(Circular) (Six-base) MAPSORT of: pFN21AA0938.seq Check: 9810 from: 1 to: 10231 pFN21AA0938 10231 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4262 4262 Size: 10231 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6253 8041 278 Size: 53 83 186 936 4717 1788 2468 Fragments arranged by size: 4717 2468 1788 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2710 2846 7574 1937 Size: 773 136 4728 4594 Fragments arranged by size: 4728 4594 773 136 Acc65I G'GTAC_C Cuts at: 2804 7552 2804 Size: 4748 5483 AclI AA'CG_TT Cuts at: 1563 8358 8731 1563 Size: 6795 373 3063 Fragments arranged by size: 6795 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 2388 2439 2741 3432 3603 4802 5169 Size: 422 334 51 302 691 171 1199 367 Cuts at: 5169 5393 5718 5804 7190 7295 8294 9678 9946 Size: 224 325 86 1386 105 999 1384 268 Cuts at: 9946 1632 Size: 1917 Fragments arranged by size: 1917 1386 1384 1199 999 691 422 367 334 325 302 268 224 171 105 86 51 AflII C'TTAA_G Cuts at: 820 1017 4477 820 Size: 197 3460 6574 Fragments arranged by size: 6574 3460 197 AflIII A'CryG_T Cuts at: 1203 2379 2889 2934 9130 10107 1203 Size: 1176 510 45 6196 977 1327 Fragments arranged by size: 6196 1327 1176 977 510 45 AgeI A'CCGG_T Cuts at: 5925 9062 5925 Size: 3137 7094 AhdI GACnn_n'nnGTC Cuts at: 6655 8960 6655 Size: 2305 7926 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10231 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2031 1184 Size: 847 9384 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3203 3235 3203 Size: 32 10199 AlwNI CAG_nnn'CTG Cuts at: 2239 2412 4185 4699 5759 5942 6056 7974 9546 Size: 173 1773 514 1060 183 114 1918 1572 Cuts at: 9546 2239 Size: 2924 Fragments arranged by size: 2924 1918 1773 1572 1060 514 183 173 114 ApaI G_GGCC'C Cuts at: 4944 4944 Size: 10231 ApaLI G'TGCA_C Cuts at: 3528 4191 8288 9444 3528 Size: 663 4097 1156 4315 Fragments arranged by size: 4315 4097 1156 663 ApoI r'AATT_y Cuts at: 1493 2587 3789 4915 5383 7540 7766 7918 10027 Size: 1094 1202 1126 468 2157 226 152 2109 Cuts at: 10027 1493 Size: 1697 Fragments arranged by size: 2157 2109 1697 1202 1126 1094 468 226 152 AseI AT'TA_AT Cuts at: 160 1032 8785 160 Size: 872 7753 1606 Fragments arranged by size: 7753 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10231 AvaI C'yCGr_G Cuts at: 1870 1943 2833 3090 3419 3542 4069 4937 7453 Size: 73 890 257 329 123 527 868 2516 Cuts at: 7453 7556 1870 Size: 103 4545 Fragments arranged by size: 4545 2516 890 868 527 329 257 123 103 73 BamHI G'GATC_C Cuts at: 4214 6194 7561 7928 4214 Size: 1980 1367 367 6517 Fragments arranged by size: 6517 1980 1367 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2804 3499 4262 7552 9007 Size: 325 217 627 1017 695 763 3290 1455 Cuts at: 9007 618 Size: 1842 Fragments arranged by size: 3290 1842 1455 1017 763 695 627 325 217 BanII G_rGCy'C Cuts at: 1391 4622 4910 4944 5069 5796 6308 6732 6773 Size: 3231 288 34 125 727 512 424 41 Cuts at: 6773 7277 7301 7550 1391 Size: 504 24 249 4072 Fragments arranged by size: 4072 3231 727 512 504 424 288 249 125 41 34 24 BbeI G_GCGC'C Cuts at: 3503 3503 Size: 10231 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2310 2538 2603 2931 3395 3420 5464 Size: 971 411 228 65 328 464 25 2044 Cuts at: 5464 7037 7196 7503 928 Size: 1573 159 307 3656 Fragments arranged by size: 3656 2044 1573 971 464 411 328 307 228 159 65 25 BbvCI CC'TCA_GC Cuts at: 3275 4643 5760 5840 3275 Size: 1368 1117 80 7666 Fragments arranged by size: 7666 1368 1117 80 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 8422 8456 1903 Size: 34 50 34 6401 34 3678 Fragments arranged by size: 6401 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4233 4573 6380 8123 9333 4233 Size: 340 1807 1743 1210 5131 Fragments arranged by size: 5131 1807 1743 1210 340 BclI T'GATC_A Cuts at: 1548 3738 5860 1548 Size: 2190 2122 5919 Fragments arranged by size: 5919 2190 2122 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3508 3963 4653 Size: 107 122 71 631 605 1836 455 690 Cuts at: 4653 5399 5965 8842 136 Size: 746 566 2877 1525 Fragments arranged by size: 2877 1836 1525 746 690 631 605 566 455 122 107 71 BglII A'GATC_T Cuts at: 3611 4724 5443 10226 3611 Size: 1113 719 4783 3616 Fragments arranged by size: 4783 3616 1113 719 BlpI GC'TnA_GC Cuts at: 2257 4415 5704 5955 7276 7648 2257 Size: 2158 1289 251 1321 372 4840 Fragments arranged by size: 4840 2158 1321 1289 372 251 Bme1580I G_kGCm'C Cuts at: 1790 3532 4195 4944 8292 9448 1790 Size: 1742 663 749 3348 1156 2573 Fragments arranged by size: 3348 2573 1742 1156 749 663 BmgBI CAC'GTC Cuts at: 1318 7035 1318 Size: 5717 4514 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3791 6728 7962 Size: 416 53 83 399 24 2374 2937 1234 Cuts at: 7962 8920 442 Size: 958 1753 Fragments arranged by size: 2937 2374 1753 1234 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 6324 1056 Size: 5268 4963 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2218 3313 4746 6746 2218 Size: 1095 1433 2000 5703 Fragments arranged by size: 5703 2000 1433 1095 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2663 2749 3490 4388 4480 4830 5054 5852 Size: 1424 86 741 898 92 350 224 798 Cuts at: 5852 7205 8891 1239 Size: 1353 1686 2579 Fragments arranged by size: 2579 1686 1424 1353 898 798 741 350 224 92 86 Bpu10I CC'TnA_GC Cuts at: 3275 4643 5278 5760 5840 3275 Size: 1368 635 482 80 7666 Fragments arranged by size: 7666 1368 635 482 80 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2599 5968 8356 9221 9519 2599 Size: 3369 2388 865 298 3311 Fragments arranged by size: 3369 3311 2388 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2116 3176 3305 4205 4538 8894 882 Size: 617 617 1060 129 900 333 4356 2219 Fragments arranged by size: 4356 2219 1060 900 617 617 333 129 BsaAI yAC'GTr Cuts at: 493 1204 8022 493 Size: 711 6818 2702 Fragments arranged by size: 6818 2702 711 BsaBI GATnn'nnATC Cuts at: 10225 10225 Size: 10231 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3500 6250 8038 8420 Size: 53 83 186 936 1967 2750 1788 382 Cuts at: 8420 9055 275 Size: 635 1451 Fragments arranged by size: 2750 1967 1788 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2034 4382 5883 5925 8664 9062 9336 9483 9894 Size: 2348 1501 42 2739 398 274 147 411 Cuts at: 9894 2034 Size: 2371 Fragments arranged by size: 2739 2371 2348 1501 411 398 274 147 42 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2369 2399 4361 4391 5599 Size: 30 165 30 966 30 1962 30 1208 Cuts at: 5599 5629 1178 Size: 30 5780 Fragments arranged by size: 5780 1962 1208 966 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2134 2438 2483 3975 5986 1186 Size: 948 304 45 1492 2011 5431 Fragments arranged by size: 5431 2011 1492 948 304 45 BseYI C'CCAG_C Cuts at: 2279 2412 2488 2881 3141 4016 4331 4501 4943 Size: 133 76 393 260 875 315 170 442 Cuts at: 4943 4969 5007 7300 9434 9930 2279 Size: 26 38 2293 2134 496 2580 Fragments arranged by size: 2580 2293 2134 875 496 442 393 315 260 170 133 76 38 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2252 4275 6724 7330 1163 Size: 573 516 2023 2449 606 4064 Fragments arranged by size: 4064 2449 2023 606 573 516 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7681 8442 9470 1540 Size: 275 186 5680 761 1028 2301 Fragments arranged by size: 5680 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3532 4195 4622 5416 5796 6308 6732 7277 7460 Size: 663 427 794 380 512 424 545 183 Cuts at: 7460 8292 8377 9448 3532 Size: 832 85 1071 4315 Fragments arranged by size: 4315 1071 832 794 663 545 512 427 424 380 183 85 BsmI GAATG_Cn' Cuts at: 3948 5464 5753 7742 7835 3948 Size: 1516 289 1989 93 6344 Fragments arranged by size: 6344 1989 1516 289 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6257 6257 Size: 10231 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3532 4195 4622 4910 4944 5069 5416 Size: 399 1742 663 427 288 34 125 347 Cuts at: 5416 5796 6308 6732 6773 7277 7301 7460 7550 Size: 380 512 424 41 504 24 159 90 Cuts at: 7550 8292 8377 9448 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1742 1071 742 663 512 504 427 424 399 380 347 288 159 125 90 85 41 34 24 BspEI T'CCGG_A Cuts at: 2034 9894 2034 Size: 7860 2371 BspHI T'CATG_A Cuts at: 4704 8120 9850 4704 Size: 3416 1730 5085 Fragments arranged by size: 5085 3416 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4262 7586 844 Size: 3418 3324 3489 Fragments arranged by size: 3489 3418 3324 BsrBI CCG'CTC Cuts at: 1394 1823 2197 8118 1394 Size: 429 374 5921 3507 Fragments arranged by size: 5921 3507 429 374 BsrDI GCAATG_nn' Cuts at: 65 1248 6315 8726 8900 65 Size: 1183 5067 2411 174 1396 Fragments arranged by size: 5067 2411 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5925 8875 9062 1700 Size: 110 4115 2950 187 2869 Fragments arranged by size: 4115 2950 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10231 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10231 BssSI C'ACGA_G Cuts at: 4535 4615 4685 5046 8291 9303 4535 Size: 80 70 361 3245 1012 5463 Fragments arranged by size: 5463 3245 1012 361 80 70 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 10231 BstBI TT'CG_AA Cuts at: 4249 6952 9035 4249 Size: 2703 2083 5445 Fragments arranged by size: 5445 2703 2083 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10231 BstXI CCAn_nnnn'nTGG Cuts at: 5015 5032 5690 5015 Size: 17 658 9556 Fragments arranged by size: 9556 658 17 BstYI r'GATC_y Cuts at: 1972 3611 3971 4214 4724 5443 5865 6194 6347 Size: 1639 360 243 510 719 422 329 153 Cuts at: 6347 7150 7237 7405 7561 7928 8313 8330 9782 Size: 803 87 168 156 367 385 17 1452 Cuts at: 9782 9868 9880 10226 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1639 1452 803 719 510 422 385 367 360 346 329 243 168 156 153 87 86 17 12 BstZ17I GTA'TAC Cuts at: 2847 2847 Size: 10231 Bsu36I CC'TnA_GG Cuts at: 3327 3664 7290 3327 Size: 337 3626 6268 Fragments arranged by size: 6268 3626 337 BtgI C'CryG_G Cuts at: 513 1065 2127 3364 7116 7229 7268 513 Size: 552 1062 1237 3752 113 39 3476 Fragments arranged by size: 3752 3476 1237 1062 552 113 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9492 Fragments arranged by size: 9492 683 56 BtsI GCAGTG_nn' Cuts at: 2806 2929 3172 3643 3754 4408 5527 6124 6712 Size: 123 243 471 111 654 1119 597 588 Cuts at: 6712 7197 7747 8541 8561 2806 Size: 485 550 794 20 4476 Fragments arranged by size: 4476 1119 794 654 597 588 550 485 471 243 123 111 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10231 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4255 4290 6277 6312 529 Size: 35 3691 35 1987 35 4448 Fragments arranged by size: 4448 3691 1987 35 35 35 DraI TTT'AAA Cuts at: 7535 7535 Size: 10231 DraIII CAC_nnn'GTG Cuts at: 3121 3121 Size: 10231 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9238 809 Size: 507 7922 1802 Fragments arranged by size: 7922 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3955 5293 5966 Size: 54 1425 50 275 18 2125 1338 673 Cuts at: 5966 6112 7678 8567 10196 8 Size: 146 1566 889 1629 43 Fragments arranged by size: 2125 1629 1566 1425 1338 889 673 275 146 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7678 1537 Size: 275 5866 4090 Fragments arranged by size: 5866 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2323 2415 2589 3469 4442 4770 4987 5107 Size: 970 92 174 880 973 328 217 120 Cuts at: 5107 5634 5780 6004 8161 1353 Size: 527 146 224 2157 3423 Fragments arranged by size: 3423 2157 973 970 880 527 328 224 217 174 146 120 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8818 9192 9338 387 Size: 1064 138 7229 374 146 1280 Fragments arranged by size: 7229 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4620 5794 6306 6730 7275 4620 Size: 1174 512 424 545 7576 Fragments arranged by size: 7576 1174 545 512 424 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2388 2439 2663 2741 2749 3432 Size: 393 422 334 51 224 78 8 683 Cuts at: 3432 3490 3603 4388 4480 4802 4830 5054 5169 Size: 58 113 785 92 322 28 224 115 Cuts at: 5169 5393 5718 5804 5852 7190 7205 7295 8294 Size: 224 325 86 48 1338 15 90 999 Cuts at: 8294 8891 9678 9946 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1338 999 787 785 683 597 422 393 334 325 322 268 224 224 224 115 113 92 90 86 78 58 51 48 28 15 8 EcoNI CCTnn'n_nnAGG Cuts at: 3957 3957 Size: 10231 EcoO109I rG'GnC_Cy Cuts at: 5836 5836 Size: 10231 EcoRI G'AATT_C Cuts at: 5383 7540 7918 5383 Size: 2157 378 7696 Fragments arranged by size: 7696 2157 378 EcoRV GAT'ATC Cuts at: 2980 6784 6947 2980 Size: 3804 163 6264 Fragments arranged by size: 6264 3804 163 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3633 4158 3633 Size: 525 9706 FspI TGC'GCA Cuts at: 2135 8737 2135 Size: 6602 3629 FspAI rTGC'GCAy Cuts at: 2135 2135 Size: 10231 HaeII r_GCGC'y Cuts at: 1678 2203 3503 4069 4185 9378 1678 Size: 525 1300 566 116 5193 2531 Fragments arranged by size: 5193 2531 1300 566 525 116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2303 2335 2348 2380 2443 Size: 32 135 32 731 32 13 32 63 Cuts at: 2443 2475 2588 2620 2759 2791 2856 2888 3154 Size: 32 113 32 139 32 65 32 266 Cuts at: 3154 3186 4220 4252 4361 4393 4716 4748 5522 Size: 32 1034 32 109 32 323 32 774 Cuts at: 5522 5554 5687 5719 6158 6190 6505 6537 6641 Size: 32 133 32 439 32 315 32 104 Cuts at: 6641 6673 7024 7056 7186 7218 8872 8904 8946 Size: 32 351 32 130 32 1654 32 42 Cuts at: 8946 8978 1373 Size: 32 2626 Fragments arranged by size: 2626 1654 1034 774 731 439 351 323 315 266 139 135 133 130 113 109 104 65 63 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 2711 4087 7575 7821 669 Size: 988 281 773 1376 3488 246 3079 Fragments arranged by size: 3488 3079 1376 988 773 281 246 HindIII A'AGCT_T Cuts at: 748 3438 3564 4474 748 Size: 2690 126 910 6505 Fragments arranged by size: 6505 2690 910 126 HpaI GTT'AAC Cuts at: 7821 7821 Size: 10231 KasI G'GCGC_C Cuts at: 3499 3499 Size: 10231 KpnI G_GTAC'C Cuts at: 2808 7556 2808 Size: 4748 5483 MfeI C'AATT_G Cuts at: 6630 6802 7830 6630 Size: 172 1028 9031 Fragments arranged by size: 9031 1028 172 MluI A'CGCG_T Cuts at: 10107 10107 Size: 10231 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2166 3264 4020 6376 9345 9529 1305 Size: 861 1098 756 2356 2969 184 2007 Fragments arranged by size: 2969 2356 2007 1098 861 756 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 8409 Fragments arranged by size: 8409 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2463 2720 3369 5366 6108 6416 7267 Size: 648 1297 257 649 1997 742 308 851 Cuts at: 7267 8189 8548 8707 518 Size: 922 359 159 2042 Fragments arranged by size: 2042 1997 1297 922 851 742 649 648 359 308 257 159 MspA1I CmG'CkG Cuts at: 1606 2011 3141 3274 4877 5147 5321 5782 7315 Size: 405 1130 133 1603 270 174 461 1533 Cuts at: 7315 7647 8324 9472 9717 1606 Size: 332 677 1148 245 2120 Fragments arranged by size: 2120 1603 1533 1148 1130 677 461 405 332 270 245 174 133 NarI GG'CG_CC Cuts at: 3500 3500 Size: 10231 NcoI C'CATG_G Cuts at: 513 1065 3364 7116 7229 7268 513 Size: 552 2299 3752 113 39 3476 Fragments arranged by size: 3752 3476 2299 552 113 39 NdeI CA'TA_TG Cuts at: 387 387 Size: 10231 NheI G'CTAG_C Cuts at: 1052 6320 1052 Size: 5268 4963 NotI GC'GGCC_GC Cuts at: 7678 7678 Size: 10231 NspI r_CATG'y Cuts at: 1132 1229 2748 2893 2938 3484 3884 7589 9134 Size: 97 1519 145 45 546 400 3705 1545 Cuts at: 9134 1132 Size: 2229 Fragments arranged by size: 3705 2229 1545 1519 546 400 145 97 45 PasI CC'CwG_GG Cuts at: 1348 2779 1348 Size: 1431 8800 PciI A'CATG_T Cuts at: 2889 2934 9130 2889 Size: 45 6196 3990 Fragments arranged by size: 6196 3990 45 PflMI CCAn_nnn'nTGG Cuts at: 1108 2727 1108 Size: 1619 8612 PfoI T'CCnGG_A Cuts at: 3467 5868 3467 Size: 2401 7830 PmeI GTTT'AAAC Cuts at: 7535 7535 Size: 10231 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8300 8332 9813 9845 8300 Size: 32 1481 32 8686 Fragments arranged by size: 8686 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10231 PsiI TTA'TAA Cuts at: 4472 7801 4472 Size: 3329 6902 PspOMI G'GGCC_C Cuts at: 4940 4940 Size: 10231 PspXI vC'TCGA_Gb Cuts at: 2833 4069 7453 2833 Size: 1236 3384 5611 Fragments arranged by size: 5611 3384 1236 PstI C_TGCA'G Cuts at: 830 4570 4876 5781 7583 830 Size: 3740 306 905 1802 3478 Fragments arranged by size: 3740 3478 1802 905 306 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10231 PvuII CAG'CTG Cuts at: 3141 3274 4877 5147 5782 7315 3141 Size: 133 1603 270 635 1533 6057 Fragments arranged by size: 6057 1603 1533 635 270 133 SacI G_AGCT'C Cuts at: 4622 5796 6308 6732 7277 4622 Size: 1174 512 424 545 7576 Fragments arranged by size: 7576 1174 545 512 424 SalI G'TCGA_C Cuts at: 1936 2709 7573 1936 Size: 773 4864 4594 Fragments arranged by size: 4864 4594 773 SapI GCTCTTCn'nnn_ Cuts at: 5107 5634 5780 5107 Size: 527 146 9558 Fragments arranged by size: 9558 527 146 SbfI CC_TGCA'GG Cuts at: 7583 7583 Size: 10231 ScaI AGT'ACT Cuts at: 8479 10191 8479 Size: 1712 8519 SfcI C'TryA_G Cuts at: 826 1045 4461 4566 4872 5142 5777 7579 8714 Size: 219 3416 105 306 270 635 1802 1135 Cuts at: 8714 9395 9586 826 Size: 681 191 1471 Fragments arranged by size: 3416 1802 1471 1135 681 635 306 270 219 191 105 SfiI GGCCn_nnn'nGGCC Cuts at: 3963 3963 Size: 10231 SfoI GGC'GCC Cuts at: 3501 3501 Size: 10231 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10231 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10231 SmaI CCC'GGG Cuts at: 1872 3092 3544 4939 7558 1872 Size: 1220 452 1395 2619 4545 Fragments arranged by size: 4545 2619 1395 1220 452 SmlI C'TyrA_G Cuts at: 820 1017 1943 2578 2833 4069 4477 5983 7453 Size: 197 926 635 255 1236 408 1506 1470 Cuts at: 7453 8335 9236 9498 820 Size: 882 901 262 1553 Fragments arranged by size: 1553 1506 1470 1236 926 901 882 635 408 262 255 197 SnaBI TAC'GTA Cuts at: 493 8022 493 Size: 7529 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10231 SphI G_CATG'C Cuts at: 1132 7589 1132 Size: 6457 3774 SspI AAT'ATT Cuts at: 5 52 8155 5 Size: 47 8103 2081 Fragments arranged by size: 8103 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 10231 StyI C'CwwG_G Cuts at: 513 1065 2163 2983 3364 3490 4266 6295 7116 Size: 552 1098 820 381 126 776 2029 821 Cuts at: 7116 7229 7268 7670 513 Size: 113 39 402 3074 Fragments arranged by size: 3074 2029 1098 821 820 776 552 402 381 126 113 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2480 2771 2945 4255 6559 7240 8258 Size: 1255 900 291 174 1310 2304 681 1018 Cuts at: 8258 8275 8428 8613 9058 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2304 1498 1310 1255 1018 900 681 445 291 185 174 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 8477 10189 96 Size: 274 80 33 51 1443 6500 1712 138 Fragments arranged by size: 6500 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5599 5631 10191 10223 5599 Size: 32 4560 32 5607 Fragments arranged by size: 5607 4560 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2602 3394 7004 1316 Size: 1286 792 3610 4543 Fragments arranged by size: 4543 3610 1286 792 XbaI T'CTAG_A Cuts at: 2497 6871 7133 7567 2497 Size: 4374 262 434 5161 Fragments arranged by size: 5161 4374 434 262 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3371 5513 6643 3371 Size: 2142 1130 6959 Fragments arranged by size: 6959 2142 1130 XhoI C'TCGA_G Cuts at: 1943 2833 4069 7453 1943 Size: 890 1236 3384 4721 Fragments arranged by size: 4721 3384 1236 890 XmaI C'CCGG_G Cuts at: 1870 3090 3542 4937 7556 1870 Size: 1220 452 1395 2619 4545 Fragments arranged by size: 4545 2619 1395 1220 452 XmnI GAAnn'nnTTC Cuts at: 5091 8360 5091 Size: 3269 6962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6251 8039 276 Size: 53 83 186 936 4717 1788 2468 Fragments arranged by size: 4717 2468 1788 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI FseI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PsrI RsrII SacII SanDI SexAI SrfI SwaI