGTAC^C
MseI 4493 TTAA T^TAA
NarI 1297 GGCGCC GG^CGCC
NheI 3610 GCTAGC G^CTAGC
NsiI 2004 ATGCAT ATGCA^T
PaeR7I 6082 CTCGAG C^TCGAG
RcaI 1949 TCATGA T^CATGA
SbfI 4497 CCTGCAGG CCTGCA^GG
ScaI 4256 AGTACT AGT^ACT
SfoI 1297 GGCGCC GGC^GCC
Sse8387I 4497 CCTGCAGG CCTGCA^GG
Tru9I 4493 TTAA T^TAA
XhoI 6082 CTCGAG C^TCGAG
XmnI 4770 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 38 enzymes) #
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AccIII 335 TCCGGA T^CCGGA
5789 TCCGGA
AcsI 1807 GAATTC [GA]^AATT[CT]
1945 GAATTC
AflIII 4082 ACACGT A^C[GA][CT]GT
5153 ACATGT
AgeI 1448 ACCGGT A^CCGGT
5225 ACCGGT
Alw44I 1452 GTGCAC G^TGCAC
4086 GTGCAC
Aor51HI 4310 AGCGCT AGC^GCT
5998 AGCGCT
ApaLI 1452 GTGCAC G^TGCAC
4086 GTGCAC
ApoI 1807 GAATTC [GA]^AATT[CT]
1945 GAATTC
BbvCI 4181 CCTCAGC CCTCAGC(-5/-2)
4063 GCTGAGG
BcgI 3237 CGAGATCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1577 GCAGGGTCATCG
BclI 1907 TGATCA T^GATCA
2369 TGATCA
BlpI 1095 GCTCAGC GC^T[ACGT]AGC
5977 GCTGAGC
Bpu1102I 1095 GCTCAGC GC^T[ACGT]AGC
5977 GCTGAGC
BsaI 619 GAGACC GGTCTC(1/5)
6110 GGTCTC
BseAI 335 TCCGGA T^CCGGA
5789 TCCGGA
BsiEI 143 CGGCCG CG[GA][CT]^CG
3813 CGGCCG
BsiWI 3528 CGTACG C^GTACG
4833 CGTACG
Bsp1407I 545 TGTACA T^GTACA
2870 TGTACA
BspEI 335 TCCGGA T^CCGGA
5789 TCCGGA
BsrGI 545 TGTACA T^GTACA
2870 TGTACA
BstBI 1804 TTCGAA TT^CGAA
5732 TTCGAA
CelII 1095 GCTCAGC GC^T[ACGT]AGC
5977 GCTGAGC
CpoI 853 CGGACCG CG^G[AT]CCG
5636 CGGACCG
EagI 143 CGGCCG C^GGCCG
3813 CGGCCG
EclXI 143 CGGCCG C^GGCCG
3813 CGGCCG
Eco47III 4310 AGCGCT AGC^GCT
5998 AGCGCT
Eco52I 143 CGGCCG C^GGCCG
3813 CGGCCG
EcoRI 1807 GAATTC G^AATTC
1945 GAATTC
FbaI 1907 TGATCA T^GATCA
2369 TGATCA
FseI 714 GGCCGGCC GGCCGG^CC
787 GGCCGGCC
MroI 335 TCCGGA T^CCGGA
5789 TCCGGA
NcoI 2969 CCATGG C^CATGG
4004 CCATGG
NspV 1804 TTCGAA TT^CGAA
5732 TTCGAA
PinAI 1448 ACCGGT A^CCGGT
5225 ACCGGT
RsrII 853 CGGACCG CG^G[AT]CCG
5636 CGGACCG
SacI 1953 GAGCTC GAGCT^C
2926 GAGCTC
SfuI 1804 TTCGAA TT^CGAA
5732 TTCGAA
SspBI 545 TGTACA T^GTACA
2870 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AspI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI