Restriction map for
pFN21AA1132
(Linear) (Six-base) MAPSORT of: KIAA1132.seq Check: 4157 from: 1 to: 6159
KIAA1132.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 90 5280 6159
Size: 90 5190 879
Fragments arranged by size:
5190 879 90
AccI GT'mk_AC
Cuts at: 0 3149 6159
Size: 3149 3010
Acc65I G'GTAC_C
Cuts at: 0 176 6159
Size: 176 5983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 434 991 1224 2575 2577 4425 4882
Size: 74 360 557 233 1351 2 1848 457
Cuts at: 4882 6159
Size: 1277
Fragments arranged by size:
1848 1351 1277 557 457 360 233 74
2
AfeI AGC'GCT
Cuts at: 0 4312 6000 6159
Size: 4312 1688 159
Fragments arranged by size:
4312 1688 159
AflIII A'CryG_T
Cuts at: 0 4082 5153 6159
Size: 4082 1071 1006
Fragments arranged by size:
4082 1071 1006
AgeI A'CCGG_T
Cuts at: 0 1448 5225 6159
Size: 1448 3777 934
Fragments arranged by size:
3777 1448 934
AleI CACnn'nnGTG
Cuts at: 0 1281 4933 6154 6159
Size: 1281 3652 1221 5
Fragments arranged by size:
3652 1281 1221 5
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2728 3556 4060 6159
Size: 2728 828 504 2099
Fragments arranged by size:
2728 2099 828 504
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2572 2604 6159
Size: 2572 32 3555
Fragments arranged by size:
3555 2572 32
AlwNI CAG_nnn'CTG
Cuts at: 0 661 812 2238 3501 3783 3955 5059 5590
Size: 661 151 1426 1263 282 172 1104 531
Cuts at: 5590 6159
Size: 569
Fragments arranged by size:
1426 1263 1104 661 569 531 282 172
151
ApaI G_GGCC'C
Cuts at: 0 1326 2966 3227 3297 4957 5880 6046 6159
Size: 1326 1640 261 70 1660 923 166 113
Fragments arranged by size:
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ApaLI G'TGCA_C
Cuts at: 0 1452 4086 6159
Size: 1452 2634 2073
Fragments arranged by size:
2634 2073 1452
ApoI r'AATT_y
Cuts at: 0 1807 1945 6159
Size: 1807 138 4214
Fragments arranged by size:
4214 1807 138
AvaI C'yCGr_G
Cuts at: 0 747 1261 1860 1920 3691 5435 6082 6159
Size: 747 514 599 60 1771 1744 647 77
Fragments arranged by size:
1771 1744 747 647 599 514 77 60
BanI G'GyrC_C
Cuts at: 0 64 132 176 868 1297 1390 2320 3024
Size: 64 68 44 692 429 93 930 704
Cuts at: 3024 3889 4013 5539 6159
Size: 865 124 1526 620
Fragments arranged by size:
1526 930 865 704 692 620 429 124
93 68 64 44
BanII G_rGCy'C
Cuts at: 0 154 343 1021 1097 1326 1340 1957 2660
Size: 154 189 678 76 229 14 617 703
Cuts at: 2660 2930 2966 3227 3297 3341 4387 4436 4957
Size: 270 36 261 70 44 1046 49 521
Cuts at: 4957 5311 5756 5880 6046 6067 6159
Size: 354 445 124 166 21 92
Fragments arranged by size:
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261 229 189 166 154 124 92 76
70 49 44 36 21 14
BbeI G_GCGC'C
Cuts at: 0 1301 6159
Size: 1301 4858
BbsI GAAGACnn'nnnn_
Cuts at: 0 420 979 3989 6159
Size: 420 559 3010 2170
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 4064 4182 6159
Size: 4064 118 1977
Fragments arranged by size:
4064 1977 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1566 1600 3226 3260 6159
Size: 1566 34 1626 34 2899
Fragments arranged by size:
2899 1626 1566 34 34
BclI T'GATC_A
Cuts at: 0 1907 2369 6159
Size: 1907 462 3790
Fragments arranged by size:
3790 1907 462
BfrBI ATG'CAT
Cuts at: 0 2006 6159
Size: 2006 4153
BglI GCCn_nnn'nGGC
Cuts at: 0 6010 6159
Size: 6010 149
BglII A'GATC_T
Cuts at: 0 1964 6159
Size: 1964 4195
BlpI GC'TnA_GC
Cuts at: 0 1096 5978 6159
Size: 1096 4882 181
Fragments arranged by size:
4882 1096 181
Bme1580I G_kGCm'C
Cuts at: 0 137 642 1011 1326 1456 1787 2066 2216
Size: 137 505 369 315 130 331 279 150
Cuts at: 2216 2966 3227 3269 3297 3892 4016 4090 4957
Size: 750 261 42 28 595 124 74 867
Cuts at: 4957 5663 5880 6046 6159
Size: 706 217 166 113
Fragments arranged by size:
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279 261 217 166 150 137 130 124
113 74 42 28
BmgBI CAC'GTC
Cuts at: 0 207 225 1689 2268 3315 5749 6159
Size: 207 18 1464 579 1047 2434 410
Fragments arranged by size:
2434 1464 1047 579 410 207 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 3174 4686 4819 5032 6159
Size: 3174 1512 133 213 1127
Fragments arranged by size:
3174 1512 1127 213 133
BmtI G_CTAG'C
Cuts at: 0 3614 6159
Size: 3614 2545
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1098 1290 2562 5427 6159
Size: 1098 192 1272 2865 732
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1908 2698 3277 4287 4564 5479 6051 6159
Size: 1908 790 579 1010 277 915 572 108
Fragments arranged by size:
1908 1010 915 790 579 572 277 108
Bpu10I CC'TnA_GC
Cuts at: 0 4064 4182 6159
Size: 4064 118 1977
Fragments arranged by size:
4064 1977 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 1228 1660 1966 2545 3151 4318 4730
Size: 763 465 432 306 579 606 1167 412
Cuts at: 4730 5217 6159
Size: 487 942
Fragments arranged by size:
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412 306
BsaI GGTCTCn'nnnn_
Cuts at: 0 613 6116 6159
Size: 613 5503 43
Fragments arranged by size:
5503 613 43
BsaAI yAC'GTr
Cuts at: 0 1770 4085 4346 4634 6159
Size: 1770 2315 261 288 1525
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 572 1298 3362 5102 6159
Size: 572 726 2064 1740 1057
Fragments arranged by size:
2064 1740 1057 726 572
BsaWI w'CCGG_w
Cuts at: 0 335 1448 3749 5225 5789 6159
Size: 335 1113 2301 1476 564 370
Fragments arranged by size:
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BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 244 274 481 511 562 592 1705 1735
Size: 244 30 207 30 51 30 1113 30
Cuts at: 1735 4459 4489 6012 6042 6159
Size: 2724 30 1523 30 117
Fragments arranged by size:
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30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2959 4291 5440 5476 6159
Size: 2959 1332 1149 36 683
Fragments arranged by size:
2959 1332 1149 683 36
BseYI C'CCAG_C
Cuts at: 0 157 1516 1742 2195 2462 2723 3268 3517
Size: 157 1359 226 453 267 261 545 249
Cuts at: 3517 3712 3864 5312 5937 5955 5995 6016 6159
Size: 195 152 1448 625 18 40 21 143
Fragments arranged by size:
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226 195 157 152 143 40 21 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 475 723 1711 1743 2412 4073 6159
Size: 291 184 248 988 32 669 1661 2086
Fragments arranged by size:
2086 1661 988 669 291 248 184 32
BsiEI CG_ry'CG
Cuts at: 0 146 3816 6159
Size: 146 3670 2343
Fragments arranged by size:
3670 2343 146
BsiHKAI G_wGCw'C
Cuts at: 0 1456 1957 2126 2930 3545 4090 5137 5578
Size: 1456 501 169 804 615 545 1047 441
Cuts at: 5578 6094 6159
Size: 516 65
Fragments arranged by size:
1456 1047 804 615 545 516 501 441
169 65
BsiWI C'GTAC_G
Cuts at: 0 3528 4833 6159
Size: 3528 1305 1326
Fragments arranged by size:
3528 1326 1305
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1099 1236 1980 2550 3718 5109 6159
Size: 1099 137 744 570 1168 1391 1050
Fragments arranged by size:
1391 1168 1099 1050 744 570 137
Bsp1286I G_dGCh'C
Cuts at: 0 137 154 343 642 1011 1021 1097 1326
Size: 137 17 189 299 369 10 76 229
Cuts at: 1326 1340 1456 1787 1957 2066 2126 2216 2660
Size: 14 116 331 170 109 60 90 444
Cuts at: 2660 2930 2966 3227 3269 3297 3341 3545 3892
Size: 270 36 261 42 28 44 204 347
Cuts at: 3892 4016 4090 4387 4436 4957 5137 5311 5578
Size: 124 74 297 49 521 180 174 267
Cuts at: 5578 5663 5756 5880 6046 6067 6094 6159
Size: 85 93 124 166 21 27 65
Fragments arranged by size:
521 444 369 347 331 299 297 270
267 261 229 204 189 180 174 170
166 137 124 124 116 109 93 90
85 76 74 65 60 49 44 42
36 28 27 21 17 14 10
BspEI T'CCGG_A
Cuts at: 0 335 5789 6159
Size: 335 5454 370
Fragments arranged by size:
5454 370 335
BspHI T'CATG_A
Cuts at: 0 1949 6159
Size: 1949 4210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 90 2720 4505 5280 5708 6159
Size: 90 2630 1785 775 428 451
Fragments arranged by size:
2630 1785 775 451 428 90
BsrBI CCG'CTC
Cuts at: 0 2186 2445 2953 3450 6159
Size: 2186 259 508 497 2709
Fragments arranged by size:
2709 2186 508 497 259
BsrDI GCAATG_nn'
Cuts at: 0 639 1199 4686 6159
Size: 639 560 3487 1473
Fragments arranged by size:
3487 1473 639 560
BsrFI r'CCGG_y
Cuts at: 0 325 715 788 976 1448 3705 3811 4016
Size: 325 390 73 188 472 2257 106 205
Cuts at: 4016 4550 5225 5941 6159
Size: 534 675 716 218
Fragments arranged by size:
2257 716 675 534 472 390 325 218
205 188 106 73
BsrGI T'GTAC_A
Cuts at: 0 545 2870 6159
Size: 545 2325 3289
Fragments arranged by size:
3289 2325 545
BssSI C'ACGA_G
Cuts at: 0 6032 6159
Size: 6032 127
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3783 6159
Size: 3783 2376
BstBI TT'CG_AA
Cuts at: 0 1805 5733 6159
Size: 1805 3928 426
Fragments arranged by size:
3928 1805 426
BstEII G'GTnAC_C
Cuts at: 0 102 885 1314 3012 3837 6159
Size: 102 783 429 1698 825 2322
Fragments arranged by size:
2322 1698 825 783 429 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 120 684 2847 3255 4033 4542 6159
Size: 120 564 2163 408 778 509 1617
Fragments arranged by size:
2163 1617 778 564 509 408 120
BstYI r'GATC_y
Cuts at: 0 332 1427 1964 2234 2357 2681 3945 6159
Size: 332 1095 537 270 123 324 1264 2214
Fragments arranged by size:
2214 1264 1095 537 332 324 270 123
BtgI C'CryG_G
Cuts at: 0 119 146 311 534 725 1089 1310 2574
Size: 119 27 165 223 191 364 221 1264
Cuts at: 2574 2969 3131 3768 4004 4422 4957 5303 5762
Size: 395 162 637 236 418 535 346 459
Cuts at: 5762 6159
Size: 397
Fragments arranged by size:
1264 637 535 459 418 397 395 364
346 236 223 221 191 165 162 119
27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1992 2379 2499 6159
Size: 1992 387 120 3660
Fragments arranged by size:
3660 1992 387 120
BtsI GCAGTG_nn'
Cuts at: 0 1482 1731 2916 3543 3621 4039 4061 4819
Size: 1482 249 1185 627 78 418 22 758
Cuts at: 4819 6159
Size: 1340
Fragments arranged by size:
1482 1340 1185 758 627 418 249 78
22
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4086 4121 6159
Size: 4086 35 2038
Fragments arranged by size:
4086 2038 35
DraIII CAC_nnn'GTG
Cuts at: 0 726 2211 2440 5498 5776 6159
Size: 726 1485 229 3058 278 383
Fragments arranged by size:
3058 1485 726 383 278 229
DrdI GACnn_nn'nnGTC
Cuts at: 0 2542 3145 3319 6159
Size: 2542 603 174 2840
Fragments arranged by size:
2840 2542 603 174
EaeI y'GGCC_r
Cuts at: 0 143 182 422 717 786 1289 1827 2223
Size: 143 39 240 295 69 503 538 396
Cuts at: 2223 2363 2904 3317 3699 3794 3813 3989 4091
Size: 140 541 413 382 95 19 176 102
Cuts at: 4091 4802 5167 5921 6159
Size: 711 365 754 238
Fragments arranged by size:
754 711 541 538 503 413 396 382
365 295 240 238 176 143 140 102
95 69 39 19
EagI C'GGCC_G
Cuts at: 0 143 3813 6159
Size: 143 3670 2346
Fragments arranged by size:
3670 2346 143
EarI CTCTTCn'nnn_
Cuts at: 0 453 1126 2270 3234 3748 4465 4831 4837
Size: 453 673 1144 964 514 717 366 6
Cuts at: 4837 5418 6159
Size: 581 741
Fragments arranged by size:
1144 964 741 717 673 581 514 453
366 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 258 2251 6159
Size: 258 1993 3908
Fragments arranged by size:
3908 1993 258
EcoICRI GAG'CTC
Cuts at: 0 1955 2928 6159
Size: 1955 973 3231
Fragments arranged by size:
3231 1955 973
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 434 991 1224 1908 2575 2577 2698
Size: 74 360 557 233 684 667 2 121
Cuts at: 2698 3277 4287 4425 4564 4882 5479 6051 6159
Size: 579 1010 138 139 318 597 572 108
Fragments arranged by size:
1010 684 667 597 579 572 557 360
318 233 139 138 121 108 74 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2275 2299 4937 5362 6159
Size: 2275 24 2638 425 797
Fragments arranged by size:
2638 2275 797 425 24
EcoO109I rG'GnC_Cy
Cuts at: 0 1145 1323 1420 1670 2963 3223 3224 3294
Size: 1145 178 97 250 1293 260 1 70
Cuts at: 3294 3574 3861 4562 4578 4953 5140 5876 5877
Size: 280 287 701 16 375 187 736 1
Cuts at: 5877 6042 6131 6159
Size: 165 89 28
Fragments arranged by size:
1293 1145 736 701 375 287 280 260
250 187 178 165 97 89 70 28
16 1 1
EcoRI G'AATT_C
Cuts at: 0 1807 1945 6159
Size: 1807 138 4214
Fragments arranged by size:
4214 1807 138
FseI GG_CCGG'CC
Cuts at: 0 719 792 6159
Size: 719 73 5367
Fragments arranged by size:
5367 719 73
FspI TGC'GCA
Cuts at: 0 4648 6159
Size: 4648 1511
HaeII r_GCGC'y
Cuts at: 0 1301 2046 4314 6002 6159
Size: 1301 745 2268 1688 157
Fragments arranged by size:
2268 1688 1301 745 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 388 420 481 513 562 594 1892 1924
Size: 388 32 61 32 49 32 1298 32
Cuts at: 1924 5132 5164 5503 5535 6159
Size: 3208 32 339 32 624
Fragments arranged by size:
3208 1298 624 388 339 61 49 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1257 4128 5079 6159
Size: 1257 2871 951 1080
Fragments arranged by size:
2871 1257 1080 951
HindIII A'AGCT_T
Cuts at: 0 4920 6159
Size: 4920 1239
KasI G'GCGC_C
Cuts at: 0 1297 6159
Size: 1297 4862
KpnI G_GTAC'C
Cuts at: 0 180 6159
Size: 180 5979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1364 2664 5315 6159
Size: 1364 1300 2651 844
Fragments arranged by size:
2651 1364 1300 844
MscI TGG'CCA
Cuts at: 0 184 3701 4804 5923 6159
Size: 184 3517 1103 1119 236
Fragments arranged by size:
3517 1119 1103 236 184
MslI CAynn'nnrTG
Cuts at: 0 293 925 1281 2881 3010 3997 4031 4933
Size: 293 632 356 1600 129 987 34 902
Cuts at: 4933 5047 5152 6055 6154 6159
Size: 114 105 903 99 5
Fragments arranged by size:
1600 987 903 902 632 356 293 129
114 105 99 34 5
MspA1I CmG'CkG
Cuts at: 0 148 313 773 812 866 1040 1833 2462
Size: 148 165 460 39 54 174 793 629
Cuts at: 2462 2569 2597 3770 4989 5469 5884 6159
Size: 107 28 1173 1219 480 415 275
Fragments arranged by size:
1219 1173 793 629 480 460 415 275
174 165 148 107 54 39 28
NaeI GCC'GGC
Cuts at: 0 327 717 790 3707 3813 5943 6159
Size: 327 390 73 2917 106 2130 216
Fragments arranged by size:
2917 2130 390 327 216 106 73
NarI GG'CG_CC
Cuts at: 0 1298 6159
Size: 1298 4861
NcoI C'CATG_G
Cuts at: 0 2969 4004 6159
Size: 2969 1035 2155
Fragments arranged by size:
2969 2155 1035
NgoMIV G'CCGG_C
Cuts at: 0 325 715 788 3705 3811 5941 6159
Size: 325 390 73 2917 106 2130 218
Fragments arranged by size:
2917 2130 390 325 218 106 73
NheI G'CTAG_C
Cuts at: 0 3610 6159
Size: 3610 2549
NsiI A_TGCA'T
Cuts at: 0 2008 6159
Size: 2008 4151
NspI r_CATG'y
Cuts at: 0 1872 2006 2176 2886 4486 5157 5452 6159
Size: 1872 134 170 710 1600 671 295 707
Fragments arranged by size:
1872 1600 710 707 671 295 170 134
PasI CC'CwG_GG
Cuts at: 0 4950 4979 5872 6159
Size: 4950 29 893 287
Fragments arranged by size:
4950 893 287 29
PciI A'CATG_T
Cuts at: 0 5153 6159
Size: 5153 1006
PflMI CCAn_nnn'nTGG
Cuts at: 0 726 773 1040 1610 3501 6159
Size: 726 47 267 570 1891 2658
Fragments arranged by size:
2658 1891 726 570 267 47
PfoI T'CCnGG_A
Cuts at: 0 26 683 2684 5188 6159
Size: 26 657 2001 2504 971
Fragments arranged by size:
2504 2001 971 657 26
PmlI CAC'GTG
Cuts at: 0 4085 4346 4634 6159
Size: 4085 261 288 1525
Fragments arranged by size:
4085 1525 288 261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5215 5247 6159
Size: 5215 32 912
Fragments arranged by size:
5215 912 32
PpuMI rG'GwC_Cy
Cuts at: 0 1670 3574 4562 5140 6159
Size: 1670 1904 988 578 1019
Fragments arranged by size:
1904 1670 1019 988 578
PspOMI G'GGCC_C
Cuts at: 0 1322 2962 3223 3293 4953 5876 6042 6159
Size: 1322 1640 261 70 1660 923 166 117
Fragments arranged by size:
1660 1640 1322 923 261 166 117 70
PstI C_TGCA'G
Cuts at: 0 248 1579 1964 3044 4502 5474 6159
Size: 248 1331 385 1080 1458 972 685
Fragments arranged by size:
1458 1331 1080 972 685 385 248
PvuII CAG'CTG
Cuts at: 0 1833 2597 4989 5469 6159
Size: 1833 764 2392 480 690
Fragments arranged by size:
2392 1833 764 690 480
RsrII CG'GwC_CG
Cuts at: 0 854 5637 6159
Size: 854 4783 522
Fragments arranged by size:
4783 854 522
SacI G_AGCT'C
Cuts at: 0 1957 2930 6159
Size: 1957 973 3229
Fragments arranged by size:
3229 1957 973
SacII CC_GC'GG
Cuts at: 0 149 314 3771 6159
Size: 149 165 3457 2388
Fragments arranged by size:
3457 2388 165 149
SanDI GG'GwC_CC
Cuts at: 0 1670 6159
Size: 1670 4489
SapI GCTCTTCn'nnn_
Cuts at: 0 1126 3748 4831 6159
Size: 1126 2622 1083 1328
Fragments arranged by size:
2622 1328 1126 1083
SbfI CC_TGCA'GG
Cuts at: 0 4502 6159
Size: 4502 1657
ScaI AGT'ACT
Cuts at: 0 4258 6159
Size: 4258 1901
SfcI C'TryA_G
Cuts at: 0 244 1575 1960 3040 3075 3486 4252 4498
Size: 244 1331 385 1080 35 411 766 246
Cuts at: 4498 5346 5470 6159
Size: 848 124 689
Fragments arranged by size:
1331 1080 848 766 689 411 385 246
244 124 35
SfoI GGC'GCC
Cuts at: 0 1299 6159
Size: 1299 4860
SmaI CCC'GGG
Cuts at: 0 1263 3693 5437 6159
Size: 1263 2430 1744 722
Fragments arranged by size:
2430 1744 1263 722
SmlI C'TyrA_G
Cuts at: 0 778 1207 1675 1981 2560 3166 4333 4745
Size: 778 429 468 306 579 606 1167 412
Cuts at: 4745 5232 6082 6159
Size: 487 850 77
Fragments arranged by size:
1167 850 778 606 579 487 468 429
412 306 77
SphI G_CATG'C
Cuts at: 0 2176 4486 5452 6159
Size: 2176 2310 966 707
Fragments arranged by size:
2310 2176 966 707
SrfI GCCC'GGGC
Cuts at: 0 5437 6159
Size: 5437 722
StuI AGG'CCT
Cuts at: 0 5263 5725 5782 6159
Size: 5263 462 57 377
Fragments arranged by size:
5263 462 377 57
StyI C'CwwG_G
Cuts at: 0 1292 2156 2177 2522 2969 3557 3904 3971
Size: 1292 864 21 345 447 588 347 67
Cuts at: 3971 4004 4556 5855 6159
Size: 33 552 1299 304
Fragments arranged by size:
1299 1292 864 588 552 447 347 345
304 67 33 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 871 1530 2169 3042 3526 3780 4480 4746
Size: 871 659 639 873 484 254 700 266
Cuts at: 4746 5132 5634 5830 5885 6000 6159
Size: 386 502 196 55 115 159
Fragments arranged by size:
873 871 700 659 639 502 484 386
266 254 196 159 115 55
TatI w'GTAC_w
Cuts at: 0 545 1373 2870 2885 3107 3668 4256 5095
Size: 545 828 1497 15 222 561 588 839
Cuts at: 5095 6159
Size: 1064
Fragments arranged by size:
1497 1064 839 828 588 561 545 222
15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1505 1537 1705 1737 2990 3022 4200 4232
Size: 1505 32 168 32 1253 32 1178 32
Cuts at: 4232 4459 4491 6012 6044 6159
Size: 227 32 1521 32 115
Fragments arranged by size:
1521 1505 1253 1178 227 168 115 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 493 2479 2497 2539 6159
Size: 493 1986 18 42 3620
Fragments arranged by size:
3620 1986 493 42 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4377 4812 5793 5853 6159
Size: 4377 435 981 60 306
Fragments arranged by size:
4377 981 435 306 60
XhoI C'TCGA_G
Cuts at: 0 6082 6159
Size: 6082 77
XmaI C'CCGG_G
Cuts at: 0 1261 3691 5435 6159
Size: 1261 2430 1744 724
Fragments arranged by size:
2430 1744 1261 724
XmnI GAAnn'nnTTC
Cuts at: 0 4774 6159
Size: 4774 1385
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
RsrII SacI SacII SanDI SapI SbfI ScaI SfcI
SfoI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AhdI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BsaBI BsmI BssHII BstZ17I Bsu36I
ClaI DraI EcoRV FalI FspAI HpaI MfeI MluI
NdeI NotI NruI PacI PmeI PshAI PsiI PspXI
PsrI PvuI SalI SexAI SfiI SgfI SgrAI SnaBI
SpeI SspI SwaI XbaI ZraI