Restriction map for
pFN21AA0982
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# NO CUT ENZYMES ( 130 enzymes) #
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Acc65I AccI AccIII AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbvCI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 933 GACGTC GACGT^C
AclI 263 AACGTT AA^CGTT
AcyI 933 GACGTC G[GA]^CG[CT]C
AflIII 122 ACATGT A^C[GA][CT]GT
AlwNI 358 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1080 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 514 CTCGGG C^[CT]CG[GA]G
BalI 491 TGGCCA TGG^CCA
BbrPI 592 CACGTG CAC^GTG
BcgI 16 GCAAGTTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 933 GACGTC G[GA]^CG[CT]C
BsaWI 905 ACCGGA [AT]^CCGG[AT]
BseRI 1020 CTCCTC GAGGAG(10/8)
BsmBI 931 GAGACG CGTCTC(1/5)
BsoBI 514 CTCGGG C^[CT]CG[GA]G
BspLU11I 122 ACATGT A^CATGT
BsrFI 1430 ACCGGC [GA]^CCGG[CT]
BstEII 1014 GGTGACC G^GT[ACGT]ACC
BstPI 1014 GGTGACC G^GT[ACGT]ACC
BstYI 1195 AGATCC [GA]^GATC[CT]
Cfr10I 1430 ACCGGC [GA]^CCGG[CT]
DraII 1058 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1127 CCGTGG C^C[GA][CT]GG
EcoO109I 1058 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1014 GGTGACC G^GT[ACGT]ACC
Hin1I 933 GACGTC G[GA]^CG[CT]C
HincII 975 GTTGAC GT[CT]^[GA]AC
HindII 975 GTTGAC GT[CT]^[GA]AC
MflI 1195 AGATCC [GA]^GATC[CT]
MluNI 491 TGGCCA TGG^CCA
MscI 491 TGGCCA TGG^CCA
NspI 122 ACATGT [GA]CATG^[CT]
PmaCI 592 CACGTG CAC^GTG
PmlI 592 CACGTG CAC^GTG
PpuMI 1058 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 263 AACGTT AA^CGTT
PvuII 691 CAGCTG CAG^CTG
SacI 940 GAGCTC GAGCT^C
SmlI 829 CTCAAG C^T[CT][GA]AG
SnaBI 236 TACGTA TAC^GTA
SpeI 721 ACTAGT A^CTAGT
TfiI 139 GATTC G^A[AT]TC
XhoII 1195 AGATCC [GA]^GATC[CT]
XmnI 1080 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 1196 GATCC GGATC(4/5)
1238 GGATC
AvaII 903 GGACC G^G[AT]CC
1059 GGACC
BbsI 366 GAAGAC GAAGAC(2/6)
1390 GAAGAC
BcnI 465 CCGGG CC^[GC]GG
1337 CCCGG
BfaI 574 CTAG C^TAG
722 CTAG
BpuAI 366 GAAGAC GAAGAC(2/6)
1390 GAAGAC
BsaAI 236 TACGTA [CT]AC^GT[GA]
592 CACGTG
BsgI 987 CTGCAC GTGCAG(16/14)
688 GTGCAG
BsrDI 1203 CATTGC GCAATG(2/0)
1466 CATTGC
BssSI 442 CTCGTG CACGAG(-5/-1)
1009 CTCGTG
DraIII 1072 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
1396 CACCCCGTG
HgaI 978 GACGC GACGC(5/10)
812 GCGTC
MaeI 574 CTAG C^TAG
722 CTAG
MspA1I 691 CAGCTG C[AC]G^C[GT]G
1003 CAGCGG
NciI 465 CCGGG CC^[GC]GG
1337 CCCGG
PflMI 840 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1439 CCATTGGTTGG
PleI 796 GACTC GAGTC(4/5)
1222 GACTC
SfaNI 473 GCATC GCATC(5/9)
523 GCATC
Van91I 840 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1439 CCATTGGTTGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI BanII BbvI BglI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I