(Circular) (Six-base) MAPSORT of: pFN21AA1116.seq Check: 4052 from: 1 to: 8518 pFN21AA1116 8518 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6328 278 Size: 53 83 186 936 4792 2468 Fragments arranged by size: 4792 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4977 5089 5861 1937 Size: 3040 112 772 4594 Fragments arranged by size: 4594 3040 772 112 Acc65I G'GTAC_C Cuts at: 5839 5839 Size: 8518 AclI AA'CG_TT Cuts at: 1563 6645 7018 1563 Size: 5082 373 3063 Fragments arranged by size: 5082 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3099 3575 4323 6581 7965 8233 1632 Size: 1467 476 748 2258 1384 268 1917 Fragments arranged by size: 2258 1917 1467 1384 748 476 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8321 AflIII A'CryG_T Cuts at: 1203 2128 7417 8394 1203 Size: 925 5289 977 1327 Fragments arranged by size: 5289 1327 977 925 AgeI A'CCGG_T Cuts at: 5052 5326 7349 5052 Size: 274 2023 6221 Fragments arranged by size: 6221 2023 274 AhdI GACnn_n'nnGTC Cuts at: 7247 7247 Size: 8518 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8518 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5421 5453 5421 Size: 32 8486 AlwNI CAG_nnn'CTG Cuts at: 2885 3824 4301 5768 5807 6261 7833 2885 Size: 939 477 1467 39 454 1572 3570 Fragments arranged by size: 3570 1572 1467 939 477 454 39 ApaLI G'TGCA_C Cuts at: 6575 7731 6575 Size: 1156 7362 ApoI r'AATT_y Cuts at: 1493 2296 2850 4437 4479 4839 5550 5827 6053 Size: 803 554 1587 42 360 711 277 226 Cuts at: 6053 6205 8314 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1587 803 711 554 360 277 226 152 42 AseI AT'TA_AT Cuts at: 160 1032 2621 3522 7072 160 Size: 872 1589 901 3550 1606 Fragments arranged by size: 3550 1606 1589 901 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8518 AvaI C'yCGr_G Cuts at: 1870 1943 3537 5583 5843 1870 Size: 73 1594 2046 260 4545 Fragments arranged by size: 4545 2046 1594 260 73 BamHI G'GATC_C Cuts at: 5848 6215 5848 Size: 367 8151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3940 4804 5839 7294 618 Size: 325 217 627 2153 864 1035 1455 1842 Fragments arranged by size: 2153 1842 1455 1035 864 627 325 217 BanII G_rGCy'C Cuts at: 1391 3589 3857 5837 1391 Size: 2198 268 1980 4072 Fragments arranged by size: 4072 2198 1980 268 BbeI G_GCGC'C Cuts at: 3944 3944 Size: 8518 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2024 2823 4972 5194 5470 928 Size: 971 125 799 2149 222 276 3976 Fragments arranged by size: 3976 2149 971 799 276 222 125 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6709 6743 1903 Size: 34 4772 34 3678 Fragments arranged by size: 4772 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4751 4998 6410 7620 4751 Size: 247 1412 1210 5649 Fragments arranged by size: 5649 1412 1210 247 BclI T'GATC_A Cuts at: 1548 3775 1548 Size: 2227 6291 BfrBI ATG'CAT Cuts at: 3578 3578 Size: 8518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5284 7129 Size: 107 122 71 631 605 337 3275 1845 Cuts at: 7129 136 Size: 1525 Fragments arranged by size: 3275 1845 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2583 3407 8513 2583 Size: 824 5106 2588 Fragments arranged by size: 5106 2588 824 BlpI GC'TnA_GC Cuts at: 5673 5935 5673 Size: 262 8256 Bme1580I G_kGCm'C Cuts at: 1790 6579 7735 1790 Size: 4789 1156 2573 Fragments arranged by size: 4789 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8518 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4578 6249 7207 Size: 416 53 83 399 24 3161 1671 958 Cuts at: 7207 442 Size: 1753 Fragments arranged by size: 3161 1753 1671 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8518 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2490 3876 5688 2490 Size: 1386 1812 5320 Fragments arranged by size: 5320 1812 1386 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2409 2460 3846 4074 4902 7178 1239 Size: 1170 51 1386 228 828 2276 2579 Fragments arranged by size: 2579 2276 1386 1170 828 228 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2583 3181 3292 6643 7508 7806 2583 Size: 598 111 3351 865 298 3295 Fragments arranged by size: 3351 3295 865 598 298 111 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5244 7181 882 Size: 617 3745 1937 2219 Fragments arranged by size: 3745 2219 1937 617 BsaAI yAC'GTr Cuts at: 493 1204 6309 493 Size: 711 5105 2702 Fragments arranged by size: 5105 2702 711 BsaBI GATnn'nnATC Cuts at: 3696 8512 3696 Size: 4816 3702 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3941 6325 6707 7342 Size: 53 83 186 936 2408 2384 382 635 Cuts at: 7342 275 Size: 1451 Fragments arranged by size: 2408 2384 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4174 5052 5326 6951 7349 7623 7770 8181 4174 Size: 878 274 1625 398 274 147 411 4511 Fragments arranged by size: 4511 1625 878 411 398 274 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2740 2770 1178 Size: 30 165 30 1337 30 6926 Fragments arranged by size: 6926 1337 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2982 3170 3900 3993 5307 5565 1186 Size: 1796 188 730 93 1314 258 4139 Fragments arranged by size: 4139 1796 1314 730 258 188 93 BseYI C'CCAG_C Cuts at: 2655 7721 8217 2655 Size: 5066 496 2956 Fragments arranged by size: 5066 2956 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4172 1163 Size: 573 2436 5509 Fragments arranged by size: 5509 2436 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3338 5968 6729 7757 1540 Size: 275 186 1337 2630 761 1028 2301 Fragments arranged by size: 2630 2301 1337 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3589 6579 6664 7735 3589 Size: 2990 85 1071 4372 Fragments arranged by size: 4372 2990 1071 85 BsmI GAATG_Cn' Cuts at: 3028 6029 6122 3028 Size: 3001 93 5424 Fragments arranged by size: 5424 3001 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3827 3827 Size: 8518 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3589 3857 5837 6579 6664 7735 1391 Size: 399 1799 268 1980 742 85 1071 2174 Fragments arranged by size: 2174 1980 1799 1071 742 399 268 85 BspEI T'CCGG_A Cuts at: 8181 8181 Size: 8518 BspHI T'CATG_A Cuts at: 6407 8137 6407 Size: 1730 6788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5873 844 Size: 5029 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3306 4253 6405 1394 Size: 429 1483 947 2152 3507 Fragments arranged by size: 3507 2152 1483 947 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7013 7187 65 Size: 1183 5765 174 1396 Fragments arranged by size: 5765 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3994 5052 5326 7162 7349 1700 Size: 110 2184 1058 274 1836 187 2869 Fragments arranged by size: 2869 2184 1836 1058 274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8518 BssSI C'ACGA_G Cuts at: 6578 7590 6578 Size: 1012 7506 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8518 BstBI TT'CG_AA Cuts at: 2069 7322 2069 Size: 5253 3265 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8518 BstYI r'GATC_y Cuts at: 1972 2583 3407 3697 5134 5848 6215 6600 6617 Size: 611 824 290 1437 714 367 385 17 Cuts at: 6617 8069 8155 8167 8513 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1437 824 714 611 385 367 346 290 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2994 5640 2994 Size: 2646 5872 BtgI C'CryG_G Cuts at: 513 1065 2004 2534 3719 4903 513 Size: 552 939 530 1185 1184 4128 Fragments arranged by size: 4128 1185 1184 939 552 530 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7779 Fragments arranged by size: 7779 683 56 BtsI GCAGTG_nn' Cuts at: 2457 6034 6828 6848 2457 Size: 3577 794 20 4127 Fragments arranged by size: 4127 3577 794 20 ClaI AT'CG_AT Cuts at: 1554 5389 1554 Size: 3835 4683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8483 DraI TTT'AAA Cuts at: 3654 5822 3654 Size: 2168 6350 DraIII CAC_nnn'GTG Cuts at: 3422 4586 4904 3422 Size: 1164 318 7036 Fragments arranged by size: 7036 1164 318 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7525 809 Size: 507 6209 1802 Fragments arranged by size: 6209 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2445 2505 4493 Size: 54 1425 50 275 18 615 60 1988 Cuts at: 4493 5965 6854 8483 8 Size: 1472 889 1629 43 Fragments arranged by size: 1988 1629 1472 1425 889 615 275 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5965 1537 Size: 275 4153 4090 Fragments arranged by size: 4153 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3147 3492 3594 4893 5242 5747 6448 1353 Size: 1794 345 102 1299 349 505 701 3423 Fragments arranged by size: 3423 1794 1299 701 505 349 345 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2409 7105 7479 7625 387 Size: 1064 138 820 4696 374 146 1280 Fragments arranged by size: 4696 1280 1064 820 374 146 138 EcoICRI GAG'CTC Cuts at: 3587 3587 Size: 8518 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2409 2460 3099 3575 3846 4074 4323 Size: 393 777 51 639 476 271 228 249 Cuts at: 4323 4902 6581 7178 7965 8233 1239 Size: 579 1679 597 787 268 1524 Fragments arranged by size: 1679 1524 787 777 639 597 579 476 393 271 268 249 228 51 EcoNI CCTnn'n_nnAGG Cuts at: 5473 5473 Size: 8518 EcoO109I rG'GnC_Cy Cuts at: 2674 5147 2674 Size: 2473 6045 EcoRI G'AATT_C Cuts at: 4437 4839 5550 5827 6205 4437 Size: 402 711 277 378 6750 Fragments arranged by size: 6750 711 402 378 277 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4839 5427 4839 Size: 588 7930 FspI TGC'GCA Cuts at: 7024 7024 Size: 8518 HaeII r_GCGC'y Cuts at: 1678 3944 7665 1678 Size: 2266 3721 2531 Fragments arranged by size: 3721 2531 2266 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2407 2439 2943 2975 5299 Size: 32 135 32 835 32 504 32 2324 Cuts at: 5299 5331 7159 7191 7233 7265 1373 Size: 32 1828 32 42 32 2626 Fragments arranged by size: 2626 2324 1828 835 504 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4937 5862 6108 669 Size: 988 281 2999 925 246 3079 Fragments arranged by size: 3079 2999 988 925 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8518 HpaI GTT'AAC Cuts at: 6108 6108 Size: 8518 KasI G'GCGC_C Cuts at: 3940 3940 Size: 8518 KpnI G_GTAC'C Cuts at: 5843 5843 Size: 8518 MfeI C'AATT_G Cuts at: 6117 6117 Size: 8518 MluI A'CGCG_T Cuts at: 8394 8394 Size: 8518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4174 4307 7632 7816 1305 Size: 2869 133 3325 184 2007 Fragments arranged by size: 3325 2869 2007 184 133 MscI TGG'CCA Cuts at: 10 64 1489 1832 2447 10 Size: 54 1425 343 615 6081 Fragments arranged by size: 6081 1425 615 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2462 3524 6476 6835 6994 518 Size: 648 1296 1062 2952 359 159 2042 Fragments arranged by size: 2952 2042 1296 1062 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2729 2735 2741 4262 4712 5934 6611 7759 Size: 1123 6 6 1521 450 1222 677 1148 Cuts at: 7759 8004 1606 Size: 245 2120 Fragments arranged by size: 2120 1521 1222 1148 1123 677 450 245 6 6 NarI GG'CG_CC Cuts at: 3941 3941 Size: 8518 NcoI C'CATG_G Cuts at: 513 1065 2004 3719 513 Size: 552 939 1715 5312 Fragments arranged by size: 5312 1715 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8518 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8518 NotI GC'GGCC_GC Cuts at: 5965 5965 Size: 8518 NsiI A_TGCA'T Cuts at: 3580 3580 Size: 8518 NspI r_CATG'y Cuts at: 1132 1229 2132 5672 5876 7421 1132 Size: 97 903 3540 204 1545 2229 Fragments arranged by size: 3540 2229 1545 903 204 97 PasI CC'CwG_GG Cuts at: 1348 2312 5280 5469 1348 Size: 964 2968 189 4397 Fragments arranged by size: 4397 2968 964 189 PciI A'CATG_T Cuts at: 2128 7417 2128 Size: 5289 3229 PflMI CCAn_nnn'nTGG Cuts at: 1108 4073 4712 4904 5168 1108 Size: 2965 639 192 264 4458 Fragments arranged by size: 4458 2965 639 264 192 PfoI T'CCnGG_A Cuts at: 2476 4006 4684 4786 4918 4960 5149 2476 Size: 1530 678 102 132 42 189 5845 Fragments arranged by size: 5845 1530 678 189 132 102 42 PmeI GTTT'AAAC Cuts at: 5822 5822 Size: 8518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6587 6619 8100 8132 6587 Size: 32 1481 32 6973 Fragments arranged by size: 6973 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5147 5147 Size: 8518 PshAI GACnn'nnGTC Cuts at: 1613 3881 4782 1613 Size: 2268 901 5349 Fragments arranged by size: 5349 2268 901 PsiI TTA'TAA Cuts at: 6088 6088 Size: 8518 PstI C_TGCA'G Cuts at: 830 2673 2734 2740 5516 5870 830 Size: 1843 61 6 2776 354 3478 Fragments arranged by size: 3478 2776 1843 354 61 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8518 PvuII CAG'CTG Cuts at: 2729 2735 2741 4262 4712 2729 Size: 6 6 1521 450 6535 Fragments arranged by size: 6535 1521 450 6 6 SacI G_AGCT'C Cuts at: 3589 3589 Size: 8518 SalI G'TCGA_C Cuts at: 1936 5860 1936 Size: 3924 4594 SapI GCTCTTCn'nnn_ Cuts at: 3594 4893 3594 Size: 1299 7219 SbfI CC_TGCA'GG Cuts at: 5870 5870 Size: 8518 ScaI AGT'ACT Cuts at: 2343 3433 6766 8478 2343 Size: 1090 3333 1712 2383 Fragments arranged by size: 3333 2383 1712 1090 SexAI A'CCwGG_T Cuts at: 4072 4072 Size: 8518 SfcI C'TryA_G Cuts at: 826 1045 2574 2669 2730 2736 3954 5064 5130 Size: 219 1529 95 61 6 1218 1110 66 Cuts at: 5130 5196 5512 5866 7001 7682 7873 826 Size: 66 316 354 1135 681 191 1471 Fragments arranged by size: 1529 1471 1218 1135 1110 681 354 316 219 191 95 66 66 61 6 SfoI GGC'GCC Cuts at: 3942 3942 Size: 8518 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8518 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8518 SmaI CCC'GGG Cuts at: 1872 3539 5845 1872 Size: 1667 2306 4545 Fragments arranged by size: 4545 2306 1667 SmlI C'TyrA_G Cuts at: 820 1017 1943 2598 3196 3307 6622 7523 7785 Size: 197 926 655 598 111 3315 901 262 Cuts at: 7785 820 Size: 1553 Fragments arranged by size: 3315 1553 926 901 655 598 262 197 111 SnaBI TAC'GTA Cuts at: 493 6309 493 Size: 5816 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8518 SphI G_CATG'C Cuts at: 1132 5876 1132 Size: 4744 3774 SspI AAT'ATT Cuts at: 5 52 4546 4566 6442 5 Size: 47 4494 20 1876 2081 Fragments arranged by size: 4494 2081 1876 47 20 StuI AGG'CCT Cuts at: 1517 1517 Size: 8518 StyI C'CwwG_G Cuts at: 513 1065 2004 2518 3627 3719 5411 5957 513 Size: 552 939 514 1109 92 1692 546 3074 Fragments arranged by size: 3074 1692 1109 939 552 546 514 92 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2077 2275 4597 5364 6545 6562 6715 Size: 1255 497 198 2322 767 1181 17 153 Cuts at: 6715 6900 7345 325 Size: 185 445 1498 Fragments arranged by size: 2322 1498 1255 1181 767 497 445 198 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2341 3431 4657 Size: 274 80 33 51 1443 364 1090 1226 Cuts at: 4657 5692 6764 8476 96 Size: 1035 1072 1712 138 Fragments arranged by size: 1712 1443 1226 1090 1072 1035 364 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2472 2504 2740 2772 5551 5583 8478 8510 2472 Size: 32 236 32 2779 32 2895 32 2480 Fragments arranged by size: 2895 2779 2480 236 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8518 XbaI T'CTAG_A Cuts at: 3269 5854 3269 Size: 2585 5933 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4340 5277 4340 Size: 937 7581 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8518 XmaI C'CCGG_G Cuts at: 1870 3537 5843 1870 Size: 1667 2306 4545 Fragments arranged by size: 4545 2306 1667 XmnI GAAnn'nnTTC Cuts at: 2021 3211 6647 2021 Size: 1190 3436 3892 Fragments arranged by size: 3892 3436 1190 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6326 276 Size: 53 83 186 936 4792 2468 Fragments arranged by size: 4792 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstXI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI