Restriction map for
pFN21AA1003
(Linear) (Six-base) MAPSORT of: KIAA1003.seq Check: 9608 from: 1 to: 2739
KIAA1003.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2070 2739
Size: 2070 669
AclI AA'CG_TT
Cuts at: 0 1408 2739
Size: 1408 1331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2593 2739
Size: 2593 146
AfeI AGC'GCT
Cuts at: 0 1315 1759 2739
Size: 1315 444 980
Fragments arranged by size:
1315 980 444
AflIII A'CryG_T
Cuts at: 0 1664 2129 2330 2739
Size: 1664 465 201 409
Fragments arranged by size:
1664 465 409 201
AleI CACnn'nnGTG
Cuts at: 0 1399 2739
Size: 1399 1340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1738 2476 2739
Size: 1738 738 263
Fragments arranged by size:
1738 738 263
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 976 1008 2739
Size: 976 32 1731
Fragments arranged by size:
1731 976 32
AlwNI CAG_nnn'CTG
Cuts at: 0 285 486 582 1118 2682 2739
Size: 285 201 96 536 1564 57
Fragments arranged by size:
1564 536 285 201 96 57
ApaI G_GGCC'C
Cuts at: 0 2522 2689 2739
Size: 2522 167 50
Fragments arranged by size:
2522 167 50
ApaLI G'TGCA_C
Cuts at: 0 1854 2739
Size: 1854 885
AseI AT'TA_AT
Cuts at: 0 1952 2739
Size: 1952 787
AvaI C'yCGr_G
Cuts at: 0 422 448 779 861 1732 1816 2515 2647
Size: 422 26 331 82 871 84 699 132
Cuts at: 2647 2739
Size: 92
Fragments arranged by size:
871 699 422 331 132 92 84 82
26
BanI G'GyrC_C
Cuts at: 0 1248 1498 2739
Size: 1248 250 1241
Fragments arranged by size:
1248 1241 250
BanII G_rGCy'C
Cuts at: 0 307 740 832 1826 2111 2513 2522 2689
Size: 307 433 92 994 285 402 9 167
Cuts at: 2689 2739
Size: 50
Fragments arranged by size:
994 433 402 307 285 167 92 50
9
BbeI G_GCGC'C
Cuts at: 0 1502 2739
Size: 1502 1237
BbsI GAAGACnn'nnnn_
Cuts at: 0 1622 2739
Size: 1622 1117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1183 1217 2364 2398 2739
Size: 1183 34 1147 34 341
Fragments arranged by size:
1183 1147 341 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1401 1581 2739
Size: 1401 180 1158
Fragments arranged by size:
1401 1158 180
BglI GCCn_nnn'nGGC
Cuts at: 0 434 481 763 1904 2422 2517 2739
Size: 434 47 282 1141 518 95 222
Fragments arranged by size:
1141 518 434 282 222 95 47
Bme1580I G_kGCm'C
Cuts at: 0 1858 1925 2522 2689 2739
Size: 1858 67 597 167 50
Fragments arranged by size:
1858 597 167 67 50
BmgBI CAC'GTC
Cuts at: 0 1803 2739
Size: 1803 936
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1095 2739
Size: 295 800 1644
Fragments arranged by size:
1644 800 295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1249 2739
Size: 724 525 1490
Fragments arranged by size:
1490 724 525
BsaI GGTCTCn'nnnn_
Cuts at: 0 591 2602 2739
Size: 591 2011 137
Fragments arranged by size:
2011 591 137
BsaAI yAC'GTr
Cuts at: 0 621 1260 2031 2130 2331 2739
Size: 621 639 771 99 201 408
Fragments arranged by size:
771 639 621 408 201 99
BsaBI GATnn'nnATC
Cuts at: 0 2386 2656 2739
Size: 2386 270 83
Fragments arranged by size:
2386 270 83
BsaHI Gr'CG_yC
Cuts at: 0 1126 1499 2441 2739
Size: 1126 373 942 298
Fragments arranged by size:
1126 942 373 298
BsaWI w'CCGG_w
Cuts at: 0 106 824 2739
Size: 106 718 1915
Fragments arranged by size:
1915 718 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 2272 2302 2320 2350 2739
Size: 19 30 2223 30 18 30 389
Fragments arranged by size:
2223 389 30 30 30 19 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 297 748 1152 1873 2071 2443 2620 2739
Size: 297 451 404 721 198 372 177 119
Fragments arranged by size:
721 451 404 372 297 198 177 119
BseYI C'CCAG_C
Cuts at: 0 300 683 1088 1582 2110 2682 2739
Size: 300 383 405 494 528 572 57
Fragments arranged by size:
572 528 494 405 383 300 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1387 2032 2683 2739
Size: 712 675 645 651 56
Fragments arranged by size:
712 675 651 645 56
BsiHKAI G_wGCw'C
Cuts at: 0 740 1265 1858 2285 2338 2739
Size: 740 525 593 427 53 401
Fragments arranged by size:
740 593 525 427 401 53
BsiWI C'GTAC_G
Cuts at: 0 1256 2739
Size: 1256 1483
BsmI GAATG_Cn'
Cuts at: 0 1745 2739
Size: 1745 994
BsmBI CGTCTCn'nnnn_
Cuts at: 0 916 1809 1996 2719 2739
Size: 916 893 187 723 20
Fragments arranged by size:
916 893 723 187 20
Bsp1286I G_dGCh'C
Cuts at: 0 307 740 832 1265 1826 1858 1925 2111
Size: 307 433 92 433 561 32 67 186
Cuts at: 2111 2285 2338 2513 2522 2689 2739
Size: 174 53 175 9 167 50
Fragments arranged by size:
561 433 433 307 186 175 174 167
92 67 53 50 32 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 123 1838 2070 2705 2739
Size: 123 1715 232 635 34
Fragments arranged by size:
1715 635 232 123 34
BsrBI CCG'CTC
Cuts at: 0 975 1680 2739
Size: 975 705 1059
Fragments arranged by size:
1059 975 705
BsrFI r'CCGG_y
Cuts at: 0 747 1485 2739
Size: 747 738 1254
Fragments arranged by size:
1254 747 738
BsrGI T'GTAC_A
Cuts at: 0 1667 2288 2739
Size: 1667 621 451
Fragments arranged by size:
1667 621 451
BssSI C'ACGA_G
Cuts at: 0 730 1192 1226 1363 1768 1999 2739
Size: 730 462 34 137 405 231 740
Fragments arranged by size:
740 730 462 405 231 137 34
BstEII G'GTnAC_C
Cuts at: 0 48 102 820 2739
Size: 48 54 718 1919
Fragments arranged by size:
1919 718 54 48
BstXI CCAn_nnnn'nTGG
Cuts at: 0 119 1401 2739
Size: 119 1282 1338
Fragments arranged by size:
1338 1282 119
BstYI r'GATC_y
Cuts at: 0 1736 2739
Size: 1736 1003
BtgI C'CryG_G
Cuts at: 0 758 1058 1079 1102 1902 2208 2739
Size: 758 300 21 23 800 306 531
Fragments arranged by size:
800 758 531 306 300 23 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1914 2739
Size: 1914 825
BtsI GCAGTG_nn'
Cuts at: 0 873 1052 1375 1423 1923 1966 2470 2739
Size: 873 179 323 48 500 43 504 269
Fragments arranged by size:
873 504 500 323 269 179 48 43
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 602 637 2739
Size: 602 35 2102
Fragments arranged by size:
2102 602 35
DraIII CAC_nnn'GTG
Cuts at: 0 1773 2739
Size: 1773 966
DrdI GACnn_nn'nnGTC
Cuts at: 0 1984 2254 2739
Size: 1984 270 485
Fragments arranged by size:
1984 485 270
EaeI y'GGCC_r
Cuts at: 0 610 755 1451 1487 2093 2366 2559 2739
Size: 610 145 696 36 606 273 193 180
Fragments arranged by size:
696 610 606 273 193 180 145 36
EarI CTCTTCn'nnn_
Cuts at: 0 315 2041 2739
Size: 315 1726 698
Fragments arranged by size:
1726 698 315
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1317 2650 2739
Size: 1317 1333 89
Fragments arranged by size:
1333 1317 89
EcoICRI GAG'CTC
Cuts at: 0 738 2739
Size: 738 2001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1095 2593 2739
Size: 295 800 1498 146
Fragments arranged by size:
1498 800 295 146
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1846 2739
Size: 1846 893
EcoO109I rG'GnC_Cy
Cuts at: 0 15 483 1446 1597 2176 2739
Size: 15 468 963 151 579 563
Fragments arranged by size:
963 579 563 468 151 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 228 721 1639 2739
Size: 228 493 918 1100
Fragments arranged by size:
1100 918 493 228
FspI TGC'GCA
Cuts at: 0 541 2739
Size: 541 2198
FspAI rTGC'GCAy
Cuts at: 0 541 2739
Size: 541 2198
HaeII r_GCGC'y
Cuts at: 0 769 1317 1502 1761 2739
Size: 769 548 185 259 978
Fragments arranged by size:
978 769 548 259 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 382 414 762 794 835 867 1375 1407
Size: 382 32 348 32 41 32 508 32
Cuts at: 1407 1843 1870 1875 1902 2739
Size: 436 27 5 27 837
Fragments arranged by size:
837 508 436 382 348 41 32 32
32 32 27 27 5
HincII GTy'rAC
Cuts at: 0 660 2739
Size: 660 2079
KasI G'GCGC_C
Cuts at: 0 1498 2739
Size: 1498 1241
MfeI C'AATT_G
Cuts at: 0 492 2739
Size: 492 2247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 1581 2739
Size: 261 1320 1158
Fragments arranged by size:
1320 1158 261
MscI TGG'CCA
Cuts at: 0 757 1453 2368 2739
Size: 757 696 915 371
Fragments arranged by size:
915 757 696 371
MslI CAynn'nnrTG
Cuts at: 0 436 1057 1399 2458 2739
Size: 436 621 342 1059 281
Fragments arranged by size:
1059 621 436 342 281
MspA1I CmG'CkG
Cuts at: 0 282 504 726 1081 1586 1642 1673 2571
Size: 282 222 222 355 505 56 31 898
Cuts at: 2571 2682 2739
Size: 111 57
Fragments arranged by size:
898 505 355 282 222 222 111 57
56 31
NaeI GCC'GGC
Cuts at: 0 1487 2739
Size: 1487 1252
NarI GG'CG_CC
Cuts at: 0 1499 2739
Size: 1499 1240
NcoI C'CATG_G
Cuts at: 0 1058 2739
Size: 1058 1681
NgoMIV G'CCGG_C
Cuts at: 0 1485 2739
Size: 1485 1254
NspI r_CATG'y
Cuts at: 0 1668 2463 2739
Size: 1668 795 276
Fragments arranged by size:
1668 795 276
PasI CC'CwG_GG
Cuts at: 0 1524 2739
Size: 1524 1215
PciI A'CATG_T
Cuts at: 0 1664 2739
Size: 1664 1075
PflMI CCAn_nnn'nTGG
Cuts at: 0 582 2670 2739
Size: 582 2088 69
Fragments arranged by size:
2088 582 69
PfoI T'CCnGG_A
Cuts at: 0 272 1412 1434 2471 2739
Size: 272 1140 22 1037 268
Fragments arranged by size:
1140 1037 272 268 22
PpuMI rG'GwC_Cy
Cuts at: 0 15 1446 2739
Size: 15 1431 1293
Fragments arranged by size:
1431 1293 15
PspOMI G'GGCC_C
Cuts at: 0 2518 2685 2739
Size: 2518 167 54
Fragments arranged by size:
2518 167 54
PspXI vC'TCGA_Gb
Cuts at: 0 422 861 1816 2739
Size: 422 439 955 923
Fragments arranged by size:
955 923 439 422
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 445 477 2311 2343 2739
Size: 445 32 1834 32 396
Fragments arranged by size:
1834 445 396 32 32
PstI C_TGCA'G
Cuts at: 0 134 482 1177 2739
Size: 134 348 695 1562
Fragments arranged by size:
1562 695 348 134
PvuII CAG'CTG
Cuts at: 0 726 1586 1673 2571 2739
Size: 726 860 87 898 168
Fragments arranged by size:
898 860 726 168 87
SacI G_AGCT'C
Cuts at: 0 740 2739
Size: 740 1999
SacII CC_GC'GG
Cuts at: 0 1082 2739
Size: 1082 1657
SapI GCTCTTCn'nnn_
Cuts at: 0 2041 2739
Size: 2041 698
SbfI CC_TGCA'GG
Cuts at: 0 482 2739
Size: 482 2257
ScaI AGT'ACT
Cuts at: 0 1711 2739
Size: 1711 1028
SexAI A'CCwGG_T
Cuts at: 0 2249 2739
Size: 2249 490
SfcI C'TryA_G
Cuts at: 0 130 478 1173 2040 2739
Size: 130 348 695 867 699
Fragments arranged by size:
867 699 695 348 130
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2422 2739
Size: 2422 317
SfoI GGC'GCC
Cuts at: 0 1500 2739
Size: 1500 1239
SmaI CCC'GGG
Cuts at: 0 2517 2739
Size: 2517 222
SmlI C'TyrA_G
Cuts at: 0 422 739 861 1264 1816 2739
Size: 422 317 122 403 552 923
Fragments arranged by size:
923 552 422 403 317 122
SnaBI TAC'GTA
Cuts at: 0 2031 2739
Size: 2031 708
SphI G_CATG'C
Cuts at: 0 2463 2739
Size: 2463 276
StuI AGG'CCT
Cuts at: 0 428 2416 2739
Size: 428 1988 323
Fragments arranged by size:
1988 428 323
StyI C'CwwG_G
Cuts at: 0 1058 1847 2739
Size: 1058 789 892
Fragments arranged by size:
1058 892 789
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 709 1878 2739
Size: 709 1169 861
Fragments arranged by size:
1169 861 709
TatI w'GTAC_w
Cuts at: 0 1550 1667 1709 1778 2288 2739
Size: 1550 117 42 69 510 451
Fragments arranged by size:
1550 510 451 117 69 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 19 51 2739
Size: 19 32 2688
Fragments arranged by size:
2688 32 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2346 2739
Size: 2346 393
XhoI C'TCGA_G
Cuts at: 0 422 861 1816 2739
Size: 422 439 955 923
Fragments arranged by size:
955 923 439 422
XmaI C'CCGG_G
Cuts at: 0 2515 2739
Size: 2515 224
Enzymes that do cut:
AarI AclI AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI AseI AvaI BanI BanII BbeI
BbsI BcgI BciVI BglI Bme1580I BmgBI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BsrGI BssSI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I
HincII KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PpuMI PspOMI PspXI PsrI PstI PvuII SacI SacII
SapI SbfI ScaI SexAI SfcI SfiI SfoI SmaI
SmlI SnaBI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AflII AgeI AhdI ApoI AscI
AsiSI AvrII BaeI BamHI BbvCI BclI BfrBI BglII
BlpI BmrI BmtI BplI Bpu10I BsiEI BspEI BspHI
BsrDI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI DraI
EagI EcoRI EcoRV FseI HindIII HpaI KpnI MluI
NdeI NheI NotI NruI NsiI PacI PmeI PmlI
PpiI PshAI PsiI PvuI RsrII SalI SanDI SgfI
SgrAI SpeI SrfI SspI SwaI Tth111I XbaI XmnI
ZraI