(Circular) (Six-base) MAPSORT of: pFN21AA0973.seq Check: 3519 from: 1 to: 9415 pFN21AA0973 9415 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2916 2916 Size: 9415 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6162 7225 278 Size: 53 83 186 936 4626 1063 2468 Fragments arranged by size: 4626 2468 1063 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2547 2733 3168 4962 6758 1937 Size: 610 186 435 1794 1796 4594 Fragments arranged by size: 4594 1796 1794 610 435 186 Acc65I G'GTAC_C Cuts at: 6304 6736 6304 Size: 432 8983 AclI AA'CG_TT Cuts at: 1563 7542 7915 1563 Size: 5979 373 3063 Fragments arranged by size: 5979 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3413 4120 4408 6672 7478 8862 9130 1632 Size: 1781 707 288 2264 806 1384 268 1917 Fragments arranged by size: 2264 1917 1781 1384 806 707 288 268 AfeI AGC'GCT Cuts at: 3200 3408 4919 6440 3200 Size: 208 1511 1521 6175 Fragments arranged by size: 6175 1521 1511 208 AflII C'TTAA_G Cuts at: 820 1017 5126 820 Size: 197 4109 5109 Fragments arranged by size: 5109 4109 197 AflIII A'CryG_T Cuts at: 1203 8314 9291 1203 Size: 7111 977 1327 Fragments arranged by size: 7111 1327 977 AgeI A'CCGG_T Cuts at: 8246 8246 Size: 9415 AhdI GACnn_n'nnGTC Cuts at: 8144 8144 Size: 9415 AleI CACnn'nnGTG Cuts at: 1166 5069 1166 Size: 3903 5512 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3219 5940 1184 Size: 2035 2721 4659 Fragments arranged by size: 4659 2721 2035 AlwNI CAG_nnn'CTG Cuts at: 4751 6361 7158 8730 4751 Size: 1610 797 1572 5436 Fragments arranged by size: 5436 1610 1572 797 ApaI G_GGCC'C Cuts at: 4238 4516 4238 Size: 278 9137 ApaLI G'TGCA_C Cuts at: 3485 6109 6558 7472 8628 3485 Size: 2624 449 914 1156 4272 Fragments arranged by size: 4272 2624 1156 914 449 ApoI r'AATT_y Cuts at: 1493 2882 6724 6950 7102 9211 1493 Size: 1389 3842 226 152 2109 1697 Fragments arranged by size: 3842 2109 1697 1389 226 152 AscI GG'CGCG_CC Cuts at: 5967 5967 Size: 9415 AseI AT'TA_AT Cuts at: 160 1032 7969 160 Size: 872 6937 1606 Fragments arranged by size: 6937 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9415 AvaI C'yCGr_G Cuts at: 1870 1943 2495 2688 2891 3621 3671 3824 4238 Size: 73 552 193 203 730 50 153 414 Cuts at: 4238 4921 5377 5485 5864 6044 6091 6332 6416 Size: 683 456 108 379 180 47 241 84 Cuts at: 6416 6426 6607 6706 6740 1870 Size: 10 181 99 34 4545 Fragments arranged by size: 4545 730 683 552 456 414 379 241 203 193 181 180 153 108 99 84 73 50 47 34 10 AvrII C'CTAG_G Cuts at: 2173 5452 2173 Size: 3279 6136 BamHI G'GATC_C Cuts at: 4496 6745 7112 4496 Size: 2249 367 6799 Fragments arranged by size: 6799 2249 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 3152 3180 3336 4382 Size: 325 217 627 516 849 28 156 1046 Cuts at: 4382 4541 4668 5456 6050 6098 6304 6349 6403 Size: 159 127 788 594 48 206 45 54 Cuts at: 6403 6611 6642 6736 8191 618 Size: 208 31 94 1455 1842 Fragments arranged by size: 1842 1455 1046 849 788 627 594 516 325 217 208 206 159 156 127 94 54 48 45 31 28 BanII G_rGCy'C Cuts at: 1391 2394 3573 4098 4182 4238 4313 4416 4516 Size: 1003 1179 525 84 56 75 103 100 Cuts at: 4516 4896 5384 5769 6245 6525 6608 6734 1391 Size: 380 488 385 476 280 83 126 4072 Fragments arranged by size: 4072 1179 1003 525 488 476 385 380 280 126 103 100 84 83 75 56 BbeI G_GCGC'C Cuts at: 3340 4386 5460 6102 6407 3340 Size: 1046 1074 642 305 6348 Fragments arranged by size: 6348 1074 1046 642 305 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2960 4301 928 Size: 971 1061 1341 6042 Fragments arranged by size: 6042 1341 1061 971 BbvCI CC'TCA_GC Cuts at: 2489 3971 4744 6354 2489 Size: 1482 773 1610 5550 Fragments arranged by size: 5550 1610 1482 773 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5299 5333 5510 5544 7606 7640 1903 Size: 34 3362 34 177 34 2062 34 3678 Fragments arranged by size: 3678 3362 2062 177 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7307 8517 7307 Size: 1210 8205 BclI T'GATC_A Cuts at: 1548 1548 Size: 9415 BfrBI ATG'CAT Cuts at: 4622 4622 Size: 9415 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2988 4474 5036 Size: 107 122 71 631 605 1316 1486 562 Cuts at: 5036 5997 6233 6328 8026 136 Size: 961 236 95 1698 1525 Fragments arranged by size: 1698 1525 1486 1316 961 631 605 562 236 122 107 95 71 BglII A'GATC_T Cuts at: 9410 9410 Size: 9415 BlpI GC'TnA_GC Cuts at: 2654 6239 6476 6832 2654 Size: 3585 237 356 5237 Fragments arranged by size: 5237 3585 356 237 Bme1580I G_kGCm'C Cuts at: 1790 3489 3762 4238 4516 6113 6296 6417 6562 Size: 1699 273 476 278 1597 183 121 145 Cuts at: 6562 6614 6647 6663 7476 8632 1790 Size: 52 33 16 813 1156 2573 Fragments arranged by size: 2573 1699 1597 1156 813 476 278 273 183 145 121 52 33 16 BmgBI CAC'GTC Cuts at: 1318 2545 3016 1318 Size: 1227 471 7717 Fragments arranged by size: 7717 1227 471 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2310 4184 4728 Size: 416 53 83 399 24 893 1874 544 Cuts at: 4728 5945 6575 7146 8104 442 Size: 1217 630 571 958 1753 Fragments arranged by size: 1874 1753 1217 958 893 630 571 544 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3455 1056 Size: 2399 7016 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4728 6070 6501 4728 Size: 1342 431 7642 Fragments arranged by size: 7642 1342 431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2780 2891 2933 3047 3479 4321 4376 4430 Size: 1541 111 42 114 432 842 55 54 Cuts at: 4430 4531 6040 6170 8075 1239 Size: 101 1509 130 1905 2579 Fragments arranged by size: 2579 1905 1541 1509 842 432 130 114 111 101 55 54 42 Bpu10I CC'TnA_GC Cuts at: 2489 3060 3971 4744 6339 6354 2489 Size: 571 911 773 1595 15 5550 Fragments arranged by size: 5550 1595 911 773 571 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3482 4515 5771 6606 7540 8405 8703 3482 Size: 1033 1256 835 934 865 298 4194 Fragments arranged by size: 4194 1256 1033 934 865 835 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2140 2277 3942 8078 882 Size: 617 641 137 1665 4136 2219 Fragments arranged by size: 4136 2219 1665 641 617 137 BsaAI yAC'GTr Cuts at: 493 1204 4057 5071 7206 493 Size: 711 2853 1014 2135 2702 Fragments arranged by size: 2853 2702 2135 1014 711 BsaBI GATnn'nnATC Cuts at: 9409 9409 Size: 9415 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2263 2778 3337 3618 Size: 53 83 186 936 730 515 559 281 Cuts at: 3618 4383 5457 5516 5651 5945 6099 6159 6404 Size: 765 1074 59 135 294 154 60 245 Cuts at: 6404 7222 7604 8239 275 Size: 818 382 635 1451 Fragments arranged by size: 1451 1074 936 818 765 730 635 559 515 382 294 281 245 186 154 135 83 60 59 53 BsaWI w'CCGG_w Cuts at: 4315 5289 7848 8246 8520 8667 9078 4315 Size: 974 2559 398 274 147 411 4652 Fragments arranged by size: 4652 2559 974 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2387 2417 4046 4076 4151 Size: 30 165 30 984 30 1629 30 75 Cuts at: 4151 4181 1178 Size: 30 6412 Fragments arranged by size: 6412 1629 984 165 75 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2233 2332 2500 2600 2909 3797 3851 4103 Size: 1047 99 168 100 309 888 54 252 Cuts at: 4103 4106 4256 4538 4846 5774 6476 1186 Size: 3 150 282 308 928 702 4125 Fragments arranged by size: 4125 1047 928 888 702 309 308 282 252 168 150 100 99 54 3 BseYI C'CCAG_C Cuts at: 2461 2986 4270 4415 4478 4862 5026 5051 5479 Size: 525 1284 145 63 384 164 25 428 Cuts at: 5479 5534 5812 6441 6544 6563 6664 8618 9114 Size: 55 278 629 103 19 101 1954 496 Cuts at: 9114 2461 Size: 2762 Fragments arranged by size: 2762 1954 1284 629 525 496 428 384 278 164 145 103 101 63 55 25 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5528 5705 5816 6151 6206 6269 6574 Size: 573 3792 177 111 335 55 63 305 Cuts at: 6574 6591 1163 Size: 17 3987 Fragments arranged by size: 3987 3792 573 335 305 177 111 63 55 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2922 5601 5805 6865 7626 8654 Size: 275 186 921 2679 204 1060 761 1028 Cuts at: 8654 1540 Size: 2301 Fragments arranged by size: 2679 2301 1060 1028 921 761 275 204 186 BsiHKAI G_wGCw'C Cuts at: 2394 2423 2655 3489 4229 5492 6113 6562 7476 Size: 29 232 834 740 1263 621 449 914 Cuts at: 7476 7561 8632 2394 Size: 85 1071 3177 Fragments arranged by size: 3177 1263 1071 914 834 740 621 449 232 85 29 BsmI GAATG_Cn' Cuts at: 6926 7019 6926 Size: 93 9322 BsmBI CGTCTCn'nnnn_ Cuts at: 2332 3437 5936 2332 Size: 1105 2499 5811 Fragments arranged by size: 5811 2499 1105 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2394 2423 2655 3489 3573 3762 4098 Size: 399 604 29 232 834 84 189 336 Cuts at: 4098 4182 4229 4238 4313 4416 4516 4896 5384 Size: 84 47 9 75 103 100 380 488 Cuts at: 5384 5492 5769 6113 6245 6296 6417 6525 6562 Size: 108 277 344 132 51 121 108 37 Cuts at: 6562 6608 6614 6647 6663 6734 7476 7561 8632 Size: 46 6 33 16 71 742 85 1071 Cuts at: 8632 1391 Size: 2174 Fragments arranged by size: 2174 1071 834 742 604 488 399 380 344 336 277 232 189 132 121 108 108 103 100 85 84 84 75 71 51 47 46 37 33 29 16 9 6 BspEI T'CCGG_A Cuts at: 4315 5289 9078 4315 Size: 974 3789 4652 Fragments arranged by size: 4652 3789 974 BspHI T'CATG_A Cuts at: 3573 4764 7304 9034 3573 Size: 1191 2540 1730 3954 Fragments arranged by size: 3954 2540 1730 1191 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2916 5322 5968 6770 844 Size: 2072 2406 646 802 3489 Fragments arranged by size: 3489 2406 2072 802 646 BsrBI CCG'CTC Cuts at: 1394 1823 2478 4038 4899 5331 5352 5430 5580 Size: 429 655 1560 861 432 21 78 150 Cuts at: 5580 5782 6154 6480 7302 1394 Size: 202 372 326 822 3507 Fragments arranged by size: 3507 1560 861 822 655 432 429 372 326 202 150 78 21 BsrDI GCAATG_nn' Cuts at: 65 1248 7910 8084 65 Size: 1183 6662 174 1396 Fragments arranged by size: 6662 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2265 4253 5401 5600 6267 8059 8246 Size: 110 455 1988 1148 199 667 1792 187 Cuts at: 8246 1700 Size: 2869 Fragments arranged by size: 2869 1988 1792 1148 667 455 199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9415 BssHII G'CGCG_C Cuts at: 1925 5405 5844 5967 1925 Size: 3480 439 123 5373 Fragments arranged by size: 5373 3480 439 123 BssSI C'ACGA_G Cuts at: 4175 6257 7475 8487 4175 Size: 2082 1218 1012 5103 Fragments arranged by size: 5103 2082 1218 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3318 5398 794 Size: 2524 2080 4811 Fragments arranged by size: 4811 2524 2080 BstBI TT'CG_AA Cuts at: 5166 8219 5166 Size: 3053 6362 BstEII G'GTnAC_C Cuts at: 1184 2168 4589 1184 Size: 984 2421 6010 Fragments arranged by size: 6010 2421 984 BstXI CCAn_nnnn'nTGG Cuts at: 3711 3892 4354 4648 5075 6223 3711 Size: 181 462 294 427 1148 6903 Fragments arranged by size: 6903 1148 462 427 294 181 BstYI r'GATC_y Cuts at: 1972 3249 4496 6745 7112 7497 7514 8966 9052 Size: 1277 1247 2249 367 385 17 1452 86 Cuts at: 9052 9064 9410 1972 Size: 12 346 1977 Fragments arranged by size: 2249 1977 1452 1277 1247 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5106 5106 Size: 9415 BtgI C'CryG_G Cuts at: 513 1065 2379 2727 3525 4098 4491 5899 513 Size: 552 1314 348 798 573 393 1408 4029 Fragments arranged by size: 4029 1408 1314 798 573 552 393 348 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3649 5552 5602 5712 487 Size: 683 56 2423 1903 50 110 4190 Fragments arranged by size: 4190 2423 1903 683 110 56 50 BtsI GCAGTG_nn' Cuts at: 2571 4749 4782 4943 5154 5663 6562 6574 6579 Size: 2178 33 161 211 509 899 12 5 Cuts at: 6579 6931 7725 7745 2571 Size: 352 794 20 4241 Fragments arranged by size: 4241 2178 899 794 509 352 211 161 33 20 12 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9415 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9380 DraI TTT'AAA Cuts at: 6719 6719 Size: 9415 DraIII CAC_nnn'GTG Cuts at: 2950 4057 4949 5680 2950 Size: 1107 892 731 6685 Fragments arranged by size: 6685 1107 892 731 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4979 8422 809 Size: 507 3663 3443 1802 Fragments arranged by size: 3663 3443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2449 2640 2919 Size: 54 1425 50 275 18 619 191 279 Cuts at: 2919 4251 5598 5712 5802 6139 6213 6225 6862 Size: 1332 1347 114 90 337 74 12 637 Cuts at: 6862 7751 9380 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1347 1332 889 637 619 337 279 275 191 114 90 74 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2919 5598 5802 6862 1537 Size: 275 1107 2679 204 1060 4090 Fragments arranged by size: 4090 2679 1107 1060 275 204 EarI CTCTTCn'nnn_ Cuts at: 1353 4123 4697 5291 7345 1353 Size: 2770 574 594 2054 3423 Fragments arranged by size: 3423 2770 2054 594 574 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2063 2270 2969 4097 5236 5434 Size: 1064 138 474 207 699 1128 1139 198 Cuts at: 5434 5978 8002 8376 8522 387 Size: 544 2024 374 146 1280 Fragments arranged by size: 2024 1280 1139 1128 1064 699 544 474 374 207 198 146 138 EcoICRI GAG'CTC Cuts at: 2392 2392 Size: 9415 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2780 2891 2933 3047 3413 3479 4120 Size: 393 1148 111 42 114 366 66 641 Cuts at: 4120 4321 4376 4408 4430 4531 6040 6170 6672 Size: 201 55 32 22 101 1509 130 502 Cuts at: 6672 7478 8075 8862 9130 1239 Size: 806 597 787 268 1524 Fragments arranged by size: 1524 1509 1148 806 787 641 597 502 393 366 268 201 130 114 111 101 66 55 42 32 22 EcoNI CCTnn'n_nnAGG Cuts at: 2248 2912 2248 Size: 664 8751 EcoO109I rG'GnC_Cy Cuts at: 3651 3838 4234 4235 4513 5056 5959 6421 3651 Size: 187 396 1 278 543 903 462 6645 Fragments arranged by size: 6645 903 543 462 396 278 187 1 EcoRI G'AATT_C Cuts at: 2882 6724 7102 2882 Size: 3842 378 5195 Fragments arranged by size: 5195 3842 378 EcoRV GAT'ATC Cuts at: 3277 3277 Size: 9415 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3247 4954 3247 Size: 1707 7708 FspI TGC'GCA Cuts at: 2577 4463 5852 7921 2577 Size: 1886 1389 2069 4071 Fragments arranged by size: 4071 2069 1886 1389 HaeII r_GCGC'y Cuts at: 1678 2356 2503 3165 3202 3340 3410 3918 4386 Size: 678 147 662 37 138 70 508 468 Cuts at: 4386 4406 4431 4554 4921 5460 5915 6102 6239 Size: 20 25 123 367 539 455 187 137 Cuts at: 6239 6407 6442 8562 1678 Size: 168 35 2120 2531 Fragments arranged by size: 2531 2120 678 662 539 508 468 455 367 187 168 147 138 137 123 70 37 35 25 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2524 2556 3059 3091 3786 Size: 32 135 32 952 32 503 32 695 Cuts at: 3786 3818 3874 3906 4022 4054 4151 4183 6071 Size: 32 56 32 116 32 97 32 1888 Cuts at: 6071 6103 8056 8088 8130 8162 1373 Size: 32 1953 32 42 32 2626 Fragments arranged by size: 2626 1953 1888 952 695 503 135 116 97 56 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6759 7005 669 Size: 988 281 4821 246 3079 Fragments arranged by size: 4821 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9415 HpaI GTT'AAC Cuts at: 7005 7005 Size: 9415 KasI G'GCGC_C Cuts at: 3336 4382 5456 6098 6403 3336 Size: 1046 1074 642 305 6348 Fragments arranged by size: 6348 1074 1046 642 305 KpnI G_GTAC'C Cuts at: 6308 6740 6308 Size: 432 8983 MfeI C'AATT_G Cuts at: 7014 7014 Size: 9415 MluI A'CGCG_T Cuts at: 9291 9291 Size: 9415 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3075 3987 4509 4860 5958 6429 8529 8713 Size: 1770 912 522 351 1098 471 2100 184 Cuts at: 8713 1305 Size: 2007 Fragments arranged by size: 2100 2007 1770 1098 912 522 471 351 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6215 6227 10 Size: 54 1425 343 4383 12 3198 Fragments arranged by size: 4383 3198 1425 343 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 3353 3474 3524 5069 5073 7373 7732 Size: 648 2187 121 50 1545 4 2300 359 Cuts at: 7732 7891 518 Size: 159 2042 Fragments arranged by size: 2300 2187 2042 1545 648 359 159 121 50 4 MspA1I CmG'CkG Cuts at: 1606 2161 2365 2824 2986 4165 4199 4493 4883 Size: 555 204 459 162 1179 34 294 390 Cuts at: 4883 5623 6831 7508 8656 8901 1606 Size: 740 1208 677 1148 245 2120 Fragments arranged by size: 2120 1208 1179 1148 740 677 555 459 390 294 245 204 162 34 NaeI GCC'GGC Cuts at: 4255 5403 5602 6269 4255 Size: 1148 199 667 7401 Fragments arranged by size: 7401 1148 667 199 NarI GG'CG_CC Cuts at: 3337 4383 5457 6099 6404 3337 Size: 1046 1074 642 305 6348 Fragments arranged by size: 6348 1074 1046 642 305 NcoI C'CATG_G Cuts at: 513 1065 3525 5899 513 Size: 552 2460 2374 4029 Fragments arranged by size: 4029 2460 2374 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9415 NgoMIV G'CCGG_C Cuts at: 4253 5401 5600 6267 4253 Size: 1148 199 667 7401 Fragments arranged by size: 7401 1148 667 199 NheI G'CTAG_C Cuts at: 1052 3451 1052 Size: 2399 7016 NotI GC'GGCC_GC Cuts at: 6862 6862 Size: 9415 NsiI A_TGCA'T Cuts at: 4624 4624 Size: 9415 NspI r_CATG'y Cuts at: 1132 1229 3316 3433 5012 6773 8318 1132 Size: 97 2087 117 1579 1761 1545 2229 Fragments arranged by size: 2229 2087 1761 1579 1545 117 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9415 PciI A'CATG_T Cuts at: 8314 8314 Size: 9415 PflMI CCAn_nnn'nTGG Cuts at: 1108 2911 5680 1108 Size: 1803 2769 4843 Fragments arranged by size: 4843 2769 1803 PfoI T'CCnGG_A Cuts at: 3630 4353 3630 Size: 723 8692 PmeI GTTT'AAAC Cuts at: 6719 6719 Size: 9415 PmlI CAC'GTG Cuts at: 4057 5071 4057 Size: 1014 8401 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7484 7516 8997 9029 7484 Size: 32 1481 32 7870 Fragments arranged by size: 7870 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3651 5056 3651 Size: 1405 8010 PshAI GACnn'nnGTC Cuts at: 1613 6007 6581 1613 Size: 4394 574 4447 Fragments arranged by size: 4447 4394 574 PsiI TTA'TAA Cuts at: 6985 6985 Size: 9415 PspOMI G'GGCC_C Cuts at: 4234 4512 4234 Size: 278 9137 PspXI vC'TCGA_Gb Cuts at: 5485 5864 5485 Size: 379 9036 PstI C_TGCA'G Cuts at: 830 3882 4204 4777 6767 830 Size: 3052 322 573 1990 3478 Fragments arranged by size: 3478 3052 1990 573 322 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9415 PvuII CAG'CTG Cuts at: 2365 2824 2986 4165 4199 4883 2365 Size: 459 162 1179 34 684 6897 Fragments arranged by size: 6897 1179 684 459 162 34 SacI G_AGCT'C Cuts at: 2394 2394 Size: 9415 SacII CC_GC'GG Cuts at: 4494 4494 Size: 9415 SalI G'TCGA_C Cuts at: 1936 6757 1936 Size: 4821 4594 SanDI GG'GwC_CC Cuts at: 3651 3651 Size: 9415 SbfI CC_TGCA'GG Cuts at: 6767 6767 Size: 9415 ScaI AGT'ACT Cuts at: 7663 9375 7663 Size: 1712 7703 SexAI A'CCwGG_T Cuts at: 3171 3171 Size: 9415 SfcI C'TryA_G Cuts at: 826 1045 3878 4200 4773 4984 6763 7898 8579 Size: 219 2833 322 573 211 1779 1135 681 Cuts at: 8579 8770 826 Size: 191 1471 Fragments arranged by size: 2833 1779 1471 1135 681 573 322 219 211 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4474 4474 Size: 9415 SfoI GGC'GCC Cuts at: 3338 4384 5458 6100 6405 3338 Size: 1046 1074 642 305 6348 Fragments arranged by size: 6348 1074 1046 642 305 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9415 SgrAI Cr'CCGG_yG Cuts at: 1700 2265 1700 Size: 565 8850 SmaI CCC'GGG Cuts at: 1872 2497 2690 5379 6093 6334 6428 6708 6742 Size: 625 193 2689 714 241 94 280 34 Cuts at: 6742 1872 Size: 4545 Fragments arranged by size: 4545 2689 714 625 280 241 193 94 34 SmlI C'TyrA_G Cuts at: 820 1017 1943 3497 4530 5126 5485 5786 5864 Size: 197 926 1554 1033 596 359 301 78 Cuts at: 5864 6621 7519 8420 8682 820 Size: 757 898 901 262 1553 Fragments arranged by size: 1554 1553 1033 926 901 898 757 596 359 301 262 197 78 SnaBI TAC'GTA Cuts at: 493 7206 493 Size: 6713 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9415 SphI G_CATG'C Cuts at: 1132 6773 1132 Size: 5641 3774 SspI AAT'ATT Cuts at: 5 52 3400 7339 5 Size: 47 3348 3939 2081 Fragments arranged by size: 3939 3348 2081 47 StuI AGG'CCT Cuts at: 1517 3260 4468 4708 6672 1517 Size: 1743 1208 240 1964 4260 Fragments arranged by size: 4260 1964 1743 1208 240 StyI C'CwwG_G Cuts at: 513 1065 2173 2934 3525 4230 4631 5250 5452 Size: 552 1108 761 591 705 401 619 202 Cuts at: 5452 5899 5962 6228 6375 6854 513 Size: 447 63 266 147 479 3074 Fragments arranged by size: 3074 1108 761 705 619 591 552 479 447 401 266 202 147 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2149 4876 4958 5081 6433 6577 7442 Size: 1255 569 2727 82 123 1352 144 865 Cuts at: 7442 7459 7612 7797 8242 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2727 1498 1352 1255 865 569 445 185 153 144 123 82 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3432 3660 7661 Size: 274 80 33 51 1443 1455 228 4001 Cuts at: 7661 9373 96 Size: 1712 138 Fragments arranged by size: 4001 1712 1455 1443 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2387 2419 4046 4078 4561 4593 5621 5653 9375 Size: 32 1627 32 483 32 1028 32 3722 Cuts at: 9375 9407 2387 Size: 32 2395 Fragments arranged by size: 3722 2395 1627 1028 483 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2704 1316 Size: 1388 8027 XbaI T'CTAG_A Cuts at: 6751 6751 Size: 9415 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2578 3711 4375 6622 2578 Size: 1133 664 2247 5371 Fragments arranged by size: 5371 2247 1133 664 XhoI C'TCGA_G Cuts at: 1943 5485 5864 1943 Size: 3542 379 5494 Fragments arranged by size: 5494 3542 379 XmaI C'CCGG_G Cuts at: 1870 2495 2688 5377 6091 6332 6426 6706 6740 Size: 625 193 2689 714 241 94 280 34 Cuts at: 6740 1870 Size: 4545 Fragments arranged by size: 4545 2689 714 625 280 241 193 94 34 XmnI GAAnn'nnTTC Cuts at: 3122 7544 3122 Size: 4422 4993 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6160 7223 276 Size: 53 83 186 936 4626 1063 2468 Fragments arranged by size: 4626 2468 1063 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI BaeI BsiWI BstZ17I FseI FspAI NruI PacI PsrI RsrII SapI SrfI SwaI