(Circular) (Six-base) MAPSORT of: pFN21AA1031.seq Check: 8552 from: 1 to: 7636 pFN21AA1031 7636 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4108 4108 Size: 7636 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5446 278 Size: 53 83 186 936 3910 2468 Fragments arranged by size: 3910 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2060 3104 4979 1937 Size: 123 1044 1875 4594 Fragments arranged by size: 4594 1875 1044 123 Acc65I G'GTAC_C Cuts at: 4957 4957 Size: 7636 AclI AA'CG_TT Cuts at: 1563 5763 6136 1563 Size: 4200 373 3063 Fragments arranged by size: 4200 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3653 4802 5699 7083 7351 1632 Size: 2021 1149 897 1384 268 1917 Fragments arranged by size: 2021 1917 1384 1149 897 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7439 AflIII A'CryG_T Cuts at: 1203 3534 3849 4359 4413 6535 7512 1203 Size: 2331 315 510 54 2122 977 1327 Fragments arranged by size: 2331 2122 1327 977 510 315 54 AgeI A'CCGG_T Cuts at: 6467 6467 Size: 7636 AhdI GACnn_n'nnGTC Cuts at: 2818 3721 6365 2818 Size: 903 2644 4089 Fragments arranged by size: 4089 2644 903 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7636 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2870 4829 1184 Size: 1686 1959 3991 Fragments arranged by size: 3991 1959 1686 AlwNI CAG_nnn'CTG Cuts at: 2119 2170 2491 4641 5379 6951 2119 Size: 51 321 2150 738 1572 2804 Fragments arranged by size: 2804 2150 1572 738 321 51 ApaI G_GGCC'C Cuts at: 3035 4062 3035 Size: 1027 6609 ApaLI G'TGCA_C Cuts at: 3779 5693 6849 3779 Size: 1914 1156 4566 Fragments arranged by size: 4566 1914 1156 ApoI r'AATT_y Cuts at: 1493 4945 5171 5323 7432 1493 Size: 3452 226 152 2109 1697 Fragments arranged by size: 3452 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6190 160 Size: 872 5158 1606 Fragments arranged by size: 5158 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7636 AvaI C'yCGr_G Cuts at: 1870 1943 2235 2646 3028 3306 4470 4961 1870 Size: 73 292 411 382 278 1164 491 4545 Fragments arranged by size: 4545 1164 491 411 382 292 278 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2870 2903 2870 Size: 33 7603 BamHI G'GATC_C Cuts at: 3408 4966 5333 3408 Size: 1558 367 5711 Fragments arranged by size: 5711 1558 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3557 3707 4886 4957 6412 Size: 325 217 627 1770 150 1179 71 1455 Cuts at: 6412 618 Size: 1842 Fragments arranged by size: 1842 1770 1455 1179 627 325 217 150 71 BanII G_rGCy'C Cuts at: 1391 3035 3210 3386 3446 3924 4062 4752 4955 Size: 1644 175 176 60 478 138 690 203 Cuts at: 4955 1391 Size: 4072 Fragments arranged by size: 4072 1644 690 478 203 176 175 138 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3972 4606 928 Size: 971 2073 634 3958 Fragments arranged by size: 3958 2073 971 634 BbvCI CC'TCA_GC Cuts at: 2127 2127 Size: 7636 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2192 2226 5827 5861 1903 Size: 34 255 34 3601 34 3678 Fragments arranged by size: 3678 3601 255 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2360 5528 6738 2360 Size: 3168 1210 3258 Fragments arranged by size: 3258 3168 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7636 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2844 6247 136 Size: 107 122 71 631 605 1172 3403 1525 Fragments arranged by size: 3403 1525 1172 631 605 122 107 71 BglII A'GATC_T Cuts at: 3669 7631 3669 Size: 3962 3674 BlpI GC'TnA_GC Cuts at: 5053 5053 Size: 7636 Bme1580I G_kGCm'C Cuts at: 1790 3035 3560 3783 4062 4826 5697 6853 1790 Size: 1245 525 223 279 764 871 1156 2573 Fragments arranged by size: 2573 1245 1156 871 764 525 279 223 BmgBI CAC'GTC Cuts at: 1318 3535 3850 4620 1318 Size: 2217 315 770 4334 Fragments arranged by size: 4334 2217 770 315 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2399 2501 4938 Size: 416 53 83 399 24 982 102 2437 Cuts at: 4938 5367 6325 442 Size: 429 958 1753 Fragments arranged by size: 2437 1753 982 958 429 416 399 102 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7636 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3169 3169 Size: 7636 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2330 2926 4126 4467 4673 6296 1239 Size: 1091 596 1200 341 206 1623 2579 Fragments arranged by size: 2579 1623 1200 1091 596 341 206 Bpu10I CC'TnA_GC Cuts at: 2127 2127 Size: 7636 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2087 3359 3788 3908 4127 5761 6626 6924 2087 Size: 1272 429 120 219 1634 865 298 2799 Fragments arranged by size: 2799 1634 1272 865 429 298 219 120 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3065 3140 6299 882 Size: 617 1566 75 3159 2219 Fragments arranged by size: 3159 2219 1566 617 75 BsaAI yAC'GTr Cuts at: 493 1204 2638 5427 493 Size: 711 1434 2789 2702 Fragments arranged by size: 2789 2702 1434 711 BsaBI GATnn'nnATC Cuts at: 7630 7630 Size: 7636 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2395 4632 5443 5825 Size: 53 83 186 936 862 2237 811 382 Cuts at: 5825 6460 275 Size: 635 1451 Fragments arranged by size: 2237 1451 936 862 811 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3378 6069 6467 6741 6888 7299 3378 Size: 2691 398 274 147 411 3715 Fragments arranged by size: 3715 2691 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2231 2261 1178 Size: 30 165 30 828 30 6553 Fragments arranged by size: 6553 828 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3158 3539 3560 4364 4760 1186 Size: 1972 381 21 804 396 4062 Fragments arranged by size: 4062 1972 804 396 381 21 BseYI C'CCAG_C Cuts at: 2329 2656 2744 2871 3589 4061 4641 6839 7335 Size: 327 88 127 718 472 580 2198 496 Cuts at: 7335 2329 Size: 2630 Fragments arranged by size: 2630 2198 718 580 496 472 327 127 88 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2132 2195 2437 3487 1163 Size: 573 396 63 242 1050 5312 Fragments arranged by size: 5312 1050 573 396 242 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4303 5086 5847 6875 1540 Size: 275 186 1372 930 783 761 1028 2301 Fragments arranged by size: 2301 1372 1028 930 783 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3184 3345 3386 3642 3783 3837 3924 4045 4752 Size: 161 41 256 141 54 87 121 707 Cuts at: 4752 5697 5782 6853 3184 Size: 945 85 1071 3967 Fragments arranged by size: 3967 1071 945 707 256 161 141 121 87 85 54 41 BsmI GAATG_Cn' Cuts at: 5147 5240 5147 Size: 93 7543 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3035 3184 3210 3345 3386 3446 3560 Size: 399 1245 149 26 135 41 60 114 Cuts at: 3560 3642 3783 3837 3924 4045 4062 4752 4826 Size: 82 141 54 87 121 17 690 74 Cuts at: 4826 4955 5697 5782 6853 1391 Size: 129 742 85 1071 2174 Fragments arranged by size: 2174 1245 1071 742 690 399 149 141 135 129 121 114 87 85 82 74 60 54 41 26 17 BspEI T'CCGG_A Cuts at: 3378 7299 3378 Size: 3921 3715 BspHI T'CATG_A Cuts at: 4371 4518 5525 7255 4371 Size: 147 1007 1730 4752 Fragments arranged by size: 4752 1730 1007 147 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2718 3063 3582 4083 4108 4991 844 Size: 1874 345 519 501 25 883 3489 Fragments arranged by size: 3489 1874 883 519 501 345 25 BsrBI CCG'CTC Cuts at: 1394 1823 3550 3792 4186 5523 1394 Size: 429 1727 242 394 1337 3507 Fragments arranged by size: 3507 1727 1337 429 394 242 BsrDI GCAATG_nn' Cuts at: 65 1248 2140 6131 6305 65 Size: 1183 892 3991 174 1396 Fragments arranged by size: 3991 1396 1183 892 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2424 3372 4767 4883 6280 6467 1700 Size: 110 614 948 1395 116 1397 187 2869 Fragments arranged by size: 2869 1397 1395 948 614 187 116 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7636 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7636 BssSI C'ACGA_G Cuts at: 3108 3260 5696 6708 3108 Size: 152 2436 1012 4036 Fragments arranged by size: 4036 2436 1012 152 BstAPI GCAn_nnn'nTGC Cuts at: 794 4704 794 Size: 3910 3726 BstBI TT'CG_AA Cuts at: 6440 6440 Size: 7636 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7636 BstXI CCAn_nnnn'nTGG Cuts at: 2995 3439 2995 Size: 444 7192 BstYI r'GATC_y Cuts at: 1972 3408 3669 3816 4386 4488 4966 5333 5718 Size: 1436 261 147 570 102 478 367 385 Cuts at: 5718 5735 7187 7273 7285 7631 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1436 570 478 385 367 346 261 147 102 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2286 4500 4659 4735 4826 513 Size: 552 939 282 2214 159 76 91 3323 Fragments arranged by size: 3323 2214 939 552 282 159 91 76 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3604 487 Size: 683 56 2378 4519 Fragments arranged by size: 4519 2378 683 56 BtsI GCAGTG_nn' Cuts at: 2562 2903 2948 3222 4168 4241 4531 4577 5152 Size: 341 45 274 946 73 290 46 575 Cuts at: 5152 5946 5966 2562 Size: 794 20 4232 Fragments arranged by size: 4232 946 794 575 341 290 274 73 46 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7636 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7601 DraI TTT'AAA Cuts at: 4940 4940 Size: 7636 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2780 6643 809 Size: 507 1464 3863 1802 Fragments arranged by size: 3863 1802 1464 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2997 3370 Size: 54 1425 50 275 18 369 798 373 Cuts at: 3370 4096 4300 4794 5083 5972 7601 8 Size: 726 204 494 289 889 1629 43 Fragments arranged by size: 1629 1425 889 798 726 494 373 369 289 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 4300 5083 1537 Size: 275 1558 930 783 4090 Fragments arranged by size: 4090 1558 930 783 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3584 5566 1353 Size: 2231 1982 3423 Fragments arranged by size: 3423 2231 1982 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 3809 6223 6597 6743 387 Size: 1064 138 453 1767 2414 374 146 1280 Fragments arranged by size: 2414 1767 1280 1064 453 374 146 138 EcoICRI GAG'CTC Cuts at: 3384 3922 4750 3384 Size: 538 828 6270 Fragments arranged by size: 6270 828 538 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 2926 3653 4126 4467 4673 4802 Size: 393 698 596 727 473 341 206 129 Cuts at: 4802 5699 6296 7083 7351 1239 Size: 897 597 787 268 1524 Fragments arranged by size: 1524 897 787 727 698 597 596 473 393 341 268 206 129 EcoNI CCTnn'n_nnAGG Cuts at: 3884 4449 3884 Size: 565 7071 EcoO109I rG'GnC_Cy Cuts at: 2523 2736 2898 3032 3302 3334 4138 4192 4455 Size: 213 162 134 270 32 804 54 263 Cuts at: 4455 4876 2523 Size: 421 5283 Fragments arranged by size: 5283 804 421 270 263 213 162 134 54 32 EcoRI G'AATT_C Cuts at: 4945 5323 4945 Size: 378 7258 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3262 3451 3772 3262 Size: 189 321 7126 Fragments arranged by size: 7126 321 189 FspI TGC'GCA Cuts at: 6142 6142 Size: 7636 HaeII r_GCGC'y Cuts at: 1678 2309 2330 6783 1678 Size: 631 21 4453 2531 Fragments arranged by size: 4453 2531 631 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2753 2785 2804 2836 3707 Size: 32 135 32 1181 32 19 32 871 Cuts at: 3707 3739 6277 6309 6351 6383 1373 Size: 32 2538 32 42 32 2626 Fragments arranged by size: 2626 2538 1181 871 135 42 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 2061 3105 4980 5226 669 Size: 988 281 123 1044 1875 246 3079 Fragments arranged by size: 3079 1875 1044 988 281 246 123 HindIII A'AGCT_T Cuts at: 748 748 Size: 7636 HpaI GTT'AAC Cuts at: 5226 5226 Size: 7636 KpnI G_GTAC'C Cuts at: 4961 4961 Size: 7636 MfeI C'AATT_G Cuts at: 5235 5235 Size: 7636 MluI A'CGCG_T Cuts at: 7512 7512 Size: 7636 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3759 4647 6750 6934 1305 Size: 2454 888 2103 184 2007 Fragments arranged by size: 2454 2103 2007 888 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4098 4796 10 Size: 54 1425 343 2266 698 2850 Fragments arranged by size: 2850 2266 1425 698 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4520 5594 5953 6112 518 Size: 648 3354 1074 359 159 2042 Fragments arranged by size: 3354 2042 1074 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2850 2875 3589 3894 4638 4728 5052 5729 Size: 1244 25 714 305 744 90 324 677 Cuts at: 5729 6877 7122 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1244 1148 744 714 677 324 305 245 90 25 NaeI GCC'GGC Cuts at: 3374 3374 Size: 7636 NcoI C'CATG_G Cuts at: 513 1065 2004 2286 4659 4735 513 Size: 552 939 282 2373 76 3414 Fragments arranged by size: 3414 2373 939 552 282 76 NdeI CA'TA_TG Cuts at: 387 387 Size: 7636 NgoMIV G'CCGG_C Cuts at: 3372 3372 Size: 7636 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7636 NotI GC'GGCC_GC Cuts at: 5083 5083 Size: 7636 NspI r_CATG'y Cuts at: 1132 1229 2261 3217 4016 4363 4399 4417 4816 Size: 97 1032 956 799 347 36 18 399 Cuts at: 4816 4994 6539 1132 Size: 178 1545 2229 Fragments arranged by size: 2229 1545 1032 956 799 399 347 178 97 36 18 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7636 PciI A'CATG_T Cuts at: 4359 4413 6535 4359 Size: 54 2122 5460 Fragments arranged by size: 5460 2122 54 PflMI CCAn_nnn'nTGG Cuts at: 1108 2491 2533 4318 4641 1108 Size: 1383 42 1785 323 4103 Fragments arranged by size: 4103 1785 1383 323 42 PfoI T'CCnGG_A Cuts at: 2145 2808 3828 4389 2145 Size: 663 1020 561 5392 Fragments arranged by size: 5392 1020 663 561 PmeI GTTT'AAAC Cuts at: 4940 4940 Size: 7636 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5705 5737 7218 7250 5705 Size: 32 1481 32 6091 Fragments arranged by size: 6091 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2736 2898 4138 4192 4455 4876 2736 Size: 162 1240 54 263 421 5496 Fragments arranged by size: 5496 1240 421 263 162 54 PshAI GACnn'nnGTC Cuts at: 1613 3165 1613 Size: 1552 6084 PsiI TTA'TAA Cuts at: 5206 5206 Size: 7636 PspOMI G'GGCC_C Cuts at: 3031 4058 3031 Size: 1027 6609 PspXI vC'TCGA_Gb Cuts at: 2646 2646 Size: 7636 PstI C_TGCA'G Cuts at: 830 2049 3942 4080 4988 830 Size: 1219 1893 138 908 3478 Fragments arranged by size: 3478 1893 1219 908 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7636 PvuII CAG'CTG Cuts at: 2875 3894 2875 Size: 1019 6617 SacI G_AGCT'C Cuts at: 3386 3924 4752 3386 Size: 538 828 6270 Fragments arranged by size: 6270 828 538 SalI G'TCGA_C Cuts at: 1936 2059 3103 4978 1936 Size: 123 1044 1875 4594 Fragments arranged by size: 4594 1875 1044 123 SanDI GG'GwC_CC Cuts at: 2898 4455 2898 Size: 1557 6079 SapI GCTCTTCn'nnn_ Cuts at: 3584 3584 Size: 7636 SbfI CC_TGCA'GG Cuts at: 4988 4988 Size: 7636 ScaI AGT'ACT Cuts at: 3809 3947 3992 4148 5884 7596 3809 Size: 138 45 156 1736 1712 3849 Fragments arranged by size: 3849 1736 1712 156 138 45 SexAI A'CCwGG_T Cuts at: 2220 2220 Size: 7636 SfcI C'TryA_G Cuts at: 826 1045 2045 3938 4076 4150 4984 6119 6800 Size: 219 1000 1893 138 74 834 1135 681 Cuts at: 6800 6991 826 Size: 191 1471 Fragments arranged by size: 1893 1471 1135 1000 834 681 219 191 138 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7636 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7636 SmaI CCC'GGG Cuts at: 1872 2237 3030 3308 4963 1872 Size: 365 793 278 1655 4545 Fragments arranged by size: 4545 1655 793 365 278 SmlI C'TyrA_G Cuts at: 820 1017 1943 2102 2646 3338 3803 3923 4142 Size: 197 926 159 544 692 465 120 219 Cuts at: 4142 5740 6641 6903 820 Size: 1598 901 262 1553 Fragments arranged by size: 1598 1553 926 901 692 544 465 262 219 197 159 120 SnaBI TAC'GTA Cuts at: 493 5427 493 Size: 4934 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7636 SphI G_CATG'C Cuts at: 1132 3217 4016 4994 1132 Size: 2085 799 978 3774 Fragments arranged by size: 3774 2085 978 799 SspI AAT'ATT Cuts at: 5 52 5560 5 Size: 47 5508 2081 Fragments arranged by size: 5508 2081 47 StuI AGG'CCT Cuts at: 1517 2093 2408 3275 1517 Size: 576 315 867 5878 Fragments arranged by size: 5878 867 576 315 StyI C'CwwG_G Cuts at: 513 1065 2004 2286 3246 3711 4158 4179 4209 Size: 552 939 282 960 465 447 21 30 Cuts at: 4209 4659 4735 4797 5075 513 Size: 450 76 62 278 3074 Fragments arranged by size: 3074 960 939 552 465 450 447 282 278 76 62 30 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2912 3272 3557 4028 4216 5663 5680 Size: 1255 1332 360 285 471 188 1447 17 Cuts at: 5680 5833 6018 6463 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1447 1332 1255 471 445 360 285 188 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 3807 3945 Size: 274 80 33 51 1443 201 1629 138 Cuts at: 3945 3990 4146 5882 7594 96 Size: 45 156 1736 1712 138 Fragments arranged by size: 1736 1712 1629 1443 274 201 156 138 138 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2231 2263 4574 4606 7596 7628 2231 Size: 32 2311 32 2990 32 2239 Fragments arranged by size: 2990 2311 2239 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7636 XbaI T'CTAG_A Cuts at: 4972 4972 Size: 7636 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4033 4033 Size: 7636 XhoI C'TCGA_G Cuts at: 1943 2646 1943 Size: 703 6933 XmaI C'CCGG_G Cuts at: 1870 2235 3028 3306 4961 1870 Size: 365 793 278 1655 4545 Fragments arranged by size: 4545 1655 793 365 278 XmnI GAAnn'nnTTC Cuts at: 4613 5765 4613 Size: 1152 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5444 276 Size: 53 83 186 936 3910 2468 Fragments arranged by size: 3910 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacII SfiI SfoI SrfI SwaI