(Circular) (Six-base) MAPSORT of: pFN21AA0985.seq Check: 5086 from: 1 to: 6787 pFN21AA0985 6787 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3616 3616 Size: 6787 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4597 278 Size: 53 83 186 936 3061 2468 Fragments arranged by size: 3061 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4130 1937 Size: 2193 4594 Acc65I G'GTAC_C Cuts at: 4108 4108 Size: 6787 AclI AA'CG_TT Cuts at: 1563 4914 5287 1563 Size: 3351 373 3063 Fragments arranged by size: 3351 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3281 3536 4850 6234 6502 1632 Size: 1649 255 1314 1384 268 1917 Fragments arranged by size: 1917 1649 1384 1314 268 255 AfeI AGC'GCT Cuts at: 3995 4046 3995 Size: 51 6736 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6590 AflIII A'CryG_T Cuts at: 1203 4074 5686 6663 1203 Size: 2871 1612 977 1327 Fragments arranged by size: 2871 1612 1327 977 AgeI A'CCGG_T Cuts at: 5618 5618 Size: 6787 AhdI GACnn_n'nnGTC Cuts at: 5516 5516 Size: 6787 AleI CACnn'nnGTG Cuts at: 1166 2115 1166 Size: 949 5838 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3753 1184 Size: 2569 4218 AlwNI CAG_nnn'CTG Cuts at: 2657 2669 4530 6102 2657 Size: 12 1861 1572 3342 Fragments arranged by size: 3342 1861 1572 12 ApaI G_GGCC'C Cuts at: 2639 2639 Size: 6787 ApaLI G'TGCA_C Cuts at: 3235 3728 4844 6000 3235 Size: 493 1116 1156 4022 Fragments arranged by size: 4022 1156 1116 493 ApoI r'AATT_y Cuts at: 1493 3194 3464 3479 3535 3851 4096 4322 4474 Size: 1701 270 15 56 316 245 226 152 Cuts at: 4474 6583 1493 Size: 2109 1697 Fragments arranged by size: 2109 1701 1697 316 270 245 226 152 56 15 AseI AT'TA_AT Cuts at: 160 1032 5341 160 Size: 872 4309 1606 Fragments arranged by size: 4309 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6787 AvaI C'yCGr_G Cuts at: 1870 1943 2193 2598 2610 2638 2675 2988 3597 Size: 73 250 405 12 28 37 313 609 Cuts at: 3597 4112 1870 Size: 515 4545 Fragments arranged by size: 4545 609 515 405 313 250 73 37 28 12 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4000 4033 4000 Size: 33 6754 BamHI G'GATC_C Cuts at: 4117 4484 4117 Size: 367 6420 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3185 4050 4108 5563 618 Size: 325 217 627 1398 865 58 1455 1842 Fragments arranged by size: 1842 1455 1398 865 627 325 217 58 BanII G_rGCy'C Cuts at: 1391 2333 2586 2605 2639 2732 2795 2861 4106 Size: 942 253 19 34 93 63 66 1245 Cuts at: 4106 1391 Size: 4072 Fragments arranged by size: 4072 1245 942 253 93 66 63 34 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2531 2654 3437 928 Size: 971 632 123 783 4278 Fragments arranged by size: 4278 971 783 632 123 BbvCI CC'TCA_GC Cuts at: 2871 2871 Size: 6787 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4978 5012 1903 Size: 34 3041 34 3678 Fragments arranged by size: 3678 3041 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4679 5889 4679 Size: 1210 5577 BclI T'GATC_A Cuts at: 1548 1548 Size: 6787 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2131 2717 5398 Size: 107 122 71 631 605 459 586 2681 Cuts at: 5398 136 Size: 1525 Fragments arranged by size: 2681 1525 631 605 586 459 122 107 71 BglII A'GATC_T Cuts at: 6782 6782 Size: 6787 BlpI GC'TnA_GC Cuts at: 4204 4204 Size: 6787 Bme1580I G_kGCm'C Cuts at: 1790 2639 3190 3239 3732 4848 6004 1790 Size: 849 551 49 493 1116 1156 2573 Fragments arranged by size: 2573 1156 1116 849 551 493 49 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6787 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2046 4518 5476 Size: 416 53 83 399 24 629 2472 958 Cuts at: 5476 442 Size: 1753 Fragments arranged by size: 2472 1753 958 629 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6787 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2298 2484 2589 2767 2796 2815 3572 Size: 836 223 186 105 178 29 19 757 Cuts at: 3572 5447 1239 Size: 1875 2579 Fragments arranged by size: 2579 1875 836 757 223 186 178 105 29 19 Bpu10I CC'TnA_GC Cuts at: 2580 2871 2580 Size: 291 6496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3491 4912 5777 6075 3491 Size: 1421 865 298 4203 Fragments arranged by size: 4203 1421 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2384 3380 3485 3676 5450 882 Size: 617 885 996 105 191 1774 2219 Fragments arranged by size: 2219 1774 996 885 617 191 105 BsaAI yAC'GTr Cuts at: 493 1204 4075 4578 493 Size: 711 2871 503 2702 Fragments arranged by size: 2871 2702 711 503 BsaBI GATnn'nnATC Cuts at: 6781 6781 Size: 6787 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2236 4594 4976 5611 Size: 53 83 186 936 703 2358 382 635 Cuts at: 5611 275 Size: 1451 Fragments arranged by size: 2358 1451 936 703 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3062 3790 5220 5618 5892 6039 6450 3062 Size: 728 1430 398 274 147 411 3399 Fragments arranged by size: 3399 1430 728 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2271 2301 2484 2514 3209 Size: 30 165 30 868 30 183 30 695 Cuts at: 3209 3239 1178 Size: 30 4726 Fragments arranged by size: 4726 868 695 183 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 3008 3146 3149 3665 3875 1186 Size: 991 831 138 3 516 210 4098 Fragments arranged by size: 4098 991 831 516 210 138 3 BseYI C'CCAG_C Cuts at: 2317 2326 2535 2662 2754 2794 2860 3022 3096 Size: 9 209 127 92 40 66 162 74 Cuts at: 3096 3578 3970 5990 6486 2317 Size: 482 392 2020 496 2618 Fragments arranged by size: 2618 2020 496 482 392 209 162 127 92 74 66 40 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2356 2873 3290 1163 Size: 573 620 517 417 4660 Fragments arranged by size: 4660 620 573 517 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4237 4998 6026 1540 Size: 275 186 2236 761 1028 2301 Fragments arranged by size: 2301 2236 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2732 3239 3732 4848 4933 6004 2732 Size: 507 493 1116 85 1071 3515 Fragments arranged by size: 3515 1116 1071 507 493 85 BsmI GAATG_Cn' Cuts at: 2407 4298 4391 2407 Size: 1891 93 4803 Fragments arranged by size: 4803 1891 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2800 2800 Size: 6787 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2333 2586 2605 2639 2732 2795 2861 Size: 399 543 253 19 34 93 63 66 Cuts at: 2861 3190 3239 3732 4106 4848 4933 6004 1391 Size: 329 49 493 374 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 543 493 399 374 329 253 93 85 66 63 49 34 19 BspEI T'CCGG_A Cuts at: 3062 6450 3062 Size: 3388 3399 BspHI T'CATG_A Cuts at: 4676 6406 4676 Size: 1730 5057 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2789 2845 3616 4142 844 Size: 1945 56 771 526 3489 Fragments arranged by size: 3489 1945 771 526 56 BsrBI CCG'CTC Cuts at: 1394 1823 4674 1394 Size: 429 2851 3507 Fragments arranged by size: 3507 2851 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5282 5456 65 Size: 1183 4034 174 1396 Fragments arranged by size: 4034 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2058 5431 5618 1700 Size: 110 248 3373 187 2869 Fragments arranged by size: 3373 2869 248 187 110 BsrGI T'GTAC_A Cuts at: 96 3681 96 Size: 3585 3202 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6787 BssSI C'ACGA_G Cuts at: 3392 4847 5859 3392 Size: 1455 1012 4320 Fragments arranged by size: 4320 1455 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2379 2430 3313 794 Size: 1585 51 883 4268 Fragments arranged by size: 4268 1585 883 51 BstBI TT'CG_AA Cuts at: 5591 5591 Size: 6787 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6787 BstXI CCAn_nnnn'nTGG Cuts at: 2117 2117 Size: 6787 BstYI r'GATC_y Cuts at: 1972 3441 3663 3975 4117 4484 4869 4886 6338 Size: 1469 222 312 142 367 385 17 1452 Cuts at: 6338 6424 6436 6782 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1469 1452 385 367 346 312 222 142 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3437 3437 Size: 6787 BtgI C'CryG_G Cuts at: 513 1065 3267 3741 513 Size: 552 2202 474 3559 Fragments arranged by size: 3559 2202 552 474 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6048 Fragments arranged by size: 6048 683 56 BtsI GCAGTG_nn' Cuts at: 2976 3239 4303 5097 5117 2976 Size: 263 1064 794 20 4646 Fragments arranged by size: 4646 1064 794 263 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6787 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6752 DraI TTT'AAA Cuts at: 4091 4091 Size: 6787 DraIII CAC_nnn'GTG Cuts at: 2379 2379 Size: 6787 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3589 3770 3917 5794 809 Size: 507 2273 181 147 1877 1802 Fragments arranged by size: 2273 1877 1802 507 181 147 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3469 4234 5123 Size: 54 1425 50 275 18 1639 765 889 Cuts at: 5123 6752 8 Size: 1629 43 Fragments arranged by size: 1639 1629 1425 889 765 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4234 1537 Size: 275 2422 4090 Fragments arranged by size: 4090 2422 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2142 2205 3135 3615 4717 1353 Size: 789 63 930 480 1102 3423 Fragments arranged by size: 3423 1102 930 789 480 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5374 5748 5894 387 Size: 1064 138 3785 374 146 1280 Fragments arranged by size: 3785 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2730 2730 Size: 6787 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2298 2484 2589 2767 2796 2815 Size: 393 443 223 186 105 178 29 19 Cuts at: 2815 3281 3536 3572 4850 5447 6234 6502 1239 Size: 466 255 36 1278 597 787 268 1524 Fragments arranged by size: 1524 1278 787 597 466 443 393 268 255 223 186 178 105 36 29 19 EcoNI CCTnn'n_nnAGG Cuts at: 2841 3614 2841 Size: 773 6014 EcoO109I rG'GnC_Cy Cuts at: 2497 2635 2654 2709 2889 2912 3256 2497 Size: 138 19 55 180 23 344 6028 Fragments arranged by size: 6028 344 180 138 55 23 19 EcoRI G'AATT_C Cuts at: 3194 3851 4096 4474 3194 Size: 657 245 378 5507 Fragments arranged by size: 5507 657 378 245 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3856 3856 Size: 6787 FspI TGC'GCA Cuts at: 2705 5293 2705 Size: 2588 4199 HaeII r_GCGC'y Cuts at: 1678 2867 3997 4048 5934 1678 Size: 1189 1130 51 1886 2531 Fragments arranged by size: 2531 1886 1189 1130 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2651 2683 3209 3241 3489 Size: 32 135 32 1079 32 526 32 248 Cuts at: 3489 3521 5428 5460 5502 5534 1373 Size: 32 1907 32 42 32 2626 Fragments arranged by size: 2626 1907 1079 526 248 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4131 4377 669 Size: 988 281 2193 246 3079 Fragments arranged by size: 3079 2193 988 281 246 HindIII A'AGCT_T Cuts at: 748 3338 748 Size: 2590 4197 HpaI GTT'AAC Cuts at: 4377 4377 Size: 6787 KpnI G_GTAC'C Cuts at: 4112 4112 Size: 6787 MfeI C'AATT_G Cuts at: 4386 4386 Size: 6787 MluI A'CGCG_T Cuts at: 6663 6663 Size: 6787 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2021 3010 3357 5901 6085 1305 Size: 716 989 347 2544 184 2007 Fragments arranged by size: 2544 2007 989 716 347 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3471 10 Size: 54 1425 343 1639 3326 Fragments arranged by size: 3326 1639 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2115 4745 5104 5263 518 Size: 648 949 2630 359 159 2042 Fragments arranged by size: 2630 2042 949 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2295 2317 3089 3970 4203 4880 6028 6273 Size: 689 22 772 881 233 677 1148 245 Cuts at: 6273 1606 Size: 2120 Fragments arranged by size: 2120 1148 881 772 689 677 245 233 22 NcoI C'CATG_G Cuts at: 513 1065 3267 3741 513 Size: 552 2202 474 3559 Fragments arranged by size: 3559 2202 552 474 NdeI CA'TA_TG Cuts at: 387 387 Size: 6787 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6787 NotI GC'GGCC_GC Cuts at: 4234 4234 Size: 6787 NspI r_CATG'y Cuts at: 1132 1229 3424 4145 5690 1132 Size: 97 2195 721 1545 2229 Fragments arranged by size: 2229 2195 1545 721 97 PasI CC'CwG_GG Cuts at: 1348 2969 3181 1348 Size: 1621 212 4954 Fragments arranged by size: 4954 1621 212 PciI A'CATG_T Cuts at: 5686 5686 Size: 6787 PflMI CCAn_nnn'nTGG Cuts at: 1108 3029 1108 Size: 1921 4866 PmeI GTTT'AAAC Cuts at: 4091 4091 Size: 6787 PmlI CAC'GTG Cuts at: 4075 4075 Size: 6787 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4856 4888 6369 6401 4856 Size: 32 1481 32 5242 Fragments arranged by size: 5242 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2497 2889 2912 2497 Size: 392 23 6372 Fragments arranged by size: 6372 392 23 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6787 PsiI TTA'TAA Cuts at: 4357 4357 Size: 6787 PspOMI G'GGCC_C Cuts at: 2635 2635 Size: 6787 PspXI vC'TCGA_Gb Cuts at: 2193 2610 2193 Size: 417 6370 PstI C_TGCA'G Cuts at: 830 3088 3109 3234 4139 830 Size: 2258 21 125 905 3478 Fragments arranged by size: 3478 2258 905 125 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6787 PvuII CAG'CTG Cuts at: 2317 3089 3970 2317 Size: 772 881 5134 Fragments arranged by size: 5134 881 772 SacI G_AGCT'C Cuts at: 2732 2732 Size: 6787 SalI G'TCGA_C Cuts at: 1936 4129 1936 Size: 2193 4594 SanDI GG'GwC_CC Cuts at: 2889 2889 Size: 6787 SapI GCTCTTCn'nnn_ Cuts at: 2142 3615 2142 Size: 1473 5314 SbfI CC_TGCA'GG Cuts at: 4139 4139 Size: 6787 ScaI AGT'ACT Cuts at: 5035 6747 5035 Size: 1712 5075 SfcI C'TryA_G Cuts at: 826 1045 3084 3105 3230 4058 4135 5270 5951 Size: 219 2039 21 125 828 77 1135 681 Cuts at: 5951 6142 826 Size: 191 1471 Fragments arranged by size: 2039 1471 1135 828 681 219 191 125 77 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6787 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6787 SmaI CCC'GGG Cuts at: 1872 2600 2640 2677 4114 1872 Size: 728 40 37 1437 4545 Fragments arranged by size: 4545 1437 728 40 37 SmlI C'TyrA_G Cuts at: 820 1017 1943 2193 2610 3506 4891 5792 6054 Size: 197 926 250 417 896 1385 901 262 Cuts at: 6054 820 Size: 1553 Fragments arranged by size: 1553 1385 926 901 896 417 262 250 197 SnaBI TAC'GTA Cuts at: 493 4578 493 Size: 4085 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6787 SphI G_CATG'C Cuts at: 1132 4145 1132 Size: 3013 3774 SrfI GCCC'GGGC Cuts at: 2600 2600 Size: 6787 SspI AAT'ATT Cuts at: 5 52 4711 5 Size: 47 4659 2081 Fragments arranged by size: 4659 2081 47 StuI AGG'CCT Cuts at: 1517 2828 3702 1517 Size: 1311 874 4602 Fragments arranged by size: 4602 1311 874 StyI C'CwwG_G Cuts at: 513 1065 3252 3267 3741 3984 4226 513 Size: 552 2187 15 474 243 242 3074 Fragments arranged by size: 3074 2187 552 474 243 242 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3060 3173 4814 4831 4984 5169 5614 Size: 1255 1480 113 1641 17 153 185 445 Cuts at: 5614 325 Size: 1498 Fragments arranged by size: 1641 1498 1480 1255 445 185 153 113 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3681 5033 6745 Size: 274 80 33 51 1443 1704 1352 1712 Cuts at: 6745 96 Size: 138 Fragments arranged by size: 1712 1704 1443 1352 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2226 2258 2271 2303 6747 6779 2226 Size: 32 13 32 4444 32 2234 Fragments arranged by size: 4444 2234 32 32 32 13 Tth111I GACn'n_nGTC Cuts at: 1316 2910 1316 Size: 1594 5193 XbaI T'CTAG_A Cuts at: 4123 4123 Size: 6787 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2383 2383 Size: 6787 XhoI C'TCGA_G Cuts at: 1943 2193 2610 1943 Size: 250 417 6120 Fragments arranged by size: 6120 417 250 XmaI C'CCGG_G Cuts at: 1870 2598 2638 2675 4112 1870 Size: 728 40 37 1437 4545 Fragments arranged by size: 4545 1437 728 40 37 XmnI GAAnn'nnTTC Cuts at: 3536 4916 3536 Size: 1380 5407 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4595 276 Size: 53 83 186 936 3061 2468 Fragments arranged by size: 3061 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PsrI RsrII SacII SexAI SfiI SfoI SwaI