(Circular) (Six-base) MAPSORT of: pFN21AA1128.seq Check: 5228 from: 1 to: 7846 pFN21AA1128 7846 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3040 3040 Size: 7846 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5656 278 Size: 53 83 186 936 4120 2468 Fragments arranged by size: 4120 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4695 5189 1937 Size: 2758 494 4594 Fragments arranged by size: 4594 2758 494 Acc65I G'GTAC_C Cuts at: 4798 5167 4798 Size: 369 7477 AclI AA'CG_TT Cuts at: 1563 5973 6346 1563 Size: 4410 373 3063 Fragments arranged by size: 4410 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3367 4217 4596 4652 4871 5909 7293 7561 Size: 1735 850 379 56 219 1038 1384 268 Cuts at: 7561 1632 Size: 1917 Fragments arranged by size: 1917 1735 1384 1038 850 379 268 219 56 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7649 AflIII A'CryG_T Cuts at: 1203 2296 3041 3537 4480 4868 5143 6745 7722 Size: 1093 745 496 943 388 275 1602 977 Cuts at: 7722 1203 Size: 1327 Fragments arranged by size: 1602 1327 1093 977 943 745 496 388 275 AgeI A'CCGG_T Cuts at: 6677 6677 Size: 7846 AhdI GACnn_n'nnGTC Cuts at: 6575 6575 Size: 7846 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7846 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7846 AlwNI CAG_nnn'CTG Cuts at: 4814 4930 5589 7161 4814 Size: 116 659 1572 5499 Fragments arranged by size: 5499 1572 659 116 ApaLI G'TGCA_C Cuts at: 2229 4455 5903 7059 2229 Size: 2226 1448 1156 3016 Fragments arranged by size: 3016 2226 1448 1156 ApoI r'AATT_y Cuts at: 1493 2263 2456 2692 2821 3025 4323 5155 5381 Size: 770 193 236 129 204 1298 832 226 Cuts at: 5381 5533 7642 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1298 832 770 236 226 204 193 152 129 AseI AT'TA_AT Cuts at: 160 1032 2643 6400 160 Size: 872 1611 3757 1606 Fragments arranged by size: 3757 1611 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7846 AvaI C'yCGr_G Cuts at: 1870 1943 4679 5171 1870 Size: 73 2736 492 4545 Fragments arranged by size: 4545 2736 492 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3160 3193 3160 Size: 33 7813 BamHI G'GATC_C Cuts at: 5176 5543 5176 Size: 367 7479 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4798 5167 6622 618 Size: 325 217 627 3011 369 1455 1842 Fragments arranged by size: 3011 1842 1455 627 369 325 217 BanII G_rGCy'C Cuts at: 1391 5165 1391 Size: 3774 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3246 3276 5117 928 Size: 971 1347 30 1841 3657 Fragments arranged by size: 3657 1841 1347 971 30 BbvCI CC'TCA_GC Cuts at: 3824 3824 Size: 7846 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6037 6071 1903 Size: 34 4100 34 3678 Fragments arranged by size: 4100 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5738 6948 5738 Size: 1210 6636 BclI T'GATC_A Cuts at: 1548 1548 Size: 7846 BfrBI ATG'CAT Cuts at: 4444 4444 Size: 7846 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6457 136 Size: 107 122 71 631 605 4785 1525 Fragments arranged by size: 4785 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7841 7841 Size: 7846 BlpI GC'TnA_GC Cuts at: 2611 5263 2611 Size: 2652 5194 Bme1580I G_kGCm'C Cuts at: 1790 2233 4459 5907 7063 1790 Size: 443 2226 1448 1156 2573 Fragments arranged by size: 2573 2226 1448 1156 443 BmgBI CAC'GTC Cuts at: 1318 4483 1318 Size: 3165 4681 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5577 6535 442 Size: 416 53 83 399 24 4160 958 1753 Fragments arranged by size: 4160 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7846 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2622 3279 4336 4791 6506 1239 Size: 1383 657 1057 455 1715 2579 Fragments arranged by size: 2579 1715 1383 1057 657 455 Bpu10I CC'TnA_GC Cuts at: 3072 3824 3072 Size: 752 7094 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2292 3671 3886 4317 4590 5971 6836 7134 2292 Size: 1379 215 431 273 1381 865 298 3004 Fragments arranged by size: 3004 1381 1379 865 431 298 273 215 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6509 882 Size: 617 5010 2219 Fragments arranged by size: 5010 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5637 493 Size: 711 4433 2702 Fragments arranged by size: 4433 2702 711 BsaBI GATnn'nnATC Cuts at: 3660 3678 7840 3660 Size: 18 4162 3666 Fragments arranged by size: 4162 3666 18 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5653 6035 6670 275 Size: 53 83 186 936 4120 382 635 1451 Fragments arranged by size: 4120 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4462 6279 6677 6951 7098 7509 4462 Size: 1817 398 274 147 411 4799 Fragments arranged by size: 4799 1817 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3418 3448 1178 Size: 30 165 30 2015 30 5576 Fragments arranged by size: 5576 2015 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2426 4320 1186 Size: 1240 1894 4712 Fragments arranged by size: 4712 1894 1240 BseYI C'CCAG_C Cuts at: 7049 7545 7049 Size: 496 7350 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7273 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5296 6057 7085 1540 Size: 275 186 3295 761 1028 2301 Fragments arranged by size: 3295 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2233 4459 5907 5992 7063 2233 Size: 2226 1448 85 1071 3016 Fragments arranged by size: 3016 2226 1448 1071 85 BsmI GAATG_Cn' Cuts at: 4430 5086 5357 5450 4430 Size: 656 271 93 6826 Fragments arranged by size: 6826 656 271 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4061 4312 4061 Size: 251 7595 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 4459 5165 5907 5992 7063 1391 Size: 399 443 2226 706 742 85 1071 2174 Fragments arranged by size: 2226 2174 1071 742 706 443 399 85 BspEI T'CCGG_A Cuts at: 4462 7509 4462 Size: 3047 4799 BspHI T'CATG_A Cuts at: 5735 7465 5735 Size: 1730 6116 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3040 5201 844 Size: 2196 2161 3489 Fragments arranged by size: 3489 2196 2161 BsrBI CCG'CTC Cuts at: 1394 1823 5733 1394 Size: 429 3910 3507 Fragments arranged by size: 3910 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2081 4085 4106 6341 6515 65 Size: 1183 833 2004 21 2235 174 1396 Fragments arranged by size: 2235 2004 1396 1183 833 174 21 BsrFI r'CCGG_y Cuts at: 1700 1810 6490 6677 1700 Size: 110 4680 187 2869 Fragments arranged by size: 4680 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3434 96 Size: 3338 4508 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7846 BssSI C'ACGA_G Cuts at: 5906 6918 5906 Size: 1012 6834 BstAPI GCAn_nnn'nTGC Cuts at: 794 2092 4425 794 Size: 1298 2333 4215 Fragments arranged by size: 4215 2333 1298 BstBI TT'CG_AA Cuts at: 6650 6650 Size: 7846 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7846 BstXI CCAn_nnnn'nTGG Cuts at: 3584 3584 Size: 7846 BstYI r'GATC_y Cuts at: 1972 2749 3679 4215 5176 5543 5928 5945 7397 Size: 777 930 536 961 367 385 17 1452 Cuts at: 7397 7483 7495 7841 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 961 930 777 536 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3687 3765 4957 3687 Size: 78 1192 6576 Fragments arranged by size: 6576 1192 78 BtgI C'CryG_G Cuts at: 513 1065 2004 3850 513 Size: 552 939 1846 4509 Fragments arranged by size: 4509 1846 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7107 Fragments arranged by size: 7107 683 56 BtsI GCAGTG_nn' Cuts at: 4020 4426 5362 6156 6176 4020 Size: 406 936 794 20 5690 Fragments arranged by size: 5690 936 794 406 20 ClaI AT'CG_AT Cuts at: 1554 5113 1554 Size: 3559 4287 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2377 2412 4536 4571 529 Size: 35 1813 35 2124 35 3804 Fragments arranged by size: 3804 2124 1813 35 35 35 DraI TTT'AAA Cuts at: 4773 5150 4773 Size: 377 7469 DraIII CAC_nnn'GTG Cuts at: 2237 2237 Size: 7846 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6853 809 Size: 507 5537 1802 Fragments arranged by size: 5537 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5293 6182 7811 Size: 54 1425 50 275 18 3463 889 1629 Cuts at: 7811 8 Size: 43 Fragments arranged by size: 3463 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5293 1537 Size: 275 3481 4090 Fragments arranged by size: 4090 3481 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2238 3312 4509 4671 5776 1353 Size: 885 1074 1197 162 1105 3423 Fragments arranged by size: 3423 1197 1105 1074 885 162 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4719 4937 6433 6807 6953 387 Size: 1064 138 3130 218 1496 374 146 1280 Fragments arranged by size: 3130 1496 1280 1064 374 218 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2622 3279 3367 4217 4336 4596 4652 Size: 393 990 657 88 850 119 260 56 Cuts at: 4652 4791 4871 5909 6506 7293 7561 1239 Size: 139 80 1038 597 787 268 1524 Fragments arranged by size: 1524 1038 990 850 787 657 597 393 268 260 139 119 88 80 56 EcoNI CCTnn'n_nnAGG Cuts at: 2307 3768 2307 Size: 1461 6385 EcoRI G'AATT_C Cuts at: 2692 4323 5155 5533 2692 Size: 1631 832 378 5005 Fragments arranged by size: 5005 1631 832 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2373 2373 Size: 7846 FspI TGC'GCA Cuts at: 6352 6352 Size: 7846 HaeII r_GCGC'y Cuts at: 1678 6993 1678 Size: 5315 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2491 2523 3290 Size: 32 135 32 593 32 294 32 767 Cuts at: 3290 3322 6487 6519 6561 6593 1373 Size: 32 3165 32 42 32 2626 Fragments arranged by size: 3165 2626 767 593 294 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5190 5436 669 Size: 988 281 3252 246 3079 Fragments arranged by size: 3252 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2608 4471 5008 748 Size: 1860 1863 537 3586 Fragments arranged by size: 3586 1863 1860 537 HpaI GTT'AAC Cuts at: 5436 5436 Size: 7846 KpnI G_GTAC'C Cuts at: 4802 5171 4802 Size: 369 7477 MfeI C'AATT_G Cuts at: 5445 5445 Size: 7846 MluI A'CGCG_T Cuts at: 7722 7722 Size: 7846 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3300 4243 4693 6960 7144 1305 Size: 1995 943 450 2267 184 2007 Fragments arranged by size: 2267 2007 1995 943 450 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6024 Fragments arranged by size: 6024 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2789 3330 3538 3849 4068 5804 6163 Size: 648 1623 541 208 311 219 1736 359 Cuts at: 6163 6322 518 Size: 159 2042 Fragments arranged by size: 2042 1736 1623 648 541 359 311 219 208 159 MspA1I CmG'CkG Cuts at: 1606 2549 5262 5939 7087 7332 1606 Size: 943 2713 677 1148 245 2120 Fragments arranged by size: 2713 2120 1148 943 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3850 513 Size: 552 939 1846 4509 Fragments arranged by size: 4509 1846 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7846 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7846 NotI GC'GGCC_GC Cuts at: 5293 5293 Size: 7846 NsiI A_TGCA'T Cuts at: 4446 4446 Size: 7846 NspI r_CATG'y Cuts at: 1132 1229 2560 3045 3541 4444 4648 4872 5147 Size: 97 1331 485 496 903 204 224 275 Cuts at: 5147 5204 6749 1132 Size: 57 1545 2229 Fragments arranged by size: 2229 1545 1331 903 496 485 275 224 204 97 57 PasI CC'CwG_GG Cuts at: 1348 4381 1348 Size: 3033 4813 PciI A'CATG_T Cuts at: 3041 3537 4868 5143 6745 3041 Size: 496 1331 275 1602 4142 Fragments arranged by size: 4142 1602 1331 496 275 PflMI CCAn_nnn'nTGG Cuts at: 1108 2057 2996 4133 1108 Size: 949 939 1137 4821 Fragments arranged by size: 4821 1137 949 939 PmeI GTTT'AAAC Cuts at: 5150 5150 Size: 7846 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5915 5947 7428 7460 5915 Size: 32 1481 32 6301 Fragments arranged by size: 6301 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7846 PsiI TTA'TAA Cuts at: 5416 5416 Size: 7846 PstI C_TGCA'G Cuts at: 830 5198 830 Size: 4368 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7846 PvuII CAG'CTG Cuts at: 2549 2549 Size: 7846 SalI G'TCGA_C Cuts at: 1936 5188 1936 Size: 3252 4594 SapI GCTCTTCn'nnn_ Cuts at: 2238 4509 2238 Size: 2271 5575 SbfI CC_TGCA'GG Cuts at: 5198 5198 Size: 7846 ScaI AGT'ACT Cuts at: 2841 4338 6094 7806 2841 Size: 1497 1756 1712 2881 Fragments arranged by size: 2881 1756 1712 1497 SfcI C'TryA_G Cuts at: 826 1045 2406 3060 3481 3883 4340 5194 6329 Size: 219 1361 654 421 402 457 854 1135 Cuts at: 6329 7010 7201 826 Size: 681 191 1471 Fragments arranged by size: 1471 1361 1135 854 681 654 457 421 402 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7846 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7846 SmaI CCC'GGG Cuts at: 1872 5173 1872 Size: 3301 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2307 3650 3865 4332 4605 5950 Size: 197 926 364 1343 215 467 273 1345 Cuts at: 5950 6851 7113 820 Size: 901 262 1553 Fragments arranged by size: 1553 1345 1343 926 901 467 364 273 262 215 197 SnaBI TAC'GTA Cuts at: 493 5637 493 Size: 5144 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7846 SphI G_CATG'C Cuts at: 1132 2560 5204 1132 Size: 1428 2644 3774 Fragments arranged by size: 3774 2644 1428 SspI AAT'ATT Cuts at: 5 52 5770 5 Size: 47 5718 2081 Fragments arranged by size: 5718 2081 47 StuI AGG'CCT Cuts at: 1517 2566 2881 1517 Size: 1049 315 6482 Fragments arranged by size: 6482 1049 315 StyI C'CwwG_G Cuts at: 513 1065 2004 3363 3850 5033 5285 513 Size: 552 939 1359 487 1183 252 3074 Fragments arranged by size: 3074 1359 1183 939 552 487 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3753 4394 5873 5890 6043 6228 6673 Size: 1255 2173 641 1479 17 153 185 445 Cuts at: 6673 325 Size: 1498 Fragments arranged by size: 2173 1498 1479 1255 641 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2078 2839 3434 Size: 274 80 33 51 1443 101 761 595 Cuts at: 3434 4336 4565 4688 6092 7804 96 Size: 902 229 123 1404 1712 138 Fragments arranged by size: 1712 1443 1404 902 761 595 274 229 138 123 101 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7806 7838 7806 Size: 32 7814 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7846 XbaI T'CTAG_A Cuts at: 5182 5182 Size: 7846 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3795 3800 3795 Size: 5 7841 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7846 XmaI C'CCGG_G Cuts at: 1870 5171 1870 Size: 3301 4545 XmnI GAAnn'nnTTC Cuts at: 2485 2596 2696 4081 5975 2485 Size: 111 100 1385 1894 4356 Fragments arranged by size: 4356 1894 1385 111 100 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5654 276 Size: 53 83 186 936 4120 2468 Fragments arranged by size: 4120 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BstZ17I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI