Restriction map for
pFN21AA1047
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AscI AseI AsnI AviII
AvrII BbeI BfrI BlnI BseAI
BsiWI BspDI BspEI BspLU11I BsrFI
BssHII BstUI Cfr10I ClaI CpoI
DraIII EcoRI FseI FspI HincII
HindII HpaI KasI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SfiI
SfoI SgrAI SnaBI SphI Sse8387I
SwaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 3448 GGTACC G^GTACC
AcyI 6786 GACGCC G[GA]^CG[CT]C
AflIII 4391 ACACGT A^C[GA][CT]GT
AhdI 3741 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3403 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2698 AGCGCT AGC^GCT
Asp718I 3448 GGTACC G^GTACC
AspEI 3741 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 4940 TGGCCA TGG^CCA
BbrPI 2691 CACGTG CAC^GTG
BbvCI 408 GCTGAGG CCTCAGC(-5/-2)
BsaAI 2691 CACGTG [CT]AC^GT[GA]
BsaHI 6786 GACGCC G[GA]^CG[CT]C
BsaWI 6156 TCCGGT [AT]^CCGG[AT]
BsiEI 4171 CGGCCG CG[GA][CT]^CG
Bsp1407I 6756 TGTACA T^GTACA
BsrGI 6756 TGTACA T^GTACA
BssSI 2069 CTCGTG CACGAG(-5/-1)
Bst1107I 78 GTATAC GTA^TAC
BstEII 4834 GGTTACC G^GT[ACGT]ACC
BstPI 4834 GGTTACC G^GT[ACGT]ACC
BstXI 4742 CCACGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 78 GTATAC GTA^TAC
Bsu36I 3403 CCTGAGG CC^T[ACGT]AGG
EagI 4171 CGGCCG C^GGCCG
Eam1105I 3741 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4171 CGGCCG C^GGCCG
Eco47III 2698 AGCGCT AGC^GCT
Eco52I 4171 CGGCCG C^GGCCG
Eco81I 3403 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4834 GGTTACC G^GT[ACGT]ACC
EcoRV 5571 GATATC GAT^ATC
HaeII 2698 AGCGCT [GA]GCGC^[CT]
Hin1I 6786 GACGCC G[GA]^CG[CT]C
KpnI 3448 GGTACC GGTAC^C
MluNI 4940 TGGCCA TGG^CCA
MscI 4940 TGGCCA TGG^CCA
NspI 6895 GCATGT [GA]CATG^[CT]
PaeR7I 2276 CTCGAG C^TCGAG
PmaCI 2691 CACGTG CAC^GTG
PmlI 2691 CACGTG CAC^GTG
PshAI 3628 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 6874 AGTACT AGT^ACT
SmaI 1706 CCCGGG CCC^GGG
SpeI 259 ACTAGT A^CTAGT
SspBI 6756 TGTACA T^GTACA
SspI 3718 AATATT AAT^ATT
XbaI 6436 TCTAGA T^CTAGA
XhoI 2276 CTCGAG C^TCGAG
XmaCI 1706 CCCGGG C^CCGGG
XmaI 1706 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 78 GTATAC GT^[AC][GT]AC
4581 GTCTAC
Alw44I 388 GTGCAC G^TGCAC
4487 GTGCAC
ApaI 3422 GGGCCC GGGCC^C
5451 GGGCCC
ApaLI 388 GTGCAC G^TGCAC
4487 GTGCAC
BcgI 186 GCAACAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1509 GCAAATTGCTCG
BciVI 2869 GTATCC GTATCC(6/5)
4631 GTATCC
BglII 1337 AGATCT A^GATCT
6392 AGATCT
BsmBI 2793 CGTCTC CGTCTC(1/5)
6114 CGTCTC
BstBI 194 TTCGAA TT^CGAA
1046 TTCGAA
DraI 877 TTTAAA TTT^AAA
3774 TTTAAA
DrdI 3915 GACTGTATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5288 GACCTGGCAGTC
EcoT22I 2543 ATGCAT ATGCA^T
2947 ATGCAT
HgaI 6786 GACGC GACGC(5/10)
4929 GCGTC
MfeI 1946 CAATTG C^AATTG
2894 CAATTG
MunI 1946 CAATTG C^AATTG
2894 CAATTG
NcoI 5069 CCATGG C^CATGG
6299 CCATGG
NdeI 3710 CATATG CA^TATG
4058 CATATG
NsiI 2543 ATGCAT ATGCA^T
2947 ATGCAT
NspV 194 TTCGAA TT^CGAA
1046 TTCGAA
SapI 1813 GAAGAGC GCTCTTC(1/4)
2074 GAAGAGC
SfuI 194 TTCGAA TT^CGAA
1046 TTCGAA
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# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AspI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflFI PflMI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI