Restriction map for
pFN21AA0982
(Linear) (Six-base) MAPSORT of: KIAA0982.seq Check: 3992 from: 1 to: 1482
KIAA0982.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AatII G_ACGT'C
Cuts at: 0 937 1482
Size: 937 545
AclI AA'CG_TT
Cuts at: 0 264 1482
Size: 264 1218
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 385 1482
Size: 296 89 1097
Fragments arranged by size:
1097 296 89
AflIII A'CryG_T
Cuts at: 0 122 1482
Size: 122 1360
AlwNI CAG_nnn'CTG
Cuts at: 0 363 1482
Size: 363 1119
ApoI r'AATT_y
Cuts at: 0 202 606 616 1482
Size: 202 404 10 866
Fragments arranged by size:
866 404 202 10
AvaI C'yCGr_G
Cuts at: 0 514 1482
Size: 514 968
BanII G_rGCy'C
Cuts at: 0 601 770 944 1482
Size: 601 169 174 538
Fragments arranged by size:
601 538 174 169
BbsI GAAGACnn'nnnn_
Cuts at: 0 373 1397 1482
Size: 373 1024 85
Fragments arranged by size:
1024 373 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5 39 1482
Size: 5 34 1443
Fragments arranged by size:
1443 34 5
BglI GCCn_nnn'nGGC
Cuts at: 0 596 842 1352 1482
Size: 596 246 510 130
Fragments arranged by size:
596 510 246 130
Bme1580I G_kGCm'C
Cuts at: 0 965 1482
Size: 965 517
BmgBI CAC'GTC
Cuts at: 0 927 1482
Size: 927 555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 674 696 1482
Size: 387 287 22 786
Fragments arranged by size:
786 387 287 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1482
Size: 814 668
BsaAI yAC'GTr
Cuts at: 0 238 594 1482
Size: 238 356 888
Fragments arranged by size:
888 356 238
BsaHI Gr'CG_yC
Cuts at: 0 934 1482
Size: 934 548
BsaWI w'CCGG_w
Cuts at: 0 905 1482
Size: 905 577
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1124 1154 1482
Size: 1124 30 328
Fragments arranged by size:
1124 328 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1011 1482
Size: 1011 471
BseYI C'CCAG_C
Cuts at: 0 704 1001 1482
Size: 704 297 481
Fragments arranged by size:
704 481 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 972 1482
Size: 709 263 510
Fragments arranged by size:
709 510 263
BsiHKAI G_wGCw'C
Cuts at: 0 515 754 944 1482
Size: 515 239 190 538
Fragments arranged by size:
538 515 239 190
BsmBI CGTCTCn'nnnn_
Cuts at: 0 925 1482
Size: 925 557
Bsp1286I G_dGCh'C
Cuts at: 0 515 601 754 770 944 965 1482
Size: 515 86 153 16 174 21 517
Fragments arranged by size:
517 515 174 153 86 21 16
BsrDI GCAATG_nn'
Cuts at: 0 1202 1465 1482
Size: 1202 263 17
Fragments arranged by size:
1202 263 17
BsrFI r'CCGG_y
Cuts at: 0 1430 1482
Size: 1430 52
BssSI C'ACGA_G
Cuts at: 0 442 1009 1482
Size: 442 567 473
Fragments arranged by size:
567 473 442
BstEII G'GTnAC_C
Cuts at: 0 1014 1482
Size: 1014 468
BstYI r'GATC_y
Cuts at: 0 1195 1482
Size: 1195 287
BtgI C'CryG_G
Cuts at: 0 1127 1482
Size: 1127 355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 837 1169 1482
Size: 837 332 313
Fragments arranged by size:
837 332 313
BtsI GCAGTG_nn'
Cuts at: 0 644 981 1482
Size: 644 337 501
Fragments arranged by size:
644 501 337
DraIII CAC_nnn'GTG
Cuts at: 0 1077 1401 1482
Size: 1077 324 81
Fragments arranged by size:
1077 324 81
EaeI y'GGCC_r
Cuts at: 0 459 491 1254 1482
Size: 459 32 763 228
Fragments arranged by size:
763 459 228 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1030 1482
Size: 1030 452
EcoICRI GAG'CTC
Cuts at: 0 942 1482
Size: 942 540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 385 387 674 696 1482
Size: 296 89 2 287 22 786
Fragments arranged by size:
786 296 287 89 22 2
EcoO109I rG'GnC_Cy
Cuts at: 0 1059 1482
Size: 1059 423
HincII GTy'rAC
Cuts at: 0 977 1482
Size: 977 505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 1426 1482
Size: 884 542 56
Fragments arranged by size:
884 542 56
MscI TGG'CCA
Cuts at: 0 493 1482
Size: 493 989
MspA1I CmG'CkG
Cuts at: 0 693 1005 1482
Size: 693 312 477
Fragments arranged by size:
693 477 312
NspI r_CATG'y
Cuts at: 0 126 1482
Size: 126 1356
PciI A'CATG_T
Cuts at: 0 122 1482
Size: 122 1360
PflMI CCAn_nnn'nTGG
Cuts at: 0 846 1445 1482
Size: 846 599 37
Fragments arranged by size:
846 599 37
PmlI CAC'GTG
Cuts at: 0 594 1482
Size: 594 888
PpuMI rG'GwC_Cy
Cuts at: 0 1059 1482
Size: 1059 423
PvuII CAG'CTG
Cuts at: 0 693 1482
Size: 693 789
SacI G_AGCT'C
Cuts at: 0 944 1482
Size: 944 538
SanDI GG'GwC_CC
Cuts at: 0 1059 1482
Size: 1059 423
SmlI C'TyrA_G
Cuts at: 0 829 1482
Size: 829 653
SnaBI TAC'GTA
Cuts at: 0 238 1482
Size: 238 1244
SpeI A'CTAG_T
Cuts at: 0 721 1482
Size: 721 761
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1015 1482
Size: 1015 467
TatI w'GTAC_w
Cuts at: 0 166 452 1482
Size: 166 286 1030
Fragments arranged by size:
1030 286 166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1124 1156 1482
Size: 1124 32 326
Fragments arranged by size:
1124 326 32
XmnI GAAnn'nnTTC
Cuts at: 0 1084 1482
Size: 1084 398
ZraI GAC'GTC
Cuts at: 0 935 1482
Size: 935 547
Enzymes that do cut:
AatII AclI AcuI AflIII AlwNI ApoI AvaI BanII
BbsI BcgI BglI Bme1580I BmgBI BpmI BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BsrDI BsrFI BssSI BstEII BstYI BtgI BtgZI
BtsI DraIII EaeI EciI EcoICRI Eco57MI EcoO109I HincII
MmeI MscI MspA1I NspI PciI PflMI PmlI PpuMI
PvuII SacI SanDI SmlI SnaBI SpeI TaqII TatI
TstI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BplI Bpu10I BsaI BsaBI
BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrGI
BssHII BstAPI BstBI BstXI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EagI EarI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SphI SrfI
SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI
XmaI