(Circular) (Six-base) MAPSORT of: pFN21AA1090.seq Check: 4910 from: 1 to: 8800 pFN21AA1090 8800 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4130 5125 4130 Size: 995 7805 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6610 278 Size: 53 83 186 936 5074 2468 Fragments arranged by size: 5074 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6143 1937 Size: 4206 4594 Acc65I G'GTAC_C Cuts at: 6121 6121 Size: 8800 AclI AA'CG_TT Cuts at: 1563 2667 6927 7300 1563 Size: 1104 4260 373 3063 Fragments arranged by size: 4260 3063 1104 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2574 3423 3567 3642 3813 3815 3972 4910 Size: 942 849 144 75 171 2 157 938 Cuts at: 4910 4920 5926 6863 8247 8515 1632 Size: 10 1006 937 1384 268 1917 Fragments arranged by size: 1917 1384 1006 942 938 937 849 268 171 157 144 75 10 2 AfeI AGC'GCT Cuts at: 4190 4190 Size: 8800 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8603 AflIII A'CryG_T Cuts at: 1203 5862 7699 8676 1203 Size: 4659 1837 977 1327 Fragments arranged by size: 4659 1837 1327 977 AgeI A'CCGG_T Cuts at: 7631 7631 Size: 8800 AhdI GACnn_n'nnGTC Cuts at: 5962 7529 5962 Size: 1567 7233 AleI CACnn'nnGTG Cuts at: 1166 4293 5483 1166 Size: 3127 1190 4483 Fragments arranged by size: 4483 3127 1190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3450 4058 4426 4714 1184 Size: 2266 608 368 288 5270 Fragments arranged by size: 5270 2266 608 368 288 AlwNI CAG_nnn'CTG Cuts at: 3290 6543 8115 3290 Size: 3253 1572 3975 Fragments arranged by size: 3975 3253 1572 ApaI G_GGCC'C Cuts at: 2965 2965 Size: 8800 ApaLI G'TGCA_C Cuts at: 2778 6857 8013 2778 Size: 4079 1156 3565 Fragments arranged by size: 4079 3565 1156 ApoI r'AATT_y Cuts at: 1493 2009 4214 4754 5264 6109 6335 6487 8596 Size: 516 2205 540 510 845 226 152 2109 Cuts at: 8596 1493 Size: 1697 Fragments arranged by size: 2205 2109 1697 845 540 516 510 226 152 AseI AT'TA_AT Cuts at: 160 1032 7354 160 Size: 872 6322 1606 Fragments arranged by size: 6322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8800 AvaI C'yCGr_G Cuts at: 1870 1943 2256 3898 4938 5609 6125 1870 Size: 73 313 1642 1040 671 516 4545 Fragments arranged by size: 4545 1642 1040 671 516 313 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2771 2804 3939 3972 2771 Size: 33 1135 33 7599 Fragments arranged by size: 7599 1135 33 33 BamHI G'GATC_C Cuts at: 6130 6497 6130 Size: 367 8433 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2074 2905 6121 7576 618 Size: 325 217 627 287 831 3216 1455 1842 Fragments arranged by size: 3216 1842 1455 831 627 325 287 217 BanII G_rGCy'C Cuts at: 1391 2965 3912 6119 1391 Size: 1574 947 2207 4072 Fragments arranged by size: 4072 2207 1574 947 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2111 3338 3382 3558 3662 4867 928 Size: 971 212 1227 44 176 104 1205 4861 Fragments arranged by size: 4861 1227 1205 971 212 176 104 44 BbvCI CC'TCA_GC Cuts at: 2147 3627 2147 Size: 1480 7320 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3653 3687 3723 3757 6991 7025 1903 Size: 34 1716 34 36 34 3234 34 3678 Fragments arranged by size: 3678 3234 1716 36 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3591 6692 7902 3591 Size: 3101 1210 4489 Fragments arranged by size: 4489 3101 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8800 BfrBI ATG'CAT Cuts at: 3782 3782 Size: 8800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2903 3089 3530 Size: 107 122 71 631 605 1231 186 441 Cuts at: 3530 3731 7411 136 Size: 201 3680 1525 Fragments arranged by size: 3680 1525 1231 631 605 441 201 186 122 107 71 BglII A'GATC_T Cuts at: 2245 2577 5016 8795 2245 Size: 332 2439 3779 2250 Fragments arranged by size: 3779 2439 2250 332 BlpI GC'TnA_GC Cuts at: 3423 6217 3423 Size: 2794 6006 Bme1580I G_kGCm'C Cuts at: 1790 2782 2965 6861 8017 1790 Size: 992 183 3896 1156 2573 Fragments arranged by size: 3896 2573 1156 992 183 BmgBI CAC'GTC Cuts at: 1318 4489 5863 1318 Size: 3171 1374 4255 Fragments arranged by size: 4255 3171 1374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2959 3373 6531 Size: 416 53 83 399 24 1542 414 3158 Cuts at: 6531 7489 442 Size: 958 1753 Fragments arranged by size: 3158 1753 1542 958 416 414 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8800 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3288 3754 4153 3288 Size: 466 399 7935 Fragments arranged by size: 7935 466 399 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2149 2352 2561 3260 7460 1239 Size: 910 203 209 699 4200 2579 Fragments arranged by size: 4200 2579 910 699 209 203 Bpu10I CC'TnA_GC Cuts at: 2147 3464 3627 2147 Size: 1317 163 7320 Fragments arranged by size: 7320 1317 163 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2566 3060 3731 5441 6925 7790 8088 2566 Size: 494 671 1710 1484 865 298 3278 Fragments arranged by size: 3278 1710 1484 865 671 494 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3227 3318 3892 5189 5444 7463 882 Size: 617 1728 91 574 1297 255 2019 2219 Fragments arranged by size: 2219 2019 1728 1297 617 574 255 91 BsaAI yAC'GTr Cuts at: 493 1204 2794 6591 493 Size: 711 1590 3797 2702 Fragments arranged by size: 3797 2702 1590 711 BsaBI GATnn'nnATC Cuts at: 5073 8794 5073 Size: 3721 5079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2211 2811 6607 6989 Size: 53 83 186 936 678 600 3796 382 Cuts at: 6989 7624 275 Size: 635 1451 Fragments arranged by size: 3796 1451 936 678 635 600 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7233 7631 7905 8052 8463 7233 Size: 398 274 147 411 7570 Fragments arranged by size: 7570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3995 4025 5253 5283 1178 Size: 30 165 30 2592 30 1228 30 4695 Fragments arranged by size: 4695 2592 1228 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2530 3032 3261 3306 3318 3461 3730 3999 Size: 1344 502 229 45 12 143 269 269 Cuts at: 3999 5315 5318 1186 Size: 1316 3 4668 Fragments arranged by size: 4668 1344 1316 502 269 269 229 143 45 12 3 BseYI C'CCAG_C Cuts at: 2157 4552 4625 8003 8499 2157 Size: 2395 73 3378 496 2458 Fragments arranged by size: 3378 2458 2395 496 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3954 4426 4622 5764 5932 1163 Size: 573 2218 472 196 1142 168 4031 Fragments arranged by size: 4031 2218 1142 573 472 196 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6250 7011 8039 1540 Size: 275 186 4249 761 1028 2301 Fragments arranged by size: 4249 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2782 3429 3505 4517 5925 6861 6946 8017 2782 Size: 647 76 1012 1408 936 85 1071 3565 Fragments arranged by size: 3565 1408 1071 1012 936 647 85 76 BsmI GAATG_Cn' Cuts at: 3186 6311 6404 3186 Size: 3125 93 5582 Fragments arranged by size: 5582 3125 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3897 3897 Size: 8800 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2782 2965 3429 3505 3912 4517 5925 Size: 399 992 183 464 76 407 605 1408 Cuts at: 5925 6119 6861 6946 8017 1391 Size: 194 742 85 1071 2174 Fragments arranged by size: 2174 1408 1071 992 742 605 464 407 399 194 183 85 76 BspEI T'CCGG_A Cuts at: 8463 8463 Size: 8800 BspHI T'CATG_A Cuts at: 6689 8419 6689 Size: 1730 7070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4130 5125 6155 844 Size: 3286 995 1030 3489 Fragments arranged by size: 3489 3286 1030 995 BsrBI CCG'CTC Cuts at: 1394 1823 3022 6687 1394 Size: 429 1199 3665 3507 Fragments arranged by size: 3665 3507 1199 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3096 4618 5566 7295 7469 65 Size: 1183 1848 1522 948 1729 174 1396 Fragments arranged by size: 1848 1729 1522 1396 1183 948 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4687 5577 7444 7631 1700 Size: 110 2877 890 1867 187 2869 Fragments arranged by size: 2877 2869 1867 890 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8800 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8800 BssSI C'ACGA_G Cuts at: 4742 5333 5481 6860 7872 4742 Size: 591 148 1379 1012 5670 Fragments arranged by size: 5670 1379 1012 591 148 BstAPI GCAn_nnn'nTGC Cuts at: 794 2288 4440 4617 5758 5778 794 Size: 1494 2152 177 1141 20 3816 Fragments arranged by size: 3816 2152 1494 1141 177 20 BstBI TT'CG_AA Cuts at: 7604 7604 Size: 8800 BstEII G'GTnAC_C Cuts at: 1184 2699 1184 Size: 1515 7285 BstYI r'GATC_y Cuts at: 1972 2245 2577 2731 5016 6130 6497 6882 6899 Size: 273 332 154 2285 1114 367 385 17 Cuts at: 6899 8351 8437 8449 8795 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2285 1977 1452 1114 385 367 346 332 273 154 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3133 4138 3133 Size: 1005 7795 BtgI C'CryG_G Cuts at: 513 1065 2004 3726 3816 5145 513 Size: 552 939 1722 90 1329 4168 Fragments arranged by size: 4168 1722 1329 939 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8061 Fragments arranged by size: 8061 683 56 BtsI GCAGTG_nn' Cuts at: 2098 4443 4455 4886 5605 6316 7110 7130 2098 Size: 2345 12 431 719 711 794 20 3768 Fragments arranged by size: 3768 2345 794 719 711 431 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5581 5616 529 Size: 35 5017 35 3713 Fragments arranged by size: 5017 3713 35 35 DraI TTT'AAA Cuts at: 6104 6104 Size: 8800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7807 809 Size: 507 6491 1802 Fragments arranged by size: 6491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2203 3702 4685 Size: 54 1425 50 275 18 373 1499 983 Cuts at: 4685 6247 7136 8765 8 Size: 1562 889 1629 43 Fragments arranged by size: 1629 1562 1499 1425 983 889 373 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6247 1537 Size: 275 4435 4090 Fragments arranged by size: 4435 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2507 2632 2834 3174 3191 3257 3295 3450 Size: 1154 125 202 340 17 66 38 155 Cuts at: 3450 3671 4864 5702 6050 6730 1353 Size: 221 1193 838 348 680 3423 Fragments arranged by size: 3423 1193 1154 838 680 348 340 221 202 155 125 66 38 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3220 6082 6094 7387 7761 7907 Size: 1064 138 1631 2862 12 1293 374 146 Cuts at: 7907 387 Size: 1280 Fragments arranged by size: 2862 1631 1293 1280 1064 374 146 138 12 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2149 2352 2561 2574 3260 3423 3567 Size: 393 517 203 209 13 686 163 144 Cuts at: 3567 3642 3813 3815 3972 4910 4920 5926 6863 Size: 75 171 2 157 938 10 1006 937 Cuts at: 6863 7460 8247 8515 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1006 938 937 787 686 597 517 393 268 209 203 171 163 157 144 75 13 10 2 EcoNI CCTnn'n_nnAGG Cuts at: 2177 2177 Size: 8800 EcoO109I rG'GnC_Cy Cuts at: 2548 2961 3604 4055 5230 2548 Size: 413 643 451 1175 6118 Fragments arranged by size: 6118 1175 643 451 413 EcoRI G'AATT_C Cuts at: 4754 6109 6487 4754 Size: 1355 378 7067 Fragments arranged by size: 7067 1355 378 EcoRV GAT'ATC Cuts at: 4651 4651 Size: 8800 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2434 2582 3055 4657 5842 2434 Size: 148 473 1602 1185 5392 Fragments arranged by size: 5392 1602 1185 473 148 FspI TGC'GCA Cuts at: 7306 7306 Size: 8800 HaeII r_GCGC'y Cuts at: 1678 4192 5185 7947 1678 Size: 2514 993 2762 2531 Fragments arranged by size: 2762 2531 2514 993 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2569 2601 3359 3391 3672 Size: 32 135 32 997 32 758 32 281 Cuts at: 3672 3704 3896 3928 5948 5980 7441 7473 7515 Size: 32 192 32 2020 32 1461 32 42 Cuts at: 7515 7547 1373 Size: 32 2626 Fragments arranged by size: 2626 2020 1461 997 758 281 192 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4492 5272 6144 6390 669 Size: 988 281 2554 780 872 246 3079 Fragments arranged by size: 3079 2554 988 872 780 281 246 HindIII A'AGCT_T Cuts at: 748 4922 748 Size: 4174 4626 HpaI GTT'AAC Cuts at: 5272 6390 5272 Size: 1118 7682 KpnI G_GTAC'C Cuts at: 6125 6125 Size: 8800 MfeI C'AATT_G Cuts at: 5593 6399 5593 Size: 806 7994 MluI A'CGCG_T Cuts at: 8676 8676 Size: 8800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4338 4452 7914 8098 1305 Size: 3033 114 3462 184 2007 Fragments arranged by size: 3462 3033 2007 184 114 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6978 Fragments arranged by size: 6978 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2063 3011 3191 3408 3432 4293 4586 Size: 648 897 948 180 217 24 861 293 Cuts at: 4586 4658 4988 5483 5856 6758 7117 7276 518 Size: 72 330 495 373 902 359 159 2042 Fragments arranged by size: 2042 948 902 897 861 648 495 373 359 330 293 217 180 159 72 24 MspA1I CmG'CkG Cuts at: 1606 4018 4278 4293 4365 4386 4556 4602 5027 Size: 2412 260 15 72 21 170 46 425 Cuts at: 5027 5147 5433 5736 5755 5946 6216 6893 8041 Size: 120 286 303 19 191 270 677 1148 Cuts at: 8041 8286 1606 Size: 245 2120 Fragments arranged by size: 2412 2120 1148 677 425 303 286 270 260 245 191 170 120 72 46 21 19 15 NaeI GCC'GGC Cuts at: 4689 4689 Size: 8800 NcoI C'CATG_G Cuts at: 513 1065 2004 3726 513 Size: 552 939 1722 5587 Fragments arranged by size: 5587 1722 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8800 NgoMIV G'CCGG_C Cuts at: 4687 4687 Size: 8800 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8800 NotI GC'GGCC_GC Cuts at: 6247 6247 Size: 8800 NsiI A_TGCA'T Cuts at: 3784 3784 Size: 8800 NspI r_CATG'y Cuts at: 1132 1229 3010 3786 5362 6158 7703 1132 Size: 97 1781 776 1576 796 1545 2229 Fragments arranged by size: 2229 1781 1576 1545 796 776 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8800 PciI A'CATG_T Cuts at: 7699 7699 Size: 8800 PflMI CCAn_nnn'nTGG Cuts at: 1108 2633 3385 1108 Size: 1525 752 6523 Fragments arranged by size: 6523 1525 752 PfoI T'CCnGG_A Cuts at: 4097 4644 4097 Size: 547 8253 PmeI GTTT'AAAC Cuts at: 6104 6104 Size: 8800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6869 6901 8382 8414 6869 Size: 32 1481 32 7255 Fragments arranged by size: 7255 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3604 4055 3604 Size: 451 8349 PshAI GACnn'nnGTC Cuts at: 1613 3338 1613 Size: 1725 7075 PsiI TTA'TAA Cuts at: 6370 6370 Size: 8800 PspOMI G'GGCC_C Cuts at: 2961 2961 Size: 8800 PstI C_TGCA'G Cuts at: 830 2178 2731 3106 3232 4127 4622 5518 6152 Size: 1348 553 375 126 895 495 896 634 Cuts at: 6152 830 Size: 3478 Fragments arranged by size: 3478 1348 896 895 634 553 495 375 126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8800 PvuII CAG'CTG Cuts at: 4018 4293 4602 5433 5755 5946 4018 Size: 275 309 831 322 191 6872 Fragments arranged by size: 6872 831 322 309 275 191 SacII CC_GC'GG Cuts at: 5148 5148 Size: 8800 SalI G'TCGA_C Cuts at: 1936 6142 1936 Size: 4206 4594 SapI GCTCTTCn'nnn_ Cuts at: 2834 3450 2834 Size: 616 8184 SbfI CC_TGCA'GG Cuts at: 2178 5518 6152 2178 Size: 3340 634 4826 Fragments arranged by size: 4826 3340 634 ScaI AGT'ACT Cuts at: 3268 7048 8760 3268 Size: 3780 1712 3308 Fragments arranged by size: 3780 3308 1712 SfcI C'TryA_G Cuts at: 826 1045 2174 2727 3102 3228 3609 4123 4618 Size: 219 1129 553 375 126 381 514 495 Cuts at: 4618 4675 5367 5514 6148 7283 7964 8155 826 Size: 57 692 147 634 1135 681 191 1471 Fragments arranged by size: 1471 1135 1129 692 681 634 553 514 495 381 375 219 191 147 126 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8800 SmaI CCC'GGG Cuts at: 1872 2258 3900 6127 1872 Size: 386 1642 2227 4545 Fragments arranged by size: 4545 2227 1642 386 SmlI C'TyrA_G Cuts at: 820 1017 1943 2581 3075 3746 5456 6904 7805 Size: 197 926 638 494 671 1710 1448 901 Cuts at: 7805 8067 820 Size: 262 1553 Fragments arranged by size: 1710 1553 1448 926 901 671 638 494 262 197 SnaBI TAC'GTA Cuts at: 493 6591 493 Size: 6098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8800 SphI G_CATG'C Cuts at: 1132 6158 1132 Size: 5026 3774 SspI AAT'ATT Cuts at: 5 52 4726 6724 5 Size: 47 4674 1998 2081 Fragments arranged by size: 4674 2081 1998 47 StuI AGG'CCT Cuts at: 1517 5789 1517 Size: 4272 4528 StyI C'CwwG_G Cuts at: 513 1065 2004 2705 3084 3726 4087 4328 5784 Size: 552 939 701 379 642 361 241 1456 Cuts at: 5784 5812 5846 6239 513 Size: 28 34 393 3074 Fragments arranged by size: 3074 1456 939 701 642 552 393 379 361 241 34 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2923 3444 3652 4648 5363 5989 6827 Size: 1255 1343 521 208 996 715 626 838 Cuts at: 6827 6844 6997 7182 7627 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1343 1255 996 838 715 626 521 445 208 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3266 7046 8758 Size: 274 80 33 51 1443 1289 3780 1712 Cuts at: 8758 96 Size: 138 Fragments arranged by size: 3780 1712 1443 1289 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2067 2099 3995 4027 4401 4433 8760 8792 2067 Size: 32 1896 32 374 32 4327 32 2075 Fragments arranged by size: 4327 2075 1896 374 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8800 XbaI T'CTAG_A Cuts at: 6136 6136 Size: 8800 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2823 3523 2823 Size: 700 8100 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8800 XmaI C'CCGG_G Cuts at: 1870 2256 3898 6125 1870 Size: 386 1642 2227 4545 Fragments arranged by size: 4545 2227 1642 386 XmnI GAAnn'nnTTC Cuts at: 6929 6929 Size: 8800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6608 276 Size: 53 83 186 936 5074 2468 Fragments arranged by size: 5074 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BsiWI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI