(Circular) (Six-base) MAPSORT of: pFN21AA0968.seq Check: 8034 from: 1 to: 6139 pFN21AA0968 6139 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2027 2027 Size: 6139 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3949 278 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3482 1937 Size: 1545 4594 Acc65I G'GTAC_C Cuts at: 2631 3460 2631 Size: 829 5310 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2225 2432 2894 2921 4202 5586 5854 1632 Size: 593 207 462 27 1281 1384 268 1917 Fragments arranged by size: 1917 1384 1281 593 462 268 207 27 AflII C'TTAA_G Cuts at: 820 1017 2801 820 Size: 197 1784 4158 Fragments arranged by size: 4158 1784 197 AflIII A'CryG_T Cuts at: 1203 5038 6015 1203 Size: 3835 977 1327 Fragments arranged by size: 3835 1327 977 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 4868 4868 Size: 6139 AleI CACnn'nnGTG Cuts at: 1166 3035 1166 Size: 1869 4270 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3248 1184 Size: 2064 4075 AlwNI CAG_nnn'CTG Cuts at: 2173 2346 2362 3316 3882 5454 2173 Size: 173 16 954 566 1572 2858 Fragments arranged by size: 2858 1572 954 566 173 16 ApaLI G'TGCA_C Cuts at: 2399 3233 4196 5352 2399 Size: 834 963 1156 3186 Fragments arranged by size: 3186 1156 963 834 ApoI r'AATT_y Cuts at: 1493 3448 3674 3826 5935 1493 Size: 1955 226 152 2109 1697 Fragments arranged by size: 2109 1955 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 2313 3464 1870 Size: 73 370 1151 4545 Fragments arranged by size: 4545 1151 370 73 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2450 2631 2687 3369 3418 Size: 325 217 627 663 181 56 682 49 Cuts at: 3418 3460 4915 618 Size: 42 1455 1842 Fragments arranged by size: 1842 1455 682 663 627 325 217 181 56 49 42 BanII G_rGCy'C Cuts at: 1391 3458 1391 Size: 2067 4072 BbeI G_GCGC'C Cuts at: 2691 3422 2691 Size: 731 5408 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3033 928 Size: 971 1134 4034 Fragments arranged by size: 4034 1134 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4330 4364 1903 Size: 34 2393 34 3678 Fragments arranged by size: 3678 2393 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2360 4031 5241 2360 Size: 1671 1210 3258 Fragments arranged by size: 3258 1671 1210 BclI T'GATC_A Cuts at: 1548 2745 1548 Size: 1197 4942 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3381 4750 136 Size: 107 122 71 631 605 1709 1369 1525 Fragments arranged by size: 1709 1525 1369 631 605 122 107 71 BglII A'GATC_T Cuts at: 3410 6134 3410 Size: 2724 3415 BlpI GC'TnA_GC Cuts at: 3556 3556 Size: 6139 Bme1580I G_kGCm'C Cuts at: 1790 2403 3237 4200 5356 1790 Size: 613 834 963 1156 2573 Fragments arranged by size: 2573 1156 963 834 613 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3340 3870 4828 Size: 416 53 83 399 24 1923 530 958 Cuts at: 4828 442 Size: 1753 Fragments arranged by size: 1923 1753 958 530 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2930 2930 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 2384 4799 1239 Size: 956 189 2415 2579 Fragments arranged by size: 2579 2415 956 189 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2429 4264 5129 5427 2429 Size: 1835 865 298 3141 Fragments arranged by size: 3141 1835 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2154 2852 3350 4802 882 Size: 617 655 698 498 1452 2219 Fragments arranged by size: 2219 1452 698 655 617 498 BsaAI yAC'GTr Cuts at: 493 1204 3930 493 Size: 711 2726 2702 Fragments arranged by size: 2726 2702 711 BsaBI GATnn'nnATC Cuts at: 6133 6133 Size: 6139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2688 3419 3946 4328 Size: 53 83 186 936 1155 731 527 382 Cuts at: 4328 4963 275 Size: 635 1451 Fragments arranged by size: 1451 1155 936 731 635 527 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2945 4572 4970 5244 5391 5802 2945 Size: 1627 398 274 147 411 3282 Fragments arranged by size: 3282 1627 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5914 Fragments arranged by size: 5914 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 3368 1186 Size: 1075 1107 3957 Fragments arranged by size: 3957 1107 1075 BseYI C'CCAG_C Cuts at: 5342 5838 5342 Size: 496 5643 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2005 2360 1163 Size: 573 269 355 4942 Fragments arranged by size: 4942 573 355 269 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3589 4350 5378 1540 Size: 275 186 1588 761 1028 2301 Fragments arranged by size: 2301 1588 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2403 3005 3237 4200 4285 5356 2403 Size: 602 232 963 85 1071 3186 Fragments arranged by size: 3186 1071 963 602 232 85 BsiWI C'GTAC_G Cuts at: 2571 2571 Size: 6139 BsmI GAATG_Cn' Cuts at: 3650 3743 3650 Size: 93 6046 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2403 3005 3237 3458 4200 4285 5356 Size: 399 613 602 232 221 742 85 1071 Cuts at: 5356 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 613 602 399 232 221 85 BspEI T'CCGG_A Cuts at: 2945 5802 2945 Size: 2857 3282 BspHI T'CATG_A Cuts at: 4028 5758 4028 Size: 1730 4409 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2027 3494 844 Size: 1183 1467 3489 Fragments arranged by size: 3489 1467 1183 BsrBI CCG'CTC Cuts at: 1394 1823 2826 4026 1394 Size: 429 1003 1200 3507 Fragments arranged by size: 3507 1200 1003 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3097 4634 4808 65 Size: 1183 1849 1537 174 1396 Fragments arranged by size: 1849 1537 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2684 4783 4970 1700 Size: 110 874 2099 187 2869 Fragments arranged by size: 2869 2099 874 187 110 BsrGI T'GTAC_A Cuts at: 96 2613 96 Size: 2517 3622 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6139 BssSI C'ACGA_G Cuts at: 4199 5211 4199 Size: 1012 5127 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6139 BstBI TT'CG_AA Cuts at: 3144 4943 3144 Size: 1799 4340 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6139 BstXI CCAn_nnnn'nTGG Cuts at: 2491 2926 3413 2491 Size: 435 487 5217 Fragments arranged by size: 5217 487 435 BstYI r'GATC_y Cuts at: 1972 2358 2740 2814 3410 3469 3836 4221 4238 Size: 386 382 74 596 59 367 385 17 Cuts at: 4238 5690 5776 5788 6134 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 596 386 385 382 367 346 86 74 59 17 12 Bsu36I CC'TnA_GG Cuts at: 3150 3150 Size: 6139 BtgI C'CryG_G Cuts at: 513 1065 2004 2832 2862 513 Size: 552 939 828 30 3790 Fragments arranged by size: 3790 939 828 552 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2688 2991 3274 487 Size: 683 56 1462 303 283 3352 Fragments arranged by size: 3352 1462 683 303 283 56 BtsI GCAGTG_nn' Cuts at: 2377 2463 3655 4449 4469 2377 Size: 86 1192 794 20 4047 Fragments arranged by size: 4047 1192 794 86 20 ClaI AT'CG_AT Cuts at: 1554 3190 1554 Size: 1636 4503 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2110 2481 Size: 54 1425 50 275 18 177 103 371 Cuts at: 2481 2928 3586 4475 6104 8 Size: 447 658 889 1629 43 Fragments arranged by size: 1629 1425 889 658 447 371 275 177 103 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3586 1537 Size: 275 1774 4090 Fragments arranged by size: 4090 1774 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 2054 2948 2966 4069 1353 Size: 678 23 894 18 1103 3423 Fragments arranged by size: 3423 1103 894 678 23 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2348 4726 5100 5246 387 Size: 1064 138 759 2378 374 146 1280 Fragments arranged by size: 2378 1280 1064 759 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2225 2384 2432 2894 2921 4202 Size: 393 563 30 159 48 462 27 1281 Cuts at: 4202 4799 5586 5854 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1281 787 597 563 462 393 268 159 48 30 27 EcoNI CCTnn'n_nnAGG Cuts at: 3170 3170 Size: 6139 EcoO109I rG'GnC_Cy Cuts at: 2407 2566 3154 3175 2407 Size: 159 588 21 5371 Fragments arranged by size: 5371 588 159 21 EcoRI G'AATT_C Cuts at: 3448 3826 3448 Size: 378 5761 EcoRV GAT'ATC Cuts at: 2305 2540 2305 Size: 235 5904 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2089 2089 Size: 6139 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 2691 3422 5286 1678 Size: 1013 731 1864 2531 Fragments arranged by size: 2531 1864 1013 731 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 2687 2719 3089 Size: 32 135 32 653 32 430 32 370 Cuts at: 3089 3121 4780 4812 4854 4886 1373 Size: 32 1659 32 42 32 2626 Fragments arranged by size: 2626 1659 653 430 370 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3483 3729 669 Size: 988 281 1545 246 3079 Fragments arranged by size: 3079 1545 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6139 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KasI G'GCGC_C Cuts at: 2687 3418 2687 Size: 731 5408 KpnI G_GTAC'C Cuts at: 2635 3464 2635 Size: 829 5310 MfeI C'AATT_G Cuts at: 3738 3738 Size: 6139 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2248 3330 5253 5437 1305 Size: 943 1082 1923 184 2007 Fragments arranged by size: 2007 1923 1082 943 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2112 2483 2930 10 Size: 54 1425 343 177 103 371 447 3219 Fragments arranged by size: 3219 1425 447 371 343 177 103 54 MslI CAynn'nnrTG Cuts at: 518 1166 2489 2921 3035 3116 4097 4456 4615 Size: 648 1323 432 114 81 981 359 159 Cuts at: 4615 518 Size: 2042 Fragments arranged by size: 2042 1323 981 648 432 359 159 114 81 MspA1I CmG'CkG Cuts at: 1606 2789 3073 3555 4232 5380 5625 1606 Size: 1183 284 482 677 1148 245 2120 Fragments arranged by size: 2120 1183 1148 677 482 284 245 NaeI GCC'GGC Cuts at: 2686 2686 Size: 6139 NarI GG'CG_CC Cuts at: 2688 3419 2688 Size: 731 5408 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4648 Fragments arranged by size: 4648 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6139 NgoMIV G'CCGG_C Cuts at: 2684 2684 Size: 6139 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 3586 3586 Size: 6139 NspI r_CATG'y Cuts at: 1132 1229 2848 3497 5042 1132 Size: 97 1619 649 1545 2229 Fragments arranged by size: 2229 1619 1545 649 97 PasI CC'CwG_GG Cuts at: 1348 3157 1348 Size: 1809 4330 PciI A'CATG_T Cuts at: 5038 5038 Size: 6139 PflMI CCAn_nnn'nTGG Cuts at: 1108 2389 3268 1108 Size: 1281 879 3979 Fragments arranged by size: 3979 1281 879 PfoI T'CCnGG_A Cuts at: 2361 2533 3215 2361 Size: 172 682 5285 Fragments arranged by size: 5285 682 172 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 5721 5753 4208 Size: 32 1481 32 4594 Fragments arranged by size: 4594 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2407 2566 2407 Size: 159 5980 PshAI GACnn'nnGTC Cuts at: 1613 3362 1613 Size: 1749 4390 PsiI TTA'TAA Cuts at: 3056 3709 3056 Size: 653 5486 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2624 2656 2624 Size: 32 6107 PstI C_TGCA'G Cuts at: 830 3491 830 Size: 2661 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 PvuII CAG'CTG Cuts at: 2789 3073 2789 Size: 284 5855 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SapI GCTCTTCn'nnn_ Cuts at: 2054 2966 2054 Size: 912 5227 SbfI CC_TGCA'GG Cuts at: 3491 3491 Size: 6139 ScaI AGT'ACT Cuts at: 4387 6099 4387 Size: 1712 4427 SexAI A'CCwGG_T Cuts at: 2274 3165 2274 Size: 891 5248 SfcI C'TryA_G Cuts at: 826 1045 3487 4622 5303 5494 826 Size: 219 2442 1135 681 191 1471 Fragments arranged by size: 2442 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2689 3420 2689 Size: 731 5408 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6139 SmaI CCC'GGG Cuts at: 1872 2315 3466 1872 Size: 443 1151 4545 Fragments arranged by size: 4545 1151 443 SmlI C'TyrA_G Cuts at: 820 1017 1943 2444 2801 4243 5144 5406 820 Size: 197 926 501 357 1442 901 262 1553 Fragments arranged by size: 1553 1442 926 901 501 357 262 197 SnaBI TAC'GTA Cuts at: 493 3930 493 Size: 3437 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 2848 3497 1132 Size: 1716 649 3774 Fragments arranged by size: 3774 1716 649 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6139 StyI C'CwwG_G Cuts at: 513 1065 2004 2736 3578 513 Size: 552 939 732 842 3074 Fragments arranged by size: 3074 939 842 732 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4166 4183 4336 4521 4966 325 Size: 1255 2586 17 153 185 445 1498 Fragments arranged by size: 2586 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2613 4385 6097 Size: 274 80 33 51 1443 636 1772 1712 Cuts at: 6097 96 Size: 138 Fragments arranged by size: 1772 1712 1443 636 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6099 6131 6099 Size: 32 6107 Tth111I GACn'n_nGTC Cuts at: 1316 2276 2720 1316 Size: 960 444 4735 Fragments arranged by size: 4735 960 444 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2709 2709 Size: 6139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 2313 3464 1870 Size: 443 1151 4545 Fragments arranged by size: 4545 1151 443 XmnI GAAnn'nnTTC Cuts at: 2879 4268 2879 Size: 1389 4750 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3947 276 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsmBI BstZ17I DraIII EcoICRI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SfiI SrfI SwaI