(Circular) (Six-base) MAPSORT of: pFN21AA0996.seq Check: 1667 from: 1 to: 7249 pFN21AA0996 7249 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4402 4402 Size: 7249 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2097 5059 278 Size: 53 83 186 936 561 2962 2468 Fragments arranged by size: 2962 2468 936 561 186 83 53 AccI GT'mk_AC Cuts at: 1937 2058 4592 1937 Size: 121 2534 4594 Fragments arranged by size: 4594 2534 121 Acc65I G'GTAC_C Cuts at: 4570 4570 Size: 7249 AclI AA'CG_TT Cuts at: 1563 5376 5749 1563 Size: 3813 373 3063 Fragments arranged by size: 3813 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2378 2691 3771 4524 5312 6696 6964 1632 Size: 746 313 1080 753 788 1384 268 1917 Fragments arranged by size: 1917 1384 1080 788 753 746 313 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7052 AflIII A'CryG_T Cuts at: 1203 6148 7125 1203 Size: 4945 977 1327 Fragments arranged by size: 4945 1327 977 AgeI A'CCGG_T Cuts at: 6080 6080 Size: 7249 AhdI GACnn_n'nnGTC Cuts at: 5978 5978 Size: 7249 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7249 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2504 1184 Size: 1320 5929 AlwNI CAG_nnn'CTG Cuts at: 2569 4992 6564 2569 Size: 2423 1572 3254 Fragments arranged by size: 3254 2423 1572 ApaI G_GGCC'C Cuts at: 2091 2166 2091 Size: 75 7174 ApaLI G'TGCA_C Cuts at: 5306 6462 5306 Size: 1156 6093 ApoI r'AATT_y Cuts at: 1493 2677 2936 2959 3726 3734 4558 4784 4936 Size: 1184 259 23 767 8 824 226 152 Cuts at: 4936 7045 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1184 824 767 259 226 152 23 8 AseI AT'TA_AT Cuts at: 160 1032 3621 5803 160 Size: 872 2589 2182 1606 Fragments arranged by size: 2589 2182 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7249 AvaI C'yCGr_G Cuts at: 1870 1943 3132 4574 1870 Size: 73 1189 1442 4545 Fragments arranged by size: 4545 1442 1189 73 BamHI G'GATC_C Cuts at: 4579 4946 4579 Size: 367 6882 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4570 6025 618 Size: 325 217 627 2783 1455 1842 Fragments arranged by size: 2783 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2091 2166 4568 1391 Size: 700 75 2402 4072 Fragments arranged by size: 4072 2402 700 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2316 2850 3063 3954 4323 928 Size: 971 417 534 213 891 369 3854 Fragments arranged by size: 3854 971 891 534 417 369 213 BbvCI CC'TCA_GC Cuts at: 2013 2013 Size: 7249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3420 3454 5440 5474 1903 Size: 34 50 34 1399 34 1986 34 3678 Fragments arranged by size: 3678 1986 1399 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3808 5141 6351 3808 Size: 1333 1210 4706 Fragments arranged by size: 4706 1333 1210 BclI T'GATC_A Cuts at: 1548 3649 1548 Size: 2101 5148 BfrBI ATG'CAT Cuts at: 3523 3523 Size: 7249 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2132 5860 136 Size: 107 122 71 631 605 460 3728 1525 Fragments arranged by size: 3728 1525 631 605 460 122 107 71 BglII A'GATC_T Cuts at: 7244 7244 Size: 7249 BlpI GC'TnA_GC Cuts at: 2219 4666 2219 Size: 2447 4802 Bme1580I G_kGCm'C Cuts at: 1790 2091 2166 2324 5310 6466 1790 Size: 301 75 158 2986 1156 2573 Fragments arranged by size: 2986 2573 1156 301 158 75 BmgBI CAC'GTC Cuts at: 1318 2233 2260 1318 Size: 915 27 6307 Fragments arranged by size: 6307 915 27 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4330 4980 5938 Size: 416 53 83 399 24 2913 650 958 Cuts at: 5938 442 Size: 1753 Fragments arranged by size: 2913 1753 958 650 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7249 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2459 3263 3849 5909 1239 Size: 977 243 804 586 2060 2579 Fragments arranged by size: 2579 2060 977 804 586 243 Bpu10I CC'TnA_GC Cuts at: 2013 2456 2013 Size: 443 6806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2507 5374 6239 6537 2507 Size: 2867 865 298 3219 Fragments arranged by size: 3219 2867 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5912 882 Size: 617 4413 2219 Fragments arranged by size: 4413 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3635 5040 493 Size: 711 2431 1405 2702 Fragments arranged by size: 2702 2431 1405 711 BsaBI GATnn'nnATC Cuts at: 4265 7243 4265 Size: 2978 4271 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 5056 5438 6073 Size: 53 83 186 936 561 2962 382 635 Cuts at: 6073 275 Size: 1451 Fragments arranged by size: 2962 1451 936 635 561 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2721 5682 6080 6354 6501 6912 2721 Size: 2961 398 274 147 411 3058 Fragments arranged by size: 3058 2961 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3964 3994 4158 4188 1178 Size: 30 165 30 2561 30 164 30 4239 Fragments arranged by size: 4239 2561 165 164 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2456 3541 3782 4010 4013 1186 Size: 1270 1085 241 228 3 4422 Fragments arranged by size: 4422 1270 1085 241 228 3 BseYI C'CCAG_C Cuts at: 2147 2562 3376 6452 6948 2147 Size: 415 814 3076 496 2448 Fragments arranged by size: 3076 2448 814 496 415 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2384 2414 2766 2817 3368 3817 1163 Size: 573 648 30 352 51 551 449 4595 Fragments arranged by size: 4595 648 573 551 449 352 51 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4699 5460 6488 1540 Size: 275 186 2698 761 1028 2301 Fragments arranged by size: 2698 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5310 5395 6466 5310 Size: 85 1071 6093 Fragments arranged by size: 6093 1071 85 BsmI GAATG_Cn' Cuts at: 3376 3431 4760 4853 3376 Size: 55 1329 93 5772 Fragments arranged by size: 5772 1329 93 55 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2166 2324 4568 5310 5395 6466 Size: 399 301 75 158 2244 742 85 1071 Cuts at: 6466 1391 Size: 2174 Fragments arranged by size: 2244 2174 1071 742 399 301 158 85 75 BspEI T'CCGG_A Cuts at: 2721 6912 2721 Size: 4191 3058 BspHI T'CATG_A Cuts at: 2283 5138 6868 2283 Size: 2855 1730 2664 Fragments arranged by size: 2855 2664 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4402 4604 844 Size: 3558 202 3489 Fragments arranged by size: 3558 3489 202 BsrBI CCG'CTC Cuts at: 1394 1823 2068 2446 5136 1394 Size: 429 245 378 2690 3507 Fragments arranged by size: 3507 2690 429 378 245 BsrDI GCAATG_nn' Cuts at: 65 1248 3241 5744 5918 65 Size: 1183 1993 2503 174 1396 Fragments arranged by size: 2503 1993 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5893 6080 1700 Size: 110 4083 187 2869 Fragments arranged by size: 4083 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7249 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7249 BssSI C'ACGA_G Cuts at: 3044 5309 6321 3044 Size: 2265 1012 3972 Fragments arranged by size: 3972 2265 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2789 794 Size: 1995 5254 BstBI TT'CG_AA Cuts at: 2954 3202 6053 2954 Size: 248 2851 4150 Fragments arranged by size: 4150 2851 248 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7249 BstXI CCAn_nnnn'nTGG Cuts at: 2233 2233 Size: 7249 BstYI r'GATC_y Cuts at: 1972 3943 4579 4946 5331 5348 6800 6886 6898 Size: 1971 636 367 385 17 1452 86 12 Cuts at: 6898 7244 1972 Size: 346 1977 Fragments arranged by size: 1977 1971 1452 636 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4452 4452 Size: 7249 BtgI C'CryG_G Cuts at: 513 1065 2115 2130 2189 513 Size: 552 1050 15 59 5573 Fragments arranged by size: 5573 1050 552 59 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4199 487 Size: 683 56 2973 3537 Fragments arranged by size: 3537 2973 683 56 BtsI GCAGTG_nn' Cuts at: 2326 2461 3805 4765 5559 5579 2326 Size: 135 1344 960 794 20 3996 Fragments arranged by size: 3996 1344 960 794 135 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7249 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7214 DraI TTT'AAA Cuts at: 2941 3052 4553 2941 Size: 111 1501 5637 Fragments arranged by size: 5637 1501 111 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6256 809 Size: 507 4940 1802 Fragments arranged by size: 4940 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 4696 5585 Size: 54 1425 50 275 18 273 2593 889 Cuts at: 5585 7214 8 Size: 1629 43 Fragments arranged by size: 2593 1629 1425 889 275 273 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4696 1537 Size: 275 2884 4090 Fragments arranged by size: 4090 2884 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2523 2742 3255 3536 3790 4338 5179 1353 Size: 1170 219 513 281 254 548 841 3423 Fragments arranged by size: 3423 1170 841 548 513 281 254 219 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5836 6210 6356 387 Size: 1064 138 4247 374 146 1280 Fragments arranged by size: 4247 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2378 2459 2691 3263 3771 3849 Size: 393 584 162 81 232 572 508 78 Cuts at: 3849 4524 5312 5909 6696 6964 1239 Size: 675 788 597 787 268 1524 Fragments arranged by size: 1524 788 787 675 597 584 572 508 393 268 232 162 81 78 EcoNI CCTnn'n_nnAGG Cuts at: 3560 3560 Size: 7249 EcoO109I rG'GnC_Cy Cuts at: 2087 2162 2163 2087 Size: 75 1 7173 Fragments arranged by size: 7173 75 1 EcoRI G'AATT_C Cuts at: 2959 3734 4558 4936 2959 Size: 775 824 378 5272 Fragments arranged by size: 5272 824 775 378 EcoRV GAT'ATC Cuts at: 4350 4350 Size: 7249 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3552 4096 3552 Size: 544 6705 FspI TGC'GCA Cuts at: 5755 5755 Size: 7249 HaeII r_GCGC'y Cuts at: 1678 2226 6396 1678 Size: 548 4170 2531 Fragments arranged by size: 4170 2531 548 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3205 3237 3289 3321 3893 Size: 32 135 32 1633 32 52 32 572 Cuts at: 3893 3925 4316 4348 5890 5922 5964 5996 1373 Size: 32 391 32 1542 32 42 32 2626 Fragments arranged by size: 2626 1633 1542 572 391 135 52 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4593 4839 669 Size: 988 281 2655 246 3079 Fragments arranged by size: 3079 2655 988 281 246 HindIII A'AGCT_T Cuts at: 748 2634 3534 748 Size: 1886 900 4463 Fragments arranged by size: 4463 1886 900 HpaI GTT'AAC Cuts at: 4839 4839 Size: 7249 KpnI G_GTAC'C Cuts at: 4574 4574 Size: 7249 MfeI C'AATT_G Cuts at: 4848 4848 Size: 7249 MluI A'CGCG_T Cuts at: 7125 7125 Size: 7249 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3042 6363 6547 1305 Size: 1737 3321 184 2007 Fragments arranged by size: 3321 2007 1737 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5427 Fragments arranged by size: 5427 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 2610 2793 5207 5566 5725 518 Size: 648 1065 379 183 2414 359 159 2042 Fragments arranged by size: 2414 2042 1065 648 379 359 183 159 MspA1I CmG'CkG Cuts at: 1606 2021 2076 2117 2191 2428 2569 3756 4665 Size: 415 55 41 74 237 141 1187 909 Cuts at: 4665 5342 6490 6735 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1187 1148 909 677 415 245 237 141 74 55 41 NcoI C'CATG_G Cuts at: 513 1065 2130 513 Size: 552 1065 5632 Fragments arranged by size: 5632 1065 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7249 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7249 NotI GC'GGCC_GC Cuts at: 4696 4696 Size: 7249 NsiI A_TGCA'T Cuts at: 3525 3525 Size: 7249 NspI r_CATG'y Cuts at: 1132 1229 2041 2057 4607 6152 1132 Size: 97 812 16 2550 1545 2229 Fragments arranged by size: 2550 2229 1545 812 97 16 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7249 PciI A'CATG_T Cuts at: 6148 6148 Size: 7249 PflMI CCAn_nnn'nTGG Cuts at: 1108 4241 1108 Size: 3133 4116 PfoI T'CCnGG_A Cuts at: 3279 3279 Size: 7249 PmeI GTTT'AAAC Cuts at: 4553 4553 Size: 7249 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5318 5350 6831 6863 5318 Size: 32 1481 32 5704 Fragments arranged by size: 5704 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7249 PsiI TTA'TAA Cuts at: 3302 4819 3302 Size: 1517 5732 PspOMI G'GGCC_C Cuts at: 2087 2162 2087 Size: 75 7174 PspXI vC'TCGA_Gb Cuts at: 3132 3132 Size: 7249 PstI C_TGCA'G Cuts at: 830 2274 2757 4601 830 Size: 1444 483 1844 3478 Fragments arranged by size: 3478 1844 1444 483 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7249 PvuII CAG'CTG Cuts at: 2428 2569 3756 2428 Size: 141 1187 5921 Fragments arranged by size: 5921 1187 141 SacII CC_GC'GG Cuts at: 2118 2192 2118 Size: 74 7175 SalI G'TCGA_C Cuts at: 1936 4591 1936 Size: 2655 4594 SapI GCTCTTCn'nnn_ Cuts at: 2742 3255 2742 Size: 513 6736 SbfI CC_TGCA'GG Cuts at: 4601 4601 Size: 7249 ScaI AGT'ACT Cuts at: 2393 3666 5497 7209 2393 Size: 1273 1831 1712 2433 Fragments arranged by size: 2433 1831 1712 1273 SexAI A'CCwGG_T Cuts at: 2762 2762 Size: 7249 SfcI C'TryA_G Cuts at: 826 1045 2270 2753 4597 5732 6413 6604 826 Size: 219 1225 483 1844 1135 681 191 1471 Fragments arranged by size: 1844 1471 1225 1135 681 483 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2132 2132 Size: 7249 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7249 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7249 SmaI CCC'GGG Cuts at: 1872 4576 1872 Size: 2704 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2522 3132 5353 6254 6516 820 Size: 197 926 579 610 2221 901 262 1553 Fragments arranged by size: 2221 1553 926 901 610 579 262 197 SnaBI TAC'GTA Cuts at: 493 5040 493 Size: 4547 2702 SpeI A'CTAG_T Cuts at: 152 2893 152 Size: 2741 4508 SphI G_CATG'C Cuts at: 1132 2041 4607 1132 Size: 909 2566 3774 Fragments arranged by size: 3774 2566 909 SspI AAT'ATT Cuts at: 5 52 5173 5 Size: 47 5121 2081 Fragments arranged by size: 5121 2081 47 StuI AGG'CCT Cuts at: 1517 2621 1517 Size: 1104 6145 StyI C'CwwG_G Cuts at: 513 1065 2130 2811 3612 4688 513 Size: 552 1065 681 801 1076 3074 Fragments arranged by size: 3074 1076 1065 801 681 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2576 4133 5276 5293 5446 5631 6076 Size: 1255 996 1557 1143 17 153 185 445 Cuts at: 6076 325 Size: 1498 Fragments arranged by size: 1557 1498 1255 1143 996 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2391 3664 5495 Size: 274 80 33 51 1443 414 1273 1831 Cuts at: 5495 7207 96 Size: 1712 138 Fragments arranged by size: 1831 1712 1443 1273 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2397 2429 7209 7241 2397 Size: 32 4780 32 2405 Fragments arranged by size: 4780 2405 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7249 XbaI T'CTAG_A Cuts at: 4585 4585 Size: 7249 XhoI C'TCGA_G Cuts at: 1943 3132 1943 Size: 1189 6060 XmaI C'CCGG_G Cuts at: 1870 4574 1870 Size: 2704 4545 XmnI GAAnn'nnTTC Cuts at: 3961 4473 5378 3961 Size: 512 905 5832 Fragments arranged by size: 5832 905 512 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2095 5057 276 Size: 53 83 186 936 561 2962 2468 Fragments arranged by size: 2962 2468 936 561 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PsrI RsrII SacI SanDI SfoI SrfI SwaI XcmI