(Circular) (Six-base) MAPSORT of: pFN21AA1071.seq Check: 1515 from: 1 to: 7957 pFN21AA1071 7957 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3057 3057 Size: 7957 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5767 278 Size: 53 83 186 936 4231 2468 Fragments arranged by size: 4231 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5300 1937 Size: 3363 4594 Acc65I G'GTAC_C Cuts at: 5278 5278 Size: 7957 AclI AA'CG_TT Cuts at: 1563 3957 6084 6457 1563 Size: 2394 2127 373 3063 Fragments arranged by size: 3063 2394 2127 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2037 2714 5004 6020 7404 7672 1632 Size: 405 677 2290 1016 1384 268 1917 Fragments arranged by size: 2290 1917 1384 1016 677 405 268 AflII C'TTAA_G Cuts at: 820 1017 2501 820 Size: 197 1484 6276 Fragments arranged by size: 6276 1484 197 AflIII A'CryG_T Cuts at: 1203 6856 7833 1203 Size: 5653 977 1327 Fragments arranged by size: 5653 1327 977 AgeI A'CCGG_T Cuts at: 5102 6788 5102 Size: 1686 6271 AhdI GACnn_n'nnGTC Cuts at: 6686 6686 Size: 7957 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7957 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3642 1184 Size: 2458 5499 AlwNI CAG_nnn'CTG Cuts at: 3739 4667 4745 4772 4796 4832 4853 4874 5215 Size: 928 78 27 24 36 21 21 341 Cuts at: 5215 5700 7272 3739 Size: 485 1572 4424 Fragments arranged by size: 4424 1572 928 485 341 78 36 27 24 21 21 ApaI G_GGCC'C Cuts at: 2174 2174 Size: 7957 ApaLI G'TGCA_C Cuts at: 6014 7170 6014 Size: 1156 6801 ApoI r'AATT_y Cuts at: 1493 2011 2651 5266 5492 5644 7753 1493 Size: 518 640 2615 226 152 2109 1697 Fragments arranged by size: 2615 2109 1697 640 518 226 152 AseI AT'TA_AT Cuts at: 160 1032 6511 160 Size: 872 5479 1606 Fragments arranged by size: 5479 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7957 AvaI C'yCGr_G Cuts at: 1870 1943 2167 3237 5282 1870 Size: 73 224 1070 2045 4545 Fragments arranged by size: 4545 2045 1070 224 73 AvrII C'CTAG_G Cuts at: 3053 4474 3053 Size: 1421 6536 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4576 4609 4576 Size: 33 7924 BamHI G'GATC_C Cuts at: 5287 5654 5287 Size: 367 7590 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5011 5278 6733 618 Size: 325 217 627 3224 267 1455 1842 Fragments arranged by size: 3224 1842 1455 627 325 267 217 BanII G_rGCy'C Cuts at: 1391 2174 3244 3307 3993 5276 1391 Size: 783 1070 63 686 1283 4072 Fragments arranged by size: 4072 1283 1070 783 686 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6986 BbvCI CC'TCA_GC Cuts at: 4867 5079 4867 Size: 212 7745 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4534 4568 6148 6182 1903 Size: 34 2597 34 1580 34 3678 Fragments arranged by size: 3678 2597 1580 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5849 7059 5849 Size: 1210 6747 BclI T'GATC_A Cuts at: 1548 4287 1548 Size: 2739 5218 BfrBI ATG'CAT Cuts at: 3517 3517 Size: 7957 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2697 6568 136 Size: 107 122 71 631 605 1025 3871 1525 Fragments arranged by size: 3871 1525 1025 631 605 122 107 71 BglII A'GATC_T Cuts at: 7952 7952 Size: 7957 BlpI GC'TnA_GC Cuts at: 3243 5374 3243 Size: 2131 5826 Bme1580I G_kGCm'C Cuts at: 1790 2174 2787 6018 7174 1790 Size: 384 613 3231 1156 2573 Fragments arranged by size: 3231 2573 1156 613 384 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7957 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2145 2347 5688 Size: 416 53 83 399 24 728 202 3341 Cuts at: 5688 6646 442 Size: 958 1753 Fragments arranged by size: 3341 1753 958 728 416 399 202 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7957 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3343 3343 Size: 7957 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2427 3827 3917 4283 4644 4722 4749 4773 Size: 1188 1400 90 366 361 78 27 24 Cuts at: 4773 4809 4905 4920 4956 5028 5046 6617 1239 Size: 36 96 15 36 72 18 1571 2579 Fragments arranged by size: 2579 1571 1400 1188 366 361 96 90 78 72 36 36 27 24 18 15 Bpu10I CC'TnA_GC Cuts at: 4867 4983 5073 5079 4867 Size: 116 90 6 7745 Fragments arranged by size: 7745 116 90 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2545 3367 6082 6947 7245 2545 Size: 822 2715 865 298 3257 Fragments arranged by size: 3257 2715 865 822 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2490 3027 6620 882 Size: 617 991 537 3593 2219 Fragments arranged by size: 3593 2219 991 617 537 BsaAI yAC'GTr Cuts at: 493 1204 5748 493 Size: 711 4544 2702 Fragments arranged by size: 4544 2702 711 BsaBI GATnn'nnATC Cuts at: 7951 7951 Size: 7957 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2440 5764 6146 6781 Size: 53 83 186 936 907 3324 382 635 Cuts at: 6781 275 Size: 1451 Fragments arranged by size: 3324 1451 936 907 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3339 3885 4960 5102 6390 6788 7062 7209 7620 Size: 546 1075 142 1288 398 274 147 411 Cuts at: 7620 3339 Size: 3676 Fragments arranged by size: 3676 1288 1075 546 411 398 274 147 142 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2578 2608 3095 3125 5008 Size: 30 165 30 1175 30 487 30 1883 Cuts at: 5008 5032 5038 5044 5062 5074 1178 Size: 24 6 6 18 12 4061 Fragments arranged by size: 4061 1883 1175 487 165 30 30 30 30 24 18 12 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2286 4022 4079 4208 1186 Size: 1100 1736 57 129 4935 Fragments arranged by size: 4935 1736 1100 129 57 BseYI C'CCAG_C Cuts at: 3306 7160 7656 3306 Size: 3854 496 3607 Fragments arranged by size: 3854 3607 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3963 1163 Size: 573 2227 5157 Fragments arranged by size: 5157 2227 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5407 6168 7196 1540 Size: 275 186 3406 761 1028 2301 Fragments arranged by size: 3406 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2596 3879 3993 6018 6103 7174 2596 Size: 1283 114 2025 85 1071 3379 Fragments arranged by size: 3379 2025 1283 1071 114 85 BsmI GAATG_Cn' Cuts at: 2307 4479 5468 5561 2307 Size: 2172 989 93 4703 Fragments arranged by size: 4703 2172 989 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3113 3424 3887 4443 4791 3113 Size: 311 463 556 348 6279 Fragments arranged by size: 6279 556 463 348 311 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 2596 2787 3244 3307 3879 3993 Size: 399 384 422 191 457 63 572 114 Cuts at: 3993 5276 6018 6103 7174 1391 Size: 1283 742 85 1071 2174 Fragments arranged by size: 2174 1283 1071 742 572 457 422 399 384 191 114 85 63 BspEI T'CCGG_A Cuts at: 3885 7620 3885 Size: 3735 4222 BspHI T'CATG_A Cuts at: 5846 7576 5846 Size: 1730 6227 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3057 5150 5312 844 Size: 2213 2093 162 3489 Fragments arranged by size: 3489 2213 2093 162 BsrBI CCG'CTC Cuts at: 1394 1823 5844 1394 Size: 429 4021 3507 Fragments arranged by size: 4021 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2894 6452 6626 65 Size: 1183 1646 3558 174 1396 Fragments arranged by size: 3558 1646 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5102 6601 6788 1700 Size: 110 3292 1499 187 2869 Fragments arranged by size: 3292 2869 1499 187 110 BsrGI T'GTAC_A Cuts at: 96 3835 96 Size: 3739 4218 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7957 BssSI C'ACGA_G Cuts at: 3440 6017 7029 3440 Size: 2577 1012 4368 Fragments arranged by size: 4368 2577 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2056 4637 794 Size: 1262 2581 4114 Fragments arranged by size: 4114 2581 1262 BstBI TT'CG_AA Cuts at: 6761 6761 Size: 7957 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7957 BstXI CCAn_nnnn'nTGG Cuts at: 4723 5188 4723 Size: 465 7492 BstYI r'GATC_y Cuts at: 1972 3321 3532 5211 5287 5654 6039 6056 7508 Size: 1349 211 1679 76 367 385 17 1452 Cuts at: 7508 7594 7606 7952 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1679 1452 1349 385 367 346 211 86 76 17 12 BtgI C'CryG_G Cuts at: 513 1065 2040 4722 513 Size: 552 975 2682 3748 Fragments arranged by size: 3748 2682 975 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2786 487 Size: 683 56 1560 5658 Fragments arranged by size: 5658 1560 683 56 BtsI GCAGTG_nn' Cuts at: 2164 3681 4677 4692 4698 4794 5473 6267 6287 Size: 1517 996 15 6 96 679 794 20 Cuts at: 6287 2164 Size: 3834 Fragments arranged by size: 3834 1517 996 794 679 96 20 15 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7957 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5103 5138 529 Size: 35 4539 35 3348 Fragments arranged by size: 4539 3348 35 35 DraI TTT'AAA Cuts at: 5261 5261 Size: 7957 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4130 6964 809 Size: 507 2814 2834 1802 Fragments arranged by size: 2834 2814 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2553 3678 4212 Size: 54 1425 50 275 18 723 1125 534 Cuts at: 4212 4352 5404 6293 7922 8 Size: 140 1052 889 1629 43 Fragments arranged by size: 1629 1425 1125 1052 889 723 534 275 140 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5404 1537 Size: 275 3592 4090 Fragments arranged by size: 4090 3592 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3771 5887 1353 Size: 2418 2116 3423 Fragments arranged by size: 3423 2418 2116 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6544 6918 7064 387 Size: 1064 138 4955 374 146 1280 Fragments arranged by size: 4955 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3991 3991 Size: 7957 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2427 2714 3827 3917 4283 4644 Size: 393 405 390 287 1113 90 366 361 Cuts at: 4644 4722 4749 4773 4809 4905 4920 4956 5004 Size: 78 27 24 36 96 15 36 48 Cuts at: 5004 5028 5046 6020 6617 7404 7672 1239 Size: 24 18 974 597 787 268 1524 Fragments arranged by size: 1524 1113 974 787 597 405 393 390 366 361 287 268 96 90 78 48 36 36 27 24 24 18 15 EcoO109I rG'GnC_Cy Cuts at: 2171 2714 2958 3466 3736 3829 2171 Size: 543 244 508 270 93 6299 Fragments arranged by size: 6299 543 508 270 244 93 EcoRI G'AATT_C Cuts at: 2651 5266 5644 2651 Size: 2615 378 4964 Fragments arranged by size: 4964 2615 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2087 2690 2087 Size: 603 7354 FspI TGC'GCA Cuts at: 6463 6463 Size: 7957 HaeII r_GCGC'y Cuts at: 1678 7104 1678 Size: 5426 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2830 2862 3529 3561 3702 Size: 32 135 32 1258 32 667 32 141 Cuts at: 3702 3734 4006 4038 4325 4357 5131 5163 6598 Size: 32 272 32 287 32 774 32 1435 Cuts at: 6598 6630 6672 6704 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1435 1258 774 667 287 272 141 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3805 5301 5547 669 Size: 988 281 1867 1496 246 3079 Fragments arranged by size: 3079 1867 1496 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7957 HpaI GTT'AAC Cuts at: 5547 5547 Size: 7957 KpnI G_GTAC'C Cuts at: 5282 5282 Size: 7957 MfeI C'AATT_G Cuts at: 5556 5556 Size: 7957 MluI A'CGCG_T Cuts at: 7833 7833 Size: 7957 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2365 3339 5063 5075 5081 7071 7255 1305 Size: 1060 974 1724 12 6 1990 184 2007 Fragments arranged by size: 2007 1990 1724 1060 974 184 12 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 2555 3680 4214 10 Size: 54 1425 343 723 1125 534 3753 Fragments arranged by size: 3753 1425 1125 723 534 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2379 2481 4721 5915 6274 6433 518 Size: 648 1213 102 2240 1194 359 159 2042 Fragments arranged by size: 2240 2042 1213 1194 648 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2290 2455 4339 4634 4769 4802 4829 4850 Size: 684 165 1884 295 135 33 27 21 Cuts at: 4850 4871 4925 4979 5066 5373 6050 7198 7443 Size: 21 54 54 87 307 677 1148 245 Cuts at: 7443 1606 Size: 2120 Fragments arranged by size: 2120 1884 1148 684 677 307 295 245 165 135 87 54 54 33 27 21 21 NcoI C'CATG_G Cuts at: 513 1065 4722 513 Size: 552 3657 3748 Fragments arranged by size: 3748 3657 552 NdeI CA'TA_TG Cuts at: 387 3430 387 Size: 3043 4914 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7957 NotI GC'GGCC_GC Cuts at: 5404 5404 Size: 7957 NsiI A_TGCA'T Cuts at: 3519 3519 Size: 7957 NspI r_CATG'y Cuts at: 1132 1229 2378 2516 2746 3855 4351 5315 6860 Size: 97 1149 138 230 1109 496 964 1545 Cuts at: 6860 1132 Size: 2229 Fragments arranged by size: 2229 1545 1149 1109 964 496 230 138 97 PasI CC'CwG_GG Cuts at: 1348 2245 1348 Size: 897 7060 PciI A'CATG_T Cuts at: 6856 6856 Size: 7957 PflMI CCAn_nnn'nTGG Cuts at: 1108 3313 1108 Size: 2205 5752 PfoI T'CCnGG_A Cuts at: 2464 4310 5107 2464 Size: 1846 797 5314 Fragments arranged by size: 5314 1846 797 PmeI GTTT'AAAC Cuts at: 5261 5261 Size: 7957 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6026 6058 7539 7571 6026 Size: 32 1481 32 6412 Fragments arranged by size: 6412 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2958 2958 Size: 7957 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7957 PsiI TTA'TAA Cuts at: 5527 5527 Size: 7957 PspOMI G'GGCC_C Cuts at: 2170 2170 Size: 7957 PstI C_TGCA'G Cuts at: 830 3393 4592 4633 5309 830 Size: 2563 1199 41 676 3478 Fragments arranged by size: 3478 2563 1199 676 41 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7957 PvuII CAG'CTG Cuts at: 2290 4339 4634 4769 4829 4850 4871 4925 5066 Size: 2049 295 135 60 21 21 54 141 Cuts at: 5066 2290 Size: 5181 Fragments arranged by size: 5181 2049 295 141 135 60 54 21 21 SacI G_AGCT'C Cuts at: 3993 3993 Size: 7957 SalI G'TCGA_C Cuts at: 1936 5299 1936 Size: 3363 4594 SapI GCTCTTCn'nnn_ Cuts at: 3771 3771 Size: 7957 SbfI CC_TGCA'GG Cuts at: 5309 5309 Size: 7957 ScaI AGT'ACT Cuts at: 2357 4296 6205 7917 2357 Size: 1939 1909 1712 2397 Fragments arranged by size: 2397 1939 1909 1712 SfcI C'TryA_G Cuts at: 826 1045 2757 2791 3389 4588 4629 5305 6440 Size: 219 1712 34 598 1199 41 676 1135 Cuts at: 6440 7121 7312 826 Size: 681 191 1471 Fragments arranged by size: 1712 1471 1199 1135 681 676 598 219 191 41 34 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7957 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7957 SmaI CCC'GGG Cuts at: 1872 2169 5284 1872 Size: 297 3115 4545 Fragments arranged by size: 4545 3115 297 SmlI C'TyrA_G Cuts at: 820 1017 1943 2501 2524 3346 6061 6962 7224 Size: 197 926 558 23 822 2715 901 262 Cuts at: 7224 820 Size: 1553 Fragments arranged by size: 2715 1553 926 901 822 558 262 197 23 SnaBI TAC'GTA Cuts at: 493 5748 493 Size: 5255 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7957 SphI G_CATG'C Cuts at: 1132 2378 2746 4351 5315 1132 Size: 1246 368 1605 964 3774 Fragments arranged by size: 3774 1605 1246 964 368 SspI AAT'ATT Cuts at: 5 52 4058 5881 5 Size: 47 4006 1823 2081 Fragments arranged by size: 4006 2081 1823 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7957 StyI C'CwwG_G Cuts at: 513 1065 2340 2665 3015 3053 3756 4474 4722 Size: 552 1275 325 350 38 703 718 248 Cuts at: 4722 5396 513 Size: 674 3074 Fragments arranged by size: 3074 1275 718 703 674 552 350 325 248 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4478 5984 6001 6154 6339 6784 325 Size: 1255 2898 1506 17 153 185 445 1498 Fragments arranged by size: 2898 1506 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2355 3835 4294 Size: 274 80 33 51 1443 378 1480 459 Cuts at: 4294 6203 7915 96 Size: 1909 1712 138 Fragments arranged by size: 1909 1712 1480 1443 459 378 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7917 7949 7917 Size: 32 7925 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7957 XbaI T'CTAG_A Cuts at: 3547 4171 5293 3547 Size: 624 1122 6211 Fragments arranged by size: 6211 1122 624 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4604 5212 4604 Size: 608 7349 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7957 XmaI C'CCGG_G Cuts at: 1870 2167 5282 1870 Size: 297 3115 4545 Fragments arranged by size: 4545 3115 297 XmnI GAAnn'nnTTC Cuts at: 6086 6086 Size: 7957 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5765 276 Size: 53 83 186 936 4231 2468 Fragments arranged by size: 4231 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI