(Circular) (Six-base) MAPSORT of: pFN21AA0982.seq Check: 5624 from: 1 to: 6187 pFN21AA0982 6187 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2942 3997 278 Size: 53 83 186 936 1406 1055 2468 Fragments arranged by size: 2468 1406 1055 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 2269 4314 4687 1563 Size: 706 2045 373 3063 Fragments arranged by size: 3063 2045 706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2301 2390 4250 5634 5902 1632 Size: 669 89 1860 1384 268 1917 Fragments arranged by size: 1917 1860 1384 669 268 89 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 2127 5086 6063 1203 Size: 924 2959 977 1327 Fragments arranged by size: 2959 1327 977 924 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6187 AlwNI CAG_nnn'CTG Cuts at: 2368 3930 5502 2368 Size: 1562 1572 3053 Fragments arranged by size: 3053 1572 1562 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 2207 2611 2621 3496 3722 3874 5983 1493 Size: 714 404 10 875 226 152 2109 1697 Fragments arranged by size: 2109 1697 875 714 404 226 152 10 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 2519 3512 1870 Size: 73 576 993 4545 Fragments arranged by size: 4545 993 576 73 BamHI G'GATC_C Cuts at: 3517 3884 3517 Size: 367 5820 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3508 4963 618 Size: 325 217 627 1721 1455 1842 Fragments arranged by size: 1842 1721 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2606 2775 2949 3506 1391 Size: 1215 169 174 557 4072 Fragments arranged by size: 4072 1215 557 174 169 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2378 3402 928 Size: 971 479 1024 3713 Fragments arranged by size: 3713 1024 971 479 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2010 2021 2044 4378 4412 1903 Size: 34 50 23 11 23 2334 34 3678 Fragments arranged by size: 3678 2334 50 34 34 23 23 11 BciVI GTATCCnnnnn_n' Cuts at: 4079 5289 4079 Size: 1210 4977 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2601 2847 3357 Size: 107 122 71 631 605 929 246 510 Cuts at: 3357 4798 136 Size: 1441 1525 Fragments arranged by size: 1525 1441 929 631 605 510 246 122 107 71 BglII A'GATC_T Cuts at: 6182 6182 Size: 6187 BlpI GC'TnA_GC Cuts at: 3604 3604 Size: 6187 Bme1580I G_kGCm'C Cuts at: 1790 2970 4248 5404 1790 Size: 1180 1278 1156 2573 Fragments arranged by size: 2573 1278 1180 1156 BmgBI CAC'GTC Cuts at: 1318 2932 1318 Size: 1614 4573 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3918 4876 442 Size: 416 53 83 399 24 2501 958 1753 Fragments arranged by size: 2501 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2392 2679 2701 4847 1239 Size: 1153 287 22 2146 2579 Fragments arranged by size: 2579 2146 1153 287 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2819 4312 5177 5475 2819 Size: 1493 865 298 3531 Fragments arranged by size: 3531 1493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4850 882 Size: 617 3351 2219 Fragments arranged by size: 3351 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2243 2599 3978 493 Size: 711 1039 356 1379 2702 Fragments arranged by size: 2702 1379 1039 711 356 BsaBI GATnn'nnATC Cuts at: 6181 6181 Size: 6187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2939 3994 4376 5011 Size: 53 83 186 936 1406 1055 382 635 Cuts at: 5011 275 Size: 1451 Fragments arranged by size: 1451 1406 1055 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2910 4620 5018 5292 5439 5850 2910 Size: 1710 398 274 147 411 3247 Fragments arranged by size: 3247 1710 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3129 3159 1178 Size: 30 165 30 1726 30 4206 Fragments arranged by size: 4206 1726 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3016 1186 Size: 1830 4357 BseYI C'CCAG_C Cuts at: 2709 3006 5390 5886 2709 Size: 297 2384 496 3010 Fragments arranged by size: 3010 2384 496 297 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2714 2977 1163 Size: 573 978 263 4373 Fragments arranged by size: 4373 978 573 263 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3637 4398 5426 1540 Size: 275 186 1636 761 1028 2301 Fragments arranged by size: 2301 1636 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2520 2759 2949 4248 4333 5404 2520 Size: 239 190 1299 85 1071 3303 Fragments arranged by size: 3303 1299 1071 239 190 85 BsmI GAATG_Cn' Cuts at: 3698 3791 3698 Size: 93 6094 BsmBI CGTCTCn'nnnn_ Cuts at: 2930 2930 Size: 6187 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2520 2606 2759 2775 2949 2970 3506 Size: 399 730 86 153 16 174 21 536 Cuts at: 3506 4248 4333 5404 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 730 536 399 174 153 86 85 21 16 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 4076 5806 4076 Size: 1730 4457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3542 844 Size: 2698 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4074 1394 Size: 429 2251 3507 Fragments arranged by size: 3507 2251 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3207 3470 4682 4856 65 Size: 1183 1959 263 1212 174 1396 Fragments arranged by size: 1959 1396 1212 1183 263 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3435 4831 5018 1700 Size: 110 1625 1396 187 2869 Fragments arranged by size: 2869 1625 1396 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6187 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6187 BssSI C'ACGA_G Cuts at: 2447 3014 4247 5259 2447 Size: 567 1233 1012 3375 Fragments arranged by size: 3375 1233 1012 567 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6187 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 3019 1184 Size: 1835 4352 BstYI r'GATC_y Cuts at: 1972 3200 3517 3884 4269 4286 5738 5824 5836 Size: 1228 317 367 385 17 1452 86 12 Cuts at: 5836 6182 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1228 385 367 346 317 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3132 513 Size: 552 2067 3568 Fragments arranged by size: 3568 2067 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2842 3174 487 Size: 683 56 1616 332 3500 Fragments arranged by size: 3500 1616 683 332 56 BtsI GCAGTG_nn' Cuts at: 2649 2986 3703 4497 4517 2649 Size: 337 717 794 20 4319 Fragments arranged by size: 4319 794 717 337 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DraIII CAC_nnn'GTG Cuts at: 3082 3406 3082 Size: 324 5863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2464 2496 3259 Size: 54 1425 50 275 18 634 32 763 Cuts at: 3259 3634 4523 6152 8 Size: 375 889 1629 43 Fragments arranged by size: 1629 1425 889 763 634 375 275 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 3634 1537 Size: 275 1822 4090 Fragments arranged by size: 4090 1822 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4117 1353 Size: 2764 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3035 4774 5148 5294 387 Size: 1064 138 1446 1739 374 146 1280 Fragments arranged by size: 1739 1446 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2947 2947 Size: 6187 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 2390 2392 2679 2701 4250 4847 Size: 393 669 89 2 287 22 1549 597 Cuts at: 4847 5634 5902 1239 Size: 787 268 1524 Fragments arranged by size: 1549 1524 787 669 597 393 287 268 89 22 2 EcoO109I rG'GnC_Cy Cuts at: 3064 3064 Size: 6187 EcoRI G'AATT_C Cuts at: 3496 3874 3496 Size: 378 5809 FspI TGC'GCA Cuts at: 4693 4693 Size: 6187 HaeII r_GCGC'y Cuts at: 1678 5334 1678 Size: 3656 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4828 4860 4902 4934 1373 Size: 32 135 32 3256 32 42 32 2626 Fragments arranged by size: 3256 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2982 3531 3777 669 Size: 988 281 1044 549 246 3079 Fragments arranged by size: 3079 1044 988 549 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6187 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2889 3431 5301 5485 1305 Size: 1584 542 1870 184 2007 Fragments arranged by size: 2007 1870 1584 542 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2498 10 Size: 54 1425 343 666 3699 Fragments arranged by size: 3699 1425 666 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4145 4504 4663 518 Size: 648 2979 359 159 2042 Fragments arranged by size: 2979 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2698 3010 3603 4280 5428 5673 1606 Size: 1092 312 593 677 1148 245 2120 Fragments arranged by size: 2120 1148 1092 677 593 312 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5635 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 2131 3545 5090 1132 Size: 97 902 1414 1545 2229 Fragments arranged by size: 2229 1545 1414 902 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6187 PciI A'CATG_T Cuts at: 2127 5086 2127 Size: 2959 3228 PflMI CCAn_nnn'nTGG Cuts at: 1108 2851 3450 1108 Size: 1743 599 3845 Fragments arranged by size: 3845 1743 599 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PmlI CAC'GTG Cuts at: 2599 2599 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4256 4288 5769 5801 4256 Size: 32 1481 32 4642 Fragments arranged by size: 4642 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3064 3064 Size: 6187 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6187 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PstI C_TGCA'G Cuts at: 830 3539 830 Size: 2709 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 PvuII CAG'CTG Cuts at: 2698 2698 Size: 6187 SacI G_AGCT'C Cuts at: 2949 2949 Size: 6187 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SanDI GG'GwC_CC Cuts at: 3064 3064 Size: 6187 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SfcI C'TryA_G Cuts at: 826 1045 3535 4670 5351 5542 826 Size: 219 2490 1135 681 191 1471 Fragments arranged by size: 2490 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 3514 1872 Size: 1642 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2834 4291 5192 5454 820 Size: 197 926 891 1457 901 262 1553 Fragments arranged by size: 1553 1457 926 901 891 262 197 SnaBI TAC'GTA Cuts at: 493 2243 3978 493 Size: 1750 1735 2702 Fragments arranged by size: 2702 1750 1735 SpeI A'CTAG_T Cuts at: 152 2726 152 Size: 2574 3613 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6187 StyI C'CwwG_G Cuts at: 513 1065 3626 513 Size: 552 2561 3074 Fragments arranged by size: 3074 2561 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3020 4214 4231 4384 4569 5014 325 Size: 1255 1440 1194 17 153 185 445 1498 Fragments arranged by size: 1498 1440 1255 1194 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2171 2457 4433 Size: 274 80 33 51 1443 194 286 1976 Cuts at: 4433 6145 96 Size: 1712 138 Fragments arranged by size: 1976 1712 1443 286 274 194 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3129 3161 6147 6179 3129 Size: 32 2986 32 3137 Fragments arranged by size: 3137 2986 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6187 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 3512 1870 Size: 1642 4545 XmnI GAAnn'nnTTC Cuts at: 3089 4316 3089 Size: 1227 4960 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2940 3995 276 Size: 53 83 186 936 1406 1055 2468 Fragments arranged by size: 2468 1406 1055 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI