Restriction map for
pFN21AA1016
(Linear) (Six-base) MAPSORT of: KIAA1016.seq Check: 5671 from: 1 to: 2184
KIAA1016.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 1791 2184
Size: 1791 393
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1660 2184
Size: 1660 524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 748 840 2184
Size: 748 92 1344
Fragments arranged by size:
1344 748 92
AlwNI CAG_nnn'CTG
Cuts at: 0 432 1981 2184
Size: 432 1549 203
Fragments arranged by size:
1549 432 203
ApaLI G'TGCA_C
Cuts at: 0 1291 2184
Size: 1291 893
ApoI r'AATT_y
Cuts at: 0 253 368 1123 1135 1228 1674 2184
Size: 253 115 755 12 93 446 510
Fragments arranged by size:
755 510 446 253 115 93 12
AvaI C'yCGr_G
Cuts at: 0 274 2059 2184
Size: 274 1785 125
Fragments arranged by size:
1785 274 125
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 375 408 2184
Size: 375 33 1776
Fragments arranged by size:
1776 375 33
BanI G'GyrC_C
Cuts at: 0 118 2184
Size: 118 2066
BbeI G_GCGC'C
Cuts at: 0 122 2184
Size: 122 2062
BbsI GAAGACnn'nnnn_
Cuts at: 0 1031 1262 1805 2184
Size: 1031 231 543 379
Fragments arranged by size:
1031 543 379 231
BglII A'GATC_T
Cuts at: 0 693 2184
Size: 693 1491
Bme1580I G_kGCm'C
Cuts at: 0 1295 2184
Size: 1295 889
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 797 1525 2082 2184
Size: 208 589 728 557 102
Fragments arranged by size:
728 589 557 208 102
Bpu10I CC'TnA_GC
Cuts at: 0 232 1105 2184
Size: 232 873 1079
Fragments arranged by size:
1079 873 232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 468 808 2184
Size: 220 248 340 1376
Fragments arranged by size:
1376 340 248 220
BsaI GGTCTCn'nnnn_
Cuts at: 0 1184 1222 2184
Size: 1184 38 962
Fragments arranged by size:
1184 962 38
BsaAI yAC'GTr
Cuts at: 0 939 2184
Size: 939 1245
BsaHI Gr'CG_yC
Cuts at: 0 7 119 2184
Size: 7 112 2065
Fragments arranged by size:
2065 112 7
BsaWI w'CCGG_w
Cuts at: 0 1143 1515 1706 2184
Size: 1143 372 191 478
Fragments arranged by size:
1143 478 372 191
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1078 1108 2184
Size: 1078 30 1076
Fragments arranged by size:
1078 1076 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 800 2184
Size: 217 583 1384
Fragments arranged by size:
1384 583 217
BseYI C'CCAG_C
Cuts at: 0 611 2184
Size: 611 1573
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 319 1466 2184
Size: 43 276 1147 718
Fragments arranged by size:
1147 718 276 43
BsiEI CG_ry'CG
Cuts at: 0 1867 2184
Size: 1867 317
BsiHKAI G_wGCw'C
Cuts at: 0 737 1295 2184
Size: 737 558 889
Fragments arranged by size:
889 737 558
BsmI GAATG_Cn'
Cuts at: 0 2175 2184
Size: 2175 9
Bsp1286I G_dGCh'C
Cuts at: 0 737 1295 2184
Size: 737 558 889
Fragments arranged by size:
889 737 558
BspEI T'CCGG_A
Cuts at: 0 1706 2184
Size: 1706 478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 176 2184
Size: 176 2008
BsrBI CCG'CTC
Cuts at: 0 2096 2184
Size: 2096 88
BsrDI GCAATG_nn'
Cuts at: 0 1355 2184
Size: 1355 829
BsrFI r'CCGG_y
Cuts at: 0 115 2184
Size: 115 2069
BssHII G'CGCG_C
Cuts at: 0 192 194 2184
Size: 192 2 1990
Fragments arranged by size:
1990 192 2
BssSI C'ACGA_G
Cuts at: 0 736 1092 2184
Size: 736 356 1092
Fragments arranged by size:
1092 736 356
BstBI TT'CG_AA
Cuts at: 0 2031 2184
Size: 2031 153
BstYI r'GATC_y
Cuts at: 0 693 1389 2184
Size: 693 696 795
Fragments arranged by size:
795 696 693
Bsu36I CC'TnA_GG
Cuts at: 0 407 2184
Size: 407 1777
BtgI C'CryG_G
Cuts at: 0 105 911 1861 2184
Size: 105 806 950 323
Fragments arranged by size:
950 806 323 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1084 2184
Size: 1084 1100
DraI TTT'AAA
Cuts at: 0 1433 2184
Size: 1433 751
EaeI y'GGCC_r
Cuts at: 0 209 1916 2184
Size: 209 1707 268
Fragments arranged by size:
1707 268 209
EarI CTCTTCn'nnn_
Cuts at: 0 539 1259 1277 1727 2184
Size: 539 720 18 450 457
Fragments arranged by size:
720 539 457 450 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 456 1846 2184
Size: 456 1390 338
Fragments arranged by size:
1390 456 338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 797 1525 1660 2082 2184
Size: 208 589 728 135 422 102
Fragments arranged by size:
728 589 422 208 135 102
EcoO109I rG'GnC_Cy
Cuts at: 0 2103 2184
Size: 2103 81
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 732 2184
Size: 732 1452
HaeII r_GCGC'y
Cuts at: 0 122 239 2184
Size: 122 117 1945
Fragments arranged by size:
1945 122 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 441 473 1769 1801 1975 2007 2184
Size: 441 32 1296 32 174 32 177
Fragments arranged by size:
1296 441 177 174 32 32 32
HincII GTy'rAC
Cuts at: 0 1848 2043 2184
Size: 1848 195 141
Fragments arranged by size:
1848 195 141
KasI G'GCGC_C
Cuts at: 0 118 2184
Size: 118 2066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 954 1994 2184
Size: 954 1040 190
Fragments arranged by size:
1040 954 190
MscI TGG'CCA
Cuts at: 0 1918 2184
Size: 1918 266
MslI CAynn'nnrTG
Cuts at: 0 1771 2168 2184
Size: 1771 397 16
Fragments arranged by size:
1771 397 16
MspA1I CmG'CkG
Cuts at: 0 155 462 587 771 889 1362 1503 1897
Size: 155 307 125 184 118 473 141 394
Cuts at: 1897 2184
Size: 287
Fragments arranged by size:
473 394 307 287 184 155 141 125
118
NarI GG'CG_CC
Cuts at: 0 119 2184
Size: 119 2065
NcoI C'CATG_G
Cuts at: 0 911 2184
Size: 911 1273
NspI r_CATG'y
Cuts at: 0 875 2184
Size: 875 1309
PflMI CCAn_nnn'nTGG
Cuts at: 0 912 939 2184
Size: 912 27 1245
Fragments arranged by size:
1245 912 27
PmlI CAC'GTG
Cuts at: 0 939 2184
Size: 939 1245
PpuMI rG'GwC_Cy
Cuts at: 0 2103 2184
Size: 2103 81
PstI C_TGCA'G
Cuts at: 0 428 806 1602 2012 2184
Size: 428 378 796 410 172
Fragments arranged by size:
796 428 410 378 172
PvuII CAG'CTG
Cuts at: 0 462 587 771 889 1362 1503 2184
Size: 462 125 184 118 473 141 681
Fragments arranged by size:
681 473 462 184 141 125 118
SapI GCTCTTCn'nnn_
Cuts at: 0 1259 2184
Size: 1259 925
SfcI C'TryA_G
Cuts at: 0 424 802 1598 1685 2008 2184
Size: 424 378 796 87 323 176
Fragments arranged by size:
796 424 378 323 176 87
SfoI GGC'GCC
Cuts at: 0 120 2184
Size: 120 2064
SmaI CCC'GGG
Cuts at: 0 276 2184
Size: 276 1908
SmlI C'TyrA_G
Cuts at: 0 199 447 787 2184
Size: 199 248 340 1397
Fragments arranged by size:
1397 340 248 199
SpeI A'CTAG_T
Cuts at: 0 687 2184
Size: 687 1497
SphI G_CATG'C
Cuts at: 0 875 2184
Size: 875 1309
StyI C'CwwG_G
Cuts at: 0 701 911 2184
Size: 701 210 1273
Fragments arranged by size:
1273 701 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 77 1150 2184
Size: 77 1073 1034
Fragments arranged by size:
1073 1034 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 2184
Size: 1078 32 1074
Fragments arranged by size:
1078 1074 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1911 2184
Size: 1911 273
XmaI C'CCGG_G
Cuts at: 0 274 2184
Size: 274 1910
XmnI GAAnn'nnTTC
Cuts at: 0 1754 2184
Size: 1754 430
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaLI ApoI AvaI BaeI
BanI BbeI BbsI BglII Bme1580I BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstYI Bsu36I BtgI BtgZI
DraI EaeI EarI EciI Eco57MI EcoO109I FalI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NarI
NcoI NspI PflMI PmlI PpuMI PstI PvuII SapI
SfcI SfoI SmaI SmlI SpeI SphI StyI TaqII
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BamHI BanII BbvCI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI BsaBI BsiWI BsmBI
BspHI BsrGI BstAPI BstEII BstXI BstZ17I BtsI ClaI
CspCI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI ZraI